Restriction map for
pFN21AB5990
(Circular) (Six-base) MAPSORT of: pFN21AB5990.seq Check: 2969 from: 1 to: 6532
pFN21AB5990 6532 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2016 3215 2016
Size: 1199 5333
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2746 4342 278
Size: 53 83 186 936 1210 1596 2468
Fragments arranged by size:
2468 1596 1210 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2384 3875 1937
Size: 447 1491 4594
Fragments arranged by size:
4594 1491 447
Acc65I G'GTAC_C
Cuts at: 3853 3853
Size: 6532
AclI AA'CG_TT
Cuts at: 1563 2676 3439 4659 5032 1563
Size: 1113 763 1220 373 3063
Fragments arranged by size:
3063 1220 1113 763 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4595 5979 6247 1632
Size: 2963 1384 268 1917
Fragments arranged by size:
2963 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6335
AflIII A'CryG_T
Cuts at: 1203 5431 6408 1203
Size: 4228 977 1327
Fragments arranged by size:
4228 1327 977
AgeI A'CCGG_T
Cuts at: 5363 5363
Size: 6532
AhdI GACnn_n'nnGTC
Cuts at: 5261 5261
Size: 6532
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6532
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6532
AlwNI CAG_nnn'CTG
Cuts at: 2764 4275 5847 2764
Size: 1511 1572 3449
Fragments arranged by size:
3449 1572 1511
ApaLI G'TGCA_C
Cuts at: 4589 5745 4589
Size: 1156 5376
ApoI r'AATT_y
Cuts at: 1493 2480 3841 4067 4219 6328 1493
Size: 987 1361 226 152 2109 1697
Fragments arranged by size:
2109 1697 1361 987 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5086 160
Size: 872 4054 1606
Fragments arranged by size:
4054 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6532
AvaI C'yCGr_G
Cuts at: 1870 1943 3327 3857 1870
Size: 73 1384 530 4545
Fragments arranged by size:
4545 1384 530 73
AvrII C'CTAG_G
Cuts at: 3502 3502
Size: 6532
BamHI G'GATC_C
Cuts at: 3128 3862 4229 3128
Size: 734 367 5431
Fragments arranged by size:
5431 734 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2228 3853 5308 618
Size: 325 217 627 441 1625 1455 1842
Fragments arranged by size:
1842 1625 1455 627 441 325 217
BanII G_rGCy'C
Cuts at: 1391 3851 1391
Size: 2460 4072
BbeI G_GCGC'C
Cuts at: 2232 2232
Size: 6532
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3358 3716 928
Size: 971 1459 358 3744
Fragments arranged by size:
3744 1459 971 358
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2756 2790 4723 4757 1903
Size: 34 819 34 1933 34 3678
Fragments arranged by size:
3678 1933 819 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4424 5634 4424
Size: 1210 5322
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6532
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5143 136
Size: 107 122 71 631 605 3471 1525
Fragments arranged by size:
3471 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6527 6527
Size: 6532
BlpI GC'TnA_GC
Cuts at: 3566 3949 3566
Size: 383 6149
Bme1580I G_kGCm'C
Cuts at: 1790 4593 5749 1790
Size: 2803 1156 2573
Fragments arranged by size:
2803 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6532
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4263 5221 442
Size: 416 53 83 399 24 2846 958 1753
Fragments arranged by size:
2846 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6532
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2432 3594 5192 1239
Size: 1193 1162 1598 2579
Fragments arranged by size:
2579 1598 1193 1162
Bpu10I CC'TnA_GC
Cuts at: 2561 2561
Size: 6532
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4657 5522 5820 4657
Size: 865 298 5369
Fragments arranged by size:
5369 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2082 2923 3612 5195 882
Size: 617 583 841 689 1583 2219
Fragments arranged by size:
2219 1583 841 689 617 583
BsaAI yAC'GTr
Cuts at: 493 1204 4323 493
Size: 711 3119 2702
Fragments arranged by size:
3119 2702 711
BsaBI GATnn'nnATC
Cuts at: 6526 6526
Size: 6532
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2229 2743 4339 4721
Size: 53 83 186 936 696 514 1596 382
Cuts at: 4721 5356 275
Size: 635 1451
Fragments arranged by size:
1596 1451 936 696 635 514 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4965 5363 5637 5784 6195 4965
Size: 398 274 147 411 5302
Fragments arranged by size:
5302 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3066 3096 3305 3335 1178
Size: 30 165 30 1663 30 209 30 4375
Fragments arranged by size:
4375 1663 209 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2435 3411 1186
Size: 1249 976 4307
Fragments arranged by size:
4307 1249 976
BseYI C'CCAG_C
Cuts at: 5735 6231 5735
Size: 496 6036
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2062 3004 3531 1163
Size: 573 326 942 527 4164
Fragments arranged by size:
4164 942 573 527 326
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3982 4743 5771 1540
Size: 275 186 1981 761 1028 2301
Fragments arranged by size:
2301 1981 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4593 4678 5749 4593
Size: 85 1071 5376
Fragments arranged by size:
5376 1071 85
BsmI GAATG_Cn'
Cuts at: 2520 2855 4043 4136 2520
Size: 335 1188 93 4916
Fragments arranged by size:
4916 1188 335 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3851 4593 4678 5749 1391
Size: 399 2061 742 85 1071 2174
Fragments arranged by size:
2174 2061 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6195 6195
Size: 6532
BspHI T'CATG_A
Cuts at: 4421 6151 4421
Size: 1730 4802
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2016 2131 3215 3887 844
Size: 1172 115 1084 672 3489
Fragments arranged by size:
3489 1172 1084 672 115
BsrBI CCG'CTC
Cuts at: 1394 1823 4419 1394
Size: 429 2596 3507
Fragments arranged by size:
3507 2596 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2419 5027 5201 65
Size: 1183 1171 2608 174 1396
Fragments arranged by size:
2608 1396 1183 1171 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5176 5363 1700
Size: 110 3366 187 2869
Fragments arranged by size:
3366 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6532
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6532
BssSI C'ACGA_G
Cuts at: 4592 5604 4592
Size: 1012 5520
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6532
BstBI TT'CG_AA
Cuts at: 5336 5336
Size: 6532
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6532
BstXI CCAn_nnnn'nTGG
Cuts at: 2803 2803
Size: 6532
BstYI r'GATC_y
Cuts at: 1972 2500 3128 3862 4229 4614 4631 6083 6169
Size: 528 628 734 367 385 17 1452 86
Cuts at: 6169 6181 6527 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 734 628 528 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2385 2385
Size: 6532
Bsu36I CC'TnA_GG
Cuts at: 3450 3450
Size: 6532
BtgI C'CryG_G
Cuts at: 513 1065 2004 2933 513
Size: 552 939 929 4112
Fragments arranged by size:
4112 939 929 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5793
Fragments arranged by size:
5793 683 56
BtsI GCAGTG_nn'
Cuts at: 2689 2712 2992 3190 3577 3660 4048 4842 4862
Size: 23 280 198 387 83 388 794 20
Cuts at: 4862 2689
Size: 4359
Fragments arranged by size:
4359 794 388 387 280 198 83 23
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6532
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6497
DraI TTT'AAA
Cuts at: 3836 3836
Size: 6532
DraIII CAC_nnn'GTG
Cuts at: 3815 3815
Size: 6532
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5539 809
Size: 507 4223 1802
Fragments arranged by size:
4223 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3262 3618 3979
Size: 54 1425 50 275 18 1432 356 361
Cuts at: 3979 4868 6497 8
Size: 889 1629 43
Fragments arranged by size:
1629 1432 1425 889 361 356 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3979 1537
Size: 275 2167 4090
Fragments arranged by size:
4090 2167 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2400 2430 2610 4462 1353
Size: 1047 30 180 1852 3423
Fragments arranged by size:
3423 1852 1047 180 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5119 5493 5639 387
Size: 1064 138 3530 374 146 1280
Fragments arranged by size:
3530 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2432 3594 4595 5192 5979 6247 1239
Size: 393 800 1162 1001 597 787 268 1524
Fragments arranged by size:
1524 1162 1001 800 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3486 3486
Size: 6532
EcoRI G'AATT_C
Cuts at: 2480 3841 4219 2480
Size: 1361 378 4793
Fragments arranged by size:
4793 1361 378
FspI TGC'GCA
Cuts at: 2024 5038 2024
Size: 3014 3518
HaeII r_GCGC'y
Cuts at: 1678 2232 5679 1678
Size: 554 3447 2531
Fragments arranged by size:
3447 2531 554
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2642 2674 3066 3098 3116
Size: 32 135 32 1070 32 392 32 18
Cuts at: 3116 3148 3260 3289 3292 3321 3401 3406 3433
Size: 32 112 29 3 29 80 5 27
Cuts at: 3433 3438 5173 5205 5247 5279 1373
Size: 5 1735 32 42 32 2626
Fragments arranged by size:
2626 1735 1070 392 135 112 80 42
32 32 32 32 32 32 32 29
29 27 18 5 5 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3876 4122 669
Size: 988 281 1938 246 3079
Fragments arranged by size:
3079 1938 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6532
HpaI GTT'AAC
Cuts at: 4122 4122
Size: 6532
KasI G'GCGC_C
Cuts at: 2228 2228
Size: 6532
KpnI G_GTAC'C
Cuts at: 3857 3857
Size: 6532
MfeI C'AATT_G
Cuts at: 4131 4131
Size: 6532
MluI A'CGCG_T
Cuts at: 6408 6408
Size: 6532
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5646 5830 1305
Size: 4341 184 2007
Fragments arranged by size:
4341 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3264 3620 10
Size: 54 1425 343 1432 356 2922
Fragments arranged by size:
2922 1432 1425 356 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2545 4490 4849 5008 518
Size: 648 1379 1945 359 159 2042
Fragments arranged by size:
2042 1945 1379 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2076 2435 2452 3948 4625 5773 6018 1606
Size: 470 359 17 1496 677 1148 245 2120
Fragments arranged by size:
2120 1496 1148 677 470 359 245 17
NarI GG'CG_CC
Cuts at: 2229 2229
Size: 6532
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5041
Fragments arranged by size:
5041 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6532
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6532
NotI GC'GGCC_GC
Cuts at: 3979 3979
Size: 6532
NspI r_CATG'y
Cuts at: 1132 1229 2134 2774 3890 5435 1132
Size: 97 905 640 1116 1545 2229
Fragments arranged by size:
2229 1545 1116 905 640 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6532
PciI A'CATG_T
Cuts at: 5431 5431
Size: 6532
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3036 3603 3744 1108
Size: 1928 567 141 3896
Fragments arranged by size:
3896 1928 567 141
PmeI GTTT'AAAC
Cuts at: 3836 3836
Size: 6532
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4601 4633 6114 6146 4601
Size: 32 1481 32 4987
Fragments arranged by size:
4987 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3486 3486
Size: 6532
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6532
PsiI TTA'TAA
Cuts at: 4102 4102
Size: 6532
PstI C_TGCA'G
Cuts at: 830 2769 3212 3884 830
Size: 1939 443 672 3478
Fragments arranged by size:
3478 1939 672 443
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6532
PvuII CAG'CTG
Cuts at: 2435 2452 2435
Size: 17 6515
SalI G'TCGA_C
Cuts at: 1936 3874 1936
Size: 1938 4594
SanDI GG'GwC_CC
Cuts at: 3486 3486
Size: 6532
SbfI CC_TGCA'GG
Cuts at: 3212 3884 3212
Size: 672 5860
ScaI AGT'ACT
Cuts at: 2961 3234 4780 6492 2961
Size: 273 1546 1712 3001
Fragments arranged by size:
3001 1712 1546 273
SfcI C'TryA_G
Cuts at: 826 1045 2269 2765 3208 3396 3471 3537 3558
Size: 219 1224 496 443 188 75 66 21
Cuts at: 3558 3880 5015 5696 5887 826
Size: 322 1135 681 191 1471
Fragments arranged by size:
1471 1224 1135 681 496 443 322 219
191 188 75 66 21
SfoI GGC'GCC
Cuts at: 2230 2230
Size: 6532
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6532
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6532
SmaI CCC'GGG
Cuts at: 1872 3859 1872
Size: 1987 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4636 5537 5799 820
Size: 197 926 2693 901 262 1553
Fragments arranged by size:
2693 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4323 493
Size: 3830 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6532
SphI G_CATG'C
Cuts at: 1132 2134 3890 1132
Size: 1002 1756 3774
Fragments arranged by size:
3774 1756 1002
SspI AAT'ATT
Cuts at: 5 52 4456 5
Size: 47 4404 2081
Fragments arranged by size:
4404 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6532
StyI C'CwwG_G
Cuts at: 513 1065 2004 2083 2166 2921 3502 3971 513
Size: 552 939 79 83 755 581 469 3074
Fragments arranged by size:
3074 939 755 581 552 469 83 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2597 3248 4559 4576 4729 4914 5359
Size: 1255 1017 651 1311 17 153 185 445
Cuts at: 5359 325
Size: 1498
Fragments arranged by size:
1498 1311 1255 1017 651 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2959 3232 4778
Size: 274 80 33 51 1443 982 273 1546
Cuts at: 4778 6490 96
Size: 1712 138
Fragments arranged by size:
1712 1546 1443 982 274 273 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3305 3337 6492 6524 3305
Size: 32 3155 32 3313
Fragments arranged by size:
3313 3155 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6532
XbaI T'CTAG_A
Cuts at: 3868 3868
Size: 6532
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6532
XmaI C'CCGG_G
Cuts at: 1870 3857 1870
Size: 1987 4545
XmnI GAAnn'nnTTC
Cuts at: 2112 4661 2112
Size: 2549 3983
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2744 4340 276
Size: 53 83 186 936 1210 1596 2468
Fragments arranged by size:
2468 1596 1210 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbvCI BfrBI BplI
BsiWI BsmBI EcoICRI EcoNI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SapI SexAI SfiI
SrfI SwaI XcmI