Restriction map for
pFN21AB5943
(Circular) (Six-base) MAPSORT of: pFN21AB5943.seq Check: 9506 from: 1 to: 6229
pFN21AB5943 6229 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4039 278
Size: 53 83 186 936 2503 2468
Fragments arranged by size:
2503 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3572 1937
Size: 1635 4594
Acc65I G'GTAC_C
Cuts at: 3550 3550
Size: 6229
AclI AA'CG_TT
Cuts at: 1563 4356 4729 1563
Size: 2793 373 3063
Fragments arranged by size:
3063 2793 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2730 2804 4292 5676 5944 1632
Size: 1098 74 1488 1384 268 1917
Fragments arranged by size:
1917 1488 1384 1098 268 74
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6032
AflIII A'CryG_T
Cuts at: 1203 5128 6105 1203
Size: 3925 977 1327
Fragments arranged by size:
3925 1327 977
AgeI A'CCGG_T
Cuts at: 2350 5060 2350
Size: 2710 3519
AhdI GACnn_n'nnGTC
Cuts at: 4958 4958
Size: 6229
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2079 1184
Size: 895 5334
AlwNI CAG_nnn'CTG
Cuts at: 2591 3972 5544 2591
Size: 1381 1572 3276
Fragments arranged by size:
3276 1572 1381
ApaLI G'TGCA_C
Cuts at: 4286 5442 4286
Size: 1156 5073
ApoI r'AATT_y
Cuts at: 1493 2096 2643 2855 3477 3538 3764 3916 6025
Size: 603 547 212 622 61 226 152 2109
Cuts at: 6025 1493
Size: 1697
Fragments arranged by size:
2109 1697 622 603 547 226 212 152
61
AseI AT'TA_AT
Cuts at: 160 1032 4783 160
Size: 872 3751 1606
Fragments arranged by size:
3751 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
AvaI C'yCGr_G
Cuts at: 1870 1943 2242 3554 1870
Size: 73 299 1312 4545
Fragments arranged by size:
4545 1312 299 73
BamHI G'GATC_C
Cuts at: 2008 3559 3926 2008
Size: 1551 367 4311
Fragments arranged by size:
4311 1551 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2063 3550 5005 618
Size: 325 217 627 276 1487 1455 1842
Fragments arranged by size:
1842 1487 1455 627 325 276 217
BanII G_rGCy'C
Cuts at: 1391 2036 2205 2307 3548 1391
Size: 645 169 102 1241 4072
Fragments arranged by size:
4072 1241 645 169 102
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2107 2432 2565 2792 2901 928
Size: 971 208 325 133 227 109 4256
Fragments arranged by size:
4256 971 325 227 208 133 109
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3311 3345 4420 4454 1903
Size: 34 1374 34 1075 34 3678
Fragments arranged by size:
3678 1374 1075 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4121 5331 4121
Size: 1210 5019
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6229
BfrBI ATG'CAT
Cuts at: 2334 2334
Size: 6229
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2072 4840 136
Size: 107 122 71 631 605 400 2768 1525
Fragments arranged by size:
2768 1525 631 605 400 122 107 71
BglII A'GATC_T
Cuts at: 2451 6224 2451
Size: 3773 2456
BlpI GC'TnA_GC
Cuts at: 3646 3646
Size: 6229
Bme1580I G_kGCm'C
Cuts at: 1790 3317 4290 5446 1790
Size: 1527 973 1156 2573
Fragments arranged by size:
2573 1527 1156 973
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6229
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2452 3960 4918
Size: 416 53 83 399 24 1035 1508 958
Cuts at: 4918 442
Size: 1753
Fragments arranged by size:
1753 1508 1035 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6229
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2174 2257 2174
Size: 83 6146
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2279 4889 1239
Size: 1040 2610 2579
Fragments arranged by size:
2610 2579 1040
Bpu10I CC'TnA_GC
Cuts at: 3164 3164
Size: 6229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2050 2070 4354 5219 5517 2050
Size: 20 2284 865 298 2762
Fragments arranged by size:
2762 2284 865 298 20
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2041 3366 4892 882
Size: 617 542 1325 1526 2219
Fragments arranged by size:
2219 1526 1325 617 542
BsaAI yAC'GTr
Cuts at: 493 1204 4020 493
Size: 711 2816 2702
Fragments arranged by size:
2816 2702 711
BsaBI GATnn'nnATC
Cuts at: 2743 6223 2743
Size: 3480 2749
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4036 4418 5053 275
Size: 53 83 186 936 2503 382 635 1451
Fragments arranged by size:
2503 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2350 2974 3006 4662 5060 5334 5481 5892 2350
Size: 624 32 1656 398 274 147 411 2687
Fragments arranged by size:
2687 1656 624 411 398 274 147 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2289 2319 1178
Size: 30 165 30 886 30 5088
Fragments arranged by size:
5088 886 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2068 2073 2297 2696 1186
Size: 882 5 224 399 4719
Fragments arranged by size:
4719 882 399 224 5
BseYI C'CCAG_C
Cuts at: 5432 5928 5432
Size: 496 5733
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5656
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3679 4440 5468 1540
Size: 275 186 1678 761 1028 2301
Fragments arranged by size:
2301 1678 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2205 2307 4290 4375 5446 2205
Size: 102 1983 85 1071 2988
Fragments arranged by size:
2988 1983 1071 102 85
BsmI GAATG_Cn'
Cuts at: 2336 2871 3740 3833 2336
Size: 535 869 93 4732
Fragments arranged by size:
4732 869 535 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2205 2307 3317 3548 4290 4375
Size: 399 246 169 102 1010 231 742 85
Cuts at: 4375 5446 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1010 742 399 246 231 169
102 85
BspEI T'CCGG_A
Cuts at: 2974 3006 5892 2974
Size: 32 2886 3311
Fragments arranged by size:
3311 2886 32
BspHI T'CATG_A
Cuts at: 4118 5848 4118
Size: 1730 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3584 844
Size: 2740 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3400 4116 1394
Size: 429 1577 716 3507
Fragments arranged by size:
3507 1577 716 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4724 4898 65
Size: 1183 3476 174 1396
Fragments arranged by size:
3476 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2350 4873 5060 1700
Size: 110 540 2523 187 2869
Fragments arranged by size:
2869 2523 540 187 110
BsrGI T'GTAC_A
Cuts at: 96 2443 96
Size: 2347 3882
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6229
BssSI C'ACGA_G
Cuts at: 2016 4289 5301 2016
Size: 2273 1012 2944
Fragments arranged by size:
2944 2273 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6229
BstBI TT'CG_AA
Cuts at: 5033 5033
Size: 6229
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6229
BstXI CCAn_nnnn'nTGG
Cuts at: 3298 3298
Size: 6229
BstYI r'GATC_y
Cuts at: 1972 2008 2451 3559 3926 4311 4328 5780 5866
Size: 36 443 1108 367 385 17 1452 86
Cuts at: 5866 5878 6224 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1108 443 385 367 346 86
36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3512 513
Size: 552 939 1508 3230
Fragments arranged by size:
3230 1508 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5490
Fragments arranged by size:
5490 683 56
BtsI GCAGTG_nn'
Cuts at: 3745 4539 4559 3745
Size: 794 20 5415
Fragments arranged by size:
5415 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6194
DraI TTT'AAA
Cuts at: 2487 3533 2487
Size: 1046 5183
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5236 809
Size: 507 3920 1802
Fragments arranged by size:
3920 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3676 4565 6194
Size: 54 1425 50 275 18 1846 889 1629
Cuts at: 6194 8
Size: 43
Fragments arranged by size:
1846 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3676 1537
Size: 275 1864 4090
Fragments arranged by size:
4090 1864 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2039 2383 3069 4159 1353
Size: 686 344 686 1090 3423
Fragments arranged by size:
3423 1090 686 686 344
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4816 5190 5336 387
Size: 1064 138 3227 374 146 1280
Fragments arranged by size:
3227 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2203 2305 2203
Size: 102 6127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2279 2730 2804 4292 4889 5676 5944
Size: 393 647 451 74 1488 597 787 268
Cuts at: 5944 1239
Size: 1524
Fragments arranged by size:
1524 1488 787 647 597 451 393 268
74
EcoO109I rG'GnC_Cy
Cuts at: 2417 2417
Size: 6229
EcoRI G'AATT_C
Cuts at: 2643 3538 3916 2643
Size: 895 378 4956
Fragments arranged by size:
4956 895 378
EcoRV GAT'ATC
Cuts at: 2741 2741
Size: 6229
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2499 3098 2499
Size: 599 5630
FspI TGC'GCA
Cuts at: 3486 4735 3486
Size: 1249 4980
HaeII r_GCGC'y
Cuts at: 1678 3385 5376 1678
Size: 1707 1991 2531
Fragments arranged by size:
2531 1991 1707
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4870 4902 4944 4976 1373
Size: 32 135 32 3298 32 42 32 2626
Fragments arranged by size:
3298 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3150 3573 3819 669
Size: 988 281 1212 423 246 3079
Fragments arranged by size:
3079 1212 988 423 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6229
HpaI GTT'AAC
Cuts at: 3819 3819
Size: 6229
KpnI G_GTAC'C
Cuts at: 3554 3554
Size: 6229
MfeI C'AATT_G
Cuts at: 2436 3828 2436
Size: 1392 4837
MluI A'CGCG_T
Cuts at: 6105 6105
Size: 6229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2820 5343 5527 1305
Size: 1515 2523 184 2007
Fragments arranged by size:
2523 2007 1515 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4407
Fragments arranged by size:
4407 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2624 3296 4187 4546 4705 518
Size: 648 1458 672 891 359 159 2042
Fragments arranged by size:
2042 1458 891 672 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2907 3264 3645 4322 5470 5715 1606
Size: 1301 357 381 677 1148 245 2120
Fragments arranged by size:
2120 1301 1148 677 381 357 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4738
Fragments arranged by size:
4738 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6229
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6229
NotI GC'GGCC_GC
Cuts at: 3676 3676
Size: 6229
NsiI A_TGCA'T
Cuts at: 2336 2336
Size: 6229
NspI r_CATG'y
Cuts at: 1132 1229 3587 5132 1132
Size: 97 2358 1545 2229
Fragments arranged by size:
2358 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6229
PciI A'CATG_T
Cuts at: 5128 5128
Size: 6229
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6229
PmeI GTTT'AAAC
Cuts at: 3533 3533
Size: 6229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2786 2818 4298 4330 5811 5843 2786
Size: 32 1480 32 1481 32 3172
Fragments arranged by size:
3172 1481 1480 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6229
PsiI TTA'TAA
Cuts at: 3799 3799
Size: 6229
PstI C_TGCA'G
Cuts at: 830 3581 830
Size: 2751 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6229
PvuII CAG'CTG
Cuts at: 2907 3264 2907
Size: 357 5872
SacI G_AGCT'C
Cuts at: 2205 2307 2205
Size: 102 6127
SalI G'TCGA_C
Cuts at: 1936 3571 1936
Size: 1635 4594
SapI GCTCTTCn'nnn_
Cuts at: 3069 3069
Size: 6229
SbfI CC_TGCA'GG
Cuts at: 3581 3581
Size: 6229
ScaI AGT'ACT
Cuts at: 4477 6189 4477
Size: 1712 4517
SfcI C'TryA_G
Cuts at: 826 1045 3577 4712 5393 5584 826
Size: 219 2532 1135 681 191 1471
Fragments arranged by size:
2532 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6229
SmaI CCC'GGG
Cuts at: 1872 3556 1872
Size: 1684 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2029 2085 4333 5234 5496 820
Size: 197 926 86 56 2248 901 262 1553
Fragments arranged by size:
2248 1553 926 901 262 197 86 56
SnaBI TAC'GTA
Cuts at: 493 4020 493
Size: 3527 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6229
SphI G_CATG'C
Cuts at: 1132 3587 1132
Size: 2455 3774
SspI AAT'ATT
Cuts at: 5 52 4153 5
Size: 47 4101 2081
Fragments arranged by size:
4101 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6229
StyI C'CwwG_G
Cuts at: 513 1065 2004 2661 3668 513
Size: 552 939 657 1007 3074
Fragments arranged by size:
3074 1007 939 657 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2234 4256 4273 4426 4611 5056 325
Size: 1255 654 2022 17 153 185 445 1498
Fragments arranged by size:
2022 1498 1255 654 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2281 2443 4475
Size: 274 80 33 51 1443 304 162 2032
Cuts at: 4475 6187 96
Size: 1712 138
Fragments arranged by size:
2032 1712 1443 304 274 162 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6189 6221 6189
Size: 32 6197
Tth111I GACn'n_nGTC
Cuts at: 1316 2900 1316
Size: 1584 4645
XbaI T'CTAG_A
Cuts at: 3565 3565
Size: 6229
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6229
XmaI C'CCGG_G
Cuts at: 1870 3554 1870
Size: 1684 4545
XmnI GAAnn'nnTTC
Cuts at: 2183 2643 2801 4358 2183
Size: 460 158 1557 4054
Fragments arranged by size:
4054 1557 460 158
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4037 276
Size: 53 83 186 936 2503 2468
Fragments arranged by size:
2503 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI