Restriction map for
pFN21AB5948
(Circular) (Six-base) MAPSORT of: pFN21AB5948.seq Check: 1201 from: 1 to: 6337
pFN21AB5948 6337 bp
With 182 enzymes: SgfI *
November 21, 2014 12:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4147 278
Size: 53 83 186 936 2611 2468
Fragments arranged by size:
2611 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3680 1937
Size: 1743 4594
Acc65I G'GTAC_C
Cuts at: 3658 3658
Size: 6337
AclI AA'CG_TT
Cuts at: 1563 4464 4837 1563
Size: 2901 373 3063
Fragments arranged by size:
3063 2901 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2064 2142 2208 2538 4400 5784 6052 1632
Size: 432 78 66 330 1862 1384 268 1917
Fragments arranged by size:
1917 1862 1384 432 330 268 78 66
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6140
AflIII A'CryG_T
Cuts at: 1203 5236 6213 1203
Size: 4033 977 1327
Fragments arranged by size:
4033 1327 977
AgeI A'CCGG_T
Cuts at: 5168 5168
Size: 6337
AhdI GACnn_n'nnGTC
Cuts at: 5066 5066
Size: 6337
AleI CACnn'nnGTG
Cuts at: 1166 3269 1166
Size: 2103 4234
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3121 1184
Size: 1937 4400
AlwNI CAG_nnn'CTG
Cuts at: 2489 2846 4080 5652 2489
Size: 357 1234 1572 3174
Fragments arranged by size:
3174 1572 1234 357
ApaLI G'TGCA_C
Cuts at: 2442 4394 5550 2442
Size: 1952 1156 3229
Fragments arranged by size:
3229 1952 1156
ApoI r'AATT_y
Cuts at: 1493 2273 2283 2375 2943 3646 3872 4024 6133
Size: 780 10 92 568 703 226 152 2109
Cuts at: 6133 1493
Size: 1697
Fragments arranged by size:
2109 1697 780 703 568 226 152 92
10
AseI AT'TA_AT
Cuts at: 160 1032 2930 2937 4891 160
Size: 872 1898 7 1954 1606
Fragments arranged by size:
1954 1898 1606 872 7
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6337
AvaI C'yCGr_G
Cuts at: 1870 1943 3188 3662 1870
Size: 73 1245 474 4545
Fragments arranged by size:
4545 1245 474 73
BamHI G'GATC_C
Cuts at: 3667 4034 3667
Size: 367 5970
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3292 3658 5113 618
Size: 325 217 627 1505 366 1455 1842
Fragments arranged by size:
1842 1505 1455 627 366 325 217
BanII G_rGCy'C
Cuts at: 1391 3450 3656 1391
Size: 2059 206 4072
Fragments arranged by size:
4072 2059 206
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5366
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4528 4562 1903
Size: 34 2591 34 3678
Fragments arranged by size:
3678 2591 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2444 4229 5439 2444
Size: 1785 1210 3342
Fragments arranged by size:
3342 1785 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6337
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4948 136
Size: 107 122 71 631 605 3276 1525
Fragments arranged by size:
3276 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2211 2754 6332 2211
Size: 543 3578 2216
Fragments arranged by size:
3578 2216 543
BlpI GC'TnA_GC
Cuts at: 3754 3754
Size: 6337
Bme1580I G_kGCm'C
Cuts at: 1790 2302 2446 3235 4398 5554 1790
Size: 512 144 789 1163 1156 2573
Fragments arranged by size:
2573 1163 1156 789 512 144
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6337
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4068 5026 442
Size: 416 53 83 399 24 2651 958 1753
Fragments arranged by size:
2651 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3426 3434 3565 4997 1239
Size: 2187 8 131 1432 2579
Fragments arranged by size:
2579 2187 1432 131 8
Bpu10I CC'TnA_GC
Cuts at: 3246 3246
Size: 6337
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3524 4462 5327 5625 3524
Size: 938 865 298 4236
Fragments arranged by size:
4236 938 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3065 3348 5000 882
Size: 617 1566 283 1652 2219
Fragments arranged by size:
2219 1652 1566 617 283
BsaAI yAC'GTr
Cuts at: 493 1204 4128 493
Size: 711 2924 2702
Fragments arranged by size:
2924 2702 711
BsaBI GATnn'nnATC
Cuts at: 6331 6331
Size: 6337
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4144 4526 5161 275
Size: 53 83 186 936 2611 382 635 1451
Fragments arranged by size:
2611 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4770 5168 5442 5589 6000 4770
Size: 398 274 147 411 5107
Fragments arranged by size:
5107 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2081 2111 2292 2322 1178
Size: 30 165 30 678 30 181 30 5193
Fragments arranged by size:
5193 678 181 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2034 2181 2765 3582 1186
Size: 848 147 584 817 3941
Fragments arranged by size:
3941 848 817 584 147
BseYI C'CCAG_C
Cuts at: 5540 6036 5540
Size: 496 5841
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3122 1163
Size: 573 1386 4378
Fragments arranged by size:
4378 1386 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3787 4548 5576 1540
Size: 275 186 1786 761 1028 2301
Fragments arranged by size:
2301 1786 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2446 3450 4398 4483 5554 2446
Size: 1004 948 85 1071 3229
Fragments arranged by size:
3229 1071 1004 948 85
BsmI GAATG_Cn'
Cuts at: 3848 3941 3848
Size: 93 6244
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2302 2446 3235 3450 3656 4398 4483
Size: 399 512 144 789 215 206 742 85
Cuts at: 4483 5554 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 789 742 512 399 215 206
144 85
BspEI T'CCGG_A
Cuts at: 6000 6000
Size: 6337
BspHI T'CATG_A
Cuts at: 2952 4226 5956 2952
Size: 1274 1730 3333
Fragments arranged by size:
3333 1730 1274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3692 844
Size: 2848 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4224 1394
Size: 429 2401 3507
Fragments arranged by size:
3507 2401 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2300 2631 2865 4832 5006 65
Size: 1183 1052 331 234 1967 174 1396
Fragments arranged by size:
1967 1396 1183 1052 331 234 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4981 5168 1700
Size: 110 3171 187 2869
Fragments arranged by size:
3171 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2703 96
Size: 2607 3730
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6337
BssSI C'ACGA_G
Cuts at: 4397 5409 4397
Size: 1012 5325
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3114 794
Size: 2320 4017
BstBI TT'CG_AA
Cuts at: 5141 5141
Size: 6337
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6337
BstXI CCAn_nnnn'nTGG
Cuts at: 2295 2295
Size: 6337
BstYI r'GATC_y
Cuts at: 1972 2211 2754 3527 3667 4034 4419 4436 5888
Size: 239 543 773 140 367 385 17 1452
Cuts at: 5888 5974 5986 6332 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 773 543 385 367 346 239
140 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3297 3297
Size: 6337
BtgI C'CryG_G
Cuts at: 513 1065 2112 513
Size: 552 1047 4738
Fragments arranged by size:
4738 1047 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5598
Fragments arranged by size:
5598 683 56
BtsI GCAGTG_nn'
Cuts at: 3853 4647 4667 3853
Size: 794 20 5523
Fragments arranged by size:
5523 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6337
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6302
DraI TTT'AAA
Cuts at: 3492 3561 3641 3492
Size: 69 80 6188
Fragments arranged by size:
6188 80 69
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5344 809
Size: 507 4028 1802
Fragments arranged by size:
4028 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2886 3784 4673
Size: 54 1425 50 275 18 1056 898 889
Cuts at: 4673 6302 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1056 898 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3784 1537
Size: 275 1972 4090
Fragments arranged by size:
4090 1972 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2022 2118 2223 2526 4267 1353
Size: 669 96 105 303 1741 3423
Fragments arranged by size:
3423 1741 669 303 105 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4924 5298 5444 387
Size: 1064 138 3335 374 146 1280
Fragments arranged by size:
3335 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3448 3448
Size: 6337
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2064 2142 2208 2538 3426 3434 3565
Size: 393 432 78 66 330 888 8 131
Cuts at: 3565 4400 4997 5784 6052 1239
Size: 835 597 787 268 1524
Fragments arranged by size:
1524 888 835 787 597 432 393 330
268 131 78 66 8
EcoO109I rG'GnC_Cy
Cuts at: 2791 2791
Size: 6337
EcoRI G'AATT_C
Cuts at: 2273 2283 3646 4024 2273
Size: 10 1363 378 4586
Fragments arranged by size:
4586 1363 378 10
EcoRV GAT'ATC
Cuts at: 3337 3337
Size: 6337
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2162 2162
Size: 6337
FspI TGC'GCA
Cuts at: 4843 4843
Size: 6337
HaeII r_GCGC'y
Cuts at: 1678 2206 5484 1678
Size: 528 3278 2531
Fragments arranged by size:
3278 2531 528
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3326 3358 3452 3484 4978
Size: 32 135 32 1754 32 94 32 1494
Cuts at: 4978 5010 5052 5084 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1754 1494 135 94 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2401 3681 3927 669
Size: 988 281 463 1280 246 3079
Fragments arranged by size:
3079 1280 988 463 281 246
HindIII A'AGCT_T
Cuts at: 748 3123 3315 748
Size: 2375 192 3770
Fragments arranged by size:
3770 2375 192
HpaI GTT'AAC
Cuts at: 3927 3927
Size: 6337
KpnI G_GTAC'C
Cuts at: 3662 3662
Size: 6337
MfeI C'AATT_G
Cuts at: 3360 3936 3360
Size: 576 5761
MluI A'CGCG_T
Cuts at: 6213 6213
Size: 6337
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2343 5451 5635 1305
Size: 1038 3108 184 2007
Fragments arranged by size:
3108 2007 1038 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2888 10
Size: 54 1425 343 1056 3459
Fragments arranged by size:
3459 1425 1056 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3269 4295 4654 4813 518
Size: 648 2103 1026 359 159 2042
Fragments arranged by size:
2103 2042 1026 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3101 3473 3753 4430 5578 5823 1606
Size: 1495 372 280 677 1148 245 2120
Fragments arranged by size:
2120 1495 1148 677 372 280 245
NcoI C'CATG_G
Cuts at: 513 1065 2112 513
Size: 552 1047 4738
Fragments arranged by size:
4738 1047 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6337
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6337
NotI GC'GGCC_GC
Cuts at: 3784 3784
Size: 6337
NspI r_CATG'y
Cuts at: 1132 1229 3695 5240 1132
Size: 97 2466 1545 2229
Fragments arranged by size:
2466 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6337
PciI A'CATG_T
Cuts at: 5236 5236
Size: 6337
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6337
PmeI GTTT'AAAC
Cuts at: 3641 3641
Size: 6337
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4406 4438 5919 5951 4406
Size: 32 1481 32 4792
Fragments arranged by size:
4792 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2791 2791
Size: 6337
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6337
PsiI TTA'TAA
Cuts at: 3907 3907
Size: 6337
PstI C_TGCA'G
Cuts at: 830 3110 3689 830
Size: 2280 579 3478
Fragments arranged by size:
3478 2280 579
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6337
PvuII CAG'CTG
Cuts at: 3101 3473 3101
Size: 372 5965
SacI G_AGCT'C
Cuts at: 3450 3450
Size: 6337
SalI G'TCGA_C
Cuts at: 1936 3679 1936
Size: 1743 4594
SanDI GG'GwC_CC
Cuts at: 2791 2791
Size: 6337
SbfI CC_TGCA'GG
Cuts at: 3689 3689
Size: 6337
ScaI AGT'ACT
Cuts at: 2698 4585 6297 2698
Size: 1887 1712 2738
Fragments arranged by size:
2738 1887 1712
SexAI A'CCwGG_T
Cuts at: 3147 3147
Size: 6337
SfcI C'TryA_G
Cuts at: 826 1045 3106 3685 4820 5501 5692 826
Size: 219 2061 579 1135 681 191 1471
Fragments arranged by size:
2061 1471 1135 681 579 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6337
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6337
SmaI CCC'GGG
Cuts at: 1872 3664 1872
Size: 1792 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3503 4441 5342 5604 820
Size: 197 926 1560 938 901 262 1553
Fragments arranged by size:
1560 1553 938 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4128 493
Size: 3635 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6337
SphI G_CATG'C
Cuts at: 1132 3695 1132
Size: 2563 3774
SspI AAT'ATT
Cuts at: 5 52 2977 3181 4261 5
Size: 47 2925 204 1080 2081
Fragments arranged by size:
2925 2081 1080 204 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6337
StyI C'CwwG_G
Cuts at: 513 1065 2112 3534 3776 513
Size: 552 1047 1422 242 3074
Fragments arranged by size:
3074 1422 1047 552 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2996 4364 4381 4534 4719 5164 325
Size: 1255 1416 1368 17 153 185 445 1498
Fragments arranged by size:
1498 1416 1368 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2474 2696 2703
Size: 274 80 33 51 1443 497 222 7
Cuts at: 2703 4583 6295 96
Size: 1880 1712 138
Fragments arranged by size:
1880 1712 1443 497 274 222 138 80
51 33 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2269 2292 2301 2324 2868 2900 6297 6329 2269
Size: 23 9 23 544 32 3397 32 2277
Fragments arranged by size:
3397 2277 544 32 32 23 23 9
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6337
XbaI T'CTAG_A
Cuts at: 2362 3673 2362
Size: 1311 5026
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6337
XmaI C'CCGG_G
Cuts at: 1870 3662 1870
Size: 1792 4545
XmnI GAAnn'nnTTC
Cuts at: 4466 4466
Size: 6337
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4145 276
Size: 53 83 186 936 2611 2468
Fragments arranged by size:
2611 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII
SapI SfiI SfoI SrfI SwaI XcmI