Restriction map for
pFN21AB5961
(Circular) (Six-base) MAPSORT of: pFN21AB5961.seq Check: 1132 from: 1 to: 6250
pFN21AB5961 6250 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4060 278
Size: 53 83 186 936 2524 2468
Fragments arranged by size:
2524 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3593 1937
Size: 1656 4594
Acc65I G'GTAC_C
Cuts at: 3571 3571
Size: 6250
AclI AA'CG_TT
Cuts at: 1563 4377 4750 1563
Size: 2814 373 3063
Fragments arranged by size:
3063 2814 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3057 4313 5697 5965 1632
Size: 1425 1256 1384 268 1917
Fragments arranged by size:
1917 1425 1384 1256 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6053
AflIII A'CryG_T
Cuts at: 1203 5149 6126 1203
Size: 3946 977 1327
Fragments arranged by size:
3946 1327 977
AgeI A'CCGG_T
Cuts at: 5081 5081
Size: 6250
AhdI GACnn_n'nnGTC
Cuts at: 4979 4979
Size: 6250
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6250
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2042 3126 1184
Size: 858 1084 4308
Fragments arranged by size:
4308 1084 858
AlwNI CAG_nnn'CTG
Cuts at: 3993 5565 3993
Size: 1572 4678
ApaLI G'TGCA_C
Cuts at: 4307 5463 4307
Size: 1156 5094
ApoI r'AATT_y
Cuts at: 1493 3559 3785 3937 6046 1493
Size: 2066 226 152 2109 1697
Fragments arranged by size:
2109 2066 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4804 160
Size: 872 3772 1606
Fragments arranged by size:
3772 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6250
AvaI C'yCGr_G
Cuts at: 1870 1943 3575 1870
Size: 73 1632 4545
Fragments arranged by size:
4545 1632 73
BamHI G'GATC_C
Cuts at: 3580 3947 3580
Size: 367 5883
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 3571 5026 618
Size: 325 217 627 282 1502 1455 1842
Fragments arranged by size:
1842 1502 1455 627 325 282 217
BanII G_rGCy'C
Cuts at: 1391 2087 3569 1391
Size: 696 1482 4072
Fragments arranged by size:
4072 1482 696
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5279
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4441 4475 1903
Size: 34 2504 34 3678
Fragments arranged by size:
3678 2504 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4142 5352 4142
Size: 1210 5040
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6250
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2060 2558 4861
Size: 107 122 71 631 605 388 498 2303
Cuts at: 4861 136
Size: 1525
Fragments arranged by size:
2303 1525 631 605 498 388 122 107
71
BglII A'GATC_T
Cuts at: 6245 6245
Size: 6250
BlpI GC'TnA_GC
Cuts at: 3667 3667
Size: 6250
Bme1580I G_kGCm'C
Cuts at: 1790 4311 5467 1790
Size: 2521 1156 2573
Fragments arranged by size:
2573 2521 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6250
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2532 3981 4939
Size: 416 53 83 399 24 1115 1449 958
Cuts at: 4939 442
Size: 1753
Fragments arranged by size:
1753 1449 1115 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2127 2474 4910 1239
Size: 888 347 2436 2579
Fragments arranged by size:
2579 2436 888 347
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3135 4375 5240 5538 3135
Size: 1240 865 298 3847
Fragments arranged by size:
3847 1240 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4913 882
Size: 617 3414 2219
Fragments arranged by size:
3414 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4041 493
Size: 711 2837 2702
Fragments arranged by size:
2837 2702 711
BsaBI GATnn'nnATC
Cuts at: 6244 6244
Size: 6250
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4057 4439 5074 275
Size: 53 83 186 936 2524 382 635 1451
Fragments arranged by size:
2524 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4683 5081 5355 5502 5913 4683
Size: 398 274 147 411 5020
Fragments arranged by size:
5020 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6025
Fragments arranged by size:
6025 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2234 2237 1186
Size: 1048 3 5199
Fragments arranged by size:
5199 1048 3
BseYI C'CCAG_C
Cuts at: 2055 5453 5949 2055
Size: 3398 496 2356
Fragments arranged by size:
3398 2356 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5677
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3700 4461 5489 1540
Size: 275 186 1699 761 1028 2301
Fragments arranged by size:
2301 1699 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2347 4311 4396 5467 2347
Size: 1964 85 1071 3130
Fragments arranged by size:
3130 1964 1071 85
BsmI GAATG_Cn'
Cuts at: 3761 3854 3761
Size: 93 6157
BsmBI CGTCTCn'nnnn_
Cuts at: 2103 2198 2103
Size: 95 6155
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2347 3569 4311 4396 5467 1391
Size: 399 297 260 1222 742 85 1071 2174
Fragments arranged by size:
2174 1222 1071 742 399 297 260 85
BspEI T'CCGG_A
Cuts at: 5913 5913
Size: 6250
BspHI T'CATG_A
Cuts at: 2772 4139 5869 2772
Size: 1367 1730 3153
Fragments arranged by size:
3153 1730 1367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2482 3605 844
Size: 1638 1123 3489
Fragments arranged by size:
3489 1638 1123
BsrBI CCG'CTC
Cuts at: 1394 1823 4137 1394
Size: 429 2314 3507
Fragments arranged by size:
3507 2314 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4745 4919 65
Size: 1183 3497 174 1396
Fragments arranged by size:
3497 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4894 5081 1700
Size: 110 3084 187 2869
Fragments arranged by size:
3084 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6250
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6250
BssSI C'ACGA_G
Cuts at: 4310 5322 4310
Size: 1012 5238
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6250
BstBI TT'CG_AA
Cuts at: 5054 5054
Size: 6250
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6250
BstYI r'GATC_y
Cuts at: 1972 2882 3329 3580 3947 4332 4349 5801 5887
Size: 910 447 251 367 385 17 1452 86
Cuts at: 5887 5899 6245 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 910 447 385 367 346 251
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2125 513
Size: 552 939 121 4638
Fragments arranged by size:
4638 939 552 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5511
Fragments arranged by size:
5511 683 56
BtsI GCAGTG_nn'
Cuts at: 2486 2606 2832 3110 3766 4560 4580 2486
Size: 120 226 278 656 794 20 4156
Fragments arranged by size:
4156 794 656 278 226 120 20
ClaI AT'CG_AT
Cuts at: 1554 3283 1554
Size: 1729 4521
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6215
DraI TTT'AAA
Cuts at: 2752 2889 3554 2752
Size: 137 665 5448
Fragments arranged by size:
5448 665 137
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5257 809
Size: 507 3941 1802
Fragments arranged by size:
3941 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3697 4586 6215
Size: 54 1425 50 275 18 1867 889 1629
Cuts at: 6215 8
Size: 43
Fragments arranged by size:
1867 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3697 1537
Size: 275 1885 4090
Fragments arranged by size:
4090 1885 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3091 4180 1353
Size: 1738 1089 3423
Fragments arranged by size:
3423 1738 1089
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4837 5211 5357 387
Size: 1064 138 3248 374 146 1280
Fragments arranged by size:
3248 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 2474 3057 4313 4910 5697 5965
Size: 393 495 347 583 1256 597 787 268
Cuts at: 5965 1239
Size: 1524
Fragments arranged by size:
1524 1256 787 597 583 495 393 347
268
EcoO109I rG'GnC_Cy
Cuts at: 2175 2667 3146 2175
Size: 492 479 5279
Fragments arranged by size:
5279 492 479
EcoRI G'AATT_C
Cuts at: 3559 3937 3559
Size: 378 5872
FspI TGC'GCA
Cuts at: 4756 4756
Size: 6250
HaeII r_GCGC'y
Cuts at: 1678 5397 1678
Size: 3719 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2329 2361 2508 2540 4891
Size: 32 135 32 757 32 147 32 2351
Cuts at: 4891 4923 4965 4997 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2351 757 147 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3594 3840 669
Size: 988 281 1656 246 3079
Fragments arranged by size:
3079 1656 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3203 748
Size: 2455 3795
HpaI GTT'AAC
Cuts at: 3840 3840
Size: 6250
KpnI G_GTAC'C
Cuts at: 3575 3575
Size: 6250
MfeI C'AATT_G
Cuts at: 3849 3849
Size: 6250
MluI A'CGCG_T
Cuts at: 6126 6126
Size: 6250
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2562 3215 5364 5548 1305
Size: 1257 653 2149 184 2007
Fragments arranged by size:
2149 2007 1257 653 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4428
Fragments arranged by size:
4428 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2736 4208 4567 4726 518
Size: 648 1570 1472 359 159 2042
Fragments arranged by size:
2042 1570 1472 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2172 2242 3666 4343 5491 5736 1606
Size: 566 70 1424 677 1148 245 2120
Fragments arranged by size:
2120 1424 1148 677 566 245 70
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4759
Fragments arranged by size:
4759 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6250
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6250
NotI GC'GGCC_GC
Cuts at: 3697 3697
Size: 6250
NspI r_CATG'y
Cuts at: 1132 1229 3608 5153 1132
Size: 97 2379 1545 2229
Fragments arranged by size:
2379 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6250
PciI A'CATG_T
Cuts at: 5149 5149
Size: 6250
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6250
PmeI GTTT'AAAC
Cuts at: 3554 3554
Size: 6250
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4319 4351 5832 5864 4319
Size: 32 1481 32 4705
Fragments arranged by size:
4705 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2175 2667 2175
Size: 492 5758
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6250
PsiI TTA'TAA
Cuts at: 3820 3820
Size: 6250
PstI C_TGCA'G
Cuts at: 830 2493 2580 3602 830
Size: 1663 87 1022 3478
Fragments arranged by size:
3478 1663 1022 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6250
SalI G'TCGA_C
Cuts at: 1936 3592 1936
Size: 1656 4594
SbfI CC_TGCA'GG
Cuts at: 3602 3602
Size: 6250
ScaI AGT'ACT
Cuts at: 4498 6210 4498
Size: 1712 4538
SfcI C'TryA_G
Cuts at: 826 1045 2489 2576 3063 3598 4733 5414 5605
Size: 219 1444 87 487 535 1135 681 191
Cuts at: 5605 826
Size: 1471
Fragments arranged by size:
1471 1444 1135 681 535 487 219 191
87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6250
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6250
SmaI CCC'GGG
Cuts at: 1872 3577 1872
Size: 1705 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3150 4354 5255 5517 820
Size: 197 926 1207 1204 901 262 1553
Fragments arranged by size:
1553 1207 1204 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4041 493
Size: 3548 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6250
SphI G_CATG'C
Cuts at: 1132 3608 1132
Size: 2476 3774
SspI AAT'ATT
Cuts at: 5 52 2443 4174 5
Size: 47 2391 1731 2081
Fragments arranged by size:
2391 2081 1731 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6250
StyI C'CwwG_G
Cuts at: 513 1065 2004 3689 513
Size: 552 939 1685 3074
Fragments arranged by size:
3074 1685 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4277 4294 4447 4632 5077 325
Size: 1255 2697 17 153 185 445 1498
Fragments arranged by size:
2697 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2279 4496 6208
Size: 274 80 33 51 1443 302 2217 1712
Cuts at: 6208 96
Size: 138
Fragments arranged by size:
2217 1712 1443 302 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6210 6242 6210
Size: 32 6218
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6250
XbaI T'CTAG_A
Cuts at: 3586 3586
Size: 6250
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6250
XmaI C'CCGG_G
Cuts at: 1870 3575 1870
Size: 1705 4545
XmnI GAAnn'nnTTC
Cuts at: 2709 4379 2709
Size: 1670 4580
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4058 276
Size: 53 83 186 936 2524 2468
Fragments arranged by size:
2524 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI