Restriction map for
pFN21AB5988
(Circular) (Six-base) MAPSORT of: pFN21AB5988.seq Check: 2532 from: 1 to: 6379
pFN21AB5988 6379 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4189 278
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2665 2919 3559 3722 1937
Size: 728 254 640 163 4594
Fragments arranged by size:
4594 728 640 254 163
Acc65I G'GTAC_C
Cuts at: 3700 3700
Size: 6379
AclI AA'CG_TT
Cuts at: 1563 4506 4879 1563
Size: 2943 373 3063
Fragments arranged by size:
3063 2943 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2057 2141 2553 4442 5826 6094 1632
Size: 425 84 412 1889 1384 268 1917
Fragments arranged by size:
1917 1889 1384 425 412 268 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6182
AflIII A'CryG_T
Cuts at: 1203 2962 3364 5278 6255 1203
Size: 1759 402 1914 977 1327
Fragments arranged by size:
1914 1759 1327 977 402
AgeI A'CCGG_T
Cuts at: 5210 5210
Size: 6379
AhdI GACnn_n'nnGTC
Cuts at: 5108 5108
Size: 6379
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6379
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6379
AlwNI CAG_nnn'CTG
Cuts at: 3164 4122 5694 3164
Size: 958 1572 3849
Fragments arranged by size:
3849 1572 958
ApaLI G'TGCA_C
Cuts at: 4436 5592 4436
Size: 1156 5223
ApoI r'AATT_y
Cuts at: 1493 2250 2881 3083 3688 3914 4066 6175 1493
Size: 757 631 202 605 226 152 2109 1697
Fragments arranged by size:
2109 1697 757 631 605 226 202 152
AseI AT'TA_AT
Cuts at: 160 1032 3404 4933 160
Size: 872 2372 1529 1606
Fragments arranged by size:
2372 1606 1529 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
AvaI C'yCGr_G
Cuts at: 1870 1943 3704 1870
Size: 73 1761 4545
Fragments arranged by size:
4545 1761 73
BamHI G'GATC_C
Cuts at: 2829 3709 4076 2829
Size: 880 367 5132
Fragments arranged by size:
5132 880 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3700 5155 618
Size: 325 217 627 1913 1455 1842
Fragments arranged by size:
1913 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2341 2724 3698 1391
Size: 950 383 974 4072
Fragments arranged by size:
4072 974 950 383
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2181 928
Size: 971 282 5126
Fragments arranged by size:
5126 971 282
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4570 4604 1903
Size: 34 2633 34 3678
Fragments arranged by size:
3678 2633 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3610 4271 5481 3610
Size: 661 1210 4508
Fragments arranged by size:
4508 1210 661
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6379
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3641 4990 136
Size: 107 122 71 631 605 1969 1349 1525
Fragments arranged by size:
1969 1525 1349 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2737 3439 6374 2737
Size: 702 2935 2742
Fragments arranged by size:
2935 2742 702
BlpI GC'TnA_GC
Cuts at: 2379 3796 2379
Size: 1417 4962
Bme1580I G_kGCm'C
Cuts at: 1790 3010 3428 4440 5596 1790
Size: 1220 418 1012 1156 2573
Fragments arranged by size:
2573 1220 1156 1012 418
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6379
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2432 4110 5068
Size: 416 53 83 399 24 1015 1678 958
Cuts at: 5068 442
Size: 1753
Fragments arranged by size:
1753 1678 1015 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3485 1056
Size: 2429 3950
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3516 3516
Size: 6379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2883 5039 1239
Size: 1644 2156 2579
Fragments arranged by size:
2579 2156 1644
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2513 4504 5369 5667 2513
Size: 1991 865 298 3225
Fragments arranged by size:
3225 1991 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5042 882
Size: 617 3543 2219
Fragments arranged by size:
3543 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4170 493
Size: 711 2966 2702
Fragments arranged by size:
2966 2702 711
BsaBI GATnn'nnATC
Cuts at: 6373 6373
Size: 6379
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4186 4568 5203 275
Size: 53 83 186 936 2653 382 635 1451
Fragments arranged by size:
2653 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4812 5210 5484 5631 6042 4812
Size: 398 274 147 411 5149
Fragments arranged by size:
5149 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6154
Fragments arranged by size:
6154 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6379
BseYI C'CCAG_C
Cuts at: 3059 3636 5582 6078 3059
Size: 577 1946 496 3360
Fragments arranged by size:
3360 1946 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2309 3338 1163
Size: 573 573 1029 4204
Fragments arranged by size:
4204 1029 573 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3829 4590 5618 1540
Size: 275 186 1828 761 1028 2301
Fragments arranged by size:
2301 1828 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2724 3502 3541 4440 4525 5596 2724
Size: 778 39 899 85 1071 3507
Fragments arranged by size:
3507 1071 899 778 85 39
BsmI GAATG_Cn'
Cuts at: 3890 3983 3890
Size: 93 6286
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2341 2724 3010 3428 3502 3541 3698
Size: 399 551 383 286 418 74 39 157
Cuts at: 3698 4440 4525 5596 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 551 418 399 383 286
157 85 74 39
BspEI T'CCGG_A
Cuts at: 6042 6042
Size: 6379
BspHI T'CATG_A
Cuts at: 3616 4268 5998 3616
Size: 652 1730 3997
Fragments arranged by size:
3997 1730 652
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3734 844
Size: 2890 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4266 1394
Size: 429 2443 3507
Fragments arranged by size:
3507 2443 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2526 4874 5048 65
Size: 1183 1278 2348 174 1396
Fragments arranged by size:
2348 1396 1278 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5023 5210 1700
Size: 110 3213 187 2869
Fragments arranged by size:
3213 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3460 96
Size: 3364 3015
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6379
BssSI C'ACGA_G
Cuts at: 4439 5451 4439
Size: 1012 5367
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2433 2885 3005 3164 794
Size: 1639 452 120 159 4009
Fragments arranged by size:
4009 1639 452 159 120
BstBI TT'CG_AA
Cuts at: 5183 5183
Size: 6379
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6379
BstXI CCAn_nnnn'nTGG
Cuts at: 2147 2147
Size: 6379
BstYI r'GATC_y
Cuts at: 1972 2737 2829 3439 3548 3709 4076 4461 4478
Size: 765 92 610 109 161 367 385 17
Cuts at: 4478 5930 6016 6028 6374 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 765 610 385 367 346 161
109 92 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3348 3522 513
Size: 552 939 1344 174 3370
Fragments arranged by size:
3370 1344 939 552 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5640
Fragments arranged by size:
5640 683 56
BtsI GCAGTG_nn'
Cuts at: 2431 3426 3895 4689 4709 2431
Size: 995 469 794 20 4101
Fragments arranged by size:
4101 995 794 469 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6379
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6344
DraI TTT'AAA
Cuts at: 3683 3683
Size: 6379
DraIII CAC_nnn'GTG
Cuts at: 3005 3005
Size: 6379
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2185 5386 809
Size: 507 869 3201 1802
Fragments arranged by size:
3201 1802 869 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3021 3826 4715
Size: 54 1425 50 275 18 1191 805 889
Cuts at: 4715 6344 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1191 889 805 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3826 1537
Size: 275 2014 4090
Fragments arranged by size:
4090 2014 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2529 2555 4309 1353
Size: 1176 26 1754 3423
Fragments arranged by size:
3423 1754 1176 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4966 5340 5486 387
Size: 1064 138 3377 374 146 1280
Fragments arranged by size:
3377 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2722 2722
Size: 6379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2141 2553 2883 4442 5039 5826
Size: 393 425 84 412 330 1559 597 787
Cuts at: 5826 6094 1239
Size: 268 1524
Fragments arranged by size:
1559 1524 787 597 425 412 393 330
268 84
EcoRI G'AATT_C
Cuts at: 2250 3688 4066 2250
Size: 1438 378 4563
Fragments arranged by size:
4563 1438 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2339 2399 2339
Size: 60 6319
FspI TGC'GCA
Cuts at: 4885 4885
Size: 6379
HaeII r_GCGC'y
Cuts at: 1678 5526 1678
Size: 3848 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3523 3555 3584 3616 5020
Size: 32 135 32 1951 32 29 32 1404
Cuts at: 5020 5052 5094 5126 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1951 1404 135 42 32 32 32
32 32 32 29
HincII GTy'rAC
Cuts at: 669 1657 1938 2053 2178 3723 3969 669
Size: 988 281 115 125 1545 246 3079
Fragments arranged by size:
3079 1545 988 281 246 125 115
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6379
HpaI GTT'AAC
Cuts at: 3969 3969
Size: 6379
KpnI G_GTAC'C
Cuts at: 3704 3704
Size: 6379
MfeI C'AATT_G
Cuts at: 2874 3173 3978 2874
Size: 299 805 5275
Fragments arranged by size:
5275 805 299
MluI A'CGCG_T
Cuts at: 6255 6255
Size: 6379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5493 5677 1305
Size: 4188 184 2007
Fragments arranged by size:
4188 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3023 10
Size: 54 1425 343 1191 3366
Fragments arranged by size:
3366 1425 1191 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3237 4337 4696 4855 518
Size: 648 2071 1100 359 159 2042
Fragments arranged by size:
2071 2042 1100 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2048 2161 2293 2638 3059 3795 4472 5620
Size: 442 113 132 345 421 736 677 1148
Cuts at: 5620 5865 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 736 677 442 421 345 245
132 113
NcoI C'CATG_G
Cuts at: 513 1065 2004 3348 3522 513
Size: 552 939 1344 174 3370
Fragments arranged by size:
3370 1344 939 552 174
NdeI CA'TA_TG
Cuts at: 387 2351 2913 387
Size: 1964 562 3853
Fragments arranged by size:
3853 1964 562
NheI G'CTAG_C
Cuts at: 1052 3481 1052
Size: 2429 3950
NotI GC'GGCC_GC
Cuts at: 3826 3826
Size: 6379
NspI r_CATG'y
Cuts at: 1132 1229 2966 2996 3368 3737 5282 1132
Size: 97 1737 30 372 369 1545 2229
Fragments arranged by size:
2229 1737 1545 372 369 97 30
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6379
PciI A'CATG_T
Cuts at: 2962 3364 5278 2962
Size: 402 1914 4063
Fragments arranged by size:
4063 1914 402
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6379
PmeI GTTT'AAAC
Cuts at: 3683 3683
Size: 6379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4448 4480 5961 5993 4448
Size: 32 1481 32 4834
Fragments arranged by size:
4834 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3265 1613
Size: 1652 4727
PsiI TTA'TAA
Cuts at: 3035 3949 3035
Size: 914 5465
PstI C_TGCA'G
Cuts at: 830 3731 830
Size: 2901 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6379
PvuII CAG'CTG
Cuts at: 2048 2161 2293 2638 3059 2048
Size: 113 132 345 421 5368
Fragments arranged by size:
5368 421 345 132 113
SacI G_AGCT'C
Cuts at: 2724 2724
Size: 6379
SalI G'TCGA_C
Cuts at: 1936 3721 1936
Size: 1785 4594
SapI GCTCTTCn'nnn_
Cuts at: 2529 2529
Size: 6379
SbfI CC_TGCA'GG
Cuts at: 3731 3731
Size: 6379
ScaI AGT'ACT
Cuts at: 4627 6339 4627
Size: 1712 4667
SfcI C'TryA_G
Cuts at: 826 1045 2633 2852 3727 4862 5543 5734 826
Size: 219 1588 219 875 1135 681 191 1471
Fragments arranged by size:
1588 1471 1135 875 681 219 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6379
SmaI CCC'GGG
Cuts at: 1872 3706 1872
Size: 1834 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2492 4483 5384 5646 820
Size: 197 926 549 1991 901 262 1553
Fragments arranged by size:
1991 1553 926 901 549 262 197
SnaBI TAC'GTA
Cuts at: 493 4170 493
Size: 3677 2702
SpeI A'CTAG_T
Cuts at: 152 3356 152
Size: 3204 3175
SphI G_CATG'C
Cuts at: 1132 2996 3737 1132
Size: 1864 741 3774
Fragments arranged by size:
3774 1864 741
SspI AAT'ATT
Cuts at: 5 52 4303 5
Size: 47 4251 2081
Fragments arranged by size:
4251 2081 47
StuI AGG'CCT
Cuts at: 1517 2652 3644 1517
Size: 1135 992 4252
Fragments arranged by size:
4252 1135 992
StyI C'CwwG_G
Cuts at: 513 1065 2004 3348 3522 3818 513
Size: 552 939 1344 174 296 3074
Fragments arranged by size:
3074 1344 939 552 296 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2384 4406 4423 4576 4761 5206 325
Size: 1255 804 2022 17 153 185 445 1498
Fragments arranged by size:
2022 1498 1255 804 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2519 2986 3460
Size: 274 80 33 51 1443 542 467 474
Cuts at: 3460 3571 4625 6337 96
Size: 111 1054 1712 138
Fragments arranged by size:
1712 1443 1054 542 474 467 274 138
111 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6339 6371 6339
Size: 32 6347
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6379
XbaI T'CTAG_A
Cuts at: 3442 3589 3715 3442
Size: 147 126 6106
Fragments arranged by size:
6106 147 126
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6379
XmaI C'CCGG_G
Cuts at: 1870 3704 1870
Size: 1834 4545
XmnI GAAnn'nnTTC
Cuts at: 4508 4508
Size: 6379
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4187 276
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI