Restriction map for
pFN21AB5987
(Circular) (Six-base) MAPSORT of: pFN21AB5987.seq Check: 2164 from: 1 to: 6415
pFN21AB5987 6415 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3622 3622
Size: 6415
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4225 278
Size: 53 83 186 936 2689 2468
Fragments arranged by size:
2689 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3758 1937
Size: 1821 4594
Acc65I G'GTAC_C
Cuts at: 3736 3736
Size: 6415
AclI AA'CG_TT
Cuts at: 1563 4542 4915 1563
Size: 2979 373 3063
Fragments arranged by size:
3063 2979 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3566 4478 5862 6130 1632
Size: 1934 912 1384 268 1917
Fragments arranged by size:
1934 1917 1384 912 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6218
AflIII A'CryG_T
Cuts at: 1203 2636 5314 6291 1203
Size: 1433 2678 977 1327
Fragments arranged by size:
2678 1433 1327 977
AgeI A'CCGG_T
Cuts at: 5246 5246
Size: 6415
AhdI GACnn_n'nnGTC
Cuts at: 5144 5144
Size: 6415
AleI CACnn'nnGTG
Cuts at: 1166 2014 1166
Size: 848 5567
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2076 1184
Size: 892 5523
AlwNI CAG_nnn'CTG
Cuts at: 3618 4158 5730 3618
Size: 540 1572 4303
Fragments arranged by size:
4303 1572 540
ApaLI G'TGCA_C
Cuts at: 4472 5628 4472
Size: 1156 5259
ApoI r'AATT_y
Cuts at: 1493 2168 2560 3260 3299 3724 3950 4102 6211
Size: 675 392 700 39 425 226 152 2109
Cuts at: 6211 1493
Size: 1697
Fragments arranged by size:
2109 1697 700 675 425 392 226 152
39
AseI AT'TA_AT
Cuts at: 160 1032 4969 160
Size: 872 3937 1606
Fragments arranged by size:
3937 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6415
AvaI C'yCGr_G
Cuts at: 1870 1943 2267 3480 3740 1870
Size: 73 324 1213 260 4545
Fragments arranged by size:
4545 1213 324 260 73
BamHI G'GATC_C
Cuts at: 3745 4112 3745
Size: 367 6048
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3736 5191 618
Size: 325 217 627 1949 1455 1842
Fragments arranged by size:
1949 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3178 3453 3734 1391
Size: 1787 275 281 4072
Fragments arranged by size:
4072 1787 281 275
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5444
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4606 4640 1903
Size: 34 2669 34 3678
Fragments arranged by size:
3678 2669 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3241 3299 3612 4307 5517 3241
Size: 58 313 695 1210 4139
Fragments arranged by size:
4139 1210 695 313 58
BclI T'GATC_A
Cuts at: 1548 2752 1548
Size: 1204 5211
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5026 136
Size: 107 122 71 631 605 3354 1525
Fragments arranged by size:
3354 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6410 6410
Size: 6415
BlpI GC'TnA_GC
Cuts at: 3832 3832
Size: 6415
Bme1580I G_kGCm'C
Cuts at: 1790 2147 2730 4476 5632 1790
Size: 357 583 1746 1156 2573
Fragments arranged by size:
2573 1746 1156 583 357
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6415
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2092 2432 4146
Size: 416 53 83 399 24 675 340 1714
Cuts at: 4146 5104 442
Size: 958 1753
Fragments arranged by size:
1753 1714 958 675 416 399 340 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3410 3467 5075 1239
Size: 2171 57 1608 2579
Fragments arranged by size:
2579 2171 1608 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2261 2614 2966 3437 4540 5405 5703 2261
Size: 353 352 471 1103 865 298 2973
Fragments arranged by size:
2973 1103 865 471 353 352 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5078 882
Size: 617 3579 2219
Fragments arranged by size:
3579 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3421 4206 493
Size: 711 2217 785 2702
Fragments arranged by size:
2702 2217 785 711
BsaBI GATnn'nnATC
Cuts at: 6409 6409
Size: 6415
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3391 4222 4604 5239
Size: 53 83 186 936 1858 831 382 635
Cuts at: 5239 275
Size: 1451
Fragments arranged by size:
1858 1451 936 831 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4848 5246 5520 5667 6078 4848
Size: 398 274 147 411 5185
Fragments arranged by size:
5185 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3090 3120 3261 3291 1178
Size: 30 165 30 1687 30 141 30 4302
Fragments arranged by size:
4302 1687 165 141 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2258 2309 2798 1186
Size: 1072 51 489 4803
Fragments arranged by size:
4803 1072 489 51
BseYI C'CCAG_C
Cuts at: 5618 6114 5618
Size: 496 5919
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5842
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3865 4626 5654 1540
Size: 275 186 1864 761 1028 2301
Fragments arranged by size:
2301 1864 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3426 3453 3567 4476 4561 5632 3426
Size: 27 114 909 85 1071 4209
Fragments arranged by size:
4209 1071 909 114 85 27
BsiWI C'GTAC_G
Cuts at: 2666 2666
Size: 6415
BsmI GAATG_Cn'
Cuts at: 2466 3250 3926 4019 2466
Size: 784 676 93 4862
Fragments arranged by size:
4862 784 676 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2147 2730 3178 3426 3453 3567 3734
Size: 399 357 583 448 248 27 114 167
Cuts at: 3734 4476 4561 5632 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 583 448 399 357 248
167 114 85 27
BspEI T'CCGG_A
Cuts at: 6078 6078
Size: 6415
BspHI T'CATG_A
Cuts at: 4304 6034 4304
Size: 1730 4685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2351 3603 3622 3770 844
Size: 1507 1252 19 148 3489
Fragments arranged by size:
3489 1507 1252 148 19
BsrBI CCG'CTC
Cuts at: 1394 1823 4302 1394
Size: 429 2479 3507
Fragments arranged by size:
3507 2479 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2604 2842 4910 5084 65
Size: 1183 1356 238 2068 174 1396
Fragments arranged by size:
2068 1396 1356 1183 238 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5059 5246 1700
Size: 110 3249 187 2869
Fragments arranged by size:
3249 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6415
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6415
BssSI C'ACGA_G
Cuts at: 4475 5487 4475
Size: 1012 5403
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2142 3055 3245 794
Size: 1348 913 190 3964
Fragments arranged by size:
3964 1348 913 190
BstBI TT'CG_AA
Cuts at: 5219 5219
Size: 6415
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6415
BstXI CCAn_nnnn'nTGG
Cuts at: 2195 2195
Size: 6415
BstYI r'GATC_y
Cuts at: 1972 2977 3745 4112 4497 4514 5966 6052 6064
Size: 1005 768 367 385 17 1452 86 12
Cuts at: 6064 6410 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1005 768 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3280 513
Size: 552 2215 3648
Fragments arranged by size:
3648 2215 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2091 487
Size: 683 56 865 4811
Fragments arranged by size:
4811 865 683 56
BtsI GCAGTG_nn'
Cuts at: 2730 3679 3931 4725 4745 2730
Size: 949 252 794 20 4400
Fragments arranged by size:
4400 949 794 252 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6415
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6380
DraI TTT'AAA
Cuts at: 3719 3719
Size: 6415
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5422 809
Size: 507 4106 1802
Fragments arranged by size:
4106 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3636 3862 4751
Size: 54 1425 50 275 18 1806 226 889
Cuts at: 4751 6380 8
Size: 1629 43
Fragments arranged by size:
1806 1629 1425 889 275 226 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3862 1537
Size: 275 2050 4090
Fragments arranged by size:
4090 2050 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3542 4345 1353
Size: 2189 803 3423
Fragments arranged by size:
3423 2189 803
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2249 5002 5376 5522 387
Size: 1064 138 660 2753 374 146 1280
Fragments arranged by size:
2753 1280 1064 660 374 146 138
EcoICRI GAG'CTC
Cuts at: 3451 3451
Size: 6415
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3410 3467 3566 4478 5075 5862 6130
Size: 393 1778 57 99 912 597 787 268
Cuts at: 6130 1239
Size: 1524
Fragments arranged by size:
1778 1524 912 787 597 393 268 99
57
EcoNI CCTnn'n_nnAGG
Cuts at: 3238 3238
Size: 6415
EcoO109I rG'GnC_Cy
Cuts at: 2236 3685 2236
Size: 1449 4966
EcoRI G'AATT_C
Cuts at: 3724 4102 3724
Size: 378 6037
FspI TGC'GCA
Cuts at: 4921 4921
Size: 6415
HaeII r_GCGC'y
Cuts at: 1678 5562 1678
Size: 3884 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2467 2499 2815 2847 3090
Size: 32 135 32 895 32 316 32 243
Cuts at: 3090 3122 5056 5088 5130 5162 1373
Size: 32 1934 32 42 32 2626
Fragments arranged by size:
2626 1934 895 316 243 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3759 4005 669
Size: 988 281 1821 246 3079
Fragments arranged by size:
3079 1821 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6415
HpaI GTT'AAC
Cuts at: 4005 4005
Size: 6415
KpnI G_GTAC'C
Cuts at: 3740 3740
Size: 6415
MfeI C'AATT_G
Cuts at: 4014 4014
Size: 6415
MluI A'CGCG_T
Cuts at: 6291 6291
Size: 6415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5529 5713 1305
Size: 4224 184 2007
Fragments arranged by size:
4224 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3638 10
Size: 54 1425 343 1806 2787
Fragments arranged by size:
2787 1806 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2014 3644 4373 4732 4891 518
Size: 648 848 1630 729 359 159 2042
Fragments arranged by size:
2042 1630 848 729 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2195 2412 3628 3831 4508 5656 5901 1606
Size: 589 217 1216 203 677 1148 245 2120
Fragments arranged by size:
2120 1216 1148 677 589 245 217 203
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5863
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6415
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6415
NotI GC'GGCC_GC
Cuts at: 3862 3862
Size: 6415
NspI r_CATG'y
Cuts at: 1132 1229 2350 2640 3773 5318 1132
Size: 97 1121 290 1133 1545 2229
Fragments arranged by size:
2229 1545 1133 1121 290 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6415
PciI A'CATG_T
Cuts at: 2636 5314 2636
Size: 2678 3737
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2660 1108
Size: 1552 4863
PfoI T'CCnGG_A
Cuts at: 3444 3444
Size: 6415
PmeI GTTT'AAAC
Cuts at: 3719 3719
Size: 6415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4484 4516 5997 6029 4484
Size: 32 1481 32 4870
Fragments arranged by size:
4870 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2236 2236
Size: 6415
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6415
PsiI TTA'TAA
Cuts at: 2550 3985 2550
Size: 1435 4980
PstI C_TGCA'G
Cuts at: 830 3633 3767 830
Size: 2803 134 3478
Fragments arranged by size:
3478 2803 134
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6415
PvuII CAG'CTG
Cuts at: 2195 2412 3628 2195
Size: 217 1216 4982
Fragments arranged by size:
4982 1216 217
SacI G_AGCT'C
Cuts at: 3453 3453
Size: 6415
SalI G'TCGA_C
Cuts at: 1936 3757 1936
Size: 1821 4594
SbfI CC_TGCA'GG
Cuts at: 3767 3767
Size: 6415
ScaI AGT'ACT
Cuts at: 4663 6375 4663
Size: 1712 4703
SfcI C'TryA_G
Cuts at: 826 1045 2176 3629 3763 4898 5579 5770 826
Size: 219 1131 1453 134 1135 681 191 1471
Fragments arranged by size:
1471 1453 1135 1131 681 219 191 134
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6415
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6415
SmaI CCC'GGG
Cuts at: 1872 3742 1872
Size: 1870 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2240 2593 2981 3452 4519 5420
Size: 197 926 297 353 388 471 1067 901
Cuts at: 5420 5682 820
Size: 262 1553
Fragments arranged by size:
1553 1067 926 901 471 388 353 297
262 197
SnaBI TAC'GTA
Cuts at: 493 4206 493
Size: 3713 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6415
SphI G_CATG'C
Cuts at: 1132 3773 1132
Size: 2641 3774
SspI AAT'ATT
Cuts at: 5 52 4339 5
Size: 47 4287 2081
Fragments arranged by size:
4287 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6415
StyI C'CwwG_G
Cuts at: 513 1065 2388 3039 3854 513
Size: 552 1323 651 815 3074
Fragments arranged by size:
3074 1323 815 651 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3342 4442 4459 4612 4797 5242 325
Size: 1255 1762 1100 17 153 185 445 1498
Fragments arranged by size:
1762 1498 1255 1100 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3505 3510 4661
Size: 274 80 33 51 1443 1528 5 1151
Cuts at: 4661 6373 96
Size: 1712 138
Fragments arranged by size:
1712 1528 1443 1151 274 138 80 51
33 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6375 6407 6375
Size: 32 6383
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6415
XbaI T'CTAG_A
Cuts at: 3751 3751
Size: 6415
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6415
XmaI C'CCGG_G
Cuts at: 1870 3740 1870
Size: 1870 4545
XmnI GAAnn'nnTTC
Cuts at: 2397 4544 2397
Size: 2147 4268
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4223 276
Size: 53 83 186 936 2689 2468
Fragments arranged by size:
2689 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI