Restriction map for
pFN21AB5981
(Circular) (Six-base) MAPSORT of: pFN21AB5981.seq Check: 1435 from: 1 to: 6088
pFN21AB5981 6088 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3898 278
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2638 3431 1937
Size: 701 793 4594
Fragments arranged by size:
4594 793 701
Acc65I G'GTAC_C
Cuts at: 3409 3409
Size: 6088
AclI AA'CG_TT
Cuts at: 1563 4215 4588 1563
Size: 2652 373 3063
Fragments arranged by size:
3063 2652 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2098 2236 2683 3103 3187 3271 3351 4151
Size: 466 138 447 420 84 84 80 800
Cuts at: 4151 5535 5803 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 800 466 447 420 268 138
84 84 80
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5891
AflIII A'CryG_T
Cuts at: 1203 2423 2559 4987 5964 1203
Size: 1220 136 2428 977 1327
Fragments arranged by size:
2428 1327 1220 977 136
AgeI A'CCGG_T
Cuts at: 4919 4919
Size: 6088
AhdI GACnn_n'nnGTC
Cuts at: 4817 4817
Size: 6088
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6088
AlwNI CAG_nnn'CTG
Cuts at: 3831 5403 3831
Size: 1572 4516
ApaLI G'TGCA_C
Cuts at: 4145 5301 4145
Size: 1156 4932
ApoI r'AATT_y
Cuts at: 1493 2175 3153 3321 3397 3623 3775 5884 1493
Size: 682 978 168 76 226 152 2109 1697
Fragments arranged by size:
2109 1697 978 682 226 168 152 76
AseI AT'TA_AT
Cuts at: 160 1032 4642 160
Size: 872 3610 1606
Fragments arranged by size:
3610 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
AvaI C'yCGr_G
Cuts at: 1870 1943 3413 1870
Size: 73 1470 4545
Fragments arranged by size:
4545 1470 73
BamHI G'GATC_C
Cuts at: 2068 3418 3785 2068
Size: 1350 367 4371
Fragments arranged by size:
4371 1350 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3409 4864 618
Size: 325 217 627 1622 1455 1842
Fragments arranged by size:
1842 1622 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3407 1391
Size: 2016 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2154 2169 2560 2980 928
Size: 971 255 15 391 420 4036
Fragments arranged by size:
4036 971 420 391 255 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4279 4313 1903
Size: 34 2342 34 3678
Fragments arranged by size:
3678 2342 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3980 5190 3980
Size: 1210 4878
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6088
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4699 136
Size: 107 122 71 631 605 3027 1525
Fragments arranged by size:
3027 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6083 6083
Size: 6088
BlpI GC'TnA_GC
Cuts at: 3505 3505
Size: 6088
Bme1580I G_kGCm'C
Cuts at: 1790 4149 5305 1790
Size: 2359 1156 2573
Fragments arranged by size:
2573 2359 1156
BmgBI CAC'GTC
Cuts at: 1318 2381 1318
Size: 1063 5025
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2619 3039 3371
Size: 416 53 83 399 24 1202 420 332
Cuts at: 3371 3819 4777 442
Size: 448 958 1753
Fragments arranged by size:
1753 1202 958 448 420 416 399 332
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2313 2511 2679 2763 3099 3183 3267
Size: 830 244 198 168 84 336 84 84
Cuts at: 3267 3351 4748 1239
Size: 84 1397 2579
Fragments arranged by size:
2579 1397 830 336 244 198 168 84
84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2467 4213 5078 5376 2467
Size: 1746 865 298 3179
Fragments arranged by size:
3179 1746 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4751 882
Size: 617 3252 2219
Fragments arranged by size:
3252 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2426 2474 3879 493
Size: 711 1222 48 1405 2702
Fragments arranged by size:
2702 1405 1222 711 48
BsaBI GATnn'nnATC
Cuts at: 6082 6082
Size: 6088
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3895 4277 4912 275
Size: 53 83 186 936 2362 382 635 1451
Fragments arranged by size:
2362 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2909 4521 4919 5193 5340 5751 2909
Size: 1612 398 274 147 411 3246
Fragments arranged by size:
3246 1612 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5863
Fragments arranged by size:
5863 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 1186
Size: 880 5208
BseYI C'CCAG_C
Cuts at: 5291 5787 5291
Size: 496 5592
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5515
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3538 4299 5327 1540
Size: 275 186 1537 761 1028 2301
Fragments arranged by size:
2301 1537 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4149 4234 5305 4149
Size: 85 1071 4932
Fragments arranged by size:
4932 1071 85
BsmI GAATG_Cn'
Cuts at: 2547 2681 2765 2942 3599 3692 2547
Size: 134 84 177 657 93 4943
Fragments arranged by size:
4943 657 177 134 93 84
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3407 4149 4234 5305 1391
Size: 399 1617 742 85 1071 2174
Fragments arranged by size:
2174 1617 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5751 5751
Size: 6088
BspHI T'CATG_A
Cuts at: 3977 5707 3977
Size: 1730 4358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3443 844
Size: 2599 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3975 1394
Size: 429 2152 3507
Fragments arranged by size:
3507 2152 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2690 3004 3110 3278 4583 4757 65
Size: 1183 1442 314 106 168 1305 174 1396
Fragments arranged by size:
1442 1396 1305 1183 314 174 168 106
BsrFI r'CCGG_y
Cuts at: 1700 1810 4732 4919 1700
Size: 110 2922 187 2869
Fragments arranged by size:
2922 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3182 96
Size: 3086 3002
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6088
BssSI C'ACGA_G
Cuts at: 2894 4148 5160 2894
Size: 1254 1012 3822
Fragments arranged by size:
3822 1254 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6088
BstBI TT'CG_AA
Cuts at: 2551 2803 2887 3055 3139 4892 2551
Size: 252 84 168 84 1753 3747
Fragments arranged by size:
3747 1753 252 168 84 84
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6088
BstYI r'GATC_y
Cuts at: 1972 2068 3418 3785 4170 4187 5639 5725 5737
Size: 96 1350 367 385 17 1452 86 12
Cuts at: 5737 6083 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1350 385 367 346 96 86
17 12
BstZ17I GTA'TAC
Cuts at: 2639 2639
Size: 6088
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4597
Fragments arranged by size:
4597 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2441 487
Size: 683 56 1215 4134
Fragments arranged by size:
4134 1215 683 56
BtsI GCAGTG_nn'
Cuts at: 2320 2774 3604 4398 4418 2320
Size: 454 830 794 20 3990
Fragments arranged by size:
3990 830 794 454 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6088
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2668 2703 2752 2787 529
Size: 35 2104 35 49 35 3830
Fragments arranged by size:
3830 2104 49 35 35 35
DraI TTT'AAA
Cuts at: 3392 3392
Size: 6088
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5095 809
Size: 507 3779 1802
Fragments arranged by size:
3779 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3535 4424 6053
Size: 54 1425 50 275 18 1705 889 1629
Cuts at: 6053 8
Size: 43
Fragments arranged by size:
1705 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3535 1537
Size: 275 1723 4090
Fragments arranged by size:
4090 1723 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4018 1353
Size: 2665 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4675 5049 5195 387
Size: 1064 138 3086 374 146 1280
Fragments arranged by size:
3086 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2098 2236 2313 2511 2679 2683
Size: 393 437 29 138 77 198 168 4
Cuts at: 2683 2763 3099 3103 3183 3187 3267 3271 3351
Size: 80 336 4 80 4 80 4 80
Cuts at: 3351 4151 4748 5535 5803 1239
Size: 800 597 787 268 1524
Fragments arranged by size:
1524 800 787 597 437 393 336 268
198 168 138 80 80 80 80 77
29 4 4 4 4
EcoO109I rG'GnC_Cy
Cuts at: 2339 3069 2339
Size: 730 5358
EcoRI G'AATT_C
Cuts at: 3153 3397 3775 3153
Size: 244 378 5466
Fragments arranged by size:
5466 378 244
FspI TGC'GCA
Cuts at: 4594 4594
Size: 6088
HaeII r_GCGC'y
Cuts at: 1678 5235 1678
Size: 3557 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2440 2472 4729 4761 4803
Size: 32 135 32 868 32 2257 32 42
Cuts at: 4803 4835 1373
Size: 32 2626
Fragments arranged by size:
2626 2257 868 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2565 3432 3678 669
Size: 988 281 627 867 246 3079
Fragments arranged by size:
3079 988 867 627 281 246
HindIII A'AGCT_T
Cuts at: 748 2778 748
Size: 2030 4058
HpaI GTT'AAC
Cuts at: 2565 3678 2565
Size: 1113 4975
KpnI G_GTAC'C
Cuts at: 3413 3413
Size: 6088
MfeI C'AATT_G
Cuts at: 3687 3687
Size: 6088
MluI A'CGCG_T
Cuts at: 5964 5964
Size: 6088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5202 5386 1305
Size: 3897 184 2007
Fragments arranged by size:
3897 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4266
Fragments arranged by size:
4266 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4046 4405 4564 518
Size: 648 2880 359 159 2042
Fragments arranged by size:
2880 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3504 4181 5329 5574 1606
Size: 1898 677 1148 245 2120
Fragments arranged by size:
2120 1898 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4597
Fragments arranged by size:
4597 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6088
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6088
NotI GC'GGCC_GC
Cuts at: 3535 3535
Size: 6088
NspI r_CATG'y
Cuts at: 1132 1229 2563 3446 4991 1132
Size: 97 1334 883 1545 2229
Fragments arranged by size:
2229 1545 1334 883 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6088
PciI A'CATG_T
Cuts at: 2559 4987 2559
Size: 2428 3660
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6088
PfoI T'CCnGG_A
Cuts at: 2290 2488 2290
Size: 198 5890
PmeI GTTT'AAAC
Cuts at: 3392 3392
Size: 6088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4157 4189 5670 5702 4157
Size: 32 1481 32 4543
Fragments arranged by size:
4543 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2339 3069 2339
Size: 730 5358
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6088
PsiI TTA'TAA
Cuts at: 3009 3658 3009
Size: 649 5439
PstI C_TGCA'G
Cuts at: 830 3440 830
Size: 2610 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6088
SalI G'TCGA_C
Cuts at: 1936 3430 1936
Size: 1494 4594
SbfI CC_TGCA'GG
Cuts at: 3440 3440
Size: 6088
ScaI AGT'ACT
Cuts at: 4336 6048 4336
Size: 1712 4376
SfcI C'TryA_G
Cuts at: 826 1045 2089 2130 3436 4571 5252 5443 826
Size: 219 1044 41 1306 1135 681 191 1471
Fragments arranged by size:
1471 1306 1135 1044 681 219 191 41
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6088
SmaI CCC'GGG
Cuts at: 1872 3415 1872
Size: 1543 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2446 4192 5093 5355 820
Size: 197 926 503 1746 901 262 1553
Fragments arranged by size:
1746 1553 926 901 503 262 197
SnaBI TAC'GTA
Cuts at: 493 3879 493
Size: 3386 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6088
SphI G_CATG'C
Cuts at: 1132 3446 1132
Size: 2314 3774
SspI AAT'ATT
Cuts at: 5 52 4012 5
Size: 47 3960 2081
Fragments arranged by size:
3960 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6088
StyI C'CwwG_G
Cuts at: 513 1065 2004 3527 513
Size: 552 939 1523 3074
Fragments arranged by size:
3074 1523 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4115 4132 4285 4470 4915 325
Size: 1255 2535 17 153 185 445 1498
Fragments arranged by size:
2535 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2796 3182 3310
Size: 274 80 33 51 1443 819 386 128
Cuts at: 3310 4334 6046 96
Size: 1024 1712 138
Fragments arranged by size:
1712 1443 1024 819 386 274 138 128
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6048 6080 6048
Size: 32 6056
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6088
XbaI T'CTAG_A
Cuts at: 3424 3424
Size: 6088
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2264 2011
Size: 253 5835
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6088
XmaI C'CCGG_G
Cuts at: 1870 3413 1870
Size: 1543 4545
XmnI GAAnn'nnTTC
Cuts at: 2696 2780 3116 3153 3200 3284 3321 4217 2696
Size: 84 336 37 47 84 37 896 4567
Fragments arranged by size:
4567 896 336 84 84 47 37 37
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3896 276
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI