Restriction map for
pFN21AB5935
(Circular) (Six-base) MAPSORT of: pFN21AB5935.seq Check: 451 from: 1 to: 6229
pFN21AB5935 6229 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4039 278
Size: 53 83 186 936 2503 2468
Fragments arranged by size:
2503 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3572 1937
Size: 1635 4594
Acc65I G'GTAC_C
Cuts at: 3550 3550
Size: 6229
AclI AA'CG_TT
Cuts at: 1563 4356 4729 1563
Size: 2793 373 3063
Fragments arranged by size:
3063 2793 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2517 2763 4292 5676 5944 1632
Size: 885 246 1529 1384 268 1917
Fragments arranged by size:
1917 1529 1384 885 268 246
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6032
AflIII A'CryG_T
Cuts at: 1203 2301 5128 6105 1203
Size: 1098 2827 977 1327
Fragments arranged by size:
2827 1327 1098 977
AgeI A'CCGG_T
Cuts at: 5060 5060
Size: 6229
AhdI GACnn_n'nnGTC
Cuts at: 4958 4958
Size: 6229
AleI CACnn'nnGTG
Cuts at: 1166 2636 1166
Size: 1470 4759
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2654 1184
Size: 1470 4759
AlwNI CAG_nnn'CTG
Cuts at: 3972 5544 3972
Size: 1572 4657
ApaLI G'TGCA_C
Cuts at: 4286 5442 4286
Size: 1156 5073
ApoI r'AATT_y
Cuts at: 1493 2753 3454 3538 3764 3916 6025 1493
Size: 1260 701 84 226 152 2109 1697
Fragments arranged by size:
2109 1697 1260 701 226 152 84
AseI AT'TA_AT
Cuts at: 160 1032 2076 4783 160
Size: 872 1044 2707 1606
Fragments arranged by size:
2707 1606 1044 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
AvaI C'yCGr_G
Cuts at: 1870 1943 3554 1870
Size: 73 1611 4545
Fragments arranged by size:
4545 1611 73
BamHI G'GATC_C
Cuts at: 2719 3559 3926 2719
Size: 840 367 5022
Fragments arranged by size:
5022 840 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3550 5005 618
Size: 325 217 627 1763 1455 1842
Fragments arranged by size:
1842 1763 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3548 1391
Size: 2157 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5258
BbvCI CC'TCA_GC
Cuts at: 2571 2571
Size: 6229
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3422 3456 4420 4454 1903
Size: 34 1485 34 964 34 3678
Fragments arranged by size:
3678 1485 964 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4121 5331 4121
Size: 1210 5019
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6229
BfrBI ATG'CAT
Cuts at: 3172 3172
Size: 6229
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4840 136
Size: 107 122 71 631 605 3168 1525
Fragments arranged by size:
3168 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6224 6224
Size: 6229
BlpI GC'TnA_GC
Cuts at: 3646 3646
Size: 6229
Bme1580I G_kGCm'C
Cuts at: 1790 2281 4290 5446 1790
Size: 491 2009 1156 2573
Fragments arranged by size:
2573 2009 1156 491
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6229
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3960 4918 442
Size: 416 53 83 399 24 2543 958 1753
Fragments arranged by size:
2543 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2403 2828 4889 1239
Size: 1164 425 2061 2579
Fragments arranged by size:
2579 2061 1164 425
Bpu10I CC'TnA_GC
Cuts at: 2571 2571
Size: 6229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4354 5219 5517 4354
Size: 865 298 5066
Fragments arranged by size:
5066 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4892 882
Size: 617 3393 2219
Fragments arranged by size:
3393 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4020 493
Size: 711 2816 2702
Fragments arranged by size:
2816 2702 711
BsaBI GATnn'nnATC
Cuts at: 2052 3428 6223 2052
Size: 1376 2795 2058
Fragments arranged by size:
2795 2058 1376
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3238 3280 4036 4418
Size: 53 83 186 936 1705 42 756 382
Cuts at: 4418 5053 275
Size: 635 1451
Fragments arranged by size:
1705 1451 936 756 635 382 186 83
53 42
BsaWI w'CCGG_w
Cuts at: 4662 5060 5334 5481 5892 4662
Size: 398 274 147 411 4999
Fragments arranged by size:
4999 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2959 2989 1178
Size: 30 165 30 1556 30 4418
Fragments arranged by size:
4418 1556 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6229
BseYI C'CCAG_C
Cuts at: 5432 5928 5432
Size: 496 5733
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2304 1163
Size: 573 568 5088
Fragments arranged by size:
5088 573 568
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3679 4440 5468 1540
Size: 275 186 1678 761 1028 2301
Fragments arranged by size:
2301 1678 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2524 3217 4290 4375 5446 2524
Size: 693 1073 85 1071 3307
Fragments arranged by size:
3307 1073 1071 693 85
BsmI GAATG_Cn'
Cuts at: 3740 3833 3740
Size: 93 6136
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2281 2524 3217 3548 4290 4375 5446
Size: 399 491 243 693 331 742 85 1071
Cuts at: 5446 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 693 491 399 331 243
85
BspEI T'CCGG_A
Cuts at: 5892 5892
Size: 6229
BspHI T'CATG_A
Cuts at: 4118 5848 4118
Size: 1730 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3584 844
Size: 2740 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2620 4116 1394
Size: 429 797 1496 3507
Fragments arranged by size:
3507 1496 797 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4724 4898 65
Size: 1183 3476 174 1396
Fragments arranged by size:
3476 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4873 5060 1700
Size: 110 3063 187 2869
Fragments arranged by size:
3063 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3248 96
Size: 3152 3077
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6229
BssSI C'ACGA_G
Cuts at: 3474 4289 5301 3474
Size: 815 1012 4402
Fragments arranged by size:
4402 1012 815
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6229
BstBI TT'CG_AA
Cuts at: 5033 5033
Size: 6229
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6229
BstYI r'GATC_y
Cuts at: 1972 2719 3163 3559 3926 4311 4328 5780 5866
Size: 747 444 396 367 385 17 1452 86
Cuts at: 5866 5878 6224 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 747 444 396 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2956 2956
Size: 6229
BtgI C'CryG_G
Cuts at: 513 1065 2004 3525 513
Size: 552 939 1521 3217
Fragments arranged by size:
3217 1521 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5490
Fragments arranged by size:
5490 683 56
BtsI GCAGTG_nn'
Cuts at: 3343 3467 3745 4539 4559 3343
Size: 124 278 794 20 5013
Fragments arranged by size:
5013 794 278 124 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6194
DraI TTT'AAA
Cuts at: 3533 3533
Size: 6229
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5236 809
Size: 507 3920 1802
Fragments arranged by size:
3920 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3676 4565 6194
Size: 54 1425 50 275 18 1846 889 1629
Cuts at: 6194 8
Size: 43
Fragments arranged by size:
1846 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3676 1537
Size: 275 1864 4090
Fragments arranged by size:
4090 1864 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2340 2610 2815 2884 4159 1353
Size: 987 270 205 69 1275 3423
Fragments arranged by size:
3423 1275 987 270 205 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4816 5190 5336 387
Size: 1064 138 3227 374 146 1280
Fragments arranged by size:
3227 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2403 2517 2763 2828 4292 4889 5676
Size: 393 771 114 246 65 1464 597 787
Cuts at: 5676 5944 1239
Size: 268 1524
Fragments arranged by size:
1524 1464 787 771 597 393 268 246
114 65
EcoNI CCTnn'n_nnAGG
Cuts at: 2535 2535
Size: 6229
EcoRI G'AATT_C
Cuts at: 3538 3916 3538
Size: 378 5851
EcoRV GAT'ATC
Cuts at: 2453 2462 3274 2453
Size: 9 812 5408
Fragments arranged by size:
5408 812 9
FspI TGC'GCA
Cuts at: 4735 4735
Size: 6229
HaeII r_GCGC'y
Cuts at: 1678 5376 1678
Size: 3698 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2244 2276 2468 2500 2885
Size: 32 135 32 672 32 192 32 385
Cuts at: 2885 2917 2959 2991 3121 3153 3473 3505 4870
Size: 32 42 32 130 32 320 32 1365
Cuts at: 4870 4902 4944 4976 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1365 672 385 320 192 135 130
42 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2596 2908 3322 3573 3819 669
Size: 988 281 658 312 414 251 246 3079
Fragments arranged by size:
3079 988 658 414 312 281 251 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6229
HpaI GTT'AAC
Cuts at: 2908 3819 2908
Size: 911 5318
KpnI G_GTAC'C
Cuts at: 3554 3554
Size: 6229
MfeI C'AATT_G
Cuts at: 3828 3828
Size: 6229
MluI A'CGCG_T
Cuts at: 6105 6105
Size: 6229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5343 5527 1305
Size: 4038 184 2007
Fragments arranged by size:
4038 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4407
Fragments arranged by size:
4407 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2078 2589 2636 3177 4187 4546 4705
Size: 648 912 511 47 541 1010 359 159
Cuts at: 4705 518
Size: 2042
Fragments arranged by size:
2042 1010 912 648 541 511 359 159
47
MspA1I CmG'CkG
Cuts at: 1606 3645 4322 5470 5715 1606
Size: 2039 677 1148 245 2120
Fragments arranged by size:
2120 2039 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3525 513
Size: 552 939 1521 3217
Fragments arranged by size:
3217 1521 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6229
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6229
NotI GC'GGCC_GC
Cuts at: 3676 3676
Size: 6229
NsiI A_TGCA'T
Cuts at: 3174 3174
Size: 6229
NspI r_CATG'y
Cuts at: 1132 1229 2305 2645 3587 5132 1132
Size: 97 1076 340 942 1545 2229
Fragments arranged by size:
2229 1545 1076 942 340 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6229
PciI A'CATG_T
Cuts at: 2301 5128 2301
Size: 2827 3402
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6229
PfoI T'CCnGG_A
Cuts at: 2419 2419
Size: 6229
PmeI GTTT'AAAC
Cuts at: 3533 3533
Size: 6229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4298 4330 5811 5843 4298
Size: 32 1481 32 4684
Fragments arranged by size:
4684 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6229
PsiI TTA'TAA
Cuts at: 3799 3799
Size: 6229
PstI C_TGCA'G
Cuts at: 830 3581 830
Size: 2751 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6229
SalI G'TCGA_C
Cuts at: 1936 3571 1936
Size: 1635 4594
SapI GCTCTTCn'nnn_
Cuts at: 2340 2610 2340
Size: 270 5959
SbfI CC_TGCA'GG
Cuts at: 3581 3581
Size: 6229
ScaI AGT'ACT
Cuts at: 3514 4477 6189 3514
Size: 963 1712 3554
Fragments arranged by size:
3554 1712 963
SfcI C'TryA_G
Cuts at: 826 1045 2601 3577 4712 5393 5584 826
Size: 219 1556 976 1135 681 191 1471
Fragments arranged by size:
1556 1471 1135 976 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6229
SmaI CCC'GGG
Cuts at: 1872 3556 1872
Size: 1684 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4333 5234 5496 820
Size: 197 926 2390 901 262 1553
Fragments arranged by size:
2390 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4020 493
Size: 3527 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6229
SphI G_CATG'C
Cuts at: 1132 3587 1132
Size: 2455 3774
SspI AAT'ATT
Cuts at: 5 52 3230 4153 5
Size: 47 3178 923 2081
Fragments arranged by size:
3178 2081 923 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6229
StyI C'CwwG_G
Cuts at: 513 1065 2004 3525 3668 513
Size: 552 939 1521 143 3074
Fragments arranged by size:
3074 1521 939 552 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4256 4273 4426 4611 5056 325
Size: 1255 2676 17 153 185 445 1498
Fragments arranged by size:
2676 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2581 3248 3512
Size: 274 80 33 51 1443 604 667 264
Cuts at: 3512 4475 6187 96
Size: 963 1712 138
Fragments arranged by size:
1712 1443 963 667 604 274 264 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6189 6221 6189
Size: 32 6197
Tth111I GACn'n_nGTC
Cuts at: 1316 2039 1316
Size: 723 5506
XbaI T'CTAG_A
Cuts at: 3160 3565 3160
Size: 405 5824
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6229
XmaI C'CCGG_G
Cuts at: 1870 3554 1870
Size: 1684 4545
XmnI GAAnn'nnTTC
Cuts at: 4358 4358
Size: 6229
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4037 276
Size: 53 83 186 936 2503 2468
Fragments arranged by size:
2503 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI