Restriction map for
pFN21AB5957
(Circular) (Six-base) MAPSORT of: pFN21AB5957.seq Check: 6622 from: 1 to: 6244
pFN21AB5957 6244 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2057 2057
Size: 6244
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2485 4054 278
Size: 53 83 186 936 949 1569 2468
Fragments arranged by size:
2468 1569 949 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3587 1937
Size: 1650 4594
Acc65I G'GTAC_C
Cuts at: 3565 3565
Size: 6244
AclI AA'CG_TT
Cuts at: 1563 4371 4744 1563
Size: 2808 373 3063
Fragments arranged by size:
3063 2808 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2333 3409 4307 5691 5959 1632
Size: 701 1076 898 1384 268 1917
Fragments arranged by size:
1917 1384 1076 898 701 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6047
AflIII A'CryG_T
Cuts at: 1203 2154 5143 6120 1203
Size: 951 2989 977 1327
Fragments arranged by size:
2989 1327 977 951
AgeI A'CCGG_T
Cuts at: 5075 5075
Size: 6244
AhdI GACnn_n'nnGTC
Cuts at: 2871 4973 2871
Size: 2102 4142
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6244
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6244
AlwNI CAG_nnn'CTG
Cuts at: 3043 3987 5559 3043
Size: 944 1572 3728
Fragments arranged by size:
3728 1572 944
ApaI G_GGCC'C
Cuts at: 2271 2491 2271
Size: 220 6024
ApaLI G'TGCA_C
Cuts at: 4301 5457 4301
Size: 1156 5088
ApoI r'AATT_y
Cuts at: 1493 3553 3779 3931 6040 1493
Size: 2060 226 152 2109 1697
Fragments arranged by size:
2109 2060 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4798 160
Size: 872 3766 1606
Fragments arranged by size:
3766 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
AvaI C'yCGr_G
Cuts at: 1870 1943 2399 3056 3439 3569 1870
Size: 73 456 657 383 130 4545
Fragments arranged by size:
4545 657 456 383 130 73
BamHI G'GATC_C
Cuts at: 3574 3941 3574
Size: 367 5877
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2211 2654 3565 5020 618
Size: 325 217 627 424 443 911 1455 1842
Fragments arranged by size:
1842 1455 911 627 443 424 325 217
BanII G_rGCy'C
Cuts at: 1391 2082 2271 2310 2491 2745 3092 3262 3563
Size: 691 189 39 181 254 347 170 301
Cuts at: 3563 1391
Size: 4072
Fragments arranged by size:
4072 691 347 301 254 189 181 170
39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3177 928
Size: 971 1278 3995
Fragments arranged by size:
3995 1278 971
BbvCI CC'TCA_GC
Cuts at: 2690 3202 2690
Size: 512 5732
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2579 2613 2683 2717 4435 4469 1903
Size: 34 642 34 70 34 1718 34 3678
Fragments arranged by size:
3678 1718 642 70 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4136 5346 4136
Size: 1210 5034
BclI T'GATC_A
Cuts at: 1548 3465 1548
Size: 1917 4327
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2209 2266 3415
Size: 107 122 71 631 605 537 57 1149
Cuts at: 3415 4855 136
Size: 1440 1525
Fragments arranged by size:
1525 1440 1149 631 605 537 122 107
71 57
BglII A'GATC_T
Cuts at: 6239 6239
Size: 6244
BlpI GC'TnA_GC
Cuts at: 2706 3661 2706
Size: 955 5289
Bme1580I G_kGCm'C
Cuts at: 1790 2141 2165 2271 2491 4305 5461 1790
Size: 351 24 106 220 1814 1156 2573
Fragments arranged by size:
2573 1814 1156 351 220 106 24
BmgBI CAC'GTC
Cuts at: 1318 2956 1318
Size: 1638 4606
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2659 3975 4933
Size: 416 53 83 399 24 1242 1316 958
Cuts at: 4933 442
Size: 1753
Fragments arranged by size:
1753 1316 1242 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6244
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2531 2531
Size: 6244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2799 3287 4904 1239
Size: 1560 488 1617 2579
Fragments arranged by size:
2579 1617 1560 488
Bpu10I CC'TnA_GC
Cuts at: 2690 3202 2690
Size: 512 5732
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2539 2636 4369 5234 5532 2539
Size: 97 1733 865 298 3251
Fragments arranged by size:
3251 1733 865 298 97
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2036 2171 3349 3527 4907 882
Size: 617 537 135 1178 178 1380 2219
Fragments arranged by size:
2219 1380 1178 617 537 178 135
BsaAI yAC'GTr
Cuts at: 493 1204 4035 493
Size: 711 2831 2702
Fragments arranged by size:
2831 2702 711
BsaBI GATnn'nnATC
Cuts at: 2342 6238 2342
Size: 3896 2348
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2482 4051 4433 5068
Size: 53 83 186 936 949 1569 382 635
Cuts at: 5068 275
Size: 1451
Fragments arranged by size:
1569 1451 949 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4677 5075 5349 5496 5907 4677
Size: 398 274 147 411 5014
Fragments arranged by size:
5014 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2579 2609 1178
Size: 30 165 30 1176 30 4813
Fragments arranged by size:
4813 1176 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2342 2417 2633 3450 1186
Size: 1156 75 216 817 3980
Fragments arranged by size:
3980 1156 817 216 75
BseYI C'CCAG_C
Cuts at: 2133 2984 5447 5943 2133
Size: 851 2463 496 2434
Fragments arranged by size:
2463 2434 851 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3202 3474 1163
Size: 573 1466 272 3933
Fragments arranged by size:
3933 1466 573 272
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2261 2362 3694 4455 5483 1540
Size: 275 186 260 101 1332 761 1028 2301
Fragments arranged by size:
2301 1332 1028 761 275 260 186 101
BsiHKAI G_wGCw'C
Cuts at: 2376 2745 4305 4390 5461 2376
Size: 369 1560 85 1071 3159
Fragments arranged by size:
3159 1560 1071 369 85
BsmI GAATG_Cn'
Cuts at: 3755 3848 3755
Size: 93 6151
BsmBI CGTCTCn'nnnn_
Cuts at: 2473 2473
Size: 6244
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2141 2165 2271 2310 2376 2491
Size: 399 292 59 24 106 39 66 115
Cuts at: 2491 2745 3092 3262 3563 4305 4390 5461 1391
Size: 254 347 170 301 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 347 301 292 254
170 115 106 85 66 59 39 24
BspEI T'CCGG_A
Cuts at: 5907 5907
Size: 6244
BspHI T'CATG_A
Cuts at: 4133 5863 4133
Size: 1730 4514
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2057 3051 3599 844
Size: 1213 994 548 3489
Fragments arranged by size:
3489 1213 994 548
BsrBI CCG'CTC
Cuts at: 1394 1823 2257 2364 2398 3301 4131 1394
Size: 429 434 107 34 903 830 3507
Fragments arranged by size:
3507 903 830 434 429 107 34
BsrDI GCAATG_nn'
Cuts at: 65 1248 4739 4913 65
Size: 1183 3491 174 1396
Fragments arranged by size:
3491 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2208 4888 5075 1700
Size: 110 398 2680 187 2869
Fragments arranged by size:
2869 2680 398 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6244
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6244
BssSI C'ACGA_G
Cuts at: 2505 4304 5316 2505
Size: 1799 1012 3433
Fragments arranged by size:
3433 1799 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6244
BstBI TT'CG_AA
Cuts at: 5048 5048
Size: 6244
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6244
BstXI CCAn_nnnn'nTGG
Cuts at: 2350 2350
Size: 6244
BstYI r'GATC_y
Cuts at: 1972 3574 3941 4326 4343 5795 5881 5893 6239
Size: 1602 367 385 17 1452 86 12 346
Cuts at: 6239 1972
Size: 1977
Fragments arranged by size:
1977 1602 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2377 3528 513
Size: 552 939 373 1151 3229
Fragments arranged by size:
3229 1151 939 552 373
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3088 487
Size: 683 56 1862 3643
Fragments arranged by size:
3643 1862 683 56
BtsI GCAGTG_nn'
Cuts at: 2174 3234 3760 4554 4574 2174
Size: 1060 526 794 20 3844
Fragments arranged by size:
3844 1060 794 526 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6244
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6209
DraI TTT'AAA
Cuts at: 2454 2472 3548 2454
Size: 18 1076 5150
Fragments arranged by size:
5150 1076 18
DraIII CAC_nnn'GTG
Cuts at: 2389 2389
Size: 6244
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2155 5251 809
Size: 507 839 3096 1802
Fragments arranged by size:
3096 1802 839 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2098 2217
Size: 54 1425 50 275 18 192 76 119
Cuts at: 2217 2258 2380 2631 3502 3691 4580 6209 8
Size: 41 122 251 871 189 889 1629 43
Fragments arranged by size:
1629 1425 889 871 275 251 192 189
122 119 76 54 50 43 41 18
EagI C'GGCC_G
Cuts at: 1537 1812 2258 3691 1537
Size: 275 446 1433 4090
Fragments arranged by size:
4090 1433 446 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2471 2709 4174 1353
Size: 1118 238 1465 3423
Fragments arranged by size:
3423 1465 1118 238
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4831 5205 5351 387
Size: 1064 138 3242 374 146 1280
Fragments arranged by size:
3242 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2743 2743
Size: 6244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2333 2799 3287 3409 4307 4904 5691
Size: 393 701 466 488 122 898 597 787
Cuts at: 5691 5959 1239
Size: 268 1524
Fragments arranged by size:
1524 898 787 701 597 488 466 393
268 122
EcoNI CCTnn'n_nnAGG
Cuts at: 2036 3035 2036
Size: 999 5245
EcoO109I rG'GnC_Cy
Cuts at: 2267 3040 2267
Size: 773 5471
EcoRI G'AATT_C
Cuts at: 3553 3931 3553
Size: 378 5866
FspI TGC'GCA
Cuts at: 2249 4750 2249
Size: 2501 3743
HaeII r_GCGC'y
Cuts at: 1678 5391 1678
Size: 3713 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2246 2278 2797 2829 2857
Size: 32 135 32 674 32 519 32 28
Cuts at: 2857 2889 3052 3084 4885 4917 4959 4991 1373
Size: 32 163 32 1801 32 42 32 2626
Fragments arranged by size:
2626 1801 674 519 163 135 42 32
32 32 32 32 32 32 32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 3588 3834 669
Size: 988 281 1650 246 3079
Fragments arranged by size:
3079 1650 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6244
HpaI GTT'AAC
Cuts at: 3834 3834
Size: 6244
KpnI G_GTAC'C
Cuts at: 3569 3569
Size: 6244
MfeI C'AATT_G
Cuts at: 3843 3843
Size: 6244
MluI A'CGCG_T
Cuts at: 6120 6120
Size: 6244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3058 5358 5542 1305
Size: 1753 2300 184 2007
Fragments arranged by size:
2300 2007 1753 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2219 2633 3504 10
Size: 54 1425 343 387 414 871 2750
Fragments arranged by size:
2750 1425 871 414 387 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2348 4202 4561 4720 518
Size: 648 1182 1854 359 159 2042
Fragments arranged by size:
2042 1854 1182 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2130 2245 2379 2569 3307 3660 4337 5485
Size: 524 115 134 190 738 353 677 1148
Cuts at: 5485 5730 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 738 677 524 353 245 190
134 115
NcoI C'CATG_G
Cuts at: 513 1065 2004 3528 513
Size: 552 939 1524 3229
Fragments arranged by size:
3229 1524 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6244
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6244
NotI GC'GGCC_GC
Cuts at: 3691 3691
Size: 6244
NspI r_CATG'y
Cuts at: 1132 1229 2200 3602 5147 1132
Size: 97 971 1402 1545 2229
Fragments arranged by size:
2229 1545 1402 971 97
PasI CC'CwG_GG
Cuts at: 1348 2491 1348
Size: 1143 5101
PciI A'CATG_T
Cuts at: 5143 5143
Size: 6244
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3310 1108
Size: 2202 4042
PfoI T'CCnGG_A
Cuts at: 2012 2012
Size: 6244
PmeI GTTT'AAAC
Cuts at: 3548 3548
Size: 6244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4313 4345 5826 5858 4313
Size: 32 1481 32 4699
Fragments arranged by size:
4699 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3040 3040
Size: 6244
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6244
PsiI TTA'TAA
Cuts at: 3814 3814
Size: 6244
PspOMI G'GGCC_C
Cuts at: 2267 2487 2267
Size: 220 6024
PspXI vC'TCGA_Gb
Cuts at: 2399 2399
Size: 6244
PstI C_TGCA'G
Cuts at: 830 2037 2067 2751 3596 830
Size: 1207 30 684 845 3478
Fragments arranged by size:
3478 1207 845 684 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6244
PvuII CAG'CTG
Cuts at: 2245 2569 2245
Size: 324 5920
SacI G_AGCT'C
Cuts at: 2745 2745
Size: 6244
SacII CC_GC'GG
Cuts at: 2380 2380
Size: 6244
SalI G'TCGA_C
Cuts at: 1936 3586 1936
Size: 1650 4594
SbfI CC_TGCA'GG
Cuts at: 2751 3596 2751
Size: 845 5399
ScaI AGT'ACT
Cuts at: 2069 4492 6204 2069
Size: 2423 1712 2109
Fragments arranged by size:
2423 2109 1712
SfcI C'TryA_G
Cuts at: 826 1045 2033 2063 2747 3592 4727 5408 5599
Size: 219 988 30 684 845 1135 681 191
Cuts at: 5599 826
Size: 1471
Fragments arranged by size:
1471 1135 988 845 684 681 219 191
30
SfiI GGCCn_nnn'nGGCC
Cuts at: 2266 3415 2266
Size: 1149 5095
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6244
SmaI CCC'GGG
Cuts at: 1872 3571 1872
Size: 1699 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2399 2518 2615 4348 5249 5511
Size: 197 926 456 119 97 1733 901 262
Cuts at: 5511 820
Size: 1553
Fragments arranged by size:
1733 1553 926 901 456 262 197 119
97
SnaBI TAC'GTA
Cuts at: 493 4035 493
Size: 3542 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6244
SphI G_CATG'C
Cuts at: 1132 3602 1132
Size: 2470 3774
SspI AAT'ATT
Cuts at: 5 52 3254 4168 5
Size: 47 3202 914 2081
Fragments arranged by size:
3202 2081 914 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6244
StyI C'CwwG_G
Cuts at: 513 1065 2004 2801 3528 3683 513
Size: 552 939 797 727 155 3074
Fragments arranged by size:
3074 939 797 727 552 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2678 4271 4288 4441 4626 5071 325
Size: 1255 1098 1593 17 153 185 445 1498
Fragments arranged by size:
1593 1498 1255 1098 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2067 2319 4490
Size: 274 80 33 51 1443 90 252 2171
Cuts at: 4490 6202 96
Size: 1712 138
Fragments arranged by size:
2171 1712 1443 274 252 138 90 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3145 3177 6204 6236 3145
Size: 32 3027 32 3153
Fragments arranged by size:
3153 3027 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6244
XbaI T'CTAG_A
Cuts at: 3580 3580
Size: 6244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2212 2212
Size: 6244
XhoI C'TCGA_G
Cuts at: 1943 2399 1943
Size: 456 5788
XmaI C'CCGG_G
Cuts at: 1870 3569 1870
Size: 1699 4545
XmnI GAAnn'nnTTC
Cuts at: 3218 4373 3218
Size: 1155 5089
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2483 4052 276
Size: 53 83 186 936 949 1569 2468
Fragments arranged by size:
2468 1569 949 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII
SanDI SapI SexAI SfoI SrfI SwaI