Restriction map for
pFN21AB5927
(Circular) (Six-base) MAPSORT of: pFN21AB5927.seq Check: 5856 from: 1 to: 6406
pFN21AB5927 6406 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2317 2317
Size: 6406
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4216 278
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2303 3414 3749 1937
Size: 366 1111 335 4594
Fragments arranged by size:
4594 1111 366 335
Acc65I G'GTAC_C
Cuts at: 3727 3727
Size: 6406
AclI AA'CG_TT
Cuts at: 1563 4533 4906 1563
Size: 2970 373 3063
Fragments arranged by size:
3063 2970 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2693 4469 5853 6121 1632
Size: 1061 1776 1384 268 1917
Fragments arranged by size:
1917 1776 1384 1061 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6209
AflIII A'CryG_T
Cuts at: 1203 5305 6282 1203
Size: 4102 977 1327
Fragments arranged by size:
4102 1327 977
AgeI A'CCGG_T
Cuts at: 5237 5237
Size: 6406
AhdI GACnn_n'nnGTC
Cuts at: 3695 5135 3695
Size: 1440 4966
AleI CACnn'nnGTG
Cuts at: 1166 2189 1166
Size: 1023 5383
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2141 1184
Size: 957 5449
AlwNI CAG_nnn'CTG
Cuts at: 2373 4149 5721 2373
Size: 1776 1572 3058
Fragments arranged by size:
3058 1776 1572
ApaI G_GGCC'C
Cuts at: 2749 2749
Size: 6406
ApaLI G'TGCA_C
Cuts at: 4463 5619 4463
Size: 1156 5250
ApoI r'AATT_y
Cuts at: 1493 2540 2558 3275 3715 3941 4093 6202 1493
Size: 1047 18 717 440 226 152 2109 1697
Fragments arranged by size:
2109 1697 1047 717 440 226 152 18
AseI AT'TA_AT
Cuts at: 160 1032 4960 160
Size: 872 3928 1606
Fragments arranged by size:
3928 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
AvaI C'yCGr_G
Cuts at: 1870 1943 3731 1870
Size: 73 1788 4545
Fragments arranged by size:
4545 1788 73
AvrII C'CTAG_G
Cuts at: 2908 2908
Size: 6406
BamHI G'GATC_C
Cuts at: 3319 3589 3736 4103 3319
Size: 270 147 367 5622
Fragments arranged by size:
5622 367 270 147
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3727 5182 618
Size: 325 217 627 1940 1455 1842
Fragments arranged by size:
1940 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2018 2749 2936 3632 3725 1391
Size: 627 731 187 696 93 4072
Fragments arranged by size:
4072 731 696 627 187 93
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2539 2790 3434 928
Size: 971 640 251 644 3900
Fragments arranged by size:
3900 971 644 640 251
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4597 4631 1903
Size: 34 2660 34 3678
Fragments arranged by size:
3678 2660 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4298 5508 4298
Size: 1210 5196
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6406
BfrBI ATG'CAT
Cuts at: 3509 3509
Size: 6406
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2919 5017 136
Size: 107 122 71 631 605 1247 2098 1525
Fragments arranged by size:
2098 1525 1247 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6401 6401
Size: 6406
BlpI GC'TnA_GC
Cuts at: 3626 3823 3626
Size: 197 6209
Bme1580I G_kGCm'C
Cuts at: 1790 2040 2749 4467 5623 1790
Size: 250 709 1718 1156 2573
Fragments arranged by size:
2573 1718 1156 709 250
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6406
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2585 3277 4137
Size: 416 53 83 399 24 1168 692 860
Cuts at: 4137 5095 442
Size: 958 1753
Fragments arranged by size:
1753 1168 958 860 692 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2400 3374 3523 5066 1239
Size: 1161 974 149 1543 2579
Fragments arranged by size:
2579 1543 1161 974 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4531 5396 5694 4531
Size: 865 298 5243
Fragments arranged by size:
5243 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2320 3074 5069 882
Size: 617 821 754 1995 2219
Fragments arranged by size:
2219 1995 821 754 617
BsaAI yAC'GTr
Cuts at: 493 1204 3448 4197 493
Size: 711 2244 749 2702
Fragments arranged by size:
2702 2244 749 711
BsaBI GATnn'nnATC
Cuts at: 2601 6400 2601
Size: 3799 2607
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4213 4595 5230 275
Size: 53 83 186 936 2680 382 635 1451
Fragments arranged by size:
2680 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4839 5237 5511 5658 6069 4839
Size: 398 274 147 411 5176
Fragments arranged by size:
5176 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6181
Fragments arranged by size:
6181 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2868 3031 1186
Size: 1682 163 4561
Fragments arranged by size:
4561 1682 163
BseYI C'CCAG_C
Cuts at: 2663 2740 2749 3085 3593 5609 6105 2663
Size: 77 9 336 508 2016 496 2964
Fragments arranged by size:
2964 2016 508 496 336 77 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2167 2264 2418 1163
Size: 573 431 97 154 5151
Fragments arranged by size:
5151 573 431 154 97
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2066 2070 2173 3856 4617 5645
Size: 275 186 65 4 103 1683 761 1028
Cuts at: 5645 1540
Size: 2301
Fragments arranged by size:
2301 1683 1028 761 275 186 103 65
4
BsiHKAI G_wGCw'C
Cuts at: 4467 4552 5623 4467
Size: 85 1071 5250
Fragments arranged by size:
5250 1071 85
BsmI GAATG_Cn'
Cuts at: 2976 3917 4010 2976
Size: 941 93 5372
Fragments arranged by size:
5372 941 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2040 2749 2936 3632 3725 4467
Size: 399 228 22 709 187 696 93 742
Cuts at: 4467 4552 5623 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 709 696 399 228 187
93 85 22
BspEI T'CCGG_A
Cuts at: 6069 6069
Size: 6406
BspHI T'CATG_A
Cuts at: 3342 3667 4295 6025 3342
Size: 325 628 1730 3723
Fragments arranged by size:
3723 1730 628 325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2133 2172 2199 2317 3288 3761 844
Size: 1289 39 27 118 971 473 3489
Fragments arranged by size:
3489 1289 971 473 118 39 27
BsrBI CCG'CTC
Cuts at: 1394 1823 4293 1394
Size: 429 2470 3507
Fragments arranged by size:
3507 2470 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2697 2850 2955 4901 5075 65
Size: 1183 1449 153 105 1946 174 1396
Fragments arranged by size:
1946 1449 1396 1183 174 153 105
BsrFI r'CCGG_y
Cuts at: 1700 1810 2065 2069 2096 5050 5237 1700
Size: 110 255 4 27 2954 187 2869
Fragments arranged by size:
2954 2869 255 187 110 27 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6406
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6406
BssSI C'ACGA_G
Cuts at: 4466 5478 4466
Size: 1012 5394
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2882 794
Size: 2088 4318
BstBI TT'CG_AA
Cuts at: 5210 5210
Size: 6406
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6406
BstXI CCAn_nnnn'nTGG
Cuts at: 2194 2378 3093 2194
Size: 184 715 5507
Fragments arranged by size:
5507 715 184
BstYI r'GATC_y
Cuts at: 1972 3319 3589 3736 4103 4488 4505 5957 6043
Size: 1347 270 147 367 385 17 1452 86
Cuts at: 6043 6055 6401 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1347 385 367 346 270 147
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3488 3488
Size: 6406
BtgI C'CryG_G
Cuts at: 513 1065 2004 2187 2253 3004 513
Size: 552 939 183 66 751 3915
Fragments arranged by size:
3915 939 751 552 183 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3628 487
Size: 683 56 2402 3265
Fragments arranged by size:
3265 2402 683 56
BtsI GCAGTG_nn'
Cuts at: 2371 3627 3922 4716 4736 2371
Size: 1256 295 794 20 4041
Fragments arranged by size:
4041 1256 794 295 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6406
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3121 3156 3275 3310 529
Size: 35 2557 35 119 35 3625
Fragments arranged by size:
3625 2557 119 35 35 35
DraI TTT'AAA
Cuts at: 3710 3710
Size: 6406
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5413 809
Size: 507 4097 1802
Fragments arranged by size:
4097 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2063 2067 2226
Size: 54 1425 50 275 18 233 4 159
Cuts at: 2226 2250 2256 2949 3051 3280 3385 3598 3679
Size: 24 6 693 102 229 105 213 81
Cuts at: 3679 3853 4742 6371 8
Size: 174 889 1629 43
Fragments arranged by size:
1629 1425 889 693 275 233 229 213
174 159 105 102 81 54 50 43
24 18 6 4
EagI C'GGCC_G
Cuts at: 1537 1812 2063 2067 3853 1537
Size: 275 251 4 1786 4090
Fragments arranged by size:
4090 1786 275 251 4
EarI CTCTTCn'nnn_
Cuts at: 1353 2108 2838 3141 4336 1353
Size: 755 730 303 1195 3423
Fragments arranged by size:
3423 1195 755 730 303
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4993 5367 5513 387
Size: 1064 138 3404 374 146 1280
Fragments arranged by size:
3404 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2400 2693 3374 3523 4469 5066 5853
Size: 393 768 293 681 149 946 597 787
Cuts at: 5853 6121 1239
Size: 268 1524
Fragments arranged by size:
1524 946 787 768 681 597 393 293
268 149
EcoNI CCTnn'n_nnAGG
Cuts at: 2105 2105
Size: 6406
EcoO109I rG'GnC_Cy
Cuts at: 2429 2708 2745 2746 3067 3349 3358 3484 3689
Size: 279 37 1 321 282 9 126 205
Cuts at: 3689 2429
Size: 5146
Fragments arranged by size:
5146 321 282 279 205 126 37 9
1
EcoRI G'AATT_C
Cuts at: 3715 4093 3715
Size: 378 6028
EcoRV GAT'ATC
Cuts at: 2599 2599
Size: 6406
FseI GG_CCGG'CC
Cuts at: 2069 2069
Size: 6406
FspI TGC'GCA
Cuts at: 4912 4912
Size: 6406
HaeII r_GCGC'y
Cuts at: 1678 5553 1678
Size: 3875 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2432 2464 3681 3713 5047
Size: 32 135 32 860 32 1217 32 1334
Cuts at: 5047 5079 5121 5153 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1334 1217 860 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2304 3750 3996 669
Size: 988 281 366 1446 246 3079
Fragments arranged by size:
3079 1446 988 366 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6406
HpaI GTT'AAC
Cuts at: 3996 3996
Size: 6406
KpnI G_GTAC'C
Cuts at: 3731 3731
Size: 6406
MfeI C'AATT_G
Cuts at: 4005 4005
Size: 6406
MluI A'CGCG_T
Cuts at: 6282 6282
Size: 6406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3297 5520 5704 1305
Size: 1992 2223 184 2007
Fragments arranged by size:
2223 2007 1992 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2951 3053 3387 10
Size: 54 1425 343 1119 102 334 3029
Fragments arranged by size:
3029 1425 1119 343 334 102 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2024 2189 2192 4364 4723 4882 518
Size: 648 858 165 3 2172 359 159 2042
Fragments arranged by size:
2172 2042 858 648 359 165 159 3
MspA1I CmG'CkG
Cuts at: 1606 2062 2255 2345 3006 3089 3597 3822 4499
Size: 456 193 90 661 83 508 225 677
Cuts at: 4499 5647 5892 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 661 508 456 245 225
193 90 83
NaeI GCC'GGC
Cuts at: 2067 2071 2067
Size: 4 6402
NcoI C'CATG_G
Cuts at: 513 1065 2004 2187 513
Size: 552 939 183 4732
Fragments arranged by size:
4732 939 552 183
NdeI CA'TA_TG
Cuts at: 387 2821 387
Size: 2434 3972
NgoMIV G'CCGG_C
Cuts at: 2065 2069 2065
Size: 4 6402
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6406
NotI GC'GGCC_GC
Cuts at: 3853 3853
Size: 6406
NsiI A_TGCA'T
Cuts at: 3511 3511
Size: 6406
NspI r_CATG'y
Cuts at: 1132 1229 3764 5309 1132
Size: 97 2535 1545 2229
Fragments arranged by size:
2535 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6406
PciI A'CATG_T
Cuts at: 5305 5305
Size: 6406
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6406
PmeI GTTT'AAAC
Cuts at: 3710 3710
Size: 6406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4475 4507 5988 6020 4475
Size: 32 1481 32 4861
Fragments arranged by size:
4861 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2429 2708 3067 3349 3689 2429
Size: 279 359 282 340 5146
Fragments arranged by size:
5146 359 340 282 279
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6406
PsiI TTA'TAA
Cuts at: 3976 3976
Size: 6406
PspOMI G'GGCC_C
Cuts at: 2745 2745
Size: 6406
PstI C_TGCA'G
Cuts at: 830 3758 830
Size: 2928 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6406
PvuII CAG'CTG
Cuts at: 2345 3089 2345
Size: 744 5662
SacII CC_GC'GG
Cuts at: 2256 3007 2256
Size: 751 5655
SalI G'TCGA_C
Cuts at: 1936 2302 3748 1936
Size: 366 1446 4594
Fragments arranged by size:
4594 1446 366
SanDI GG'GwC_CC
Cuts at: 3689 3689
Size: 6406
SbfI CC_TGCA'GG
Cuts at: 3758 3758
Size: 6406
ScaI AGT'ACT
Cuts at: 4654 6366 4654
Size: 1712 4694
SfcI C'TryA_G
Cuts at: 826 1045 3415 3754 4889 5570 5761 826
Size: 219 2370 339 1135 681 191 1471
Fragments arranged by size:
2370 1471 1135 681 339 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6406
SmaI CCC'GGG
Cuts at: 1872 3733 1872
Size: 1861 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4510 5411 5673 820
Size: 197 926 2567 901 262 1553
Fragments arranged by size:
2567 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4197 493
Size: 3704 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6406
SphI G_CATG'C
Cuts at: 1132 3764 1132
Size: 2632 3774
SspI AAT'ATT
Cuts at: 5 52 4330 5
Size: 47 4278 2081
Fragments arranged by size:
4278 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6406
StyI C'CwwG_G
Cuts at: 513 1065 2004 2187 2908 3845 513
Size: 552 939 183 721 937 3074
Fragments arranged by size:
3074 939 937 721 552 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4433 4450 4603 4788 5233 325
Size: 1255 2853 17 153 185 445 1498
Fragments arranged by size:
2853 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4652 6364 96
Size: 274 80 33 51 1443 2675 1712 138
Fragments arranged by size:
2675 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2700 2732 3179 3211 3226 3258 6366 6398 2700
Size: 32 447 32 15 32 3108 32 2708
Fragments arranged by size:
3108 2708 447 32 32 32 32 15
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6406
XbaI T'CTAG_A
Cuts at: 3742 3742
Size: 6406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2236 3037 2236
Size: 801 5605
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6406
XmaI C'CCGG_G
Cuts at: 1870 3731 1870
Size: 1861 4545
XmnI GAAnn'nnTTC
Cuts at: 4535 4535
Size: 6406
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4214 276
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BplI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoICRI FalI FspAI KasI
NarI NruI PacI PfoI PmlI PspXI PsrI RsrII
SacI SapI SexAI SfiI SfoI SrfI SwaI