Restriction map for
pFN21AB5946
(Circular) (Six-base) MAPSORT of: pFN21AB5946.seq Check: 7702 from: 1 to: 6190
pFN21AB5946 6190 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4000 278
Size: 53 83 186 936 2464 2468
Fragments arranged by size:
2468 2464 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3533 1937
Size: 1596 4594
Acc65I G'GTAC_C
Cuts at: 2151 3511 2151
Size: 1360 4830
AclI AA'CG_TT
Cuts at: 1563 4317 4690 1563
Size: 2754 373 3063
Fragments arranged by size:
3063 2754 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2420 2947 2984 3426 4253 5637 5905 1632
Size: 788 527 37 442 827 1384 268 1917
Fragments arranged by size:
1917 1384 827 788 527 442 268 37
AflII C'TTAA_G
Cuts at: 820 1017 2438 2972 820
Size: 197 1421 534 4038
Fragments arranged by size:
4038 1421 534 197
AflIII A'CryG_T
Cuts at: 1203 2228 5089 6066 1203
Size: 1025 2861 977 1327
Fragments arranged by size:
2861 1327 1025 977
AgeI A'CCGG_T
Cuts at: 5021 5021
Size: 6190
AhdI GACnn_n'nnGTC
Cuts at: 4919 4919
Size: 6190
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2627 1184
Size: 1443 4747
AlwNI CAG_nnn'CTG
Cuts at: 2024 3933 5505 2024
Size: 1909 1572 2709
Fragments arranged by size:
2709 1909 1572
ApaI G_GGCC'C
Cuts at: 2398 2398
Size: 6190
ApaLI G'TGCA_C
Cuts at: 4247 5403 4247
Size: 1156 5034
ApoI r'AATT_y
Cuts at: 1493 3221 3350 3499 3725 3877 5986 1493
Size: 1728 129 149 226 152 2109 1697
Fragments arranged by size:
2109 1728 1697 226 152 149 129
AseI AT'TA_AT
Cuts at: 160 1032 4744 160
Size: 872 3712 1606
Fragments arranged by size:
3712 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
AvaI C'yCGr_G
Cuts at: 1870 1943 2708 3515 1870
Size: 73 765 807 4545
Fragments arranged by size:
4545 807 765 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2215 2248 2215
Size: 33 6157
BamHI G'GATC_C
Cuts at: 3068 3520 3887 3068
Size: 452 367 5371
Fragments arranged by size:
5371 452 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2151 3511 4966 618
Size: 325 217 627 364 1360 1455 1842
Fragments arranged by size:
1842 1455 1360 627 364 325 217
BanII G_rGCy'C
Cuts at: 1391 2166 2398 2756 3021 3509 1391
Size: 775 232 358 265 488 4072
Fragments arranged by size:
4072 775 488 358 265 232
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2001 928
Size: 971 102 5117
Fragments arranged by size:
5117 971 102
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4381 4415 1903
Size: 34 2444 34 3678
Fragments arranged by size:
3678 2444 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4082 5292 4082
Size: 1210 4980
BclI T'GATC_A
Cuts at: 1548 3196 1548
Size: 1648 4542
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4801 136
Size: 107 122 71 631 605 3129 1525
Fragments arranged by size:
3129 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2107 6185 2107
Size: 4078 2112
BlpI GC'TnA_GC
Cuts at: 2882 3607 2882
Size: 725 5465
Bme1580I G_kGCm'C
Cuts at: 1790 2398 3447 4251 5407 1790
Size: 608 1049 804 1156 2573
Fragments arranged by size:
2573 1156 1049 804 608
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6190
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2034 3096 3921
Size: 416 53 83 399 24 617 1062 825
Cuts at: 3921 4879 442
Size: 958 1753
Fragments arranged by size:
1753 1062 958 825 617 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6190
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3152 3152
Size: 6190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2742 3031 3173 4850 1239
Size: 1503 289 142 1677 2579
Fragments arranged by size:
2579 1677 1503 289 142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2799 4315 5180 5478 2799
Size: 1516 865 298 3511
Fragments arranged by size:
3511 1516 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4853 882
Size: 617 3354 2219
Fragments arranged by size:
3354 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3981 493
Size: 711 2777 2702
Fragments arranged by size:
2777 2702 711
BsaBI GATnn'nnATC
Cuts at: 2106 6184 2106
Size: 4078 2112
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3997 4379 5014 275
Size: 53 83 186 936 2464 382 635 1451
Fragments arranged by size:
2464 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4623 5021 5295 5442 5853 4623
Size: 398 274 147 411 4960
Fragments arranged by size:
4960 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2127 2157 2507 2537 2740
Size: 30 165 30 724 30 350 30 203
Cuts at: 2740 2770 1178
Size: 30 4598
Fragments arranged by size:
4598 724 350 203 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 1186
Size: 970 5220
BseYI C'CCAG_C
Cuts at: 5393 5889 5393
Size: 496 5694
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5617
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2344 3640 4401 5429 1540
Size: 275 186 343 1296 761 1028 2301
Fragments arranged by size:
2301 1296 1028 761 343 275 186
BsiHKAI G_wGCw'C
Cuts at: 2756 2888 3021 3234 4251 4336 5407 2756
Size: 132 133 213 1017 85 1071 3539
Fragments arranged by size:
3539 1071 1017 213 133 132 85
BsmI GAATG_Cn'
Cuts at: 2352 3701 3794 2352
Size: 1349 93 4748
Fragments arranged by size:
4748 1349 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2166 2398 2756 2888 3021 3234 3447
Size: 399 376 232 358 132 133 213 213
Cuts at: 3447 3509 4251 4336 5407 1391
Size: 62 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 376 358 232 213
213 133 132 85 62
BspEI T'CCGG_A
Cuts at: 5853 5853
Size: 6190
BspHI T'CATG_A
Cuts at: 3147 4079 5809 3147
Size: 932 1730 3528
Fragments arranged by size:
3528 1730 932
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3545 844
Size: 2701 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4077 1394
Size: 429 2254 3507
Fragments arranged by size:
3507 2254 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4685 4859 65
Size: 1183 3437 174 1396
Fragments arranged by size:
3437 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4834 5021 1700
Size: 110 3024 187 2869
Fragments arranged by size:
3024 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2379 96
Size: 2283 3907
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6190
BssSI C'ACGA_G
Cuts at: 4250 5262 4250
Size: 1012 5178
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6190
BstBI TT'CG_AA
Cuts at: 4994 4994
Size: 6190
BstEII G'GTnAC_C
Cuts at: 1184 2636 1184
Size: 1452 4738
BstXI CCAn_nnnn'nTGG
Cuts at: 2648 3079 2648
Size: 431 5759
BstYI r'GATC_y
Cuts at: 1972 2107 3068 3520 3887 4272 4289 5741 5827
Size: 135 961 452 367 385 17 1452 86
Cuts at: 5827 5839 6185 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 961 452 385 367 346 135
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5638
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5451
Fragments arranged by size:
5451 683 56
BtsI GCAGTG_nn'
Cuts at: 3706 4500 4520 3706
Size: 794 20 5376
Fragments arranged by size:
5376 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6190
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6155
DraI TTT'AAA
Cuts at: 2046 2080 3494 2046
Size: 34 1414 4742
Fragments arranged by size:
4742 1414 34
DraIII CAC_nnn'GTG
Cuts at: 3324 3324
Size: 6190
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5197 809
Size: 507 3881 1802
Fragments arranged by size:
3881 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2650 3637 4526
Size: 54 1425 50 275 18 820 987 889
Cuts at: 4526 6155 8
Size: 1629 43
Fragments arranged by size:
1629 1425 987 889 820 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3637 1537
Size: 275 1825 4090
Fragments arranged by size:
4090 1825 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2109 2930 4120 1353
Size: 756 821 1190 3423
Fragments arranged by size:
3423 1190 821 756
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4777 5151 5297 387
Size: 1064 138 3188 374 146 1280
Fragments arranged by size:
3188 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2754 3019 2754
Size: 265 5925
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2420 2742 2947 2984 3031 3173 3426
Size: 393 788 322 205 37 47 142 253
Cuts at: 3426 4253 4850 5637 5905 1239
Size: 827 597 787 268 1524
Fragments arranged by size:
1524 827 788 787 597 393 322 268
253 205 142 47 37
EcoO109I rG'GnC_Cy
Cuts at: 2394 2395 2394
Size: 1 6189
EcoRI G'AATT_C
Cuts at: 3499 3877 3499
Size: 378 5812
FspI TGC'GCA
Cuts at: 4696 4696
Size: 6190
HaeII r_GCGC'y
Cuts at: 1678 5337 1678
Size: 3659 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2129 2161 2740 2772 4831
Size: 32 135 32 557 32 579 32 2059
Cuts at: 4831 4863 4905 4937 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2059 579 557 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3534 3780 669
Size: 988 281 1596 246 3079
Fragments arranged by size:
3079 1596 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6190
HpaI GTT'AAC
Cuts at: 3780 3780
Size: 6190
KpnI G_GTAC'C
Cuts at: 2155 3515 2155
Size: 1360 4830
MfeI C'AATT_G
Cuts at: 3789 3789
Size: 6190
MluI A'CGCG_T
Cuts at: 6066 6066
Size: 6190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2293 5304 5488 1305
Size: 988 3011 184 2007
Fragments arranged by size:
3011 2007 988 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2652 10
Size: 54 1425 343 820 3548
Fragments arranged by size:
3548 1425 820 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2603 2609 3395 3432 3467 4148 4507
Size: 648 1437 6 786 37 35 681 359
Cuts at: 4507 4666 518
Size: 159 2042
Fragments arranged by size:
2042 1437 786 681 648 359 159 37
35 6
MspA1I CmG'CkG
Cuts at: 1606 3271 3606 4283 5431 5676 1606
Size: 1665 335 677 1148 245 2120
Fragments arranged by size:
2120 1665 1148 677 335 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5638
NdeI CA'TA_TG
Cuts at: 387 2647 387
Size: 2260 3930
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6190
NotI GC'GGCC_GC
Cuts at: 3637 3637
Size: 6190
NspI r_CATG'y
Cuts at: 1132 1229 2232 3479 3548 5093 1132
Size: 97 1003 1247 69 1545 2229
Fragments arranged by size:
2229 1545 1247 1003 97 69
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6190
PciI A'CATG_T
Cuts at: 2228 5089 2228
Size: 2861 3329
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6190
PfoI T'CCnGG_A
Cuts at: 3285 3285
Size: 6190
PmeI GTTT'AAAC
Cuts at: 3494 3494
Size: 6190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4259 4291 5772 5804 4259
Size: 32 1481 32 4645
Fragments arranged by size:
4645 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2577 1613
Size: 964 5226
PsiI TTA'TAA
Cuts at: 3760 3760
Size: 6190
PspOMI G'GGCC_C
Cuts at: 2394 2394
Size: 6190
PstI C_TGCA'G
Cuts at: 830 3542 830
Size: 2712 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6190
PvuII CAG'CTG
Cuts at: 3271 3271
Size: 6190
SacI G_AGCT'C
Cuts at: 2756 3021 2756
Size: 265 5925
SalI G'TCGA_C
Cuts at: 1936 3532 1936
Size: 1596 4594
SapI GCTCTTCn'nnn_
Cuts at: 2109 2109
Size: 6190
SbfI CC_TGCA'GG
Cuts at: 3542 3542
Size: 6190
ScaI AGT'ACT
Cuts at: 4438 6150 4438
Size: 1712 4478
SfcI C'TryA_G
Cuts at: 826 1045 2562 3538 4673 5354 5545 826
Size: 219 1517 976 1135 681 191 1471
Fragments arranged by size:
1517 1471 1135 976 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6190
SmaI CCC'GGG
Cuts at: 1872 3517 1872
Size: 1645 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2438 2814 2972 4294 5195 5457
Size: 197 926 495 376 158 1322 901 262
Cuts at: 5457 820
Size: 1553
Fragments arranged by size:
1553 1322 926 901 495 376 262 197
158
SnaBI TAC'GTA
Cuts at: 493 3981 493
Size: 3488 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6190
SphI G_CATG'C
Cuts at: 1132 3479 3548 1132
Size: 2347 69 3774
Fragments arranged by size:
3774 2347 69
SspI AAT'ATT
Cuts at: 5 52 4114 5
Size: 47 4062 2081
Fragments arranged by size:
4062 2081 47
StuI AGG'CCT
Cuts at: 1517 2532 1517
Size: 1015 5175
StyI C'CwwG_G
Cuts at: 513 1065 3629 513
Size: 552 2564 3074
Fragments arranged by size:
3074 2564 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2358 4217 4234 4387 4572 5017 325
Size: 1255 778 1859 17 153 185 445 1498
Fragments arranged by size:
1859 1498 1255 778 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2379 4436 6148
Size: 274 80 33 51 1443 402 2057 1712
Cuts at: 6148 96
Size: 138
Fragments arranged by size:
2057 1712 1443 402 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2127 2159 2507 2539 6150 6182 2127
Size: 32 348 32 3611 32 2135
Fragments arranged by size:
3611 2135 348 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6190
XbaI T'CTAG_A
Cuts at: 3526 3526
Size: 6190
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6190
XmaI C'CCGG_G
Cuts at: 1870 3515 1870
Size: 1645 4545
XmnI GAAnn'nnTTC
Cuts at: 4319 4319
Size: 6190
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3998 276
Size: 53 83 186 936 2464 2468
Fragments arranged by size:
2468 2464 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI