Restriction map for
pFN21AB5937
(Circular) (Six-base) MAPSORT of: pFN21AB5937.seq Check: 2529 from: 1 to: 6229
pFN21AB5937 6229 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4039 278
Size: 53 83 186 936 2503 2468
Fragments arranged by size:
2503 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3572 1937
Size: 1635 4594
Acc65I G'GTAC_C
Cuts at: 3550 3550
Size: 6229
AclI AA'CG_TT
Cuts at: 1563 4356 4729 1563
Size: 2793 373 3063
Fragments arranged by size:
3063 2793 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2120 2299 2523 2534 3302 4292 5676 5944
Size: 488 179 224 11 768 990 1384 268
Cuts at: 5944 1632
Size: 1917
Fragments arranged by size:
1917 1384 990 768 488 268 224 179
11
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6032
AflIII A'CryG_T
Cuts at: 1203 3357 5128 6105 1203
Size: 2154 1771 977 1327
Fragments arranged by size:
2154 1771 1327 977
AgeI A'CCGG_T
Cuts at: 5060 5060
Size: 6229
AhdI GACnn_n'nnGTC
Cuts at: 4958 4958
Size: 6229
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6229
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2019 2051 2714 2746 2019
Size: 32 663 32 5502
Fragments arranged by size:
5502 663 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2143 3972 5544 2143
Size: 1829 1572 2828
Fragments arranged by size:
2828 1829 1572
ApaI G_GGCC'C
Cuts at: 2373 2373
Size: 6229
ApaLI G'TGCA_C
Cuts at: 4286 5442 4286
Size: 1156 5073
ApoI r'AATT_y
Cuts at: 1493 3538 3764 3916 6025 1493
Size: 2045 226 152 2109 1697
Fragments arranged by size:
2109 2045 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4783 160
Size: 872 3751 1606
Fragments arranged by size:
3751 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
AvaI C'yCGr_G
Cuts at: 1870 1943 2217 2373 3307 3350 3554 1870
Size: 73 274 156 934 43 204 4545
Fragments arranged by size:
4545 934 274 204 156 73 43
BamHI G'GATC_C
Cuts at: 3559 3926 3559
Size: 367 5862
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2120 3550 5005 618
Size: 325 217 627 333 1430 1455 1842
Fragments arranged by size:
1842 1455 1430 627 333 325 217
BanII G_rGCy'C
Cuts at: 1391 2373 2585 2768 3249 3548 1391
Size: 982 212 183 481 299 4072
Fragments arranged by size:
4072 982 481 299 212 183
BbeI G_GCGC'C
Cuts at: 2124 2124
Size: 6229
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2522 3307 928
Size: 971 623 785 3850
Fragments arranged by size:
3850 971 785 623
BbvCI CC'TCA_GC
Cuts at: 2480 2480
Size: 6229
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2195 2229 4420 4454 1903
Size: 34 258 34 2191 34 3678
Fragments arranged by size:
3678 2191 258 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4121 5331 4121
Size: 1210 5019
BclI T'GATC_A
Cuts at: 1548 3402 1548
Size: 1854 4375
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2081 3343 4840
Size: 107 122 71 631 605 409 1262 1497
Cuts at: 4840 136
Size: 1525
Fragments arranged by size:
1525 1497 1262 631 605 409 122 107
71
BglII A'GATC_T
Cuts at: 2712 6224 2712
Size: 3512 2717
BlpI GC'TnA_GC
Cuts at: 2088 2579 3646 2088
Size: 491 1067 4671
Fragments arranged by size:
4671 1067 491
Bme1580I G_kGCm'C
Cuts at: 1790 2373 4290 5446 1790
Size: 583 1917 1156 2573
Fragments arranged by size:
2573 1917 1156 583
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6229
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2194 2292 2533
Size: 416 53 83 399 24 777 98 241
Cuts at: 2533 2695 3960 4918 442
Size: 162 1265 958 1753
Fragments arranged by size:
1753 1265 958 777 416 399 241 162
98 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6229
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2611 3525 2611
Size: 914 5315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2066 2408 2581 2741 3407 4889 1239
Size: 827 342 173 160 666 1482 2579
Fragments arranged by size:
2579 1482 827 666 342 173 160
Bpu10I CC'TnA_GC
Cuts at: 2480 2480
Size: 6229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2672 4354 5219 5517 2672
Size: 1682 865 298 3384
Fragments arranged by size:
3384 1682 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3077 3152 4892 882
Size: 617 1578 75 1740 2219
Fragments arranged by size:
2219 1740 1578 617 75
BsaAI yAC'GTr
Cuts at: 493 1204 4020 493
Size: 711 2816 2702
Fragments arranged by size:
2816 2702 711
BsaBI GATnn'nnATC
Cuts at: 6223 6223
Size: 6229
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2051 2121 2784 3093
Size: 53 83 186 936 518 70 663 309
Cuts at: 3093 4036 4418 5053 275
Size: 943 382 635 1451
Fragments arranged by size:
1451 943 936 663 635 518 382 309
186 83 70 53
BsaWI w'CCGG_w
Cuts at: 3043 3075 4662 5060 5334 5481 5892 3043
Size: 32 1587 398 274 147 411 3380
Fragments arranged by size:
3380 1587 411 398 274 147 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2019 2049 2714 2744 1178
Size: 30 165 30 616 30 665 30 4663
Fragments arranged by size:
4663 665 616 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2222 2485 1186
Size: 1036 263 4930
Fragments arranged by size:
4930 1036 263
BseYI C'CCAG_C
Cuts at: 2092 2143 2455 2799 2809 3196 3248 5432 5928
Size: 51 312 344 10 387 52 2184 496
Cuts at: 5928 2092
Size: 2393
Fragments arranged by size:
2393 2184 496 387 344 312 52 51
10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2432 1163
Size: 573 696 4960
Fragments arranged by size:
4960 696 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3067 3679 4440 5468 1540
Size: 275 186 1066 612 761 1028 2301
Fragments arranged by size:
2301 1066 1028 761 612 275 186
BsiHKAI G_wGCw'C
Cuts at: 2106 2768 4290 4375 5446 2106
Size: 662 1522 85 1071 2889
Fragments arranged by size:
2889 1522 1071 662 85
BsmI GAATG_Cn'
Cuts at: 2253 3740 3833 2253
Size: 1487 93 4649
Fragments arranged by size:
4649 1487 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2373 2585 2768 3249 3548 4290
Size: 399 316 267 212 183 481 299 742
Cuts at: 4290 4375 5446 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 481 399 316 299 267
212 183 85
BspEI T'CCGG_A
Cuts at: 3075 5892 3075
Size: 2817 3412
BspHI T'CATG_A
Cuts at: 4118 5848 4118
Size: 1730 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3462 3584 844
Size: 2618 122 3489
Fragments arranged by size:
3489 2618 122
BsrBI CCG'CTC
Cuts at: 1394 1823 2697 4116 1394
Size: 429 874 1419 3507
Fragments arranged by size:
3507 1419 874 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3366 4724 4898 65
Size: 1183 2118 1358 174 1396
Fragments arranged by size:
2118 1396 1358 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2616 4873 5060 1700
Size: 110 806 2257 187 2869
Fragments arranged by size:
2869 2257 806 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6229
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6229
BssSI C'ACGA_G
Cuts at: 4289 5301 4289
Size: 1012 5217
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6229
BstBI TT'CG_AA
Cuts at: 2248 5033 2248
Size: 2785 3444
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6229
BstXI CCAn_nnnn'nTGG
Cuts at: 3103 3103
Size: 6229
BstYI r'GATC_y
Cuts at: 1972 2712 2845 3559 3926 4311 4328 5780 5866
Size: 740 133 714 367 385 17 1452 86
Cuts at: 5866 5878 6224 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 740 714 385 367 346 133
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2647 3339 513
Size: 552 939 643 692 3403
Fragments arranged by size:
3403 939 692 643 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5490
Fragments arranged by size:
5490 683 56
BtsI GCAGTG_nn'
Cuts at: 2545 3745 4539 4559 2545
Size: 1200 794 20 4215
Fragments arranged by size:
4215 1200 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6194
DraI TTT'AAA
Cuts at: 3533 3533
Size: 6229
DraIII CAC_nnn'GTG
Cuts at: 3448 3448
Size: 6229
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5236 809
Size: 507 3920 1802
Fragments arranged by size:
3920 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2668 3676 4565
Size: 54 1425 50 275 18 838 1008 889
Cuts at: 4565 6194 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1008 889 838 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3676 1537
Size: 275 1864 4090
Fragments arranged by size:
4090 1864 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2123 2480 2795 4159 1353
Size: 770 357 315 1364 3423
Fragments arranged by size:
3423 1364 770 357 315
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2039 3152 4816 5190 5336
Size: 1064 138 435 15 1113 1664 374 146
Cuts at: 5336 387
Size: 1280
Fragments arranged by size:
1664 1280 1113 1064 435 374 146 138
15
EcoICRI GAG'CTC
Cuts at: 2766 2766
Size: 6229
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2120 2299 2408 2523 2534 2581
Size: 393 434 54 179 109 115 11 47
Cuts at: 2581 2741 3302 3407 4292 4889 5676 5944 1239
Size: 160 561 105 885 597 787 268 1524
Fragments arranged by size:
1524 885 787 597 561 434 393 268
179 160 115 109 105 54 47 11
EcoO109I rG'GnC_Cy
Cuts at: 2266 2370 2857 3376 3504 2266
Size: 104 487 519 128 4991
Fragments arranged by size:
4991 519 487 128 104
EcoRI G'AATT_C
Cuts at: 3538 3916 3538
Size: 378 5851
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3052 3052
Size: 6229
FspI TGC'GCA
Cuts at: 4735 4735
Size: 6229
HaeII r_GCGC'y
Cuts at: 1678 2076 2124 2144 3003 3394 5376 1678
Size: 398 48 20 859 391 1982 2531
Fragments arranged by size:
2531 1982 859 398 391 48 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2890 2922 3344 3376 4870
Size: 32 135 32 1318 32 422 32 1494
Cuts at: 4870 4902 4944 4976 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1494 1318 422 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3573 3819 669
Size: 988 281 1635 246 3079
Fragments arranged by size:
3079 1635 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6229
HpaI GTT'AAC
Cuts at: 3819 3819
Size: 6229
KasI G'GCGC_C
Cuts at: 2120 2120
Size: 6229
KpnI G_GTAC'C
Cuts at: 3554 3554
Size: 6229
MfeI C'AATT_G
Cuts at: 3828 3828
Size: 6229
MluI A'CGCG_T
Cuts at: 6105 6105
Size: 6229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2355 2731 3280 5343 5527 1305
Size: 1050 376 549 2063 184 2007
Fragments arranged by size:
2063 2007 1050 549 376 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4407
Fragments arranged by size:
4407 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2738 3257 4187 4546 4705 518
Size: 648 1572 519 930 359 159 2042
Fragments arranged by size:
2042 1572 930 648 519 359 159
MspA1I CmG'CkG
Cuts at: 1606 2416 2428 2667 2683 2852 2887 3645 4322
Size: 810 12 239 16 169 35 758 677
Cuts at: 4322 5470 5715 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 810 758 677 245 239 169
35 16 12
NarI GG'CG_CC
Cuts at: 2121 2121
Size: 6229
NcoI C'CATG_G
Cuts at: 513 1065 2004 3339 513
Size: 552 939 1335 3403
Fragments arranged by size:
3403 1335 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6229
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6229
NotI GC'GGCC_GC
Cuts at: 3676 3676
Size: 6229
NspI r_CATG'y
Cuts at: 1132 1229 3587 5132 1132
Size: 97 2358 1545 2229
Fragments arranged by size:
2358 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6229
PciI A'CATG_T
Cuts at: 5128 5128
Size: 6229
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2113 2184 2337 3166 1108
Size: 1005 71 153 829 4171
Fragments arranged by size:
4171 1005 829 153 71
PfoI T'CCnGG_A
Cuts at: 2718 3426 2718
Size: 708 5521
PmeI GTTT'AAAC
Cuts at: 3533 3533
Size: 6229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2019 2051 2714 2746 4298 4330 5811 5843 2019
Size: 32 663 32 1552 32 1481 32 2405
Fragments arranged by size:
2405 1552 1481 663 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6229
PsiI TTA'TAA
Cuts at: 3799 3799
Size: 6229
PspOMI G'GGCC_C
Cuts at: 2369 2369
Size: 6229
PstI C_TGCA'G
Cuts at: 830 2865 3459 3581 830
Size: 2035 594 122 3478
Fragments arranged by size:
3478 2035 594 122
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6229
PvuII CAG'CTG
Cuts at: 2428 2428
Size: 6229
SacI G_AGCT'C
Cuts at: 2768 2768
Size: 6229
SalI G'TCGA_C
Cuts at: 1936 3571 1936
Size: 1635 4594
SapI GCTCTTCn'nnn_
Cuts at: 2123 2123
Size: 6229
SbfI CC_TGCA'GG
Cuts at: 3581 3581
Size: 6229
ScaI AGT'ACT
Cuts at: 3053 4477 6189 3053
Size: 1424 1712 3093
Fragments arranged by size:
3093 1712 1424
SfcI C'TryA_G
Cuts at: 826 1045 2861 3455 3577 4712 5393 5584 826
Size: 219 1816 594 122 1135 681 191 1471
Fragments arranged by size:
1816 1471 1135 681 594 219 191 122
SfoI GGC'GCC
Cuts at: 2122 2122
Size: 6229
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6229
SmaI CCC'GGG
Cuts at: 1872 2219 2375 3556 1872
Size: 347 156 1181 4545
Fragments arranged by size:
4545 1181 347 156
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2687 4333 5234 5496 820
Size: 197 926 744 1646 901 262 1553
Fragments arranged by size:
1646 1553 926 901 744 262 197
SnaBI TAC'GTA
Cuts at: 493 4020 493
Size: 3527 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6229
SphI G_CATG'C
Cuts at: 1132 3587 1132
Size: 2455 3774
SspI AAT'ATT
Cuts at: 5 52 4153 5
Size: 47 4101 2081
Fragments arranged by size:
4101 2081 47
StuI AGG'CCT
Cuts at: 1517 3279 1517
Size: 1762 4467
StyI C'CwwG_G
Cuts at: 513 1065 2004 3153 3274 3339 3668 513
Size: 552 939 1149 121 65 329 3074
Fragments arranged by size:
3074 1149 939 552 329 121 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2160 2515 2823 3280 3352 4256 4273
Size: 1255 580 355 308 457 72 904 17
Cuts at: 4273 4426 4611 5056 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 904 580 457 445 355 308
185 153 72 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3051 4475 6187
Size: 274 80 33 51 1443 1074 1424 1712
Cuts at: 6187 96
Size: 138
Fragments arranged by size:
1712 1443 1424 1074 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6189 6221 6189
Size: 32 6197
Tth111I GACn'n_nGTC
Cuts at: 1316 2879 1316
Size: 1563 4666
XbaI T'CTAG_A
Cuts at: 3565 3565
Size: 6229
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2305 2305
Size: 6229
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6229
XmaI C'CCGG_G
Cuts at: 1870 2217 2373 3554 1870
Size: 347 156 1181 4545
Fragments arranged by size:
4545 1181 347 156
XmnI GAAnn'nnTTC
Cuts at: 3035 4358 3035
Size: 1323 4906
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4037 276
Size: 53 83 186 936 2503 2468
Fragments arranged by size:
2503 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SrfI SwaI