Restriction map for
pFN21AB6004
(Circular) (Six-base) MAPSORT of: pFN21AB6004.seq Check: 2043 from: 1 to: 10639
pFN21AB6004 10639 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8449 278
Size: 53 83 186 936 6913 2468
Fragments arranged by size:
6913 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3367 7982 1937
Size: 1430 4615 4594
Fragments arranged by size:
4615 4594 1430
Acc65I G'GTAC_C
Cuts at: 7960 7960
Size: 10639
AclI AA'CG_TT
Cuts at: 1563 8766 9139 1563
Size: 7203 373 3063
Fragments arranged by size:
7203 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4092 5051 5075 5132 5541 5760 5814 7032
Size: 2460 959 24 57 409 219 54 1218
Cuts at: 7032 7290 7707 7731 7941 8702 10086 10354 1632
Size: 258 417 24 210 761 1384 268 1917
Fragments arranged by size:
2460 1917 1384 1218 959 761 417 409
268 258 219 210 57 54 24 24
AfeI AGC'GCT
Cuts at: 3877 3877
Size: 10639
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10442
AflIII A'CryG_T
Cuts at: 1203 3091 3546 5468 7853 9538 10515 1203
Size: 1888 455 1922 2385 1685 977 1327
Fragments arranged by size:
2385 1922 1888 1685 1327 977 455
AgeI A'CCGG_T
Cuts at: 9470 9470
Size: 10639
AhdI GACnn_n'nnGTC
Cuts at: 2912 2973 3949 9368 2912
Size: 61 976 5419 4183
Fragments arranged by size:
5419 4183 976 61
AleI CACnn'nnGTG
Cuts at: 1166 3283 1166
Size: 2117 8522
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 7256 1184
Size: 6072 4567
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7008 7040 7008
Size: 32 10607
AlwNI CAG_nnn'CTG
Cuts at: 5354 7010 8382 9954 5354
Size: 1656 1372 1572 6039
Fragments arranged by size:
6039 1656 1572 1372
ApaLI G'TGCA_C
Cuts at: 8696 9852 8696
Size: 1156 9483
ApoI r'AATT_y
Cuts at: 1493 2154 2700 2726 3576 3773 4163 4877 5535
Size: 661 546 26 850 197 390 714 658
Cuts at: 5535 5718 5862 5945 6311 6368 6623 6916 6922
Size: 183 144 83 366 57 255 293 6
Cuts at: 6922 7877 7948 8174 8326 10435 1493
Size: 955 71 226 152 2109 1697
Fragments arranged by size:
2109 1697 955 850 714 661 658 546
390 366 293 255 226 197 183 152
144 83 71 57 26 6
AseI AT'TA_AT
Cuts at: 160 1032 2519 3026 3075 6947 9193 160
Size: 872 1487 507 49 3872 2246 1606
Fragments arranged by size:
3872 2246 1606 1487 872 507 49
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10639
AvaI C'yCGr_G
Cuts at: 1870 1943 2028 7964 1870
Size: 73 85 5936 4545
Fragments arranged by size:
5936 4545 85 73
AvrII C'CTAG_G
Cuts at: 3482 3482
Size: 10639
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5838 5871 5838
Size: 33 10606
BamHI G'GATC_C
Cuts at: 3054 7969 8336 3054
Size: 4915 367 5357
Fragments arranged by size:
5357 4915 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2553 7180 7960 9415 618
Size: 325 217 627 766 4627 780 1455 1842
Fragments arranged by size:
4627 1842 1455 780 766 627 325 217
BanII G_rGCy'C
Cuts at: 1391 7958 1391
Size: 6567 4072
BbeI G_GCGC'C
Cuts at: 2557 2557
Size: 10639
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2466 2808 3466 5888 7194 7287 7712
Size: 971 567 342 658 2422 1306 93 425
Cuts at: 7712 7719 928
Size: 7 3848
Fragments arranged by size:
3848 2422 1306 971 658 567 425 342
93 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5579 5613 8830 8864 1903
Size: 34 3642 34 3217 34 3678
Fragments arranged by size:
3678 3642 3217 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3396 8531 9741 3396
Size: 5135 1210 4294
Fragments arranged by size:
5135 4294 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 10639
BfrBI ATG'CAT
Cuts at: 3541 3541
Size: 10639
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 9250 136
Size: 107 122 71 631 605 7578 1525
Fragments arranged by size:
7578 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7021 7516 10634 7021
Size: 495 3118 7026
Fragments arranged by size:
7026 3118 495
BlpI GC'TnA_GC
Cuts at: 6537 8056 6537
Size: 1519 9120
Bme1580I G_kGCm'C
Cuts at: 1790 6270 7185 8700 9856 1790
Size: 4480 915 1515 1156 2573
Fragments arranged by size:
4480 2573 1515 1156 915
BmgBI CAC'GTC
Cuts at: 1318 7856 1318
Size: 6538 4101
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3471 4554 4889
Size: 416 53 83 399 24 2054 1083 335
Cuts at: 4889 5880 8370 9328 442
Size: 991 2490 958 1753
Fragments arranged by size:
2490 2054 1753 1083 991 958 416 399
335 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10639
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2440 2997 2440
Size: 557 10082
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2070 3974 9299 1239
Size: 831 1904 5325 2579
Fragments arranged by size:
5325 2579 1904 831
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2410 3490 5496 8764 9629 9927 2410
Size: 1080 2006 3268 865 298 3122
Fragments arranged by size:
3268 3122 2006 1080 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2005 2123 3156 3531 9302 882
Size: 617 506 118 1033 375 5771 2219
Fragments arranged by size:
5771 2219 1033 617 506 375 118
BsaAI yAC'GTr
Cuts at: 493 1204 2150 2684 8430 493
Size: 711 946 534 5746 2702
Fragments arranged by size:
5746 2702 946 711 534
BsaBI GATnn'nnATC
Cuts at: 3396 3604 3610 3616 3894 4911 10633 3396
Size: 208 6 6 278 1017 5722 3402
Fragments arranged by size:
5722 3402 1017 278 208 6 6
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2554 7739 8446 8828
Size: 53 83 186 936 1021 5185 707 382
Cuts at: 8828 9463 275
Size: 635 1451
Fragments arranged by size:
5185 1451 1021 936 707 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5053 9072 9470 9744 9891 10302 5053
Size: 4019 398 274 147 411 5390
Fragments arranged by size:
5390 4019 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2134 2164 5508 5538 6751
Size: 30 165 30 731 30 3344 30 1213
Cuts at: 6751 6781 7008 7038 7131 7161 1178
Size: 30 227 30 93 30 4656
Fragments arranged by size:
4656 3344 1213 731 227 165 93 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2046 5534 5606 5951 6414 6608 6759 7016
Size: 860 3488 72 345 463 194 151 257
Cuts at: 7016 7364 1186
Size: 348 4461
Fragments arranged by size:
4461 3488 860 463 348 345 257 194
151 72
BseYI C'CCAG_C
Cuts at: 2134 5402 9842 10338 2134
Size: 3268 4440 496 2435
Fragments arranged by size:
4440 3268 2435 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 6822 1163
Size: 573 5086 4980
Fragments arranged by size:
5086 4980 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8089 8850 9878 1540
Size: 275 186 6088 761 1028 2301
Fragments arranged by size:
6088 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2062 2149 8700 8785 9856 2062
Size: 87 6551 85 1071 2845
Fragments arranged by size:
6551 2845 1071 87 85
BsmI GAATG_Cn'
Cuts at: 2544 5406 8150 8243 2544
Size: 2862 2744 93 4940
Fragments arranged by size:
4940 2862 2744 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2062 2149 6270 7185 7958 8700 8785
Size: 399 272 87 4121 915 773 742 85
Cuts at: 8785 9856 1391
Size: 1071 2174
Fragments arranged by size:
4121 2174 1071 915 773 742 399 272
87 85
BspEI T'CCGG_A
Cuts at: 10302 10302
Size: 10639
BspHI T'CATG_A
Cuts at: 2272 6501 8528 10258 2272
Size: 4229 2027 1730 2653
Fragments arranged by size:
4229 2653 2027 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4895 5240 7994 844
Size: 4051 345 2754 3489
Fragments arranged by size:
4051 3489 2754 345
BsrBI CCG'CTC
Cuts at: 1394 1823 3052 8526 1394
Size: 429 1229 5474 3507
Fragments arranged by size:
5474 3507 1229 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4016 6054 7836 9134 9308 65
Size: 1183 2768 2038 1782 1298 174 1396
Fragments arranged by size:
2768 2038 1782 1396 1298 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2310 9283 9470 1700
Size: 110 500 6973 187 2869
Fragments arranged by size:
6973 2869 500 187 110
BsrGI T'GTAC_A
Cuts at: 96 5035 96
Size: 4939 5700
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10639
BssSI C'ACGA_G
Cuts at: 2055 6771 8699 9711 2055
Size: 4716 1928 1012 2983
Fragments arranged by size:
4716 2983 1928 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4181 794
Size: 3387 7252
BstBI TT'CG_AA
Cuts at: 9443 9443
Size: 10639
BstEII G'GTnAC_C
Cuts at: 1184 5216 1184
Size: 4032 6607
BstYI r'GATC_y
Cuts at: 1972 2108 2668 3054 3405 3605 3611 3617 5971
Size: 136 560 386 351 200 6 6 2354
Cuts at: 5971 6414 7021 7516 7969 8336 8721 8738 10190
Size: 443 607 495 453 367 385 17 1452
Cuts at: 10190 10276 10288 10634 1972
Size: 86 12 346 1977
Fragments arranged by size:
2354 1977 1452 607 560 495 453 443
386 385 367 351 346 200 136 86
17 12 6 6
Bsu36I CC'TnA_GG
Cuts at: 6210 7752 7776 6210
Size: 1542 24 9073
Fragments arranged by size:
9073 1542 24
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 10087
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9900
Fragments arranged by size:
9900 683 56
BtsI GCAGTG_nn'
Cuts at: 2355 3850 6412 7240 7840 8155 8949 8969 2355
Size: 1495 2562 828 600 315 794 20 4025
Fragments arranged by size:
4025 2562 1495 828 794 600 315 20
ClaI AT'CG_AT
Cuts at: 1554 2631 5029 1554
Size: 1077 2398 7164
Fragments arranged by size:
7164 2398 1077
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10604
DraI TTT'AAA
Cuts at: 4168 4800 5604 7119 7374 7943 4168
Size: 632 804 1515 255 569 6864
Fragments arranged by size:
6864 1515 804 632 569 255
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3375 4297 9646 809
Size: 507 2059 922 5349 1802
Fragments arranged by size:
5349 2059 1802 922 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2173 7884 8086
Size: 54 1425 50 275 18 343 5711 202
Cuts at: 8086 8975 10604 8
Size: 889 1629 43
Fragments arranged by size:
5711 1629 1425 889 343 275 202 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8086 1537
Size: 275 6274 4090
Fragments arranged by size:
6274 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3212 5430 6208 6816 7068 7266 7308 7430
Size: 1859 2218 778 608 252 198 42 122
Cuts at: 7430 7626 7723 7814 8569 1353
Size: 196 97 91 755 3423
Fragments arranged by size:
3423 2218 1859 778 755 608 252 198
196 122 97 91 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7307 9226 9600 9746 387
Size: 1064 138 5718 1919 374 146 1280
Fragments arranged by size:
5718 1919 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2070 3974 4092 5051 5075 5132 5541
Size: 393 438 1904 118 959 24 57 409
Cuts at: 5541 5760 5814 7032 7290 7707 7731 7941 8702
Size: 219 54 1218 258 417 24 210 761
Cuts at: 8702 9299 10086 10354 1239
Size: 597 787 268 1524
Fragments arranged by size:
1904 1524 1218 959 787 761 597 438
417 409 393 268 258 219 210 118
57 54 24 24
EcoNI CCTnn'n_nnAGG
Cuts at: 4746 7031 4746
Size: 2285 8354
EcoRI G'AATT_C
Cuts at: 2154 5718 5862 7948 8326 2154
Size: 3564 144 2086 378 4467
Fragments arranged by size:
4467 3564 2086 378 144
EcoRV GAT'ATC
Cuts at: 4807 4807
Size: 10639
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2615 2690 4825 5081 5272 6452 7334 2615
Size: 75 2135 256 191 1180 882 5920
Fragments arranged by size:
5920 2135 1180 882 256 191 75
FspI TGC'GCA
Cuts at: 9145 9145
Size: 10639
HaeII r_GCGC'y
Cuts at: 1678 2557 3879 9786 1678
Size: 879 1322 5907 2531
Fragments arranged by size:
5907 2531 1322 879
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2068 2100 2898 2930 2959
Size: 32 135 32 496 32 798 32 29
Cuts at: 2959 2991 3319 3351 3680 3712 3935 3967 5348
Size: 32 328 32 329 32 223 32 1381
Cuts at: 5348 5380 5508 5540 6483 6515 7268 7300 7649
Size: 32 128 32 943 32 753 32 349
Cuts at: 7649 7681 7840 7872 9280 9312 9354 9386 1373
Size: 32 159 32 1408 32 42 32 2626
Fragments arranged by size:
2626 1408 1381 943 798 753 496 349
329 328 223 159 135 128 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 29
HincII GTy'rAC
Cuts at: 669 1657 1938 2938 3368 3380 5229 7983 8229
Size: 988 281 1000 430 12 1849 2754 246
Cuts at: 8229 669
Size: 3079
Fragments arranged by size:
3079 2754 1849 1000 988 430 281 246
12
HindIII A'AGCT_T
Cuts at: 748 3998 4488 6434 748
Size: 3250 490 1946 4953
Fragments arranged by size:
4953 3250 1946 490
HpaI GTT'AAC
Cuts at: 8229 8229
Size: 10639
KasI G'GCGC_C
Cuts at: 2553 2553
Size: 10639
KpnI G_GTAC'C
Cuts at: 7964 7964
Size: 10639
MfeI C'AATT_G
Cuts at: 5325 8238 5325
Size: 2913 7726
MluI A'CGCG_T
Cuts at: 10515 10515
Size: 10639
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2729 3000 3076 3251 5065 7324 7583 9753
Size: 1424 271 76 175 1814 2259 259 2170
Cuts at: 9753 9937 1305
Size: 184 2007
Fragments arranged by size:
2259 2170 2007 1814 1424 271 259 184
175 76
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2175 7886 10
Size: 54 1425 343 343 5711 2763
Fragments arranged by size:
5711 2763 1425 343 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3201 3283 8597 8956 9115 518
Size: 648 2035 82 5314 359 159 2042
Fragments arranged by size:
5314 2042 2035 648 359 159 82
MspA1I CmG'CkG
Cuts at: 1606 6836 8055 8732 9880 10125 1606
Size: 5230 1219 677 1148 245 2120
Fragments arranged by size:
5230 2120 1219 1148 677 245
NarI GG'CG_CC
Cuts at: 2554 2554
Size: 10639
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 10087
NdeI CA'TA_TG
Cuts at: 387 5223 387
Size: 4836 5803
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10639
NotI GC'GGCC_GC
Cuts at: 8086 8086
Size: 10639
NsiI A_TGCA'T
Cuts at: 3543 3543
Size: 10639
NspI r_CATG'y
Cuts at: 1132 1229 2645 3095 3200 3550 5472 7997 9542
Size: 97 1416 450 105 350 1922 2525 1545
Cuts at: 9542 1132
Size: 2229
Fragments arranged by size:
2525 2229 1922 1545 1416 450 350 105
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10639
PciI A'CATG_T
Cuts at: 3091 3546 5468 9538 3091
Size: 455 1922 4070 4192
Fragments arranged by size:
4192 4070 1922 455
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4061 4793 1108
Size: 2953 732 6954
Fragments arranged by size:
6954 2953 732
PfoI T'CCnGG_A
Cuts at: 2086 4249 2086
Size: 2163 8476
PmeI GTTT'AAAC
Cuts at: 7943 7943
Size: 10639
PmlI CAC'GTG
Cuts at: 2150 2150
Size: 10639
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7008 7040 8708 8740 10221 10253 7008
Size: 32 1668 32 1481 32 7394
Fragments arranged by size:
7394 1668 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10639
PsiI TTA'TAA
Cuts at: 4043 4976 8209 4043
Size: 933 3233 6473
Fragments arranged by size:
6473 3233 933
PspXI vC'TCGA_Gb
Cuts at: 2028 2028
Size: 10639
PstI C_TGCA'G
Cuts at: 830 6067 7991 830
Size: 5237 1924 3478
Fragments arranged by size:
5237 3478 1924
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10639
PvuII CAG'CTG
Cuts at: 6836 6836
Size: 10639
SalI G'TCGA_C
Cuts at: 1936 3366 7981 1936
Size: 1430 4615 4594
Fragments arranged by size:
4615 4594 1430
SapI GCTCTTCn'nnn_
Cuts at: 5430 7068 7308 5430
Size: 1638 240 8761
Fragments arranged by size:
8761 1638 240
SbfI CC_TGCA'GG
Cuts at: 7991 7991
Size: 10639
ScaI AGT'ACT
Cuts at: 5398 8887 10599 5398
Size: 3489 1712 5438
Fragments arranged by size:
5438 3489 1712
SfcI C'TryA_G
Cuts at: 826 1045 4407 4428 4758 5787 6063 6222 7209
Size: 219 3362 21 330 1029 276 159 987
Cuts at: 7209 7987 9122 9803 9994 826
Size: 778 1135 681 191 1471
Fragments arranged by size:
3362 1471 1135 1029 987 778 681 330
276 219 191 159 21
SfoI GGC'GCC
Cuts at: 2555 2555
Size: 10639
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10639
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10639
SmaI CCC'GGG
Cuts at: 1872 7966 1872
Size: 6094 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2028 2425 3505 5511 8743 9644
Size: 197 926 85 397 1080 2006 3232 901
Cuts at: 9644 9906 820
Size: 262 1553
Fragments arranged by size:
3232 2006 1553 1080 926 901 397 262
197 85
SnaBI TAC'GTA
Cuts at: 493 2684 8430 493
Size: 2191 5746 2702
Fragments arranged by size:
5746 2702 2191
SpeI A'CTAG_T
Cuts at: 152 6131 6140 152
Size: 5979 9 4651
Fragments arranged by size:
5979 4651 9
SphI G_CATG'C
Cuts at: 1132 2645 7997 1132
Size: 1513 5352 3774
Fragments arranged by size:
5352 3774 1513
SspI AAT'ATT
Cuts at: 5 52 3273 5212 5826 5902 8563 5
Size: 47 3221 1939 614 76 2661 2081
Fragments arranged by size:
3221 2661 2081 1939 614 76 47
StuI AGG'CCT
Cuts at: 1517 2083 2104 7637 1517
Size: 566 21 5533 4519
Fragments arranged by size:
5533 4519 566 21
StyI C'CwwG_G
Cuts at: 513 1065 3482 4398 4980 6306 8078 513
Size: 552 2417 916 582 1326 1772 3074
Fragments arranged by size:
3074 2417 1772 1326 916 582 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2838 3138 3513 8666 8683 8836 9021
Size: 1255 1258 300 375 5153 17 153 185
Cuts at: 9021 9466 325
Size: 445 1498
Fragments arranged by size:
5153 1498 1258 1255 445 375 300 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2395 3748 4067
Size: 274 80 33 51 1443 418 1353 319
Cuts at: 4067 4194 5035 5074 5396 7850 8885 10597 96
Size: 127 841 39 322 2454 1035 1712 138
Fragments arranged by size:
2454 1712 1443 1353 1035 841 418 322
319 274 138 127 80 51 39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2134 2166 2653 2685 4218 4250 6538 6570 10599
Size: 32 487 32 1533 32 2288 32 4029
Cuts at: 10599 10631 2134
Size: 32 2142
Fragments arranged by size:
4029 2288 2142 1533 487 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10639
XbaI T'CTAG_A
Cuts at: 3491 3596 4528 5722 7975 3491
Size: 105 932 1194 2253 6155
Fragments arranged by size:
6155 2253 1194 932 105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2260 6346 2260
Size: 4086 6553
XhoI C'TCGA_G
Cuts at: 1943 2028 1943
Size: 85 10554
XmaI C'CCGG_G
Cuts at: 1870 7964 1870
Size: 6094 4545
XmnI GAAnn'nnTTC
Cuts at: 3463 5535 8768 3463
Size: 2072 3233 5334
Fragments arranged by size:
5334 3233 2072
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8447 276
Size: 53 83 186 936 6913 2468
Fragments arranged by size:
6913 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI BbvCI Bpu10I BsiWI BsmBI BstXI
BstZ17I DraIII EcoICRI EcoO109I FseI FspAI NaeI NgoMIV
NruI PacI PpuMI PspOMI PsrI RsrII SacI SacII
SanDI SexAI SfiI SrfI SwaI