Restriction map for
pFN21AB5968
(Circular) (Six-base) MAPSORT of: pFN21AB5968.seq Check: 3531 from: 1 to: 6373
pFN21AB5968 6373 bp
With 182 enzymes: SgfI *
January 30, 2014 17:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2114 2128 2114
Size: 14 6359
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3273 4183 278
Size: 53 83 186 936 1737 910 2468
Fragments arranged by size:
2468 1737 936 910 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3527 3716 1937
Size: 1590 189 4594
Fragments arranged by size:
4594 1590 189
Acc65I G'GTAC_C
Cuts at: 3694 3694
Size: 6373
AclI AA'CG_TT
Cuts at: 1563 4500 4873 1563
Size: 2937 373 3063
Fragments arranged by size:
3063 2937 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2175 3372 3624 4436 5820 6088 1632
Size: 543 1197 252 812 1384 268 1917
Fragments arranged by size:
1917 1384 1197 812 543 268 252
AflII C'TTAA_G
Cuts at: 820 1017 3532 820
Size: 197 2515 3661
Fragments arranged by size:
3661 2515 197
AflIII A'CryG_T
Cuts at: 1203 2369 3466 5272 6249 1203
Size: 1166 1097 1806 977 1327
Fragments arranged by size:
1806 1327 1166 1097 977
AgeI A'CCGG_T
Cuts at: 5204 5204
Size: 6373
AhdI GACnn_n'nnGTC
Cuts at: 5102 5102
Size: 6373
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6373
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2430 3087 1184
Size: 1246 657 4470
Fragments arranged by size:
4470 1246 657
AlwNI CAG_nnn'CTG
Cuts at: 4116 5688 4116
Size: 1572 4801
ApaI G_GGCC'C
Cuts at: 2020 2020
Size: 6373
ApaLI G'TGCA_C
Cuts at: 4430 5586 4430
Size: 1156 5217
ApoI r'AATT_y
Cuts at: 1493 2534 3317 3447 3644 3682 3908 4060 6169
Size: 1041 783 130 197 38 226 152 2109
Cuts at: 6169 1493
Size: 1697
Fragments arranged by size:
2109 1697 1041 783 226 197 152 130
38
AseI AT'TA_AT
Cuts at: 160 1032 4927 160
Size: 872 3895 1606
Fragments arranged by size:
3895 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6373
AvaI C'yCGr_G
Cuts at: 1870 1943 2520 3698 1870
Size: 73 577 1178 4545
Fragments arranged by size:
4545 1178 577 73
BamHI G'GATC_C
Cuts at: 3703 4070 3703
Size: 367 6006
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3694 5149 618
Size: 325 217 627 1907 1455 1842
Fragments arranged by size:
1907 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2020 2988 3692 1391
Size: 629 968 704 4072
Fragments arranged by size:
4072 968 704 629
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2072 2181 3265 3300 928
Size: 971 173 109 1084 35 4001
Fragments arranged by size:
4001 1084 971 173 109 35
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4564 4598 1903
Size: 34 2627 34 3678
Fragments arranged by size:
3678 2627 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2192 2282 4265 5475 2192
Size: 90 1983 1210 3090
Fragments arranged by size:
3090 1983 1210 90
BclI T'GATC_A
Cuts at: 1548 3154 1548
Size: 1606 4767
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4984 136
Size: 107 122 71 631 605 3312 1525
Fragments arranged by size:
3312 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2325 6368 2325
Size: 4043 2330
BlpI GC'TnA_GC
Cuts at: 2640 3790 2640
Size: 1150 5223
Bme1580I G_kGCm'C
Cuts at: 1790 2020 2724 4434 5590 1790
Size: 230 704 1710 1156 2573
Fragments arranged by size:
2573 1710 1156 704 230
BmgBI CAC'GTC
Cuts at: 1318 3469 1318
Size: 2151 4222
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4104 5062 442
Size: 416 53 83 399 24 2687 958 1753
Fragments arranged by size:
2687 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3458 1056
Size: 2402 3971
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3002 3002
Size: 6373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2251 2652 2972 5033 1239
Size: 1012 401 320 2061 2579
Fragments arranged by size:
2579 2061 1012 401 320
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2995 4498 5363 5661 2995
Size: 1503 865 298 3707
Fragments arranged by size:
3707 1503 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5036 882
Size: 617 3537 2219
Fragments arranged by size:
3537 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4164 493
Size: 711 2960 2702
Fragments arranged by size:
2960 2702 711
BsaBI GATnn'nnATC
Cuts at: 6367 6367
Size: 6373
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2217 2651 3270 4180
Size: 53 83 186 936 684 434 619 910
Cuts at: 4180 4562 5197 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 910 684 635 619 434 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2592 4806 5204 5478 5625 6036 2592
Size: 2214 398 274 147 411 2929
Fragments arranged by size:
2929 2214 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3044 3074 1178
Size: 30 165 30 1641 30 4477
Fragments arranged by size:
4477 1641 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6373
BseYI C'CCAG_C
Cuts at: 5576 6072 5576
Size: 496 5877
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5800
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3823 4584 5612 1540
Size: 275 186 1822 761 1028 2301
Fragments arranged by size:
2301 1822 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2988 4434 4519 5590 2988
Size: 1446 85 1071 3771
Fragments arranged by size:
3771 1446 1071 85
BsmI GAATG_Cn'
Cuts at: 2302 3884 3977 2302
Size: 1582 93 4698
Fragments arranged by size:
4698 1582 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2020 2724 2988 3692 4434 4519 5590
Size: 399 230 704 264 704 742 85 1071
Cuts at: 5590 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 704 704 399 264 230
85
BspEI T'CCGG_A
Cuts at: 6036 6036
Size: 6373
BspHI T'CATG_A
Cuts at: 4262 5992 4262
Size: 1730 4643
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2114 2128 2972 3167 3728 844
Size: 1270 14 844 195 561 3489
Fragments arranged by size:
3489 1270 844 561 195 14
BsrBI CCG'CTC
Cuts at: 1394 1823 4260 1394
Size: 429 2437 3507
Fragments arranged by size:
3507 2437 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2419 3663 4868 5042 65
Size: 1183 1171 1244 1205 174 1396
Fragments arranged by size:
1396 1244 1205 1183 1171 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5017 5204 1700
Size: 110 3207 187 2869
Fragments arranged by size:
3207 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6373
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6373
BssSI C'ACGA_G
Cuts at: 4433 5445 4433
Size: 1012 5361
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6373
BstBI TT'CG_AA
Cuts at: 2083 5177 2083
Size: 3094 3279
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6373
BstYI r'GATC_y
Cuts at: 1972 2325 3703 4070 4455 4472 5924 6010 6022
Size: 353 1378 367 385 17 1452 86 12
Cuts at: 6022 6368 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1378 385 367 353 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2730 3283 2730
Size: 553 5820
BtgI C'CryG_G
Cuts at: 513 1065 2057 2910 513
Size: 552 992 853 3976
Fragments arranged by size:
3976 992 853 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5634
Fragments arranged by size:
5634 683 56
BtsI GCAGTG_nn'
Cuts at: 3889 4683 4703 3889
Size: 794 20 5559
Fragments arranged by size:
5559 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6373
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2311 2346 529
Size: 35 1747 35 4556
Fragments arranged by size:
4556 1747 35 35
DraI TTT'AAA
Cuts at: 3577 3677 3577
Size: 100 6273
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2753 5380 809
Size: 507 1437 2627 1802
Fragments arranged by size:
2627 1802 1437 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2539 2670 3820
Size: 54 1425 50 275 18 709 131 1150
Cuts at: 3820 4709 6338 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1150 889 709 275 131 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3820 1537
Size: 275 2008 4090
Fragments arranged by size:
4090 2008 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2976 3465 4303 1353
Size: 1623 489 838 3423
Fragments arranged by size:
3423 1623 838 489
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2740 4960 5334 5480 387
Size: 1064 138 1151 2220 374 146 1280
Fragments arranged by size:
2220 1280 1151 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2986 2986
Size: 6373
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2175 2251 2652 2972 3372 3624 4436
Size: 393 543 76 401 320 400 252 812
Cuts at: 4436 5033 5820 6088 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 812 787 597 543 401 400 393
320 268 252 76
EcoO109I rG'GnC_Cy
Cuts at: 2016 2017 2016
Size: 1 6372
EcoRI G'AATT_C
Cuts at: 3682 4060 3682
Size: 378 5995
EcoRV GAT'ATC
Cuts at: 2809 2809
Size: 6373
FspI TGC'GCA
Cuts at: 4879 4879
Size: 6373
HaeII r_GCGC'y
Cuts at: 1678 5520 1678
Size: 3842 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2327 2359 5014 5046 5088
Size: 32 135 32 755 32 2655 32 42
Cuts at: 5088 5120 1373
Size: 32 2626
Fragments arranged by size:
2655 2626 755 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3717 3963 669
Size: 988 281 1779 246 3079
Fragments arranged by size:
3079 1779 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6373
HpaI GTT'AAC
Cuts at: 3963 3963
Size: 6373
KpnI G_GTAC'C
Cuts at: 3698 3698
Size: 6373
MfeI C'AATT_G
Cuts at: 3065 3972 3065
Size: 907 5466
MluI A'CGCG_T
Cuts at: 6249 6249
Size: 6373
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5487 5671 1305
Size: 4182 184 2007
Fragments arranged by size:
4182 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4551
Fragments arranged by size:
4551 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2358 4331 4690 4849 518
Size: 648 1192 1973 359 159 2042
Fragments arranged by size:
2042 1973 1192 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2475 2647 3049 3789 4466 5614 5859 1606
Size: 869 172 402 740 677 1148 245 2120
Fragments arranged by size:
2120 1148 869 740 677 402 245 172
NcoI C'CATG_G
Cuts at: 513 1065 2910 513
Size: 552 1845 3976
Fragments arranged by size:
3976 1845 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6373
NheI G'CTAG_C
Cuts at: 1052 3454 1052
Size: 2402 3971
NotI GC'GGCC_GC
Cuts at: 3820 3820
Size: 6373
NspI r_CATG'y
Cuts at: 1132 1229 2373 3731 5276 1132
Size: 97 1144 1358 1545 2229
Fragments arranged by size:
2229 1545 1358 1144 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6373
PciI A'CATG_T
Cuts at: 2369 5272 2369
Size: 2903 3470
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6373
PfoI T'CCnGG_A
Cuts at: 2228 2228
Size: 6373
PmeI GTTT'AAAC
Cuts at: 3677 3677
Size: 6373
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3319 3351 4442 4474 5955 5987 3319
Size: 32 1091 32 1481 32 3705
Fragments arranged by size:
3705 1481 1091 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6373
PsiI TTA'TAA
Cuts at: 3943 3943
Size: 6373
PspOMI G'GGCC_C
Cuts at: 2016 2016
Size: 6373
PstI C_TGCA'G
Cuts at: 830 2125 3725 830
Size: 1295 1600 3478
Fragments arranged by size:
3478 1600 1295
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6373
SacI G_AGCT'C
Cuts at: 2988 2988
Size: 6373
SalI G'TCGA_C
Cuts at: 1936 3715 1936
Size: 1779 4594
SapI GCTCTTCn'nnn_
Cuts at: 2976 3465 2976
Size: 489 5884
SbfI CC_TGCA'GG
Cuts at: 2125 3725 2125
Size: 1600 4773
ScaI AGT'ACT
Cuts at: 4621 6333 4621
Size: 1712 4661
SfcI C'TryA_G
Cuts at: 826 1045 2121 3473 3721 4856 5537 5728 826
Size: 219 1076 1352 248 1135 681 191 1471
Fragments arranged by size:
1471 1352 1135 1076 681 248 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6373
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6373
SmaI CCC'GGG
Cuts at: 1872 3700 1872
Size: 1828 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2520 2974 3532 4477 5378 5640
Size: 197 926 577 454 558 945 901 262
Cuts at: 5640 820
Size: 1553
Fragments arranged by size:
1553 945 926 901 577 558 454 262
197
SnaBI TAC'GTA
Cuts at: 493 4164 493
Size: 3671 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6373
SphI G_CATG'C
Cuts at: 1132 3731 1132
Size: 2599 3774
SspI AAT'ATT
Cuts at: 5 52 4297 5
Size: 47 4245 2081
Fragments arranged by size:
4245 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6373
StyI C'CwwG_G
Cuts at: 513 1065 2910 3812 513
Size: 552 1845 902 3074
Fragments arranged by size:
3074 1845 902 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2128 4400 4417 4570 4755 5200 325
Size: 1255 548 2272 17 153 185 445 1498
Fragments arranged by size:
2272 1498 1255 548 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2278 2581 3240
Size: 274 80 33 51 1443 301 303 659
Cuts at: 3240 3382 4619 6331 96
Size: 142 1237 1712 138
Fragments arranged by size:
1712 1443 1237 659 303 301 274 142
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3044 3076 6333 6365 3044
Size: 32 3257 32 3052
Fragments arranged by size:
3257 3052 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6373
XbaI T'CTAG_A
Cuts at: 3184 3709 3184
Size: 525 5848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2627 2627
Size: 6373
XhoI C'TCGA_G
Cuts at: 1943 2520 1943
Size: 577 5796
XmaI C'CCGG_G
Cuts at: 1870 3698 1870
Size: 1828 4545
XmnI GAAnn'nnTTC
Cuts at: 2313 4502 2313
Size: 2189 4184
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3271 4181 276
Size: 53 83 186 936 1737 910 2468
Fragments arranged by size:
2468 1737 936 910 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI