Restriction map for
pFN21AB5995
(Circular) (Six-base) MAPSORT of: pFN21AB5995.seq Check: 4847 from: 1 to: 6436
pFN21AB5995 6436 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4246 278
Size: 53 83 186 936 2710 2468
Fragments arranged by size:
2710 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2148 3544 3779 1937
Size: 211 1396 235 4594
Fragments arranged by size:
4594 1396 235 211
Acc65I G'GTAC_C
Cuts at: 3757 3757
Size: 6436
AclI AA'CG_TT
Cuts at: 1563 4563 4936 1563
Size: 3000 373 3063
Fragments arranged by size:
3063 3000 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2109 2327 2477 4499 5883 6151 1632
Size: 477 218 150 2022 1384 268 1917
Fragments arranged by size:
2022 1917 1384 477 268 218 150
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6239
AflIII A'CryG_T
Cuts at: 1203 2484 5335 6312 1203
Size: 1281 2851 977 1327
Fragments arranged by size:
2851 1327 1281 977
AgeI A'CCGG_T
Cuts at: 5267 5267
Size: 6436
AhdI GACnn_n'nnGTC
Cuts at: 2361 5165 2361
Size: 2804 3632
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6436
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6436
AlwNI CAG_nnn'CTG
Cuts at: 2792 2804 3419 4179 5751 2792
Size: 12 615 760 1572 3477
Fragments arranged by size:
3477 1572 760 615 12
ApaLI G'TGCA_C
Cuts at: 4493 5649 4493
Size: 1156 5280
ApoI r'AATT_y
Cuts at: 1493 2246 2571 3745 3971 4123 6232 1493
Size: 753 325 1174 226 152 2109 1697
Fragments arranged by size:
2109 1697 1174 753 325 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4990 160
Size: 872 3958 1606
Fragments arranged by size:
3958 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6436
AvaI C'yCGr_G
Cuts at: 1870 1943 2958 3137 3225 3255 3345 3578 3761
Size: 73 1015 179 88 30 90 233 183
Cuts at: 3761 1870
Size: 4545
Fragments arranged by size:
4545 1015 233 183 179 90 88 73
30
BamHI G'GATC_C
Cuts at: 2551 2651 2930 3220 3494 3766 4133 2551
Size: 100 279 290 274 272 367 4854
Fragments arranged by size:
4854 367 290 279 274 272 100
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2324 3757 5212 618
Size: 325 217 627 537 1433 1455 1842
Fragments arranged by size:
1842 1455 1433 627 537 325 217
BanII G_rGCy'C
Cuts at: 1391 3144 3755 1391
Size: 1753 611 4072
Fragments arranged by size:
4072 1753 611
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5465
BbvCI CC'TCA_GC
Cuts at: 2619 2619
Size: 6436
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4627 4661 1903
Size: 34 2690 34 3678
Fragments arranged by size:
3678 2690 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3394 3701 4328 5538 3394
Size: 307 627 1210 4292
Fragments arranged by size:
4292 1210 627 307
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6436
BfrBI ATG'CAT
Cuts at: 2716 2716
Size: 6436
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2693 3266 3564
Size: 107 122 71 631 605 1021 573 298
Cuts at: 3564 5047 136
Size: 1483 1525
Fragments arranged by size:
1525 1483 1021 631 605 573 298 122
107 71
BglII A'GATC_T
Cuts at: 6431 6431
Size: 6436
BlpI GC'TnA_GC
Cuts at: 3853 3853
Size: 6436
Bme1580I G_kGCm'C
Cuts at: 1790 2329 4497 5653 1790
Size: 539 2168 1156 2573
Fragments arranged by size:
2573 2168 1156 539
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6436
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2692 3407 3453
Size: 416 53 83 399 24 1275 715 46
Cuts at: 3453 3503 4167 5125 442
Size: 50 664 958 1753
Fragments arranged by size:
1753 1275 958 715 664 416 399 83
53 50 46 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2847 3039 3747 5096 1239
Size: 1608 192 708 1349 2579
Fragments arranged by size:
2579 1608 1349 708 192
Bpu10I CC'TnA_GC
Cuts at: 2619 2647 2818 2844 3675 2619
Size: 28 171 26 831 5380
Fragments arranged by size:
5380 831 171 28 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4561 5426 5724 4561
Size: 865 298 5273
Fragments arranged by size:
5273 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2019 2348 5099 882
Size: 617 520 329 2751 2219
Fragments arranged by size:
2751 2219 617 520 329
BsaAI yAC'GTr
Cuts at: 493 1204 4227 493
Size: 711 3023 2702
Fragments arranged by size:
3023 2702 711
BsaBI GATnn'nnATC
Cuts at: 6430 6430
Size: 6436
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4243 4625 5260 275
Size: 53 83 186 936 2710 382 635 1451
Fragments arranged by size:
2710 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2554 4869 5267 5541 5688 6099 2554
Size: 2315 398 274 147 411 2891
Fragments arranged by size:
2891 2315 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3720 3750 1178
Size: 30 165 30 2317 30 3864
Fragments arranged by size:
3864 2317 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2724 2844 1186
Size: 1538 120 4778
Fragments arranged by size:
4778 1538 120
BseYI C'CCAG_C
Cuts at: 2029 2636 5639 6135 2029
Size: 607 3003 496 2330
Fragments arranged by size:
3003 2330 607 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5863
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3886 4647 5675 1540
Size: 275 186 1885 761 1028 2301
Fragments arranged by size:
2301 1885 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2263 3733 4497 4582 5653 2263
Size: 1470 764 85 1071 3046
Fragments arranged by size:
3046 1470 1071 764 85
BsmI GAATG_Cn'
Cuts at: 2718 2841 2859 3453 3534 3947 4040 2718
Size: 123 18 594 81 413 93 5114
Fragments arranged by size:
5114 594 413 123 93 81 18
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2263 2329 3144 3733 3755 4497 4582
Size: 399 473 66 815 589 22 742 85
Cuts at: 4582 5653 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 815 742 589 473 399 85
66 22
BspEI T'CCGG_A
Cuts at: 2554 6099 2554
Size: 3545 2891
BspHI T'CATG_A
Cuts at: 3607 4325 6055 3607
Size: 718 1730 3988
Fragments arranged by size:
3988 1730 718
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3622 3791 844
Size: 2778 169 3489
Fragments arranged by size:
3489 2778 169
BsrBI CCG'CTC
Cuts at: 1394 1823 4323 1394
Size: 429 2500 3507
Fragments arranged by size:
3507 2500 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3312 3342 3372 4931 5105 65
Size: 1183 2064 30 30 1559 174 1396
Fragments arranged by size:
2064 1559 1396 1183 174 30 30
BsrFI r'CCGG_y
Cuts at: 1700 1810 5080 5267 1700
Size: 110 3270 187 2869
Fragments arranged by size:
3270 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6436
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6436
BssSI C'ACGA_G
Cuts at: 3240 3330 4496 5508 3240
Size: 90 1166 1012 4168
Fragments arranged by size:
4168 1166 1012 90
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2219 794
Size: 1425 5011
BstBI TT'CG_AA
Cuts at: 5240 5240
Size: 6436
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6436
BstXI CCAn_nnnn'nTGG
Cuts at: 2636 3083 3397 2636
Size: 447 314 5675
Fragments arranged by size:
5675 447 314
BstYI r'GATC_y
Cuts at: 1972 2551 2651 2930 3220 3494 3766 4133 4518
Size: 579 100 279 290 274 272 367 385
Cuts at: 4518 4535 5987 6073 6085 6431 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 579 385 367 346 290 279
274 272 100 86 17 12
BstZ17I GTA'TAC
Cuts at: 2149 3545 2149
Size: 1396 5040
Bsu36I CC'TnA_GG
Cuts at: 2367 2682 2691 2367
Size: 315 9 6112
Fragments arranged by size:
6112 315 9
BtgI C'CryG_G
Cuts at: 513 1065 2004 2629 2877 2990 3261 513
Size: 552 939 625 248 113 271 3688
Fragments arranged by size:
3688 939 625 552 271 248 113
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2564 3335 3365 487
Size: 683 56 1338 771 30 3558
Fragments arranged by size:
3558 1338 771 683 56 30
BtsI GCAGTG_nn'
Cuts at: 3952 4746 4766 3952
Size: 794 20 5622
Fragments arranged by size:
5622 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6436
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6401
DraI TTT'AAA
Cuts at: 3702 3740 3702
Size: 38 6398
DraIII CAC_nnn'GTG
Cuts at: 2219 2219
Size: 6436
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5443 809
Size: 507 4127 1802
Fragments arranged by size:
4127 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3085 3883 4772
Size: 54 1425 50 275 18 1255 798 889
Cuts at: 4772 6401 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1255 889 798 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3883 1537
Size: 275 2071 4090
Fragments arranged by size:
4090 2071 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2085 2295 2303 4366 1353
Size: 732 210 8 2063 3423
Fragments arranged by size:
3423 2063 732 210 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5023 5397 5543 387
Size: 1064 138 3434 374 146 1280
Fragments arranged by size:
3434 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2109 2327 2477 2847 3039 3747 4499
Size: 393 477 218 150 370 192 708 752
Cuts at: 4499 5096 5883 6151 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 752 708 597 477 393 370
268 218 192 150
EcoNI CCTnn'n_nnAGG
Cuts at: 2646 2829 2646
Size: 183 6253
EcoO109I rG'GnC_Cy
Cuts at: 2686 2801 3170 3431 3455 3505 2686
Size: 115 369 261 24 50 5617
Fragments arranged by size:
5617 369 261 115 50 24
EcoRI G'AATT_C
Cuts at: 2246 3745 4123 2246
Size: 1499 378 4559
Fragments arranged by size:
4559 1499 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2125 2125
Size: 6436
FspI TGC'GCA
Cuts at: 4942 4942
Size: 6436
HaeII r_GCGC'y
Cuts at: 1678 5583 1678
Size: 3905 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2343 2347 2375 2379 3313
Size: 32 135 32 771 4 28 4 934
Cuts at: 3313 3345 5077 5109 5151 5183 1373
Size: 32 1732 32 42 32 2626
Fragments arranged by size:
2626 1732 934 771 135 42 32 32
32 32 32 28 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3780 4026 669
Size: 988 281 1842 246 3079
Fragments arranged by size:
3079 1842 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2745 748
Size: 1997 4439
HpaI GTT'AAC
Cuts at: 4026 4026
Size: 6436
KpnI G_GTAC'C
Cuts at: 3761 3761
Size: 6436
MfeI C'AATT_G
Cuts at: 4035 4035
Size: 6436
MluI A'CGCG_T
Cuts at: 6312 6312
Size: 6436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2101 2830 5550 5734 1305
Size: 796 729 2720 184 2007
Fragments arranged by size:
2720 2007 796 729 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3087 10
Size: 54 1425 343 1255 3359
Fragments arranged by size:
3359 1425 1255 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2721 4394 4753 4912 518
Size: 648 1555 1673 359 159 2042
Fragments arranged by size:
2042 1673 1555 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2033 2789 2926 3852 4529 5677 5922 1606
Size: 427 756 137 926 677 1148 245 2120
Fragments arranged by size:
2120 1148 926 756 677 427 245 137
NcoI C'CATG_G
Cuts at: 513 1065 2004 2629 2877 3261 513
Size: 552 939 625 248 384 3688
Fragments arranged by size:
3688 939 625 552 384 248
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6436
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6436
NotI GC'GGCC_GC
Cuts at: 3883 3883
Size: 6436
NsiI A_TGCA'T
Cuts at: 2718 2718
Size: 6436
NspI r_CATG'y
Cuts at: 1132 1229 2488 2720 3794 5339 1132
Size: 97 1259 232 1074 1545 2229
Fragments arranged by size:
2229 1545 1259 1074 232 97
PasI CC'CwG_GG
Cuts at: 1348 3490 1348
Size: 2142 4294
PciI A'CATG_T
Cuts at: 2484 5335 2484
Size: 2851 3585
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2630 2704 2864 3419 1108
Size: 1522 74 160 555 4125
Fragments arranged by size:
4125 1522 555 160 74
PfoI T'CCnGG_A
Cuts at: 2824 2824
Size: 6436
PmeI GTTT'AAAC
Cuts at: 3740 3740
Size: 6436
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4505 4537 6018 6050 4505
Size: 32 1481 32 4891
Fragments arranged by size:
4891 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2801 3170 3431 3455 3505 2801
Size: 369 261 24 50 5732
Fragments arranged by size:
5732 369 261 50 24
PshAI GACnn'nnGTC
Cuts at: 1613 2872 1613
Size: 1259 5177
PsiI TTA'TAA
Cuts at: 4006 4006
Size: 6436
PspXI vC'TCGA_Gb
Cuts at: 2958 2958
Size: 6436
PstI C_TGCA'G
Cuts at: 830 3652 3788 830
Size: 2822 136 3478
Fragments arranged by size:
3478 2822 136
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6436
PvuII CAG'CTG
Cuts at: 2789 2789
Size: 6436
SalI G'TCGA_C
Cuts at: 1936 3778 1936
Size: 1842 4594
SanDI GG'GwC_CC
Cuts at: 3455 3455
Size: 6436
SapI GCTCTTCn'nnn_
Cuts at: 2085 2295 2303 2085
Size: 210 8 6218
Fragments arranged by size:
6218 210 8
SbfI CC_TGCA'GG
Cuts at: 3788 3788
Size: 6436
ScaI AGT'ACT
Cuts at: 4684 6396 4684
Size: 1712 4724
SfcI C'TryA_G
Cuts at: 826 1045 3648 3784 4919 5600 5791 826
Size: 219 2603 136 1135 681 191 1471
Fragments arranged by size:
2603 1471 1135 681 219 191 136
SfiI GGCCn_nnn'nGGCC
Cuts at: 2693 2693
Size: 6436
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6436
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6436
SmaI CCC'GGG
Cuts at: 1872 3580 3763 1872
Size: 1708 183 4545
Fragments arranged by size:
4545 1708 183
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2958 4540 5441 5703 820
Size: 197 926 1015 1582 901 262 1553
Fragments arranged by size:
1582 1553 1015 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4227 493
Size: 3734 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6436
SphI G_CATG'C
Cuts at: 1132 3794 1132
Size: 2662 3774
SspI AAT'ATT
Cuts at: 5 52 4360 5
Size: 47 4308 2081
Fragments arranged by size:
4308 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6436
StyI C'CwwG_G
Cuts at: 513 1065 2004 2314 2629 2877 3198 3261 3875
Size: 552 939 310 315 248 321 63 614
Cuts at: 3875 513
Size: 3074
Fragments arranged by size:
3074 939 614 552 321 315 310 248
63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2696 3082 4463 4480 4633 4818 5263
Size: 1255 1116 386 1381 17 153 185 445
Cuts at: 5263 325
Size: 1498
Fragments arranged by size:
1498 1381 1255 1116 445 386 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4682 6394 96
Size: 274 80 33 51 1443 2705 1712 138
Fragments arranged by size:
2705 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2643 2675 6396 6428 2643
Size: 32 3721 32 2651
Fragments arranged by size:
3721 2651 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6436
XbaI T'CTAG_A
Cuts at: 3772 3772
Size: 6436
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2705 3151 2705
Size: 446 5990
XhoI C'TCGA_G
Cuts at: 1943 2958 1943
Size: 1015 5421
XmaI C'CCGG_G
Cuts at: 1870 3578 3761 1870
Size: 1708 183 4545
Fragments arranged by size:
4545 1708 183
XmnI GAAnn'nnTTC
Cuts at: 4565 4565
Size: 6436
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4244 276
Size: 53 83 186 936 2710 2468
Fragments arranged by size:
2710 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI EcoICRI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspOMI PsrI
RsrII SacI SacII SexAI SfoI SrfI SwaI