Restriction map for
pFN21AB5939
(Circular) (Six-base) MAPSORT of: pFN21AB5939.seq Check: 2176 from: 1 to: 6229
pFN21AB5939 6229 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4039 278
Size: 53 83 186 936 2503 2468
Fragments arranged by size:
2503 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3572 1937
Size: 1635 4594
Acc65I G'GTAC_C
Cuts at: 3550 3550
Size: 6229
AclI AA'CG_TT
Cuts at: 1563 4356 4729 1563
Size: 2793 373 3063
Fragments arranged by size:
3063 2793 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2234 2302 2411 2945 4292 5676 5944 1632
Size: 602 68 109 534 1347 1384 268 1917
Fragments arranged by size:
1917 1384 1347 602 534 268 109 68
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6032
AflIII A'CryG_T
Cuts at: 1203 2577 5128 6105 1203
Size: 1374 2551 977 1327
Fragments arranged by size:
2551 1374 1327 977
AgeI A'CCGG_T
Cuts at: 5060 5060
Size: 6229
AhdI GACnn_n'nnGTC
Cuts at: 2497 4958 2497
Size: 2461 3768
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6229
AlwNI CAG_nnn'CTG
Cuts at: 3082 3972 5544 3082
Size: 890 1572 3767
Fragments arranged by size:
3767 1572 890
ApaI G_GGCC'C
Cuts at: 2408 2408
Size: 6229
ApaLI G'TGCA_C
Cuts at: 3314 4286 5442 3314
Size: 972 1156 4101
Fragments arranged by size:
4101 1156 972
ApoI r'AATT_y
Cuts at: 1493 3407 3538 3764 3916 6025 1493
Size: 1914 131 226 152 2109 1697
Fragments arranged by size:
2109 1914 1697 226 152 131
AseI AT'TA_AT
Cuts at: 160 1032 4783 160
Size: 872 3751 1606
Fragments arranged by size:
3751 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
AvaI C'yCGr_G
Cuts at: 1870 1943 2063 2909 3455 3554 1870
Size: 73 120 846 546 99 4545
Fragments arranged by size:
4545 846 546 120 99 73
BamHI G'GATC_C
Cuts at: 3559 3926 3559
Size: 367 5862
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3550 5005 618
Size: 325 217 627 1763 1455 1842
Fragments arranged by size:
1842 1763 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2408 3548 1391
Size: 1017 1140 4072
Fragments arranged by size:
4072 1140 1017
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3144 3501 928
Size: 971 1245 357 3656
Fragments arranged by size:
3656 1245 971 357
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4420 4454 1903
Size: 34 50 34 2399 34 3678
Fragments arranged by size:
3678 2399 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4121 5331 4121
Size: 1210 5019
BclI T'GATC_A
Cuts at: 1548 3502 1548
Size: 1954 4275
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4840 136
Size: 107 122 71 631 605 3168 1525
Fragments arranged by size:
3168 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6224 6224
Size: 6229
BlpI GC'TnA_GC
Cuts at: 3646 3646
Size: 6229
Bme1580I G_kGCm'C
Cuts at: 1790 2408 2942 3318 4290 5446 1790
Size: 618 534 376 972 1156 2573
Fragments arranged by size:
2573 1156 972 618 534 376
BmgBI CAC'GTC
Cuts at: 1318 2080 2494 2578 3142 1318
Size: 762 414 84 564 4405
Fragments arranged by size:
4405 762 564 414 84
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3103 3242 3960
Size: 416 53 83 399 24 1686 139 718
Cuts at: 3960 4918 442
Size: 958 1753
Fragments arranged by size:
1753 1686 958 718 416 399 139 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6229
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2521 2521
Size: 6229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2984 3065 3434 4889 1239
Size: 1745 81 369 1455 2579
Fragments arranged by size:
2579 1745 1455 369 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3317 4354 5219 5517 3317
Size: 1037 865 298 4029
Fragments arranged by size:
4029 1037 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4892 882
Size: 617 3393 2219
Fragments arranged by size:
3393 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4020 493
Size: 711 2816 2702
Fragments arranged by size:
2816 2702 711
BsaBI GATnn'nnATC
Cuts at: 3450 6223 3450
Size: 2773 3456
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2058 2253 4036 4418
Size: 53 83 186 936 525 195 1783 382
Cuts at: 4418 5053 275
Size: 635 1451
Fragments arranged by size:
1783 1451 936 635 525 382 195 186
83 53
BsaWI w'CCGG_w
Cuts at: 4662 5060 5334 5481 5892 4662
Size: 398 274 147 411 4999
Fragments arranged by size:
4999 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2567 2597 1178
Size: 30 165 30 1164 30 4810
Fragments arranged by size:
4810 1164 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 2084 2276 2981 2984 3014 3491 1186
Size: 895 3 192 705 3 30 477 3924
Fragments arranged by size:
3924 895 705 477 192 30 3 3
BseYI C'CCAG_C
Cuts at: 2204 2277 5432 5928 2204
Size: 73 3155 496 2505
Fragments arranged by size:
3155 2505 496 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5656
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2946 3679 4440 5468 1540
Size: 275 186 945 733 761 1028 2301
Fragments arranged by size:
2301 1028 945 761 733 275 186
BsiHKAI G_wGCw'C
Cuts at: 3318 4290 4375 5446 3318
Size: 972 85 1071 4101
Fragments arranged by size:
4101 1071 972 85
BsmI GAATG_Cn'
Cuts at: 3740 3833 3740
Size: 93 6136
BsmBI CGTCTCn'nnnn_
Cuts at: 3233 3233
Size: 6229
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2408 2942 3318 3548 4290 4375 5446
Size: 399 618 534 376 230 742 85 1071
Cuts at: 5446 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 618 534 399 376 230
85
BspEI T'CCGG_A
Cuts at: 5892 5892
Size: 6229
BspHI T'CATG_A
Cuts at: 4118 5848 4118
Size: 1730 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2142 2685 3584 844
Size: 1298 543 899 3489
Fragments arranged by size:
3489 1298 899 543
BsrBI CCG'CTC
Cuts at: 1394 1823 3420 4116 1394
Size: 429 1597 696 3507
Fragments arranged by size:
3507 1597 696 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4724 4898 65
Size: 1183 3476 174 1396
Fragments arranged by size:
3476 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4873 5060 1700
Size: 110 3063 187 2869
Fragments arranged by size:
3063 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6229
BssHII G'CGCG_C
Cuts at: 1925 2050 1925
Size: 125 6104
BssSI C'ACGA_G
Cuts at: 4289 5301 4289
Size: 1012 5217
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2521 794
Size: 1727 4502
BstBI TT'CG_AA
Cuts at: 5033 5033
Size: 6229
BstEII G'GTnAC_C
Cuts at: 1184 2662 1184
Size: 1478 4751
BstXI CCAn_nnnn'nTGG
Cuts at: 2467 3298 2467
Size: 831 5398
BstYI r'GATC_y
Cuts at: 1972 2859 3559 3926 4311 4328 5780 5866 5878
Size: 887 700 367 385 17 1452 86 12
Cuts at: 5878 6224 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 887 700 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2034 2256 3188 513
Size: 552 969 222 932 3554
Fragments arranged by size:
3554 969 932 552 222
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3253 487
Size: 683 56 2027 3463
Fragments arranged by size:
3463 2027 683 56
BtsI GCAGTG_nn'
Cuts at: 2167 3199 3745 4539 4559 2167
Size: 1032 546 794 20 3837
Fragments arranged by size:
3837 1032 794 546 20
ClaI AT'CG_AT
Cuts at: 1554 2541 1554
Size: 987 5242
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6194
DraI TTT'AAA
Cuts at: 3533 3533
Size: 6229
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3362 5236 809
Size: 507 2046 1874 1802
Fragments arranged by size:
2046 1874 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2037 2164
Size: 54 1425 50 275 18 201 6 127
Cuts at: 2164 2241 2943 3424 3676 4565 6194 8
Size: 77 702 481 252 889 1629 43
Fragments arranged by size:
1629 1425 889 702 481 275 252 201
127 77 54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2943 3676 1537
Size: 275 1131 733 4090
Fragments arranged by size:
4090 1131 733 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2928 4159 1353
Size: 1575 1231 3423
Fragments arranged by size:
3423 1575 1231
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4816 5190 5336 387
Size: 1064 138 3227 374 146 1280
Fragments arranged by size:
3227 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2302 2411 2945 2984 3065 3434
Size: 393 602 68 109 534 39 81 369
Cuts at: 3434 4292 4889 5676 5944 1239
Size: 858 597 787 268 1524
Fragments arranged by size:
1524 858 787 602 597 534 393 369
268 109 81 68 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2927 2966 2927
Size: 39 6190
EcoO109I rG'GnC_Cy
Cuts at: 3460 3460
Size: 6229
EcoRI G'AATT_C
Cuts at: 3538 3916 3538
Size: 378 5851
FspI TGC'GCA
Cuts at: 4735 4735
Size: 6229
HaeII r_GCGC'y
Cuts at: 1678 2117 5376 1678
Size: 439 3259 2531
Fragments arranged by size:
3259 2531 439
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2483 2515 2567 2599 2745
Size: 32 135 32 911 32 52 32 146
Cuts at: 2745 2777 4870 4902 4944 4976 1373
Size: 32 2093 32 42 32 2626
Fragments arranged by size:
2626 2093 911 146 135 52 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3573 3819 669
Size: 988 281 1635 246 3079
Fragments arranged by size:
3079 1635 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6229
HpaI GTT'AAC
Cuts at: 3819 3819
Size: 6229
KpnI G_GTAC'C
Cuts at: 3554 3554
Size: 6229
MfeI C'AATT_G
Cuts at: 3828 3828
Size: 6229
MluI A'CGCG_T
Cuts at: 6105 6105
Size: 6229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2481 2592 5343 5527 1305
Size: 1176 111 2751 184 2007
Fragments arranged by size:
2751 2007 1176 184 111
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2166 2243 3426 10
Size: 54 1425 343 334 77 1183 2813
Fragments arranged by size:
2813 1425 1183 343 334 77 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4187 4546 4705 518
Size: 648 3021 359 159 2042
Fragments arranged by size:
3021 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2204 2357 2438 2704 3645 4322 5470 5715
Size: 598 153 81 266 941 677 1148 245
Cuts at: 5715 1606
Size: 2120
Fragments arranged by size:
2120 1148 941 677 598 266 245 153
81
NcoI C'CATG_G
Cuts at: 513 1065 3188 513
Size: 552 2123 3554
Fragments arranged by size:
3554 2123 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6229
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6229
NotI GC'GGCC_GC
Cuts at: 3676 3676
Size: 6229
NspI r_CATG'y
Cuts at: 1132 1229 3587 5132 1132
Size: 97 2358 1545 2229
Fragments arranged by size:
2358 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6229
PciI A'CATG_T
Cuts at: 5128 5128
Size: 6229
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2083 3194 1108
Size: 975 1111 4143
Fragments arranged by size:
4143 1111 975
PfoI T'CCnGG_A
Cuts at: 3042 3411 3042
Size: 369 5860
PmeI GTTT'AAAC
Cuts at: 3533 3533
Size: 6229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4298 4330 5811 5843 4298
Size: 32 1481 32 4684
Fragments arranged by size:
4684 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3460 3460
Size: 6229
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6229
PsiI TTA'TAA
Cuts at: 3799 3799
Size: 6229
PspOMI G'GGCC_C
Cuts at: 2404 2404
Size: 6229
PspXI vC'TCGA_Gb
Cuts at: 2909 2909
Size: 6229
PstI C_TGCA'G
Cuts at: 830 2443 3581 830
Size: 1613 1138 3478
Fragments arranged by size:
3478 1613 1138
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6229
PvuII CAG'CTG
Cuts at: 2357 2438 2704 2357
Size: 81 266 5882
Fragments arranged by size:
5882 266 81
SalI G'TCGA_C
Cuts at: 1936 3571 1936
Size: 1635 4594
SbfI CC_TGCA'GG
Cuts at: 3581 3581
Size: 6229
ScaI AGT'ACT
Cuts at: 4477 6189 4477
Size: 1712 4517
SfcI C'TryA_G
Cuts at: 826 1045 2439 3577 4712 5393 5584 826
Size: 219 1394 1138 1135 681 191 1471
Fragments arranged by size:
1471 1394 1138 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6229
SmaI CCC'GGG
Cuts at: 1872 3556 1872
Size: 1684 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2909 3332 3455 4333 5234 5496
Size: 197 926 966 423 123 878 901 262
Cuts at: 5496 820
Size: 1553
Fragments arranged by size:
1553 966 926 901 878 423 262 197
123
SnaBI TAC'GTA
Cuts at: 493 4020 493
Size: 3527 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6229
SphI G_CATG'C
Cuts at: 1132 3587 1132
Size: 2455 3774
SspI AAT'ATT
Cuts at: 5 52 4153 5
Size: 47 4101 2081
Fragments arranged by size:
4101 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6229
StyI C'CwwG_G
Cuts at: 513 1065 2156 2244 2343 2448 2667 3188 3668
Size: 552 1091 88 99 105 219 521 480
Cuts at: 3668 513
Size: 3074
Fragments arranged by size:
3074 1091 552 521 480 219 105 99
88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4256 4273 4426 4611 5056 325
Size: 1255 2676 17 153 185 445 1498
Fragments arranged by size:
2676 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2982 4475 6187
Size: 274 80 33 51 1443 1005 1493 1712
Cuts at: 6187 96
Size: 138
Fragments arranged by size:
1712 1493 1443 1005 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6189 6221 6189
Size: 32 6197
Tth111I GACn'n_nGTC
Cuts at: 1316 2078 1316
Size: 762 5467
XbaI T'CTAG_A
Cuts at: 3565 3565
Size: 6229
XhoI C'TCGA_G
Cuts at: 1943 2909 3455 1943
Size: 966 546 4717
Fragments arranged by size:
4717 966 546
XmaI C'CCGG_G
Cuts at: 1870 3554 1870
Size: 1684 4545
XmnI GAAnn'nnTTC
Cuts at: 4358 4358
Size: 6229
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4037 276
Size: 53 83 186 936 2503 2468
Fragments arranged by size:
2503 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI