Restriction map for
pFN21AB5973
(Circular) (Six-base) MAPSORT of: pFN21AB5973.seq Check: 982 from: 1 to: 6262
pFN21AB5973 6262 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4072 278
Size: 53 83 186 936 2536 2468
Fragments arranged by size:
2536 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2145 2157 3605 1937
Size: 208 12 1448 4594
Fragments arranged by size:
4594 1448 208 12
Acc65I G'GTAC_C
Cuts at: 3583 3583
Size: 6262
AclI AA'CG_TT
Cuts at: 1563 4389 4762 1563
Size: 2826 373 3063
Fragments arranged by size:
3063 2826 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2849 4325 5709 5977 1632
Size: 1217 1476 1384 268 1917
Fragments arranged by size:
1917 1476 1384 1217 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6065
AflIII A'CryG_T
Cuts at: 1203 5161 6138 1203
Size: 3958 977 1327
Fragments arranged by size:
3958 1327 977
AgeI A'CCGG_T
Cuts at: 5093 5093
Size: 6262
AhdI GACnn_n'nnGTC
Cuts at: 4991 4991
Size: 6262
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6262
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6262
AlwNI CAG_nnn'CTG
Cuts at: 2360 3315 4005 5577 2360
Size: 955 690 1572 3045
Fragments arranged by size:
3045 1572 955 690
ApaI G_GGCC'C
Cuts at: 2121 2974 2121
Size: 853 5409
ApaLI G'TGCA_C
Cuts at: 4319 5475 4319
Size: 1156 5106
ApoI r'AATT_y
Cuts at: 1493 3571 3797 3949 6058 1493
Size: 2078 226 152 2109 1697
Fragments arranged by size:
2109 2078 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4816 160
Size: 872 3784 1606
Fragments arranged by size:
3784 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
AvaI C'yCGr_G
Cuts at: 1870 1943 3404 3587 1870
Size: 73 1461 183 4545
Fragments arranged by size:
4545 1461 183 73
BamHI G'GATC_C
Cuts at: 3592 3959 3592
Size: 367 5895
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2137 2350 2598 3269 3282
Size: 325 217 627 350 213 248 671 13
Cuts at: 3282 3389 3506 3583 5038 618
Size: 107 117 77 1455 1842
Fragments arranged by size:
1842 1455 671 627 350 325 248 217
213 117 107 77 13
BanII G_rGCy'C
Cuts at: 1391 2121 2196 2718 2729 2745 2974 3581 1391
Size: 730 75 522 11 16 229 607 4072
Fragments arranged by size:
4072 730 607 522 229 75 16 11
BbeI G_GCGC'C
Cuts at: 2141 2602 3273 3393 3510 2141
Size: 461 671 120 117 4893
Fragments arranged by size:
4893 671 461 120 117
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3322 928
Size: 971 1423 3868
Fragments arranged by size:
3868 1423 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4453 4487 1903
Size: 34 2516 34 3678
Fragments arranged by size:
3678 2516 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2018 4154 5364 2018
Size: 2136 1210 2916
Fragments arranged by size:
2916 2136 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6262
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2129 3215 4873
Size: 107 122 71 631 605 457 1086 1658
Cuts at: 4873 136
Size: 1525
Fragments arranged by size:
1658 1525 1086 631 605 457 122 107
71
BglII A'GATC_T
Cuts at: 6257 6257
Size: 6262
BlpI GC'TnA_GC
Cuts at: 3096 3679 3096
Size: 583 5679
Bme1580I G_kGCm'C
Cuts at: 1790 2121 2974 3486 4323 5479 1790
Size: 331 853 512 837 1156 2573
Fragments arranged by size:
2573 1156 853 837 512 331
BmgBI CAC'GTC
Cuts at: 1318 2625 3240 1318
Size: 1307 615 4340
Fragments arranged by size:
4340 1307 615
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2041 3325 3993
Size: 416 53 83 399 24 624 1284 668
Cuts at: 3993 4951 442
Size: 958 1753
Fragments arranged by size:
1753 1284 958 668 624 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6262
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 6262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2789 2882 2984 3110 4922 1239
Size: 1550 93 102 126 1812 2579
Fragments arranged by size:
2579 1812 1550 126 102 93
Bpu10I CC'TnA_GC
Cuts at: 2072 3158 2072
Size: 1086 5176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2459 2588 2705 3173 4387 5252 5550 2459
Size: 129 117 468 1214 865 298 3171
Fragments arranged by size:
3171 1214 865 468 298 129 117
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2453 2575 4925 882
Size: 617 954 122 2350 2219
Fragments arranged by size:
2350 2219 954 617 122
BsaAI yAC'GTr
Cuts at: 493 1204 2079 2263 4053 493
Size: 711 875 184 1790 2702
Fragments arranged by size:
2702 1790 875 711 184
BsaBI GATnn'nnATC
Cuts at: 6256 6256
Size: 6262
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2138 2217 2282 2599
Size: 53 83 186 936 605 79 65 317
Cuts at: 2599 3077 3270 3390 3492 3507 4069 4451 5086
Size: 478 193 120 102 15 562 382 635
Cuts at: 5086 275
Size: 1451
Fragments arranged by size:
1451 936 635 605 562 478 382 317
193 186 120 102 83 79 65 53
15
BsaWI w'CCGG_w
Cuts at: 2090 2916 4695 5093 5367 5514 5925 2090
Size: 826 1779 398 274 147 411 2427
Fragments arranged by size:
2427 1779 826 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2018 2048 3275 3305 1178
Size: 30 165 30 615 30 1227 30 4135
Fragments arranged by size:
4135 1227 615 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2026 2693 2696 2699 2711 2714 2726 2771
Size: 840 667 3 3 12 3 12 45
Cuts at: 2771 1186
Size: 4677
Fragments arranged by size:
4677 840 667 45 12 12 3 3
3
BseYI C'CCAG_C
Cuts at: 2212 3055 3424 3496 5465 5961 2212
Size: 843 369 72 1969 496 2513
Fragments arranged by size:
2513 1969 843 496 369 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3435 1163
Size: 573 1699 3990
Fragments arranged by size:
3990 1699 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2205 3000 3712 4473 5501 1540
Size: 275 186 204 795 712 761 1028 2301
Fragments arranged by size:
2301 1028 795 761 712 275 204 186
BsiHKAI G_wGCw'C
Cuts at: 2078 2196 2496 2729 4323 4408 5479 2078
Size: 118 300 233 1594 85 1071 2861
Fragments arranged by size:
2861 1594 1071 300 233 118 85
BsmI GAATG_Cn'
Cuts at: 3773 3866 3773
Size: 93 6169
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 2121 2196 2496 2718 2729 2745
Size: 399 288 43 75 300 222 11 16
Cuts at: 2745 2974 3486 3581 4323 4408 5479 1391
Size: 229 512 95 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 512 399 300 288 229
222 95 85 75 43 16 11
BspEI T'CCGG_A
Cuts at: 2090 2916 5925 2090
Size: 826 3009 2427
Fragments arranged by size:
3009 2427 826
BspHI T'CATG_A
Cuts at: 4151 5881 4151
Size: 1730 4532
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3617 844
Size: 2773 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4149 1394
Size: 429 2326 3507
Fragments arranged by size:
3507 2326 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4757 4931 65
Size: 1183 3509 174 1396
Fragments arranged by size:
3509 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2140 2166 2595 2815 3067 3539 4906
Size: 110 330 26 429 220 252 472 1367
Cuts at: 4906 5093 1700
Size: 187 2869
Fragments arranged by size:
2869 1367 472 429 330 252 220 187
110 26
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6262
BssHII G'CGCG_C
Cuts at: 1925 2183 2591 2910 1925
Size: 258 408 319 5277
Fragments arranged by size:
5277 408 319 258
BssSI C'ACGA_G
Cuts at: 2189 4322 5334 2189
Size: 2133 1012 3117
Fragments arranged by size:
3117 2133 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2082 794
Size: 1288 4974
BstBI TT'CG_AA
Cuts at: 5066 5066
Size: 6262
BstEII G'GTnAC_C
Cuts at: 1184 3298 1184
Size: 2114 4148
BstYI r'GATC_y
Cuts at: 1972 2330 3592 3959 4344 4361 5813 5899 5911
Size: 358 1262 367 385 17 1452 86 12
Cuts at: 5911 6257 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1262 385 367 358 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2905 3036 3347 2905
Size: 131 311 5820
Fragments arranged by size:
5820 311 131
BtgI C'CryG_G
Cuts at: 513 1065 2205 2421 2633 2745 2931 3204 513
Size: 552 1140 216 212 112 186 273 3571
Fragments arranged by size:
3571 1140 552 273 216 212 186 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5523
Fragments arranged by size:
5523 683 56
BtsI GCAGTG_nn'
Cuts at: 3011 3778 4572 4592 3011
Size: 767 794 20 4681
Fragments arranged by size:
4681 794 767 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6262
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6227
DraI TTT'AAA
Cuts at: 3566 3566
Size: 6262
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2891 5269 809
Size: 507 1575 2378 1802
Fragments arranged by size:
2378 1802 1575 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2175 2202
Size: 54 1425 50 275 18 228 117 27
Cuts at: 2202 2538 2627 2928 2997 3709 4598 6227 8
Size: 336 89 301 69 712 889 1629 43
Fragments arranged by size:
1629 1425 889 712 336 301 275 228
117 89 69 54 50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2202 2997 3709 1537
Size: 275 390 795 712 4090
Fragments arranged by size:
4090 795 712 390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4192 1353
Size: 2839 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2123 2253 2275 2955 3462 4849
Size: 1064 138 534 130 22 680 507 1387
Cuts at: 4849 5223 5369 387
Size: 374 146 1280
Fragments arranged by size:
1387 1280 1064 680 534 507 374 146
138 130 22
EcoICRI GAG'CTC
Cuts at: 2194 2727 2194
Size: 533 5729
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2789 2849 2882 2984 3110 4325 4922
Size: 393 1157 60 33 102 126 1215 597
Cuts at: 4922 5709 5977 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1215 1157 787 597 393 268 126
102 60 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2407 2564 2606 3533 2407
Size: 157 42 927 5136
Fragments arranged by size:
5136 927 157 42
EcoO109I rG'GnC_Cy
Cuts at: 2228 2344 2959 2970 3200 2228
Size: 116 615 11 230 5290
Fragments arranged by size:
5290 615 230 116 11
EcoRI G'AATT_C
Cuts at: 3571 3949 3571
Size: 378 5884
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2854 2854
Size: 6262
FspI TGC'GCA
Cuts at: 2337 2647 4768 2337
Size: 310 2121 3831
Fragments arranged by size:
3831 2121 310
HaeII r_GCGC'y
Cuts at: 1678 2141 2213 2602 3196 3273 3393 3510 5409
Size: 463 72 389 594 77 120 117 1899
Cuts at: 5409 1678
Size: 2531
Fragments arranged by size:
2531 1899 594 463 389 120 117 77
72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3161 3193 4903 4935 4977
Size: 32 135 32 1589 32 1710 32 42
Cuts at: 4977 5009 1373
Size: 32 2626
Fragments arranged by size:
2626 1710 1589 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3606 3852 669
Size: 988 281 1668 246 3079
Fragments arranged by size:
3079 1668 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6262
HpaI GTT'AAC
Cuts at: 3852 3852
Size: 6262
KasI G'GCGC_C
Cuts at: 2137 2598 3269 3389 3506 2137
Size: 461 671 120 117 4893
Fragments arranged by size:
4893 671 461 120 117
KpnI G_GTAC'C
Cuts at: 3587 3587
Size: 6262
MfeI C'AATT_G
Cuts at: 3861 3861
Size: 6262
MluI A'CGCG_T
Cuts at: 6138 6138
Size: 6262
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5376 5560 1305
Size: 4071 184 2007
Fragments arranged by size:
4071 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2060 2177 2540 10
Size: 54 1425 343 228 117 363 3732
Fragments arranged by size:
3732 1425 363 343 228 117 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2552 4220 4579 4738 518
Size: 648 1386 1668 359 159 2042
Fragments arranged by size:
2042 1668 1386 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2207 2360 2430 2635 2800 2933 3137 3206
Size: 601 153 70 205 165 133 204 69
Cuts at: 3206 3500 3678 4355 5503 5748 1606
Size: 294 178 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 601 294 245 205 204
178 165 153 133 70 69
NaeI GCC'GGC
Cuts at: 2168 2597 2817 3069 3541 2168
Size: 429 220 252 472 4889
Fragments arranged by size:
4889 472 429 252 220
NarI GG'CG_CC
Cuts at: 2138 2599 3270 3390 3507 2138
Size: 461 671 120 117 4893
Fragments arranged by size:
4893 671 461 120 117
NcoI C'CATG_G
Cuts at: 513 1065 2421 513
Size: 552 1356 4354
Fragments arranged by size:
4354 1356 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6262
NgoMIV G'CCGG_C
Cuts at: 2166 2595 2815 3067 3539 2166
Size: 429 220 252 472 4889
Fragments arranged by size:
4889 472 429 252 220
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6262
NotI GC'GGCC_GC
Cuts at: 3709 3709
Size: 6262
NspI r_CATG'y
Cuts at: 1132 1229 3187 3620 5165 1132
Size: 97 1958 433 1545 2229
Fragments arranged by size:
2229 1958 1545 433 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6262
PciI A'CATG_T
Cuts at: 5161 5161
Size: 6262
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2848 1108
Size: 1740 4522
PfoI T'CCnGG_A
Cuts at: 3396 3396
Size: 6262
PmeI GTTT'AAAC
Cuts at: 3566 3566
Size: 6262
PmlI CAC'GTG
Cuts at: 2079 2079
Size: 6262
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4331 4363 5844 5876 4331
Size: 32 1481 32 4717
Fragments arranged by size:
4717 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2228 2959 3200 2228
Size: 731 241 5290
Fragments arranged by size:
5290 731 241
PshAI GACnn'nnGTC
Cuts at: 1613 2856 1613
Size: 1243 5019
PsiI TTA'TAA
Cuts at: 3832 3832
Size: 6262
PspOMI G'GGCC_C
Cuts at: 2117 2970 2117
Size: 853 5409
PstI C_TGCA'G
Cuts at: 830 2490 3505 3614 830
Size: 1660 1015 109 3478
Fragments arranged by size:
3478 1660 1015 109
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6262
PvuII CAG'CTG
Cuts at: 3500 3500
Size: 6262
SacI G_AGCT'C
Cuts at: 2196 2729 2196
Size: 533 5729
SacII CC_GC'GG
Cuts at: 2208 2636 2934 3207 2208
Size: 428 298 273 5263
Fragments arranged by size:
5263 428 298 273
SalI G'TCGA_C
Cuts at: 1936 3604 1936
Size: 1668 4594
SanDI GG'GwC_CC
Cuts at: 2228 2228
Size: 6262
SbfI CC_TGCA'GG
Cuts at: 3614 3614
Size: 6262
ScaI AGT'ACT
Cuts at: 4510 6222 4510
Size: 1712 4550
SexAI A'CCwGG_T
Cuts at: 2576 2576
Size: 6262
SfcI C'TryA_G
Cuts at: 826 1045 2486 3501 3610 4745 5426 5617 826
Size: 219 1441 1015 109 1135 681 191 1471
Fragments arranged by size:
1471 1441 1135 1015 681 219 191 109
SfoI GGC'GCC
Cuts at: 2139 2600 3271 3391 3508 2139
Size: 461 671 120 117 4893
Fragments arranged by size:
4893 671 461 120 117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
SgrAI Cr'CCGG_yG
Cuts at: 1700 2595 2815 1700
Size: 895 220 5147
Fragments arranged by size:
5147 895 220
SmaI CCC'GGG
Cuts at: 1872 3406 3589 1872
Size: 1534 183 4545
Fragments arranged by size:
4545 1534 183
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2474 2603 2720 3188 4366 5267
Size: 197 926 531 129 117 468 1178 901
Cuts at: 5267 5529 820
Size: 262 1553
Fragments arranged by size:
1553 1178 926 901 531 468 262 197
129 117
SnaBI TAC'GTA
Cuts at: 493 4053 493
Size: 3560 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6262
SphI G_CATG'C
Cuts at: 1132 3187 3620 1132
Size: 2055 433 3774
Fragments arranged by size:
3774 2055 433
SspI AAT'ATT
Cuts at: 5 52 4186 5
Size: 47 4134 2081
Fragments arranged by size:
4134 2081 47
StuI AGG'CCT
Cuts at: 1517 2480 2903 3087 3371 1517
Size: 963 423 184 284 4408
Fragments arranged by size:
4408 963 423 284 184
StyI C'CwwG_G
Cuts at: 513 1065 2316 2421 2517 3196 3384 3534 3701
Size: 552 1251 105 96 679 188 150 167
Cuts at: 3701 513
Size: 3074
Fragments arranged by size:
3074 1251 679 552 188 167 150 105
96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3307 4289 4306 4459 4644 5089 325
Size: 1255 1727 982 17 153 185 445 1498
Fragments arranged by size:
1727 1498 1255 982 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4508 6220 96
Size: 274 80 33 51 1443 2531 1712 138
Fragments arranged by size:
2531 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3275 3307 6222 6254 3275
Size: 32 2915 32 3283
Fragments arranged by size:
3283 2915 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3326 1316
Size: 2010 4252
XbaI T'CTAG_A
Cuts at: 3598 3598
Size: 6262
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6262
XmaI C'CCGG_G
Cuts at: 1870 3404 3587 1870
Size: 1534 183 4545
Fragments arranged by size:
4545 1534 183
XmnI GAAnn'nnTTC
Cuts at: 4391 4391
Size: 6262
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4070 276
Size: 53 83 186 936 2536 2468
Fragments arranged by size:
2536 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI
NruI NsiI PacI PspXI PsrI RsrII SapI SfiI
SrfI SwaI XcmI