Restriction map for
pFN21AB5940
(Circular) (Six-base) MAPSORT of: pFN21AB5940.seq Check: 5238 from: 1 to: 6325
pFN21AB5940 6325 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2475 2475
Size: 6325
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4135 278
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2340 3668 1937
Size: 403 1328 4594
Fragments arranged by size:
4594 1328 403
Acc65I G'GTAC_C
Cuts at: 3646 3646
Size: 6325
AclI AA'CG_TT
Cuts at: 1563 4452 4825 1563
Size: 2889 373 3063
Fragments arranged by size:
3063 2889 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2441 3204 4388 5772 6040 1632
Size: 809 763 1184 1384 268 1917
Fragments arranged by size:
1917 1384 1184 809 763 268
AfeI AGC'GCT
Cuts at: 2151 2151
Size: 6325
AflII C'TTAA_G
Cuts at: 820 1017 3023 820
Size: 197 2006 4122
Fragments arranged by size:
4122 2006 197
AflIII A'CryG_T
Cuts at: 1203 5224 6201 1203
Size: 4021 977 1327
Fragments arranged by size:
4021 1327 977
AgeI A'CCGG_T
Cuts at: 3306 5156 3306
Size: 1850 4475
AhdI GACnn_n'nnGTC
Cuts at: 5054 5054
Size: 6325
AleI CACnn'nnGTG
Cuts at: 1166 2478 1166
Size: 1312 5013
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6325
AlwNI CAG_nnn'CTG
Cuts at: 4068 5640 4068
Size: 1572 4753
ApaI G_GGCC'C
Cuts at: 2952 2952
Size: 6325
ApaLI G'TGCA_C
Cuts at: 4382 5538 4382
Size: 1156 5169
ApoI r'AATT_y
Cuts at: 1493 2311 2712 2763 2849 3124 3333 3634 3860
Size: 818 401 51 86 275 209 301 226
Cuts at: 3860 4012 6121 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 818 401 301 275 226 209
152 86 51
AseI AT'TA_AT
Cuts at: 160 1032 4879 160
Size: 872 3847 1606
Fragments arranged by size:
3847 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 2394 2952 3650 1870
Size: 73 112 339 558 698 4545
Fragments arranged by size:
4545 698 558 339 112 73
BamHI G'GATC_C
Cuts at: 3655 4022 3655
Size: 367 5958
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 3646 5101 618
Size: 325 217 627 335 1524 1455 1842
Fragments arranged by size:
1842 1524 1455 627 335 325 217
BanII G_rGCy'C
Cuts at: 1391 2952 3284 3644 1391
Size: 1561 332 360 4072
Fragments arranged by size:
4072 1561 360 332
BbeI G_GCGC'C
Cuts at: 2126 2126
Size: 6325
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2505 928
Size: 971 606 4748
Fragments arranged by size:
4748 971 606
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3001 3035 4516 4550 1903
Size: 34 1064 34 1481 34 3678
Fragments arranged by size:
3678 1481 1064 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4217 5427 4217
Size: 1210 5115
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6325
BfrBI ATG'CAT
Cuts at: 3058 3058
Size: 6325
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4936 136
Size: 107 122 71 631 605 3264 1525
Fragments arranged by size:
3264 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2209 2783 3538 6320 2209
Size: 574 755 2782 2214
Fragments arranged by size:
2782 2214 755 574
BlpI GC'TnA_GC
Cuts at: 3351 3742 3351
Size: 391 5934
Bme1580I G_kGCm'C
Cuts at: 1790 2952 3251 4386 5542 1790
Size: 1162 299 1135 1156 2573
Fragments arranged by size:
2573 1162 1156 1135 299
BmgBI CAC'GTC
Cuts at: 1318 2997 1318
Size: 1679 4646
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2373 2542 4056
Size: 416 53 83 399 24 956 169 1514
Cuts at: 4056 5014 442
Size: 958 1753
Fragments arranged by size:
1753 1514 958 956 416 399 169 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4985 1239
Size: 3746 2579
Bpu10I CC'TnA_GC
Cuts at: 2253 2253
Size: 6325
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4450 5315 5613 4450
Size: 865 298 5162
Fragments arranged by size:
5162 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2949 4988 882
Size: 617 1450 2039 2219
Fragments arranged by size:
2219 2039 1450 617
BsaAI yAC'GTr
Cuts at: 493 1204 4116 493
Size: 711 2912 2702
Fragments arranged by size:
2912 2702 711
BsaBI GATnn'nnATC
Cuts at: 6319 6319
Size: 6325
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2109 2123 4132 4514
Size: 53 83 186 936 576 14 2009 382
Cuts at: 4514 5149 275
Size: 635 1451
Fragments arranged by size:
2009 1451 936 635 576 382 186 83
53 14
BsaWI w'CCGG_w
Cuts at: 2164 2221 3306 4758 5156 5430 5577 5988 2164
Size: 57 1085 1452 398 274 147 411 2501
Fragments arranged by size:
2501 1452 1085 411 398 274 147 57
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6100
Fragments arranged by size:
6100 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6325
BseYI C'CCAG_C
Cuts at: 3000 3312 5528 6024 3000
Size: 312 2216 496 3301
Fragments arranged by size:
3301 2216 496 312
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2123 2994 1163
Size: 573 387 871 4494
Fragments arranged by size:
4494 871 573 387
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3775 4536 5564 1540
Size: 275 186 1774 761 1028 2301
Fragments arranged by size:
2301 1774 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2466 2905 3284 4386 4471 5542 2466
Size: 439 379 1102 85 1071 3249
Fragments arranged by size:
3249 1102 1071 439 379 85
BsmI GAATG_Cn'
Cuts at: 3836 3929 3836
Size: 93 6232
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2466 2905 2952 3251 3284 3644 4386
Size: 399 676 439 47 299 33 360 742
Cuts at: 4386 4471 5542 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 676 439 399 360 299
85 47 33
BspEI T'CCGG_A
Cuts at: 2164 2221 5988 2164
Size: 57 3767 2501
Fragments arranged by size:
3767 2501 57
BspHI T'CATG_A
Cuts at: 4214 5944 4214
Size: 1730 4595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2475 3680 844
Size: 1631 1205 3489
Fragments arranged by size:
3489 1631 1205
BsrBI CCG'CTC
Cuts at: 1394 1823 4212 1394
Size: 429 2389 3507
Fragments arranged by size:
3507 2389 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3144 4820 4994 65
Size: 1183 1896 1676 174 1396
Fragments arranged by size:
1896 1676 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2125 3306 4969 5156 1700
Size: 110 315 1181 1663 187 2869
Fragments arranged by size:
2869 1663 1181 315 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6325
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6325
BssSI C'ACGA_G
Cuts at: 4385 5397 4385
Size: 1012 5313
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6325
BstBI TT'CG_AA
Cuts at: 5129 5129
Size: 6325
BstEII G'GTnAC_C
Cuts at: 1184 3004 1184
Size: 1820 4505
BstXI CCAn_nnnn'nTGG
Cuts at: 2084 3295 2084
Size: 1211 5114
BstYI r'GATC_y
Cuts at: 1972 2209 2783 3538 3655 4022 4407 4424 5876
Size: 237 574 755 117 367 385 17 1452
Cuts at: 5876 5962 5974 6320 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 755 574 385 367 346 237
117 86 17 12
BstZ17I GTA'TAC
Cuts at: 2341 2341
Size: 6325
BtgI C'CryG_G
Cuts at: 513 1065 2176 2825 3317 513
Size: 552 1111 649 492 3521
Fragments arranged by size:
3521 1111 649 552 492
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5586
Fragments arranged by size:
5586 683 56
BtsI GCAGTG_nn'
Cuts at: 3264 3841 4635 4655 3264
Size: 577 794 20 4934
Fragments arranged by size:
4934 794 577 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6325
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6290
DraI TTT'AAA
Cuts at: 2596 3629 2596
Size: 1033 5292
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5332 809
Size: 507 4016 1802
Fragments arranged by size:
4016 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 3520 3772
Size: 54 1425 50 275 18 240 1450 252
Cuts at: 3772 4661 6290 8
Size: 889 1629 43
Fragments arranged by size:
1629 1450 1425 889 275 252 240 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3772 1537
Size: 275 1960 4090
Fragments arranged by size:
4090 1960 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2976 3277 4255 1353
Size: 1623 301 978 3423
Fragments arranged by size:
3423 1623 978 301
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4912 5286 5432 387
Size: 1064 138 3323 374 146 1280
Fragments arranged by size:
3323 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3282 3282
Size: 6325
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2441 3204 4388 4985 5772 6040 1239
Size: 393 809 763 1184 597 787 268 1524
Fragments arranged by size:
1524 1184 809 787 763 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2475 2475
Size: 6325
EcoO109I rG'GnC_Cy
Cuts at: 2172 2948 2949 2172
Size: 776 1 5548
Fragments arranged by size:
5548 776 1
EcoRI G'AATT_C
Cuts at: 3124 3634 4012 3124
Size: 510 378 5437
Fragments arranged by size:
5437 510 378
FspI TGC'GCA
Cuts at: 4831 4831
Size: 6325
HaeII r_GCGC'y
Cuts at: 1678 2037 2091 2126 2153 5472 1678
Size: 359 54 35 27 3319 2531
Fragments arranged by size:
3319 2531 359 54 35 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4966 4998 5040 5072 1373
Size: 32 135 32 3394 32 42 32 2626
Fragments arranged by size:
3394 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3669 3915 669
Size: 988 281 1731 246 3079
Fragments arranged by size:
3079 1731 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6325
HpaI GTT'AAC
Cuts at: 3915 3915
Size: 6325
KasI G'GCGC_C
Cuts at: 2122 2122
Size: 6325
KpnI G_GTAC'C
Cuts at: 3650 3650
Size: 6325
MfeI C'AATT_G
Cuts at: 3924 3924
Size: 6325
MluI A'CGCG_T
Cuts at: 6201 6201
Size: 6325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2553 5439 5623 1305
Size: 1248 2886 184 2007
Fragments arranged by size:
2886 2007 1248 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3522 10
Size: 54 1425 343 1690 2813
Fragments arranged by size:
2813 1690 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2478 3293 3606 4283 4642 4801 518
Size: 648 1312 815 313 677 359 159 2042
Fragments arranged by size:
2042 1312 815 677 648 359 313 159
MspA1I CmG'CkG
Cuts at: 1606 2068 2178 2410 2448 2827 3040 3550 3741
Size: 462 110 232 38 379 213 510 191
Cuts at: 3741 4418 5566 5811 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 510 462 379 245 232
213 191 110 38
NaeI GCC'GGC
Cuts at: 2127 2127
Size: 6325
NarI GG'CG_CC
Cuts at: 2123 2123
Size: 6325
NcoI C'CATG_G
Cuts at: 513 1065 3317 513
Size: 552 2252 3521
Fragments arranged by size:
3521 2252 552
NdeI CA'TA_TG
Cuts at: 387 2625 387
Size: 2238 4087
NgoMIV G'CCGG_C
Cuts at: 2125 2125
Size: 6325
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6325
NotI GC'GGCC_GC
Cuts at: 3772 3772
Size: 6325
NsiI A_TGCA'T
Cuts at: 3060 3060
Size: 6325
NspI r_CATG'y
Cuts at: 1132 1229 2732 3037 3683 5228 1132
Size: 97 1503 305 646 1545 2229
Fragments arranged by size:
2229 1545 1503 646 305 97
PasI CC'CwG_GG
Cuts at: 1348 2095 1348
Size: 747 5578
PciI A'CATG_T
Cuts at: 5224 5224
Size: 6325
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2119 2509 3511 1108
Size: 1011 390 1002 3922
Fragments arranged by size:
3922 1011 1002 390
PmeI GTTT'AAAC
Cuts at: 3629 3629
Size: 6325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4394 4426 5907 5939 4394
Size: 32 1481 32 4780
Fragments arranged by size:
4780 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2172 2172
Size: 6325
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6325
PsiI TTA'TAA
Cuts at: 3895 3895
Size: 6325
PspOMI G'GGCC_C
Cuts at: 2948 2948
Size: 6325
PstI C_TGCA'G
Cuts at: 830 2191 3086 3677 830
Size: 1361 895 591 3478
Fragments arranged by size:
3478 1361 895 591
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6325
PvuII CAG'CTG
Cuts at: 2410 2448 3040 3550 2410
Size: 38 592 510 5185
Fragments arranged by size:
5185 592 510 38
SacI G_AGCT'C
Cuts at: 3284 3284
Size: 6325
SacII CC_GC'GG
Cuts at: 2179 2828 2179
Size: 649 5676
SalI G'TCGA_C
Cuts at: 1936 3667 1936
Size: 1731 4594
SanDI GG'GwC_CC
Cuts at: 2172 2172
Size: 6325
SapI GCTCTTCn'nnn_
Cuts at: 2976 2976
Size: 6325
SbfI CC_TGCA'GG
Cuts at: 3677 3677
Size: 6325
ScaI AGT'ACT
Cuts at: 4573 6285 4573
Size: 1712 4613
SfcI C'TryA_G
Cuts at: 826 1045 2187 3082 3673 4808 5489 5680 826
Size: 219 1142 895 591 1135 681 191 1471
Fragments arranged by size:
1471 1142 1135 895 681 591 219 191
SfoI GGC'GCC
Cuts at: 2124 2124
Size: 6325
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6325
SmaI CCC'GGG
Cuts at: 1872 2057 3652 1872
Size: 185 1595 4545
Fragments arranged by size:
4545 1595 185
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2394 3023 4429 5330 5592 820
Size: 197 926 451 629 1406 901 262 1553
Fragments arranged by size:
1553 1406 926 901 629 451 262 197
SnaBI TAC'GTA
Cuts at: 493 4116 493
Size: 3623 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6325
SphI G_CATG'C
Cuts at: 1132 3037 3683 1132
Size: 1905 646 3774
Fragments arranged by size:
3774 1905 646
SspI AAT'ATT
Cuts at: 5 52 4249 5
Size: 47 4197 2081
Fragments arranged by size:
4197 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6325
StyI C'CwwG_G
Cuts at: 513 1065 3317 3764 513
Size: 552 2252 447 3074
Fragments arranged by size:
3074 2252 552 447
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2992 4352 4369 4522 4707 5152 325
Size: 1255 1412 1360 17 153 185 445 1498
Fragments arranged by size:
1498 1412 1360 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4571 6283 96
Size: 274 80 33 51 1443 2594 1712 138
Fragments arranged by size:
2594 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6285 6317 6285
Size: 32 6293
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6325
XbaI T'CTAG_A
Cuts at: 3338 3661 3338
Size: 323 6002
XhoI C'TCGA_G
Cuts at: 1943 2394 1943
Size: 451 5874
XmaI C'CCGG_G
Cuts at: 1870 2055 3650 1870
Size: 185 1595 4545
Fragments arranged by size:
4545 1595 185
XmnI GAAnn'nnTTC
Cuts at: 4454 4454
Size: 6325
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4133 276
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI
Bsu36I DraIII EcoRV FalI FseI FspAI NruI PacI
PfoI PmlI PspXI PsrI RsrII SexAI SfiI SrfI
SwaI XcmI