Restriction map for
pFN21AB5994
(Circular) (Six-base) MAPSORT of: pFN21AB5994.seq Check: 9052 from: 1 to: 6532
pFN21AB5994 6532 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4342 278
Size: 53 83 186 936 2806 2468
Fragments arranged by size:
2806 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3875 1937
Size: 1938 4594
Acc65I G'GTAC_C
Cuts at: 3853 3853
Size: 6532
AclI AA'CG_TT
Cuts at: 1563 3593 4659 5032 1563
Size: 2030 1066 373 3063
Fragments arranged by size:
3063 2030 1066 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2153 2921 3420 4595 5979 6247 1632
Size: 521 768 499 1175 1384 268 1917
Fragments arranged by size:
1917 1384 1175 768 521 499 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6335
AflIII A'CryG_T
Cuts at: 1203 2231 5431 6408 1203
Size: 1028 3200 977 1327
Fragments arranged by size:
3200 1327 1028 977
AgeI A'CCGG_T
Cuts at: 5363 5363
Size: 6532
AhdI GACnn_n'nnGTC
Cuts at: 5261 5261
Size: 6532
AleI CACnn'nnGTG
Cuts at: 1166 2088 1166
Size: 922 5610
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3631 1184
Size: 2447 4085
AlwNI CAG_nnn'CTG
Cuts at: 4275 5847 4275
Size: 1572 4960
ApaLI G'TGCA_C
Cuts at: 2446 4589 5745 2446
Size: 2143 1156 3233
Fragments arranged by size:
3233 2143 1156
ApoI r'AATT_y
Cuts at: 1493 2128 2219 2690 3203 3232 3281 3841 4067
Size: 635 91 471 513 29 49 560 226
Cuts at: 4067 4219 6328 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 635 560 513 471 226 152
91 49 29
AseI AT'TA_AT
Cuts at: 160 1032 5086 160
Size: 872 4054 1606
Fragments arranged by size:
4054 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6532
AvaI C'yCGr_G
Cuts at: 1870 1943 3857 1870
Size: 73 1914 4545
Fragments arranged by size:
4545 1914 73
AvrII C'CTAG_G
Cuts at: 3364 3364
Size: 6532
BamHI G'GATC_C
Cuts at: 3862 4229 3862
Size: 367 6165
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3853 5308 618
Size: 325 217 627 2066 1455 1842
Fragments arranged by size:
2066 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3851 1391
Size: 2460 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2515 3043 3432 928
Size: 971 616 528 389 4028
Fragments arranged by size:
4028 971 616 528 389
BbvCI CC'TCA_GC
Cuts at: 3036 3036
Size: 6532
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4723 4757 1903
Size: 34 2786 34 3678
Fragments arranged by size:
3678 2786 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4424 5634 4424
Size: 1210 5322
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6532
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5143 136
Size: 107 122 71 631 605 3471 1525
Fragments arranged by size:
3471 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2785 2821 6527 2785
Size: 36 3706 2790
Fragments arranged by size:
3706 2790 36
BlpI GC'TnA_GC
Cuts at: 3949 3949
Size: 6532
Bme1580I G_kGCm'C
Cuts at: 1790 2450 4593 5749 1790
Size: 660 2143 1156 2573
Fragments arranged by size:
2573 2143 1156 660
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6532
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4263 5221 442
Size: 416 53 83 399 24 2846 958 1753
Fragments arranged by size:
2846 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6532
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2875 5192 1239
Size: 1636 2317 2579
Fragments arranged by size:
2579 2317 1636
Bpu10I CC'TnA_GC
Cuts at: 2732 3036 2732
Size: 304 6228
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2654 3340 4657 5522 5820 2654
Size: 686 1317 865 298 3366
Fragments arranged by size:
3366 1317 865 686 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2419 3358 3764 5195 882
Size: 617 920 939 406 1431 2219
Fragments arranged by size:
2219 1431 939 920 617 406
BsaAI yAC'GTr
Cuts at: 493 1204 4323 493
Size: 711 3119 2702
Fragments arranged by size:
3119 2702 711
BsaBI GATnn'nnATC
Cuts at: 6526 6526
Size: 6532
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4339 4721 5356 275
Size: 53 83 186 936 2806 382 635 1451
Fragments arranged by size:
2806 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4965 5363 5637 5784 6195 4965
Size: 398 274 147 411 5302
Fragments arranged by size:
5302 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6307
Fragments arranged by size:
6307 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3800 1186
Size: 2614 3918
BseYI C'CCAG_C
Cuts at: 5735 6231 5735
Size: 496 6036
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3441 3490 3642 1163
Size: 573 1705 49 152 4053
Fragments arranged by size:
4053 1705 573 152 49
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3982 4743 5771 1540
Size: 275 186 1981 761 1028 2301
Fragments arranged by size:
2301 1981 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2450 4593 4678 5749 2450
Size: 2143 85 1071 3233
Fragments arranged by size:
3233 2143 1071 85
BsmI GAATG_Cn'
Cuts at: 2381 2813 2912 3185 3295 3417 4043 4136 2381
Size: 432 99 273 110 122 626 93 4777
Fragments arranged by size:
4777 626 432 273 122 110 99 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2450 3851 4593 4678 5749 1391
Size: 399 660 1401 742 85 1071 2174
Fragments arranged by size:
2174 1401 1071 742 660 399 85
BspEI T'CCGG_A
Cuts at: 6195 6195
Size: 6532
BspHI T'CATG_A
Cuts at: 2458 3175 4421 6151 2458
Size: 717 1246 1730 2839
Fragments arranged by size:
2839 1730 1246 717
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3782 3887 844
Size: 2938 105 3489
Fragments arranged by size:
3489 2938 105
BsrBI CCG'CTC
Cuts at: 1394 1823 4419 1394
Size: 429 2596 3507
Fragments arranged by size:
3507 2596 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2943 5027 5201 65
Size: 1183 1695 2084 174 1396
Fragments arranged by size:
2084 1695 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5176 5363 1700
Size: 110 3366 187 2869
Fragments arranged by size:
3366 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6532
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6532
BssSI C'ACGA_G
Cuts at: 4592 5604 4592
Size: 1012 5520
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6532
BstBI TT'CG_AA
Cuts at: 5336 5336
Size: 6532
BstEII G'GTnAC_C
Cuts at: 1184 2014 1184
Size: 830 5702
BstXI CCAn_nnnn'nTGG
Cuts at: 2693 2693
Size: 6532
BstYI r'GATC_y
Cuts at: 1972 2785 2821 3169 3862 4229 4614 4631 6083
Size: 813 36 348 693 367 385 17 1452
Cuts at: 6083 6169 6181 6527 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 813 693 385 367 348 346
86 36 17 12
Bsu36I CC'TnA_GG
Cuts at: 3823 3823
Size: 6532
BtgI C'CryG_G
Cuts at: 513 1065 2815 3438 513
Size: 552 1750 623 3607
Fragments arranged by size:
3607 1750 623 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5793
Fragments arranged by size:
5793 683 56
BtsI GCAGTG_nn'
Cuts at: 2180 2448 2938 4048 4842 4862 2180
Size: 268 490 1110 794 20 3850
Fragments arranged by size:
3850 1110 794 490 268 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6532
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6497
DraI TTT'AAA
Cuts at: 2110 3208 3836 2110
Size: 1098 628 4806
Fragments arranged by size:
4806 1098 628
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5539 809
Size: 507 4223 1802
Fragments arranged by size:
4223 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3979 4868 6497
Size: 54 1425 50 275 18 2149 889 1629
Cuts at: 6497 8
Size: 43
Fragments arranged by size:
2149 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3979 1537
Size: 275 2167 4090
Fragments arranged by size:
4090 2167 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2319 2463 3216 4462 1353
Size: 966 144 753 1246 3423
Fragments arranged by size:
3423 1246 966 753 144
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2864 5119 5493 5639 387
Size: 1064 138 1275 2255 374 146 1280
Fragments arranged by size:
2255 1280 1275 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2875 2921 3420 4595 5192 5979
Size: 393 521 722 46 499 1175 597 787
Cuts at: 5979 6247 1239
Size: 268 1524
Fragments arranged by size:
1524 1175 787 722 597 521 499 393
268 46
EcoRI G'AATT_C
Cuts at: 3841 4219 3841
Size: 378 6154
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2532 3067 2532
Size: 535 5997
FspI TGC'GCA
Cuts at: 5038 5038
Size: 6532
HaeII r_GCGC'y
Cuts at: 1678 5679 1678
Size: 4001 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2873 2905 3419 3451 5173
Size: 32 135 32 1301 32 514 32 1722
Cuts at: 5173 5205 5247 5279 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1722 1301 514 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3333 3876 4122 669
Size: 988 281 1395 543 246 3079
Fragments arranged by size:
3079 1395 988 543 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6532
HpaI GTT'AAC
Cuts at: 4122 4122
Size: 6532
KpnI G_GTAC'C
Cuts at: 3857 3857
Size: 6532
MfeI C'AATT_G
Cuts at: 4131 4131
Size: 6532
MluI A'CGCG_T
Cuts at: 6408 6408
Size: 6532
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2907 3362 5646 5830 1305
Size: 1602 455 2284 184 2007
Fragments arranged by size:
2284 2007 1602 455 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4710
Fragments arranged by size:
4710 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2088 2394 2463 4490 4849 5008 518
Size: 648 922 306 69 2027 359 159 2042
Fragments arranged by size:
2042 2027 922 648 359 306 159 69
MspA1I CmG'CkG
Cuts at: 1606 2252 3164 3948 4625 5773 6018 1606
Size: 646 912 784 677 1148 245 2120
Fragments arranged by size:
2120 1148 912 784 677 646 245
NcoI C'CATG_G
Cuts at: 513 1065 2815 513
Size: 552 1750 4230
Fragments arranged by size:
4230 1750 552
NdeI CA'TA_TG
Cuts at: 387 3133 387
Size: 2746 3786
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6532
NotI GC'GGCC_GC
Cuts at: 3979 3979
Size: 6532
NspI r_CATG'y
Cuts at: 1132 1229 2235 3064 3890 5435 1132
Size: 97 1006 829 826 1545 2229
Fragments arranged by size:
2229 1545 1006 829 826 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6532
PciI A'CATG_T
Cuts at: 2231 5431 2231
Size: 3200 3332
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 5630
PmeI GTTT'AAAC
Cuts at: 3836 3836
Size: 6532
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4601 4633 6114 6146 4601
Size: 32 1481 32 4987
Fragments arranged by size:
4987 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6532
PsiI TTA'TAA
Cuts at: 4102 4102
Size: 6532
PstI C_TGCA'G
Cuts at: 830 3169 3884 830
Size: 2339 715 3478
Fragments arranged by size:
3478 2339 715
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6532
PvuII CAG'CTG
Cuts at: 2252 2252
Size: 6532
SalI G'TCGA_C
Cuts at: 1936 3874 1936
Size: 1938 4594
SapI GCTCTTCn'nnn_
Cuts at: 3216 3216
Size: 6532
SbfI CC_TGCA'GG
Cuts at: 3884 3884
Size: 6532
ScaI AGT'ACT
Cuts at: 4780 6492 4780
Size: 1712 4820
SfcI C'TryA_G
Cuts at: 826 1045 3165 3880 5015 5696 5887 826
Size: 219 2120 715 1135 681 191 1471
Fragments arranged by size:
2120 1471 1135 715 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6532
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6532
SmaI CCC'GGG
Cuts at: 1872 3859 1872
Size: 1987 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2669 3355 4636 5537 5799 820
Size: 197 926 726 686 1281 901 262 1553
Fragments arranged by size:
1553 1281 926 901 726 686 262 197
SnaBI TAC'GTA
Cuts at: 493 4323 493
Size: 3830 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6532
SphI G_CATG'C
Cuts at: 1132 3890 1132
Size: 2758 3774
SspI AAT'ATT
Cuts at: 5 52 4456 5
Size: 47 4404 2081
Fragments arranged by size:
4404 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6532
StyI C'CwwG_G
Cuts at: 513 1065 2139 2815 3364 3516 3687 3971 513
Size: 552 1074 676 549 152 171 284 3074
Fragments arranged by size:
3074 1074 676 552 549 284 171 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4559 4576 4729 4914 5359 325
Size: 1255 2979 17 153 185 445 1498
Fragments arranged by size:
2979 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3276 4778 6490
Size: 274 80 33 51 1443 1299 1502 1712
Cuts at: 6490 96
Size: 138
Fragments arranged by size:
1712 1502 1443 1299 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6492 6524 6492
Size: 32 6500
Tth111I GACn'n_nGTC
Cuts at: 1316 3491 1316
Size: 2175 4357
XbaI T'CTAG_A
Cuts at: 3145 3868 3145
Size: 723 5809
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6532
XmaI C'CCGG_G
Cuts at: 1870 3857 1870
Size: 1987 4545
XmnI GAAnn'nnTTC
Cuts at: 4661 4661
Size: 6532
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4340 276
Size: 53 83 186 936 2806 2468
Fragments arranged by size:
2806 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI