Restriction map for
pFN21AB5930
(Circular) (Six-base) MAPSORT of: pFN21AB5930.seq Check: 6864 from: 1 to: 6319
pFN21AB5930 6319 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2252 2590 2724 2252
Size: 338 134 5847
Fragments arranged by size:
5847 338 134
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4129 278
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3662 1937
Size: 1725 4594
Acc65I G'GTAC_C
Cuts at: 3640 3640
Size: 6319
AclI AA'CG_TT
Cuts at: 1563 4446 4819 1563
Size: 2883 373 3063
Fragments arranged by size:
3063 2883 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2198 2450 2474 2789 4382 5766 6034 1632
Size: 566 252 24 315 1593 1384 268 1917
Fragments arranged by size:
1917 1593 1384 566 315 268 252 24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6122
AflIII A'CryG_T
Cuts at: 1203 3468 5218 6195 1203
Size: 2265 1750 977 1327
Fragments arranged by size:
2265 1750 1327 977
AgeI A'CCGG_T
Cuts at: 5150 5150
Size: 6319
AhdI GACnn_n'nnGTC
Cuts at: 5048 5048
Size: 6319
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6319
AlwNI CAG_nnn'CTG
Cuts at: 3057 3143 4062 5634 3057
Size: 86 919 1572 3742
Fragments arranged by size:
3742 1572 919 86
ApaI G_GGCC'C
Cuts at: 2616 3327 2616
Size: 711 5608
ApaLI G'TGCA_C
Cuts at: 2883 4376 5532 2883
Size: 1493 1156 3670
Fragments arranged by size:
3670 1493 1156
ApoI r'AATT_y
Cuts at: 1493 3628 3854 4006 6115 1493
Size: 2135 226 152 2109 1697
Fragments arranged by size:
2135 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4873 160
Size: 872 3841 1606
Fragments arranged by size:
3841 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
AvaI C'yCGr_G
Cuts at: 1870 1943 2584 3076 3533 3644 1870
Size: 73 641 492 457 111 4545
Fragments arranged by size:
4545 641 492 457 111 73
BamHI G'GATC_C
Cuts at: 3649 4016 3649
Size: 367 5952
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2932 3640 5095 618
Size: 325 217 627 1145 708 1455 1842
Fragments arranged by size:
1842 1455 1145 708 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2616 2927 2978 3086 3234 3327 3443 3494
Size: 1225 311 51 108 148 93 116 51
Cuts at: 3494 3602 3638 1391
Size: 108 36 4072
Fragments arranged by size:
4072 1225 311 148 116 108 108 93
51 51 36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2439 2737 2777 928
Size: 971 540 298 40 4470
Fragments arranged by size:
4470 971 540 298 40
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2420 2454 4510 4544 1903
Size: 34 50 34 399 34 2056 34 3678
Fragments arranged by size:
3678 2056 399 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4211 5421 4211
Size: 1210 5109
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6319
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4930 136
Size: 107 122 71 631 605 3258 1525
Fragments arranged by size:
3258 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6314 6314
Size: 6319
BlpI GC'TnA_GC
Cuts at: 3085 3736 3085
Size: 651 5668
Bme1580I G_kGCm'C
Cuts at: 1790 2616 2887 2937 3045 3327 4380 5536 1790
Size: 826 271 50 108 282 1053 1156 2573
Fragments arranged by size:
2573 1156 1053 826 282 271 108 50
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6319
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2674 3388 4050
Size: 416 53 83 399 24 1257 714 662
Cuts at: 4050 5008 442
Size: 958 1753
Fragments arranged by size:
1753 1257 958 714 662 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6319
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2209 2953 3457 2209
Size: 744 504 5071
Fragments arranged by size:
5071 744 504
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2030 2078 2588 2665 2782 2884 2927 2941
Size: 791 48 510 77 117 102 43 14
Cuts at: 2941 2966 3248 3435 4979 1239
Size: 25 282 187 1544 2579
Fragments arranged by size:
2579 1544 791 510 282 187 117 102
77 48 43 25 14
Bpu10I CC'TnA_GC
Cuts at: 2823 2823
Size: 6319
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2527 4444 5309 5607 2527
Size: 1917 865 298 3239
Fragments arranged by size:
3239 1917 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2490 3427 4982 882
Size: 617 991 937 1555 2219
Fragments arranged by size:
2219 1555 991 937 617
BsaAI yAC'GTr
Cuts at: 493 1204 4110 493
Size: 711 2906 2702
Fragments arranged by size:
2906 2702 711
BsaBI GATnn'nnATC
Cuts at: 6313 6313
Size: 6319
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4126 4508 5143 275
Size: 53 83 186 936 2593 382 635 1451
Fragments arranged by size:
2593 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4752 5150 5424 5571 5982 4752
Size: 398 274 147 411 5089
Fragments arranged by size:
5089 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6094
Fragments arranged by size:
6094 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2533 2549 2617 2702 2717 3113 1186
Size: 1347 16 68 85 15 396 4392
Fragments arranged by size:
4392 1347 396 85 68 16 15
BseYI C'CCAG_C
Cuts at: 2016 2055 2135 2816 2911 3050 3202 3233 3383
Size: 39 80 681 95 139 152 31 150
Cuts at: 3383 5522 6018 2016
Size: 2139 496 2317
Fragments arranged by size:
2317 2139 681 496 152 150 139 95
80 39 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5746
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3769 4530 5558 1540
Size: 275 186 1768 761 1028 2301
Fragments arranged by size:
2301 1768 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2887 4380 4465 5536 2887
Size: 1493 85 1071 3670
Fragments arranged by size:
3670 1493 1071 85
BsmI GAATG_Cn'
Cuts at: 3830 3923 3830
Size: 93 6226
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2616 2887 2927 2937 2978 3045 3086
Size: 399 826 271 40 10 41 67 41
Cuts at: 3086 3234 3327 3443 3494 3602 3638 4380 4465
Size: 148 93 116 51 108 36 742 85
Cuts at: 4465 5536 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 826 742 399 271 148 116
108 93 85 67 51 41 41 40
36 10
BspEI T'CCGG_A
Cuts at: 5982 5982
Size: 6319
BspHI T'CATG_A
Cuts at: 4208 5938 4208
Size: 1730 4589
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2252 2590 2724 3674 844
Size: 1408 338 134 950 3489
Fragments arranged by size:
3489 1408 950 338 134
BsrBI CCG'CTC
Cuts at: 1394 1823 2827 4206 1394
Size: 429 1004 1379 3507
Fragments arranged by size:
3507 1379 1004 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4814 4988 65
Size: 1183 3566 174 1396
Fragments arranged by size:
3566 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2362 3405 3452 4963 5150 1700
Size: 110 552 1043 47 1511 187 2869
Fragments arranged by size:
2869 1511 1043 552 187 110 47
BsrGI T'GTAC_A
Cuts at: 96 2844 2952 96
Size: 2748 108 3463
Fragments arranged by size:
3463 2748 108
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6319
BssSI C'ACGA_G
Cuts at: 4379 5391 4379
Size: 1012 5307
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6319
BstBI TT'CG_AA
Cuts at: 5123 5123
Size: 6319
BstEII G'GTnAC_C
Cuts at: 1184 3408 1184
Size: 2224 4095
BstXI CCAn_nnnn'nTGG
Cuts at: 2330 3171 2330
Size: 841 5478
BstYI r'GATC_y
Cuts at: 1972 3649 4016 4401 4418 5870 5956 5968 6314
Size: 1677 367 385 17 1452 86 12 346
Cuts at: 6314 1972
Size: 1977
Fragments arranged by size:
1977 1677 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2969 513
Size: 552 1904 3863
Fragments arranged by size:
3863 1904 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5580
Fragments arranged by size:
5580 683 56
BtsI GCAGTG_nn'
Cuts at: 3835 4629 4649 3835
Size: 794 20 5505
Fragments arranged by size:
5505 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6319
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6284
DraI TTT'AAA
Cuts at: 3623 3623
Size: 6319
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5326 809
Size: 507 4010 1802
Fragments arranged by size:
4010 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2271 2979 3059
Size: 54 1425 50 275 18 441 708 80
Cuts at: 3059 3110 3403 3450 3766 4655 6284 8
Size: 51 293 47 316 889 1629 43
Fragments arranged by size:
1629 1425 889 708 441 316 293 275
80 54 51 50 47 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3766 1537
Size: 275 1954 4090
Fragments arranged by size:
4090 1954 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2166 4249 1353
Size: 813 2083 3423
Fragments arranged by size:
3423 2083 813
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3554 3576 4906 5280 5426 387
Size: 1064 138 1965 22 1330 374 146 1280
Fragments arranged by size:
1965 1330 1280 1064 374 146 138 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2078 2198 2450 2474 2588 2665
Size: 393 398 48 120 252 24 114 77
Cuts at: 2665 2782 2789 2884 2927 2941 2966 3248 3435
Size: 117 7 95 43 14 25 282 187
Cuts at: 3435 4382 4979 5766 6034 1239
Size: 947 597 787 268 1524
Fragments arranged by size:
1524 947 787 597 398 393 282 268
252 187 120 117 114 95 77 48
43 25 24 14 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2846 3315 2846
Size: 469 5850
EcoO109I rG'GnC_Cy
Cuts at: 2612 2640 2915 3250 3323 3324 3545 2612
Size: 28 275 335 73 1 221 5386
Fragments arranged by size:
5386 335 275 221 73 28 1
EcoRI G'AATT_C
Cuts at: 3628 4006 3628
Size: 378 5941
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3070 3070
Size: 6319
FseI GG_CCGG'CC
Cuts at: 3456 3456
Size: 6319
FspI TGC'GCA
Cuts at: 4825 4825
Size: 6319
HaeII r_GCGC'y
Cuts at: 1678 2422 5466 1678
Size: 744 3044 2531
Fragments arranged by size:
3044 2531 744
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4960 4992 5034 5066 1373
Size: 32 135 32 3388 32 42 32 2626
Fragments arranged by size:
3388 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2293 3663 3909 669
Size: 988 281 355 1370 246 3079
Fragments arranged by size:
3079 1370 988 355 281 246
HindIII A'AGCT_T
Cuts at: 748 2462 748
Size: 1714 4605
HpaI GTT'AAC
Cuts at: 3909 3909
Size: 6319
KpnI G_GTAC'C
Cuts at: 3644 3644
Size: 6319
MfeI C'AATT_G
Cuts at: 3918 3918
Size: 6319
MluI A'CGCG_T
Cuts at: 6195 6195
Size: 6319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5433 5617 1305
Size: 4128 184 2007
Fragments arranged by size:
4128 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4497
Fragments arranged by size:
4497 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2253 4277 4636 4795 518
Size: 648 1087 2024 359 159 2042
Fragments arranged by size:
2042 2024 1087 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2859 2958 3183 3302 3735 4412 5560 5805
Size: 1253 99 225 119 433 677 1148 245
Cuts at: 5805 1606
Size: 2120
Fragments arranged by size:
2120 1253 1148 677 433 245 225 119
99
NaeI GCC'GGC
Cuts at: 2364 3454 2364
Size: 1090 5229
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5767
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6319
NgoMIV G'CCGG_C
Cuts at: 2362 3452 2362
Size: 1090 5229
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6319
NotI GC'GGCC_GC
Cuts at: 3766 3766
Size: 6319
NspI r_CATG'y
Cuts at: 1132 1229 2234 2452 3677 5222 1132
Size: 97 1005 218 1225 1545 2229
Fragments arranged by size:
2229 1545 1225 1005 218 97
PasI CC'CwG_GG
Cuts at: 1348 2502 3311 3328 3396 1348
Size: 1154 809 17 68 4271
Fragments arranged by size:
4271 1154 809 68 17
PciI A'CATG_T
Cuts at: 5218 5218
Size: 6319
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2269 3057 3574 1108
Size: 1161 788 517 3853
Fragments arranged by size:
3853 1161 788 517
PfoI T'CCnGG_A
Cuts at: 2112 3339 2112
Size: 1227 5092
PmeI GTTT'AAAC
Cuts at: 3623 3623
Size: 6319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4388 4420 5901 5933 4388
Size: 32 1481 32 4774
Fragments arranged by size:
4774 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2640 2915 3250 3545 2640
Size: 275 335 295 5414
Fragments arranged by size:
5414 335 295 275
PshAI GACnn'nnGTC
Cuts at: 1613 3275 1613
Size: 1662 4657
PsiI TTA'TAA
Cuts at: 3889 3889
Size: 6319
PspOMI G'GGCC_C
Cuts at: 2612 3323 2612
Size: 711 5608
PspXI vC'TCGA_Gb
Cuts at: 2584 2584
Size: 6319
PstI C_TGCA'G
Cuts at: 830 2201 2481 2601 2858 2994 3182 3503 3671
Size: 1371 280 120 257 136 188 321 168
Cuts at: 3671 830
Size: 3478
Fragments arranged by size:
3478 1371 321 280 257 188 168 136
120
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6319
PvuII CAG'CTG
Cuts at: 2958 3183 3302 2958
Size: 225 119 5975
Fragments arranged by size:
5975 225 119
SalI G'TCGA_C
Cuts at: 1936 3661 1936
Size: 1725 4594
SanDI GG'GwC_CC
Cuts at: 2915 2915
Size: 6319
SapI GCTCTTCn'nnn_
Cuts at: 2166 2166
Size: 6319
SbfI CC_TGCA'GG
Cuts at: 3671 3671
Size: 6319
ScaI AGT'ACT
Cuts at: 4567 6279 4567
Size: 1712 4607
SfcI C'TryA_G
Cuts at: 826 1045 2197 2477 2597 2854 2990 3178 3499
Size: 219 1152 280 120 257 136 188 321
Cuts at: 3499 3667 4802 5483 5674 826
Size: 168 1135 681 191 1471
Fragments arranged by size:
1471 1152 1135 681 321 280 257 219
191 188 168 136 120
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6319
SmaI CCC'GGG
Cuts at: 1872 3535 3646 1872
Size: 1663 111 4545
Fragments arranged by size:
4545 1663 111
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2542 2584 4423 5324 5586 820
Size: 197 926 599 42 1839 901 262 1553
Fragments arranged by size:
1839 1553 926 901 599 262 197 42
SnaBI TAC'GTA
Cuts at: 493 4110 493
Size: 3617 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6319
SphI G_CATG'C
Cuts at: 1132 2234 3677 1132
Size: 1102 1443 3774
Fragments arranged by size:
3774 1443 1102
SspI AAT'ATT
Cuts at: 5 52 4243 5
Size: 47 4191 2081
Fragments arranged by size:
4191 2081 47
StuI AGG'CCT
Cuts at: 1517 3243 1517
Size: 1726 4593
StyI C'CwwG_G
Cuts at: 513 1065 3758 513
Size: 552 2693 3074
Fragments arranged by size:
3074 2693 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2866 2870 2979 3562 4346 4363 4516
Size: 1255 1286 4 109 583 784 17 153
Cuts at: 4516 4701 5146 325
Size: 185 445 1498
Fragments arranged by size:
1498 1286 1255 784 583 445 185 153
109 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2481 2844 2952
Size: 274 80 33 51 1443 504 363 108
Cuts at: 2952 4565 6277 96
Size: 1613 1712 138
Fragments arranged by size:
1712 1613 1443 504 363 274 138 108
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2632 2664 6279 6311 2632
Size: 32 3615 32 2640
Fragments arranged by size:
3615 2640 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2072 2663 2680 1316
Size: 756 591 17 4955
Fragments arranged by size:
4955 756 591 17
XbaI T'CTAG_A
Cuts at: 3655 3655
Size: 6319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2908 2908
Size: 6319
XhoI C'TCGA_G
Cuts at: 1943 2584 1943
Size: 641 5678
XmaI C'CCGG_G
Cuts at: 1870 3533 3644 1870
Size: 1663 111 4545
Fragments arranged by size:
4545 1663 111
XmnI GAAnn'nnTTC
Cuts at: 4448 4448
Size: 6319
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4127 276
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FspAI
KasI NarI NruI NsiI PacI PmlI PsrI RsrII
SacI SacII SexAI SfiI SfoI SrfI SwaI