Restriction map for
pFN21AB5980
(Circular) (Six-base) MAPSORT of: pFN21AB5980.seq Check: 3043 from: 1 to: 6406
pFN21AB5980 6406 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2575 2575
Size: 6406
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4216 278
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3749 1937
Size: 1812 4594
Acc65I G'GTAC_C
Cuts at: 2238 3727 2238
Size: 1489 4917
AclI AA'CG_TT
Cuts at: 1563 4533 4906 1563
Size: 2970 373 3063
Fragments arranged by size:
3063 2970 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2437 2940 4469 5853 6121 1632
Size: 805 503 1529 1384 268 1917
Fragments arranged by size:
1917 1529 1384 805 503 268
AfeI AGC'GCT
Cuts at: 2312 2312
Size: 6406
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6209
AflIII A'CryG_T
Cuts at: 1203 2223 5305 6282 1203
Size: 1020 3082 977 1327
Fragments arranged by size:
3082 1327 1020 977
AgeI A'CCGG_T
Cuts at: 2119 2783 5237 2119
Size: 664 2454 3288
Fragments arranged by size:
3288 2454 664
AhdI GACnn_n'nnGTC
Cuts at: 5135 5135
Size: 6406
AleI CACnn'nnGTG
Cuts at: 1166 2120 1166
Size: 954 5452
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6406
AlwNI CAG_nnn'CTG
Cuts at: 2134 2551 2590 2878 4149 5721 2134
Size: 417 39 288 1271 1572 2819
Fragments arranged by size:
2819 1572 1271 417 288 39
ApaI G_GGCC'C
Cuts at: 2085 2085
Size: 6406
ApaLI G'TGCA_C
Cuts at: 3251 4463 5619 3251
Size: 1212 1156 4038
Fragments arranged by size:
4038 1212 1156
ApoI r'AATT_y
Cuts at: 1493 3715 3941 4093 6202 1493
Size: 2222 226 152 2109 1697
Fragments arranged by size:
2222 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4960 160
Size: 872 3928 1606
Fragments arranged by size:
3928 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
AvaI C'yCGr_G
Cuts at: 1870 1943 2084 2092 2648 2969 3016 3501 3731
Size: 73 141 8 556 321 47 485 230
Cuts at: 3731 1870
Size: 4545
Fragments arranged by size:
4545 556 485 321 230 141 73 47
8
BamHI G'GATC_C
Cuts at: 3736 4103 3736
Size: 367 6039
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 2238 2688 2728 2975
Size: 325 217 627 335 116 450 40 247
Cuts at: 2975 3161 3314 3727 5182 618
Size: 186 153 413 1455 1842
Fragments arranged by size:
1842 1455 627 450 413 335 325 247
217 186 153 116 40
BanII G_rGCy'C
Cuts at: 1391 2085 2099 2153 3047 3195 3725 1391
Size: 694 14 54 894 148 530 4072
Fragments arranged by size:
4072 894 694 530 148 54 14
BbeI G_GCGC'C
Cuts at: 2692 2732 2979 3318 2692
Size: 40 247 339 5780
Fragments arranged by size:
5780 339 247 40
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2178 3276 3417 928
Size: 971 279 1098 141 3917
Fragments arranged by size:
3917 1098 971 279 141
BbvCI CC'TCA_GC
Cuts at: 2907 3035 2907
Size: 128 6278
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3139 3173 3346 3380 4597 4631 1903
Size: 34 1202 34 173 34 1217 34 3678
Fragments arranged by size:
3678 1217 1202 173 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4298 5508 4298
Size: 1210 5196
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6406
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2148 2891 2903
Size: 107 122 71 631 605 476 743 12
Cuts at: 2903 3359 5017 136
Size: 456 1658 1525
Fragments arranged by size:
1658 1525 743 631 605 476 456 122
107 71 12
BglII A'GATC_T
Cuts at: 6401 6401
Size: 6406
BlpI GC'TnA_GC
Cuts at: 3823 3823
Size: 6406
Bme1580I G_kGCm'C
Cuts at: 1790 2085 2562 3255 4467 5623 1790
Size: 295 477 693 1212 1156 2573
Fragments arranged by size:
2573 1212 1156 693 477 295
BmgBI CAC'GTC
Cuts at: 1318 2224 1318
Size: 906 5500
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2267 2403 3565
Size: 416 53 83 399 24 850 136 1162
Cuts at: 3565 4137 5095 442
Size: 572 958 1753
Fragments arranged by size:
1753 1162 958 850 572 416 399 136
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2516 2870 2900 3209 3364 3426 3668 5066
Size: 1277 354 30 309 155 62 242 1398
Cuts at: 5066 1239
Size: 2579
Fragments arranged by size:
2579 1398 1277 354 309 242 155 62
30
Bpu10I CC'TnA_GC
Cuts at: 2907 3035 2907
Size: 128 6278
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2941 3533 4531 5396 5694 2941
Size: 592 998 865 298 3653
Fragments arranged by size:
3653 998 865 592 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2420 3119 5069 882
Size: 617 921 699 1950 2219
Fragments arranged by size:
2219 1950 921 699 617
BsaAI yAC'GTr
Cuts at: 493 1204 2245 4197 493
Size: 711 1041 1952 2702
Fragments arranged by size:
2702 1952 1041 711
BsaBI GATnn'nnATC
Cuts at: 3215 3221 6400 3215
Size: 6 3179 3221
Fragments arranged by size:
3221 3179 6
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2689 2729 2976 3315
Size: 53 83 186 936 1156 40 247 339
Cuts at: 3315 4213 4595 5230 275
Size: 898 382 635 1451
Fragments arranged by size:
1451 1156 936 898 635 382 339 247
186 83 53 40
BsaWI w'CCGG_w
Cuts at: 2119 2783 3516 4839 5237 5511 5658 6069 2119
Size: 664 733 1323 398 274 147 411 2456
Fragments arranged by size:
2456 1323 733 664 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2466 2496 1178
Size: 30 165 30 1063 30 5088
Fragments arranged by size:
5088 1063 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2507 2867 3537 1186
Size: 1321 360 670 4055
Fragments arranged by size:
4055 1321 670 360
BseYI C'CCAG_C
Cuts at: 2262 2292 2503 2995 3246 3472 5609 6105 2262
Size: 30 211 492 251 226 2137 496 2563
Fragments arranged by size:
2563 2137 496 492 251 226 211 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2576 1163
Size: 573 840 4993
Fragments arranged by size:
4993 840 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3028 3856 4617 5645 1540
Size: 275 186 1027 828 761 1028 2301
Fragments arranged by size:
2301 1028 1027 828 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3255 3350 4467 4552 5623 3255
Size: 95 1117 85 1071 4038
Fragments arranged by size:
4038 1117 1071 95 85
BsmI GAATG_Cn'
Cuts at: 3400 3917 4010 3400
Size: 517 93 5796
Fragments arranged by size:
5796 517 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2099 2153 2562 3047 3195 3255
Size: 399 295 14 54 409 485 148 60
Cuts at: 3255 3350 3725 4467 4552 5623 1391
Size: 95 375 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 485 409 399 375 295
148 95 85 60 54 14
BspEI T'CCGG_A
Cuts at: 6069 6069
Size: 6406
BspHI T'CATG_A
Cuts at: 4295 6025 4295
Size: 1730 4676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2575 2877 2975 3761 844
Size: 1731 302 98 786 3489
Fragments arranged by size:
3489 1731 786 302 98
BsrBI CCG'CTC
Cuts at: 1394 1823 2745 2857 4293 1394
Size: 429 922 112 1436 3507
Fragments arranged by size:
3507 1436 922 429 112
BsrDI GCAATG_nn'
Cuts at: 65 1248 4901 5075 65
Size: 1183 3653 174 1396
Fragments arranged by size:
3653 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2119 2783 5050 5237 1700
Size: 110 309 664 2267 187 2869
Fragments arranged by size:
2869 2267 664 309 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6406
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6406
BssSI C'ACGA_G
Cuts at: 4466 5478 4466
Size: 1012 5394
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2590 3243 3354 794
Size: 1796 653 111 3846
Fragments arranged by size:
3846 1796 653 111
BstBI TT'CG_AA
Cuts at: 5210 5210
Size: 6406
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6406
BstYI r'GATC_y
Cuts at: 1972 2457 3736 4103 4488 4505 5957 6043 6055
Size: 485 1279 367 385 17 1452 86 12
Cuts at: 6055 6401 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1279 485 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3055 3055
Size: 6406
BtgI C'CryG_G
Cuts at: 513 1065 2706 2886 513
Size: 552 1641 180 4033
Fragments arranged by size:
4033 1641 552 180
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3421 487
Size: 683 56 2195 3472
Fragments arranged by size:
3472 2195 683 56
BtsI GCAGTG_nn'
Cuts at: 2757 3922 4716 4736 2757
Size: 1165 794 20 4427
Fragments arranged by size:
4427 1165 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6406
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6371
DraI TTT'AAA
Cuts at: 3710 3710
Size: 6406
DraIII CAC_nnn'GTG
Cuts at: 2209 2248 2619 2712 3448 2209
Size: 39 371 93 736 5167
Fragments arranged by size:
5167 736 371 93 39
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5413 809
Size: 507 4097 1802
Fragments arranged by size:
4097 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2112 3025 3372
Size: 54 1425 50 275 18 282 913 347
Cuts at: 3372 3853 4742 6371 8
Size: 481 889 1629 43
Fragments arranged by size:
1629 1425 913 889 481 347 282 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3025 3853 1537
Size: 275 1213 828 4090
Fragments arranged by size:
4090 1213 828 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2330 2564 2959 3007 3080 4336 1353
Size: 977 234 395 48 73 1256 3423
Fragments arranged by size:
3423 1256 977 395 234 73 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4993 5367 5513 387
Size: 1064 138 3404 374 146 1280
Fragments arranged by size:
3404 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2437 2516 2870 2900 2940 3209 3364
Size: 393 805 79 354 30 40 269 155
Cuts at: 3364 3426 3668 4469 5066 5853 6121 1239
Size: 62 242 801 597 787 268 1524
Fragments arranged by size:
1524 805 801 787 597 393 354 269
268 242 155 79 62 40 30
EcoNI CCTnn'n_nnAGG
Cuts at: 3566 3602 3566
Size: 36 6370
EcoO109I rG'GnC_Cy
Cuts at: 2140 2548 2866 2987 3133 3559 2140
Size: 408 318 121 146 426 4987
Fragments arranged by size:
4987 426 408 318 146 121
EcoRI G'AATT_C
Cuts at: 3715 4093 3715
Size: 378 6028
FspI TGC'GCA
Cuts at: 2252 4912 2252
Size: 2660 3746
HaeII r_GCGC'y
Cuts at: 1678 2013 2314 2692 2732 2979 3318 3624 5553
Size: 335 301 378 40 247 339 306 1929
Cuts at: 5553 1678
Size: 2531
Fragments arranged by size:
2531 1929 378 339 335 306 301 247
40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5047 5079 5121 5153 1373
Size: 32 135 32 3475 32 42 32 2626
Fragments arranged by size:
3475 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3750 3996 669
Size: 988 281 1812 246 3079
Fragments arranged by size:
3079 1812 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6406
HpaI GTT'AAC
Cuts at: 3996 3996
Size: 6406
KasI G'GCGC_C
Cuts at: 2688 2728 2975 3314 2688
Size: 40 247 339 5780
Fragments arranged by size:
5780 339 247 40
KpnI G_GTAC'C
Cuts at: 2242 3731 2242
Size: 1489 4917
MfeI C'AATT_G
Cuts at: 4005 4005
Size: 6406
MluI A'CGCG_T
Cuts at: 6282 6282
Size: 6406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2178 2562 5520 5704 1305
Size: 873 384 2958 184 2007
Fragments arranged by size:
2958 2007 873 384 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2114 3374 10
Size: 54 1425 343 282 1260 3042
Fragments arranged by size:
3042 1425 1260 343 282 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2120 4364 4723 4882 518
Size: 648 954 2244 359 159 2042
Fragments arranged by size:
2244 2042 954 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2037 2046 2363 2940 3674 3822 4499 5647
Size: 431 9 317 577 734 148 677 1148
Cuts at: 5647 5892 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 734 677 577 431 317 245
148 9
NarI GG'CG_CC
Cuts at: 2689 2729 2976 3315 2689
Size: 40 247 339 5780
Fragments arranged by size:
5780 339 247 40
NcoI C'CATG_G
Cuts at: 513 1065 2886 513
Size: 552 1821 4033
Fragments arranged by size:
4033 1821 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6406
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6406
NotI GC'GGCC_GC
Cuts at: 3853 3853
Size: 6406
NspI r_CATG'y
Cuts at: 1132 1229 3764 5309 1132
Size: 97 2535 1545 2229
Fragments arranged by size:
2535 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6406
PciI A'CATG_T
Cuts at: 5305 5305
Size: 6406
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2283 2712 1108
Size: 1175 429 4802
Fragments arranged by size:
4802 1175 429
PfoI T'CCnGG_A
Cuts at: 2394 2394
Size: 6406
PmeI GTTT'AAAC
Cuts at: 3710 3710
Size: 6406
PmlI CAC'GTG
Cuts at: 2245 2245
Size: 6406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4475 4507 5988 6020 4475
Size: 32 1481 32 4861
Fragments arranged by size:
4861 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2866 2987 2866
Size: 121 6285
PshAI GACnn'nnGTC
Cuts at: 1613 2432 3417 1613
Size: 819 985 4602
Fragments arranged by size:
4602 985 819
PsiI TTA'TAA
Cuts at: 3976 3976
Size: 6406
PspOMI G'GGCC_C
Cuts at: 2081 2081
Size: 6406
PstI C_TGCA'G
Cuts at: 830 2139 2874 3758 830
Size: 1309 735 884 3478
Fragments arranged by size:
3478 1309 884 735
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6406
PvuII CAG'CTG
Cuts at: 2363 2363
Size: 6406
SalI G'TCGA_C
Cuts at: 1936 3748 1936
Size: 1812 4594
SapI GCTCTTCn'nnn_
Cuts at: 2564 2564
Size: 6406
SbfI CC_TGCA'GG
Cuts at: 3758 3758
Size: 6406
ScaI AGT'ACT
Cuts at: 4654 6366 4654
Size: 1712 4694
SfcI C'TryA_G
Cuts at: 826 1045 2135 2870 3415 3754 4889 5570 5761
Size: 219 1090 735 545 339 1135 681 191
Cuts at: 5761 826
Size: 1471
Fragments arranged by size:
1471 1135 1090 735 681 545 339 219
191
SfoI GGC'GCC
Cuts at: 2690 2730 2977 3316 2690
Size: 40 247 339 5780
Fragments arranged by size:
5780 339 247 40
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
SgrAI Cr'CCGG_yG
Cuts at: 1700 2119 1700
Size: 419 5987
SmaI CCC'GGG
Cuts at: 1872 2971 3018 3503 3733 1872
Size: 1099 47 485 230 4545
Fragments arranged by size:
4545 1099 485 230 47
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2920 3548 4510 5411 5673 820
Size: 197 926 977 628 962 901 262 1553
Fragments arranged by size:
1553 977 962 926 901 628 262 197
SnaBI TAC'GTA
Cuts at: 493 4197 493
Size: 3704 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6406
SphI G_CATG'C
Cuts at: 1132 3764 1132
Size: 2632 3774
SspI AAT'ATT
Cuts at: 5 52 4330 5
Size: 47 4278 2081
Fragments arranged by size:
4278 2081 47
StuI AGG'CCT
Cuts at: 1517 2072 1517
Size: 555 5851
StyI C'CwwG_G
Cuts at: 513 1065 2886 3845 513
Size: 552 1821 959 3074
Fragments arranged by size:
3074 1821 959 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2915 3238 4433 4450 4603 4788 5233
Size: 1255 1335 323 1195 17 153 185 445
Cuts at: 5233 325
Size: 1498
Fragments arranged by size:
1498 1335 1255 1195 445 323 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3645 4652 6364
Size: 274 80 33 51 1443 1668 1007 1712
Cuts at: 6364 96
Size: 138
Fragments arranged by size:
1712 1668 1443 1007 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2466 2498 6366 6398 2466
Size: 32 3868 32 2474
Fragments arranged by size:
3868 2474 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6406
XbaI T'CTAG_A
Cuts at: 3742 3742
Size: 6406
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6406
XmaI C'CCGG_G
Cuts at: 1870 2969 3016 3501 3731 1870
Size: 1099 47 485 230 4545
Fragments arranged by size:
4545 1099 485 230 47
XmnI GAAnn'nnTTC
Cuts at: 4535 4535
Size: 6406
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4214 276
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SrfI SwaI XcmI