Restriction map for
pFN21AB5945
(Circular) (Six-base) MAPSORT of: pFN21AB5945.seq Check: 1770 from: 1 to: 6232
pFN21AB5945 6232 bp
With 182 enzymes: SgfI *
March 28, 2012 14:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4042 278
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3575 1937
Size: 1638 4594
Acc65I G'GTAC_C
Cuts at: 3553 3553
Size: 6232
AclI AA'CG_TT
Cuts at: 1563 4359 4732 1563
Size: 2796 373 3063
Fragments arranged by size:
3063 2796 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2672 3278 3548 4295 5679 5947 1632
Size: 1040 606 270 747 1384 268 1917
Fragments arranged by size:
1917 1384 1040 747 606 270 268
AfeI AGC'GCT
Cuts at: 2672 2672
Size: 6232
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6035
AflIII A'CryG_T
Cuts at: 1203 2314 2874 3453 5131 6108 1203
Size: 1111 560 579 1678 977 1327
Fragments arranged by size:
1678 1327 1111 977 579 560
AgeI A'CCGG_T
Cuts at: 5063 5063
Size: 6232
AhdI GACnn_n'nnGTC
Cuts at: 2318 4961 2318
Size: 2643 3589
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3263 1184
Size: 2079 4153
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2424 2456 2424
Size: 32 6200
AlwNI CAG_nnn'CTG
Cuts at: 3975 5547 3975
Size: 1572 4660
ApaI G_GGCC'C
Cuts at: 3238 3238
Size: 6232
ApaLI G'TGCA_C
Cuts at: 4289 5445 4289
Size: 1156 5076
ApoI r'AATT_y
Cuts at: 1493 2822 3541 3767 3919 6028 1493
Size: 1329 719 226 152 2109 1697
Fragments arranged by size:
2109 1697 1329 719 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4786 160
Size: 872 3754 1606
Fragments arranged by size:
3754 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
AvaI C'yCGr_G
Cuts at: 1870 1943 3045 3231 3557 1870
Size: 73 1102 186 326 4545
Fragments arranged by size:
4545 1102 326 186 73
BamHI G'GATC_C
Cuts at: 3562 3929 3562
Size: 367 5865
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2951 3065 3553 5008 618
Size: 325 217 627 1164 114 488 1455 1842
Fragments arranged by size:
1842 1455 1164 627 488 325 217 114
BanII G_rGCy'C
Cuts at: 1391 2764 2898 3238 3523 3551 1391
Size: 1373 134 340 285 28 4072
Fragments arranged by size:
4072 1373 340 285 134 28
BbeI G_GCGC'C
Cuts at: 2955 2955
Size: 6232
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2163 928
Size: 971 264 4997
Fragments arranged by size:
4997 971 264
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4423 4457 1903
Size: 34 2486 34 3678
Fragments arranged by size:
3678 2486 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3039 4124 5334 3039
Size: 1085 1210 3937
Fragments arranged by size:
3937 1210 1085
BclI T'GATC_A
Cuts at: 1548 3327 1548
Size: 1779 4453
BfrBI ATG'CAT
Cuts at: 2549 2549
Size: 6232
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4843 136
Size: 107 122 71 631 605 3171 1525
Fragments arranged by size:
3171 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6227 6227
Size: 6232
BlpI GC'TnA_GC
Cuts at: 3649 3649
Size: 6232
Bme1580I G_kGCm'C
Cuts at: 1790 2061 3070 3238 4293 5449 1790
Size: 271 1009 168 1055 1156 2573
Fragments arranged by size:
2573 1156 1055 1009 271 168
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6232
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3963 4921 442
Size: 416 53 83 399 24 2546 958 1753
Fragments arranged by size:
2546 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6232
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2585 2585
Size: 6232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4892 1239
Size: 3653 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2675 3057 3272 4357 5222 5520 2675
Size: 382 215 1085 865 298 3387
Fragments arranged by size:
3387 1085 865 382 298 215
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4895 882
Size: 617 3396 2219
Fragments arranged by size:
3396 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4023 493
Size: 711 2819 2702
Fragments arranged by size:
2819 2702 711
BsaBI GATnn'nnATC
Cuts at: 3357 6226 3357
Size: 2869 3363
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2952 4039 4421 5056
Size: 53 83 186 936 1419 1087 382 635
Cuts at: 5056 275
Size: 1451
Fragments arranged by size:
1451 1419 1087 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4665 5063 5337 5484 5895 4665
Size: 398 274 147 411 5002
Fragments arranged by size:
5002 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6007
Fragments arranged by size:
6007 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2477 2672 3275 3522 1186
Size: 1291 195 603 247 3896
Fragments arranged by size:
3896 1291 603 247 195
BseYI C'CCAG_C
Cuts at: 2140 5435 5931 2140
Size: 3295 496 2441
Fragments arranged by size:
3295 2441 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2091 2489 1163
Size: 573 355 398 4906
Fragments arranged by size:
4906 573 398 355
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3682 4443 5471 1540
Size: 275 186 1681 761 1028 2301
Fragments arranged by size:
2301 1681 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2664 2696 2764 2898 4293 4378 5449 2664
Size: 32 68 134 1395 85 1071 3447
Fragments arranged by size:
3447 1395 1071 134 85 68 32
BsmI GAATG_Cn'
Cuts at: 3743 3836 3743
Size: 93 6139
BsmBI CGTCTCn'nnnn_
Cuts at: 3498 3498
Size: 6232
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2664 2696 2764 2898 3070 3238
Size: 399 271 603 32 68 134 172 168
Cuts at: 3238 3523 3551 4293 4378 5449 1391
Size: 285 28 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 603 399 285 271 172
168 134 85 68 32 28
BspEI T'CCGG_A
Cuts at: 5895 5895
Size: 6232
BspHI T'CATG_A
Cuts at: 2107 4121 5851 2107
Size: 2014 1730 2488
Fragments arranged by size:
2488 2014 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3587 844
Size: 2743 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3273 4119 1394
Size: 429 1450 846 3507
Fragments arranged by size:
3507 1450 846 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2794 3247 4727 4901 65
Size: 1183 1546 453 1480 174 1396
Fragments arranged by size:
1546 1480 1396 1183 453 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4876 5063 1700
Size: 110 3066 187 2869
Fragments arranged by size:
3066 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6232
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6232
BssSI C'ACGA_G
Cuts at: 4292 5304 4292
Size: 1012 5220
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2056 2069 794
Size: 1262 13 4957
Fragments arranged by size:
4957 1262 13
BstBI TT'CG_AA
Cuts at: 5036 5036
Size: 6232
BstEII G'GTnAC_C
Cuts at: 1184 2480 1184
Size: 1296 4936
BstYI r'GATC_y
Cuts at: 1972 3562 3929 4314 4331 5783 5869 5881 6227
Size: 1590 367 385 17 1452 86 12 346
Cuts at: 6227 1972
Size: 1977
Fragments arranged by size:
1977 1590 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2232 3162 3424 513
Size: 552 939 228 930 262 3321
Fragments arranged by size:
3321 939 930 552 262 228
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2379 487
Size: 683 56 1153 4340
Fragments arranged by size:
4340 1153 683 56
BtsI GCAGTG_nn'
Cuts at: 2709 2842 3370 3748 4542 4562 2709
Size: 133 528 378 794 20 4379
Fragments arranged by size:
4379 794 528 378 133 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2772 2807 3130 3165 529
Size: 35 2208 35 323 35 3596
Fragments arranged by size:
3596 2208 323 35 35 35
DraI TTT'AAA
Cuts at: 2220 3536 2220
Size: 1316 4916
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5239 809
Size: 507 3923 1802
Fragments arranged by size:
3923 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3151 3679 4568
Size: 54 1425 50 275 18 1321 528 889
Cuts at: 4568 6197 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1321 889 528 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3679 1537
Size: 275 1867 4090
Fragments arranged by size:
4090 1867 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2241 2554 3104 3389 4162 1353
Size: 888 313 550 285 773 3423
Fragments arranged by size:
3423 888 773 550 313 285
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2480 4819 5193 5339 387
Size: 1064 138 891 2339 374 146 1280
Fragments arranged by size:
2339 1280 1064 891 374 146 138
EcoICRI GAG'CTC
Cuts at: 2762 2896 2762
Size: 134 6098
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2672 3278 3548 4295 4892 5679 5947
Size: 393 1040 606 270 747 597 787 268
Cuts at: 5947 1239
Size: 1524
Fragments arranged by size:
1524 1040 787 747 606 597 393 270
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3527 3527
Size: 6232
EcoRI G'AATT_C
Cuts at: 3541 3919 3541
Size: 378 5854
EcoRV GAT'ATC
Cuts at: 3464 3464
Size: 6232
FspI TGC'GCA
Cuts at: 4738 4738
Size: 6232
HaeII r_GCGC'y
Cuts at: 1678 2674 2842 2955 5379 1678
Size: 996 168 113 2424 2531
Fragments arranged by size:
2531 2424 996 168 113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2304 2336 3303 3335 3476
Size: 32 135 32 732 32 967 32 141
Cuts at: 3476 3508 4873 4905 4947 4979 1373
Size: 32 1365 32 42 32 2626
Fragments arranged by size:
2626 1365 967 732 141 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2857 3576 3822 669
Size: 988 281 919 719 246 3079
Fragments arranged by size:
3079 988 919 719 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6232
HpaI GTT'AAC
Cuts at: 3822 3822
Size: 6232
KasI G'GCGC_C
Cuts at: 2951 2951
Size: 6232
KpnI G_GTAC'C
Cuts at: 3557 3557
Size: 6232
MfeI C'AATT_G
Cuts at: 3831 3831
Size: 6232
MluI A'CGCG_T
Cuts at: 6108 6108
Size: 6232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2286 5346 5530 1305
Size: 981 3060 184 2007
Fragments arranged by size:
3060 2007 981 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3153 10
Size: 54 1425 343 1321 3089
Fragments arranged by size:
3089 1425 1321 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2427 2443 4190 4549 4708 518
Size: 648 1261 16 1747 359 159 2042
Fragments arranged by size:
2042 1747 1261 648 359 159 16
MspA1I CmG'CkG
Cuts at: 1606 2075 3648 4325 5473 5718 1606
Size: 469 1573 677 1148 245 2120
Fragments arranged by size:
2120 1573 1148 677 469 245
NarI GG'CG_CC
Cuts at: 2952 2952
Size: 6232
NcoI C'CATG_G
Cuts at: 513 1065 2004 2232 513
Size: 552 939 228 4513
Fragments arranged by size:
4513 939 552 228
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6232
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6232
NotI GC'GGCC_GC
Cuts at: 3679 3679
Size: 6232
NsiI A_TGCA'T
Cuts at: 2551 2551
Size: 6232
NspI r_CATG'y
Cuts at: 1132 1229 2318 2553 2878 3028 3457 3590 5135
Size: 97 1089 235 325 150 429 133 1545
Cuts at: 5135 1132
Size: 2229
Fragments arranged by size:
2229 1545 1089 429 325 235 150 133
97
PasI CC'CwG_GG
Cuts at: 1348 2702 1348
Size: 1354 4878
PciI A'CATG_T
Cuts at: 2314 2874 3453 5131 2314
Size: 560 579 1678 3415
Fragments arranged by size:
3415 1678 579 560
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2348 1108
Size: 1240 4992
PfoI T'CCnGG_A
Cuts at: 3387 3387
Size: 6232
PmeI GTTT'AAAC
Cuts at: 3536 3536
Size: 6232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4301 4333 5814 5846 4301
Size: 32 1481 32 4687
Fragments arranged by size:
4687 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6232
PsiI TTA'TAA
Cuts at: 3802 3802
Size: 6232
PspOMI G'GGCC_C
Cuts at: 3234 3234
Size: 6232
PstI C_TGCA'G
Cuts at: 830 3584 830
Size: 2754 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6232
SacI G_AGCT'C
Cuts at: 2764 2898 2764
Size: 134 6098
SalI G'TCGA_C
Cuts at: 1936 3574 1936
Size: 1638 4594
SapI GCTCTTCn'nnn_
Cuts at: 2241 3104 2241
Size: 863 5369
SbfI CC_TGCA'GG
Cuts at: 3584 3584
Size: 6232
ScaI AGT'ACT
Cuts at: 4480 6192 4480
Size: 1712 4520
SfcI C'TryA_G
Cuts at: 826 1045 2089 3580 4715 5396 5587 826
Size: 219 1044 1491 1135 681 191 1471
Fragments arranged by size:
1491 1471 1135 1044 681 219 191
SfoI GGC'GCC
Cuts at: 2953 2953
Size: 6232
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6232
SmaI CCC'GGG
Cuts at: 1872 3047 3559 1872
Size: 1175 512 4545
Fragments arranged by size:
4545 1175 512
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2654 3072 3287 4336 5237 5499
Size: 197 926 711 418 215 1049 901 262
Cuts at: 5499 820
Size: 1553
Fragments arranged by size:
1553 1049 926 901 711 418 262 215
197
SnaBI TAC'GTA
Cuts at: 493 4023 493
Size: 3530 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6232
SphI G_CATG'C
Cuts at: 1132 2553 3590 1132
Size: 1421 1037 3774
Fragments arranged by size:
3774 1421 1037
SspI AAT'ATT
Cuts at: 5 52 4156 5
Size: 47 4104 2081
Fragments arranged by size:
4104 2081 47
StuI AGG'CCT
Cuts at: 1517 3132 1517
Size: 1615 4617
StyI C'CwwG_G
Cuts at: 513 1065 2004 2232 2970 3671 513
Size: 552 939 228 738 701 3074
Fragments arranged by size:
3074 939 738 701 552 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4259 4276 4429 4614 5059 325
Size: 1255 2679 17 153 185 445 1498
Fragments arranged by size:
2679 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4478 6190 96
Size: 274 80 33 51 1443 2501 1712 138
Fragments arranged by size:
2501 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6192 6224 6192
Size: 32 6200
Tth111I GACn'n_nGTC
Cuts at: 1316 3317 1316
Size: 2001 4231
XbaI T'CTAG_A
Cuts at: 3568 3568
Size: 6232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3001 3001
Size: 6232
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6232
XmaI C'CCGG_G
Cuts at: 1870 3045 3557 1870
Size: 1175 512 4545
Fragments arranged by size:
4545 1175 512
XmnI GAAnn'nnTTC
Cuts at: 4361 4361
Size: 6232
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4040 276
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI Bpu10I BsiWI BstXI
BstZ17I Bsu36I DraIII EcoO109I FalI FseI FspAI NaeI
NgoMIV NruI PacI PmlI PpuMI PspXI PsrI PvuII
RsrII SacII SanDI SexAI SfiI SrfI SwaI