Restriction map for
pFN21AB5974
(Circular) (Six-base) MAPSORT of: pFN21AB5974.seq Check: 1901 from: 1 to: 6385
pFN21AB5974 6385 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4195 278
Size: 53 83 186 936 2659 2468
Fragments arranged by size:
2659 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3185 3728 1937
Size: 1248 543 4594
Fragments arranged by size:
4594 1248 543
Acc65I G'GTAC_C
Cuts at: 3706 3706
Size: 6385
AclI AA'CG_TT
Cuts at: 1563 4512 4885 1563
Size: 2949 373 3063
Fragments arranged by size:
3063 2949 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2066 2292 2308 2998 3099 4448 5832 6100
Size: 434 226 16 690 101 1349 1384 268
Cuts at: 6100 1632
Size: 1917
Fragments arranged by size:
1917 1384 1349 690 434 268 226 101
16
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6188
AflIII A'CryG_T
Cuts at: 1203 5284 6261 1203
Size: 4081 977 1327
Fragments arranged by size:
4081 1327 977
AgeI A'CCGG_T
Cuts at: 5216 5216
Size: 6385
AhdI GACnn_n'nnGTC
Cuts at: 5114 5114
Size: 6385
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6385
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6385
AlwNI CAG_nnn'CTG
Cuts at: 3602 4128 5700 3602
Size: 526 1572 4287
Fragments arranged by size:
4287 1572 526
ApaLI G'TGCA_C
Cuts at: 3633 4442 5598 3633
Size: 809 1156 4420
Fragments arranged by size:
4420 1156 809
ApoI r'AATT_y
Cuts at: 1493 2240 2704 3694 3920 4072 6181 1493
Size: 747 464 990 226 152 2109 1697
Fragments arranged by size:
2109 1697 990 747 464 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4939 160
Size: 872 3907 1606
Fragments arranged by size:
3907 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6385
AvaI C'yCGr_G
Cuts at: 1870 1943 3710 1870
Size: 73 1767 4545
Fragments arranged by size:
4545 1767 73
BamHI G'GATC_C
Cuts at: 2329 3715 4082 2329
Size: 1386 367 4632
Fragments arranged by size:
4632 1386 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3706 5161 618
Size: 325 217 627 1919 1455 1842
Fragments arranged by size:
1919 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3704 1391
Size: 2313 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3440 928
Size: 971 1541 3873
Fragments arranged by size:
3873 1541 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4576 4610 1903
Size: 34 2639 34 3678
Fragments arranged by size:
3678 2639 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4277 5487 4277
Size: 1210 5175
BclI T'GATC_A
Cuts at: 1548 3034 1548
Size: 1486 4899
BfrBI ATG'CAT
Cuts at: 2426 2738 2426
Size: 312 6073
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4996 136
Size: 107 122 71 631 605 337 2987 1525
Fragments arranged by size:
2987 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2303 6380 2303
Size: 4077 2308
BlpI GC'TnA_GC
Cuts at: 3802 3802
Size: 6385
Bme1580I G_kGCm'C
Cuts at: 1790 2534 3637 4446 5602 1790
Size: 744 1103 809 1156 2573
Fragments arranged by size:
2573 1156 1103 809 744
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6385
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2543 3090 4116
Size: 416 53 83 399 24 1126 547 1026
Cuts at: 4116 5074 442
Size: 958 1753
Fragments arranged by size:
1753 1126 1026 958 547 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3207 1056
Size: 2151 4234
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2619 2863 3157 3624 5045 1239
Size: 1380 244 294 467 1421 2579
Fragments arranged by size:
2579 1421 1380 467 294 244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2550 4510 5375 5673 2550
Size: 1960 865 298 3262
Fragments arranged by size:
3262 1960 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5048 882
Size: 617 3549 2219
Fragments arranged by size:
3549 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4176 493
Size: 711 2972 2702
Fragments arranged by size:
2972 2702 711
BsaBI GATnn'nnATC
Cuts at: 6379 6379
Size: 6385
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4192 4574 5209 275
Size: 53 83 186 936 2659 382 635 1451
Fragments arranged by size:
2659 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4818 5216 5490 5637 6048 4818
Size: 398 274 147 411 5155
Fragments arranged by size:
5155 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3421 3451 1178
Size: 30 165 30 2018 30 4112
Fragments arranged by size:
4112 2018 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 2631 3387 1186
Size: 862 583 756 4184
Fragments arranged by size:
4184 862 756 583
BseYI C'CCAG_C
Cuts at: 5588 6084 5588
Size: 496 5889
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2490 2501 3228 1163
Size: 573 754 11 727 4320
Fragments arranged by size:
4320 754 727 573 11
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2136 3835 4596 5624 1540
Size: 275 186 135 1699 761 1028 2301
Fragments arranged by size:
2301 1699 1028 761 275 186 135
BsiHKAI G_wGCw'C
Cuts at: 3637 4446 4531 5602 3637
Size: 809 85 1071 4420
Fragments arranged by size:
4420 1071 809 85
BsmI GAATG_Cn'
Cuts at: 3896 3989 3896
Size: 93 6292
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2534 3637 3704 4446 4531 5602 1391
Size: 399 744 1103 67 742 85 1071 2174
Fragments arranged by size:
2174 1103 1071 744 742 399 85 67
BspEI T'CCGG_A
Cuts at: 6048 6048
Size: 6385
BspHI T'CATG_A
Cuts at: 4274 6004 4274
Size: 1730 4655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3740 844
Size: 2896 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4272 1394
Size: 429 2449 3507
Fragments arranged by size:
3507 2449 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3217 4880 5054 65
Size: 1183 1969 1663 174 1396
Fragments arranged by size:
1969 1663 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2066 5029 5216 1700
Size: 110 256 2963 187 2869
Fragments arranged by size:
2963 2869 256 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6385
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6385
BssSI C'ACGA_G
Cuts at: 4445 5457 4445
Size: 1012 5373
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2723 2993 794
Size: 1929 270 4186
Fragments arranged by size:
4186 1929 270
BstBI TT'CG_AA
Cuts at: 5189 5189
Size: 6385
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6385
BstXI CCAn_nnnn'nTGG
Cuts at: 2410 2410
Size: 6385
BstYI r'GATC_y
Cuts at: 1972 2303 2329 3715 4082 4467 4484 5936 6022
Size: 331 26 1386 367 385 17 1452 86
Cuts at: 6022 6034 6380 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1386 385 367 346 331 86
26 17 12
BstZ17I GTA'TAC
Cuts at: 3186 3186
Size: 6385
Bsu36I CC'TnA_GG
Cuts at: 2469 2469
Size: 6385
BtgI C'CryG_G
Cuts at: 513 1065 2004 3417 513
Size: 552 939 1413 3481
Fragments arranged by size:
3481 1413 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5646
Fragments arranged by size:
5646 683 56
BtsI GCAGTG_nn'
Cuts at: 2513 3396 3901 4695 4715 2513
Size: 883 505 794 20 4183
Fragments arranged by size:
4183 883 794 505 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6385
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6350
DraI TTT'AAA
Cuts at: 3689 3689
Size: 6385
DraIII CAC_nnn'GTG
Cuts at: 2197 2197
Size: 6385
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5392 809
Size: 507 4076 1802
Fragments arranged by size:
4076 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 3832 4721
Size: 54 1425 50 275 18 180 1822 889
Cuts at: 4721 6350 8
Size: 1629 43
Fragments arranged by size:
1822 1629 1425 889 275 180 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3832 1537
Size: 275 2020 4090
Fragments arranged by size:
4090 2020 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4315 1353
Size: 2962 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4972 5346 5492 387
Size: 1064 138 3383 374 146 1280
Fragments arranged by size:
3383 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2292 2308 2619 2863 2998 3099
Size: 393 434 226 16 311 244 135 101
Cuts at: 3099 3157 3624 4448 5045 5832 6100 1239
Size: 58 467 824 597 787 268 1524
Fragments arranged by size:
1524 824 787 597 467 434 393 311
268 244 226 135 101 58 16
EcoO109I rG'GnC_Cy
Cuts at: 2439 2439
Size: 6385
EcoRI G'AATT_C
Cuts at: 2240 2704 3694 4072 2240
Size: 464 990 378 4553
Fragments arranged by size:
4553 990 464 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3129 3129
Size: 6385
FspI TGC'GCA
Cuts at: 4891 4891
Size: 6385
HaeII r_GCGC'y
Cuts at: 1678 5532 1678
Size: 3854 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2288 2320 2370 2402 2600
Size: 32 135 32 716 32 50 32 198
Cuts at: 2600 2632 5026 5058 5100 5132 1373
Size: 32 2394 32 42 32 2626
Fragments arranged by size:
2626 2394 716 198 135 50 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2125 2649 3729 3975 669
Size: 988 281 187 524 1080 246 3079
Fragments arranged by size:
3079 1080 988 524 281 246 187
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6385
HpaI GTT'AAC
Cuts at: 2125 3975 2125
Size: 1850 4535
KpnI G_GTAC'C
Cuts at: 3710 3710
Size: 6385
MfeI C'AATT_G
Cuts at: 3984 3984
Size: 6385
MluI A'CGCG_T
Cuts at: 6261 6261
Size: 6385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3677 5499 5683 1305
Size: 2372 1822 184 2007
Fragments arranged by size:
2372 2007 1822 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4563
Fragments arranged by size:
4563 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2259 3410 4343 4702 4861 518
Size: 648 1093 1151 933 359 159 2042
Fragments arranged by size:
2042 1151 1093 933 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2933 3383 3599 3801 4478 5626 5871 1606
Size: 1327 450 216 202 677 1148 245 2120
Fragments arranged by size:
2120 1327 1148 677 450 245 216 202
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4894
Fragments arranged by size:
4894 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6385
NheI G'CTAG_C
Cuts at: 1052 3203 1052
Size: 2151 4234
NotI GC'GGCC_GC
Cuts at: 3832 3832
Size: 6385
NsiI A_TGCA'T
Cuts at: 2428 2740 2428
Size: 312 6073
NspI r_CATG'y
Cuts at: 1132 1229 3415 3743 5288 1132
Size: 97 2186 328 1545 2229
Fragments arranged by size:
2229 2186 1545 328 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6385
PciI A'CATG_T
Cuts at: 5284 5284
Size: 6385
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6385
PmeI GTTT'AAAC
Cuts at: 3689 3689
Size: 6385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4454 4486 5967 5999 4454
Size: 32 1481 32 4840
Fragments arranged by size:
4840 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2439 2439
Size: 6385
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6385
PsiI TTA'TAA
Cuts at: 3955 3955
Size: 6385
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2887 2919 2887
Size: 32 6353
PstI C_TGCA'G
Cuts at: 830 2977 3166 3737 830
Size: 2147 189 571 3478
Fragments arranged by size:
3478 2147 571 189
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6385
PvuII CAG'CTG
Cuts at: 2933 3599 2933
Size: 666 5719
SalI G'TCGA_C
Cuts at: 1936 3727 1936
Size: 1791 4594
SbfI CC_TGCA'GG
Cuts at: 3737 3737
Size: 6385
ScaI AGT'ACT
Cuts at: 4633 6345 4633
Size: 1712 4673
SfcI C'TryA_G
Cuts at: 826 1045 2655 2814 2973 3162 3442 3733 4868
Size: 219 1610 159 159 189 280 291 1135
Cuts at: 4868 5549 5740 826
Size: 681 191 1471
Fragments arranged by size:
1610 1471 1135 681 291 280 219 191
189 159 159
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6385
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6385
SmaI CCC'GGG
Cuts at: 1872 3712 1872
Size: 1840 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2565 4489 5390 5652 820
Size: 197 926 622 1924 901 262 1553
Fragments arranged by size:
1924 1553 926 901 622 262 197
SnaBI TAC'GTA
Cuts at: 493 4176 493
Size: 3683 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6385
SphI G_CATG'C
Cuts at: 1132 3415 3743 1132
Size: 2283 328 3774
Fragments arranged by size:
3774 2283 328
SspI AAT'ATT
Cuts at: 5 52 4309 5
Size: 47 4257 2081
Fragments arranged by size:
4257 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6385
StyI C'CwwG_G
Cuts at: 513 1065 2004 3824 513
Size: 552 939 1820 3074
Fragments arranged by size:
3074 1820 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4412 4429 4582 4767 5212 325
Size: 1255 2832 17 153 185 445 1498
Fragments arranged by size:
2832 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4631 6343 96
Size: 274 80 33 51 1443 2654 1712 138
Fragments arranged by size:
2654 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3421 3453 6345 6377 3421
Size: 32 2892 32 3429
Fragments arranged by size:
3429 2892 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6385
XbaI T'CTAG_A
Cuts at: 3721 3721
Size: 6385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2816 2816
Size: 6385
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6385
XmaI C'CCGG_G
Cuts at: 1870 3710 1870
Size: 1840 4545
XmnI GAAnn'nnTTC
Cuts at: 4514 4514
Size: 6385
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4193 276
Size: 53 83 186 936 2659 2468
Fragments arranged by size:
2659 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI