Restriction map for
pFN21AB5979
(Circular) (Six-base) MAPSORT of: pFN21AB5979.seq Check: 3315 from: 1 to: 6265
pFN21AB5979 6265 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4075 278
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3608 1937
Size: 1671 4594
Acc65I G'GTAC_C
Cuts at: 3586 3586
Size: 6265
AclI AA'CG_TT
Cuts at: 1563 4392 4765 1563
Size: 2829 373 3063
Fragments arranged by size:
3063 2829 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2754 3303 4328 5712 5980 1632
Size: 1122 549 1025 1384 268 1917
Fragments arranged by size:
1917 1384 1122 1025 549 268
AfeI AGC'GCT
Cuts at: 2155 2155
Size: 6265
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6068
AflIII A'CryG_T
Cuts at: 1203 5164 6141 1203
Size: 3961 977 1327
Fragments arranged by size:
3961 1327 977
AgeI A'CCGG_T
Cuts at: 5096 5096
Size: 6265
AhdI GACnn_n'nnGTC
Cuts at: 4994 4994
Size: 6265
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3415 1184
Size: 2231 4034
AlwNI CAG_nnn'CTG
Cuts at: 4008 5580 4008
Size: 1572 4693
ApaI G_GGCC'C
Cuts at: 2998 2998
Size: 6265
ApaLI G'TGCA_C
Cuts at: 2675 4322 5478 2675
Size: 1647 1156 3462
Fragments arranged by size:
3462 1647 1156
ApoI r'AATT_y
Cuts at: 1493 3574 3800 3952 6061 1493
Size: 2081 226 152 2109 1697
Fragments arranged by size:
2109 2081 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4819 160
Size: 872 3787 1606
Fragments arranged by size:
3787 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
AvaI C'yCGr_G
Cuts at: 1870 1943 2019 2557 2670 3590 1870
Size: 73 76 538 113 920 4545
Fragments arranged by size:
4545 920 538 113 76 73
BamHI G'GATC_C
Cuts at: 2401 3595 3962 2401
Size: 1194 367 4704
Fragments arranged by size:
4704 1194 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2437 3586 5041 618
Size: 325 217 627 650 1149 1455 1842
Fragments arranged by size:
1842 1455 1149 650 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2018 2202 2998 3584 1391
Size: 627 184 796 586 4072
Fragments arranged by size:
4072 796 627 586 184
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3291 928
Size: 971 1392 3902
Fragments arranged by size:
3902 1392 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4456 4490 1903
Size: 34 2519 34 3678
Fragments arranged by size:
3678 2519 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3552 4157 5367 3552
Size: 605 1210 4450
Fragments arranged by size:
4450 1210 605
BclI T'GATC_A
Cuts at: 1548 2340 3295 1548
Size: 792 955 4518
Fragments arranged by size:
4518 955 792
BfrBI ATG'CAT
Cuts at: 2656 2656
Size: 6265
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4876 136
Size: 107 122 71 631 605 3204 1525
Fragments arranged by size:
3204 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6260 6260
Size: 6265
BlpI GC'TnA_GC
Cuts at: 3682 3682
Size: 6265
Bme1580I G_kGCm'C
Cuts at: 1790 2679 2998 4326 5482 1790
Size: 889 319 1328 1156 2573
Fragments arranged by size:
2573 1328 1156 889 319
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6265
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3996 4954 442
Size: 416 53 83 399 24 2579 958 1753
Fragments arranged by size:
2579 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2958 3274 4925 1239
Size: 1719 316 1651 2579
Fragments arranged by size:
2579 1719 1651 316
Bpu10I CC'TnA_GC
Cuts at: 2103 2103
Size: 6265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4390 5255 5553 4390
Size: 865 298 5102
Fragments arranged by size:
5102 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2050 2558 2885 4928 882
Size: 617 551 508 327 2043 2219
Fragments arranged by size:
2219 2043 617 551 508 327
BsaAI yAC'GTr
Cuts at: 493 1204 2680 4056 493
Size: 711 1476 1376 2702
Fragments arranged by size:
2702 1476 1376 711
BsaBI GATnn'nnATC
Cuts at: 3300 6259 3300
Size: 2959 3306
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4072 4454 5089 275
Size: 53 83 186 936 2539 382 635 1451
Fragments arranged by size:
2539 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4698 5096 5370 5517 5928 4698
Size: 398 274 147 411 5035
Fragments arranged by size:
5035 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6040
Fragments arranged by size:
6040 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2150 2153 1186
Size: 964 3 5298
Fragments arranged by size:
5298 964 3
BseYI C'CCAG_C
Cuts at: 2060 2495 5468 5964 2060
Size: 435 2973 496 2361
Fragments arranged by size:
2973 2361 496 435
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5692
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3715 4476 5504 1540
Size: 275 186 1714 761 1028 2301
Fragments arranged by size:
2301 1714 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2018 2679 4326 4411 5482 2018
Size: 661 1647 85 1071 2801
Fragments arranged by size:
2801 1647 1071 661 85
BsmI GAATG_Cn'
Cuts at: 3078 3776 3869 3078
Size: 698 93 5474
Fragments arranged by size:
5474 698 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2202 2679 2998 3584 4326 4411
Size: 399 228 184 477 319 586 742 85
Cuts at: 4411 5482 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 586 477 399 319 228
184 85
BspEI T'CCGG_A
Cuts at: 5928 5928
Size: 6265
BspHI T'CATG_A
Cuts at: 3222 4154 5884 3222
Size: 932 1730 3603
Fragments arranged by size:
3603 1730 932
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3102 3117 3620 844
Size: 2258 15 503 3489
Fragments arranged by size:
3489 2258 503 15
BsrBI CCG'CTC
Cuts at: 1394 1823 4152 1394
Size: 429 2329 3507
Fragments arranged by size:
3507 2329 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4760 4934 65
Size: 1183 3512 174 1396
Fragments arranged by size:
3512 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2440 3169 4909 5096 1700
Size: 110 630 729 1740 187 2869
Fragments arranged by size:
2869 1740 729 630 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6265
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6265
BssSI C'ACGA_G
Cuts at: 4325 5337 4325
Size: 1012 5253
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2653 794
Size: 1859 4406
BstBI TT'CG_AA
Cuts at: 5069 5069
Size: 6265
BstEII G'GTnAC_C
Cuts at: 1184 3256 1184
Size: 2072 4193
BstXI CCAn_nnnn'nTGG
Cuts at: 2434 2434
Size: 6265
BstYI r'GATC_y
Cuts at: 1972 2297 2401 3595 3962 4347 4364 5816 5902
Size: 325 104 1194 367 385 17 1452 86
Cuts at: 5902 5914 6260 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1194 385 367 346 325 104
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2037 2900 513
Size: 552 939 33 863 3878
Fragments arranged by size:
3878 939 863 552 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5526
Fragments arranged by size:
5526 683 56
BtsI GCAGTG_nn'
Cuts at: 2704 2968 3781 4575 4595 2704
Size: 264 813 794 20 4374
Fragments arranged by size:
4374 813 794 264 20
ClaI AT'CG_AT
Cuts at: 1554 2430 1554
Size: 876 5389
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6230
DraI TTT'AAA
Cuts at: 2758 2929 3569 2758
Size: 171 640 5454
Fragments arranged by size:
5454 640 171
DraIII CAC_nnn'GTG
Cuts at: 2954 2954
Size: 6265
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5272 809
Size: 507 3956 1802
Fragments arranged by size:
3956 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2424 3712 4601
Size: 54 1425 50 275 18 594 1288 889
Cuts at: 4601 6230 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1288 889 594 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3712 1537
Size: 275 1900 4090
Fragments arranged by size:
4090 1900 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2127 2159 2262 4195 1353
Size: 774 32 103 1933 3423
Fragments arranged by size:
3423 1933 774 103 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2108 2139 2377 4852 5226 5372
Size: 1064 138 519 31 238 2475 374 146
Cuts at: 5372 387
Size: 1280
Fragments arranged by size:
2475 1280 1064 519 374 238 146 138
31
EcoICRI GAG'CTC
Cuts at: 2016 2016
Size: 6265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2754 2958 3274 3303 4328 4925 5712
Size: 393 1122 204 316 29 1025 597 787
Cuts at: 5712 5980 1239
Size: 268 1524
Fragments arranged by size:
1524 1122 1025 787 597 393 316 268
204 29
EcoO109I rG'GnC_Cy
Cuts at: 2994 2995 2994
Size: 1 6264
EcoRI G'AATT_C
Cuts at: 3574 3952 3574
Size: 378 5887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2847 2847
Size: 6265
FspI TGC'GCA
Cuts at: 4771 4771
Size: 6265
HaeII r_GCGC'y
Cuts at: 1678 2157 5412 1678
Size: 479 3255 2531
Fragments arranged by size:
3255 2531 479
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4906 4938 4980 5012 1373
Size: 32 135 32 3334 32 42 32 2626
Fragments arranged by size:
3334 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2704 3106 3609 3855 669
Size: 988 281 766 402 503 246 3079
Fragments arranged by size:
3079 988 766 503 402 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6265
HpaI GTT'AAC
Cuts at: 3855 3855
Size: 6265
KpnI G_GTAC'C
Cuts at: 3590 3590
Size: 6265
MfeI C'AATT_G
Cuts at: 3864 3864
Size: 6265
MluI A'CGCG_T
Cuts at: 6141 6141
Size: 6265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2379 3158 3495 5379 5563 1305
Size: 1074 779 337 1884 184 2007
Fragments arranged by size:
2007 1884 1074 779 337 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2426 10
Size: 54 1425 343 594 3849
Fragments arranged by size:
3849 1425 594 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2432 4223 4582 4741 518
Size: 648 1266 1791 359 159 2042
Fragments arranged by size:
2042 1791 1266 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2238 3681 4358 5506 5751 1606
Size: 632 1443 677 1148 245 2120
Fragments arranged by size:
2120 1443 1148 677 632 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2900 513
Size: 552 939 896 3878
Fragments arranged by size:
3878 939 896 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6265
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6265
NotI GC'GGCC_GC
Cuts at: 3712 3712
Size: 6265
NsiI A_TGCA'T
Cuts at: 2658 2658
Size: 6265
NspI r_CATG'y
Cuts at: 1132 1229 3623 5168 1132
Size: 97 2394 1545 2229
Fragments arranged by size:
2394 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3250 1348
Size: 1902 4363
PciI A'CATG_T
Cuts at: 5164 5164
Size: 6265
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6265
PmeI GTTT'AAAC
Cuts at: 3569 3569
Size: 6265
PmlI CAC'GTG
Cuts at: 2680 2680
Size: 6265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4334 4366 5847 5879 4334
Size: 32 1481 32 4720
Fragments arranged by size:
4720 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3495 1613
Size: 1882 4383
PsiI TTA'TAA
Cuts at: 3835 3835
Size: 6265
PspOMI G'GGCC_C
Cuts at: 2994 2994
Size: 6265
PstI C_TGCA'G
Cuts at: 830 2161 3617 830
Size: 1331 1456 3478
Fragments arranged by size:
3478 1456 1331
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6265
SacI G_AGCT'C
Cuts at: 2018 2018
Size: 6265
SalI G'TCGA_C
Cuts at: 1936 3607 1936
Size: 1671 4594
SapI GCTCTTCn'nnn_
Cuts at: 2159 2159
Size: 6265
SbfI CC_TGCA'GG
Cuts at: 3617 3617
Size: 6265
ScaI AGT'ACT
Cuts at: 4513 6225 4513
Size: 1712 4553
SfcI C'TryA_G
Cuts at: 826 1045 2157 2458 3613 4748 5429 5620 826
Size: 219 1112 301 1155 1135 681 191 1471
Fragments arranged by size:
1471 1155 1135 1112 681 301 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6265
SmaI CCC'GGG
Cuts at: 1872 3592 1872
Size: 1720 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4369 5270 5532 820
Size: 197 926 2426 901 262 1553
Fragments arranged by size:
2426 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4056 493
Size: 3563 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6265
SphI G_CATG'C
Cuts at: 1132 3623 1132
Size: 2491 3774
SspI AAT'ATT
Cuts at: 5 52 3361 4189 5
Size: 47 3309 828 2081
Fragments arranged by size:
3309 2081 828 47
StuI AGG'CCT
Cuts at: 1517 2908 1517
Size: 1391 4874
StyI C'CwwG_G
Cuts at: 513 1065 2004 2046 2900 3067 3704 513
Size: 552 939 42 854 167 637 3074
Fragments arranged by size:
3074 939 854 637 552 167 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4292 4309 4462 4647 5092 325
Size: 1255 2712 17 153 185 445 1498
Fragments arranged by size:
2712 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2661 4511 6223
Size: 274 80 33 51 1443 684 1850 1712
Cuts at: 6223 96
Size: 138
Fragments arranged by size:
1850 1712 1443 684 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6225 6257 6225
Size: 32 6233
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6265
XbaI T'CTAG_A
Cuts at: 3452 3601 3452
Size: 149 6116
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2617 3141 2617
Size: 524 5741
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6265
XmaI C'CCGG_G
Cuts at: 1870 3590 1870
Size: 1720 4545
XmnI GAAnn'nnTTC
Cuts at: 2315 4394 2315
Size: 2079 4186
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4073 276
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PstI PvuI SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI
PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI