Restriction map for
pFN21AB5926
(Circular) (Six-base) MAPSORT of: pFN21AB5926.seq Check: 1989 from: 1 to: 6313
pFN21AB5926 6313 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3376 3376
Size: 6313
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4123 278
Size: 53 83 186 936 2587 2468
Fragments arranged by size:
2587 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3656 1937
Size: 1719 4594
Acc65I G'GTAC_C
Cuts at: 3634 3634
Size: 6313
AclI AA'CG_TT
Cuts at: 1563 4440 4813 1563
Size: 2877 373 3063
Fragments arranged by size:
3063 2877 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2583 2909 3605 4376 5760 6028 1632
Size: 951 326 696 771 1384 268 1917
Fragments arranged by size:
1917 1384 951 771 696 326 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6116
AflIII A'CryG_T
Cuts at: 1203 5212 6189 1203
Size: 4009 977 1327
Fragments arranged by size:
4009 1327 977
AgeI A'CCGG_T
Cuts at: 5144 5144
Size: 6313
AhdI GACnn_n'nnGTC
Cuts at: 5042 5042
Size: 6313
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6313
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3062 1184
Size: 1878 4435
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2285 2317 2567 2599 2285
Size: 32 250 32 5999
Fragments arranged by size:
5999 250 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2101 2561 2884 2887 3245 3532 4056 5628 2101
Size: 460 323 3 358 287 524 1572 2786
Fragments arranged by size:
2786 1572 524 460 358 323 287 3
ApaI G_GGCC'C
Cuts at: 3611 3611
Size: 6313
ApaLI G'TGCA_C
Cuts at: 4370 5526 4370
Size: 1156 5157
ApoI r'AATT_y
Cuts at: 1493 3622 3848 4000 6109 1493
Size: 2129 226 152 2109 1697
Fragments arranged by size:
2129 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4867 160
Size: 872 3835 1606
Fragments arranged by size:
3835 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
AvaI C'yCGr_G
Cuts at: 1870 1943 2195 3638 1870
Size: 73 252 1443 4545
Fragments arranged by size:
4545 1443 252 73
BamHI G'GATC_C
Cuts at: 3117 3643 4010 3117
Size: 526 367 5420
Fragments arranged by size:
5420 526 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2053 3634 5089 618
Size: 325 217 627 266 1581 1455 1842
Fragments arranged by size:
1842 1581 1455 627 325 266 217
BanII G_rGCy'C
Cuts at: 1391 2028 2175 2346 3611 3632 1391
Size: 637 147 171 1265 21 4072
Fragments arranged by size:
4072 1265 637 171 147 21
BbeI G_GCGC'C
Cuts at: 2057 2057
Size: 6313
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2897 928
Size: 971 998 4344
Fragments arranged by size:
4344 998 971
BbvCI CC'TCA_GC
Cuts at: 2037 3006 3074 3136 2037
Size: 969 68 62 5214
Fragments arranged by size:
5214 969 68 62
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4504 4538 1903
Size: 34 2567 34 3678
Fragments arranged by size:
3678 2567 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4205 5415 4205
Size: 1210 5103
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6313
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2526 4924 136
Size: 107 122 71 631 605 854 2398 1525
Fragments arranged by size:
2398 1525 854 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3324 6308 3324
Size: 2984 3329
BlpI GC'TnA_GC
Cuts at: 3730 3730
Size: 6313
Bme1580I G_kGCm'C
Cuts at: 1790 2410 2992 3611 4374 5530 1790
Size: 620 582 619 763 1156 2573
Fragments arranged by size:
2573 1156 763 620 619 582
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6313
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2191 2927 4044
Size: 416 53 83 399 24 774 736 1117
Cuts at: 4044 5002 442
Size: 958 1753
Fragments arranged by size:
1753 1117 958 774 736 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 2267 2538 3209 3311 4973 1239
Size: 851 177 271 671 102 1662 2579
Fragments arranged by size:
2579 1662 851 671 271 177 102
Bpu10I CC'TnA_GC
Cuts at: 2037 3006 3074 3136 2037
Size: 969 68 62 5214
Fragments arranged by size:
5214 969 68 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4438 5303 5601 4438
Size: 865 298 5150
Fragments arranged by size:
5150 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2695 3029 3515 4976 882
Size: 617 1196 334 486 1461 2219
Fragments arranged by size:
2219 1461 1196 617 486 334
BsaAI yAC'GTr
Cuts at: 493 1204 4104 493
Size: 711 2900 2702
Fragments arranged by size:
2900 2702 711
BsaBI GATnn'nnATC
Cuts at: 6307 6307
Size: 6313
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2054 4120 4502 5137
Size: 53 83 186 936 521 2066 382 635
Cuts at: 5137 275
Size: 1451
Fragments arranged by size:
2066 1451 936 635 521 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2014 4746 5144 5418 5565 5976 2014
Size: 2732 398 274 147 411 2351
Fragments arranged by size:
2732 2351 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2567 2597 2891 2921 1178
Size: 30 165 30 1164 30 294 30 4570
Fragments arranged by size:
4570 1164 294 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2228 2336 2585 2915 1186
Size: 1042 108 249 330 4584
Fragments arranged by size:
4584 1042 330 249 108
BseYI C'CCAG_C
Cuts at: 2152 2166 2367 2515 2586 2966 2991 3316 3532
Size: 14 201 148 71 380 25 325 216
Cuts at: 3532 5516 6012 2152
Size: 1984 496 2453
Fragments arranged by size:
2453 1984 496 380 325 216 201 148
71 25 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2246 3022 1163
Size: 573 510 776 4454
Fragments arranged by size:
4454 776 573 510
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2223 2242 3763 4524 5552 1540
Size: 275 186 222 19 1521 761 1028 2301
Fragments arranged by size:
2301 1521 1028 761 275 222 186 19
BsiHKAI G_wGCw'C
Cuts at: 2145 3225 4374 4459 5530 2145
Size: 1080 1149 85 1071 2928
Fragments arranged by size:
2928 1149 1080 1071 85
BsmI GAATG_Cn'
Cuts at: 3824 3917 3824
Size: 93 6220
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2145 2175 2346 2410 2992 3225
Size: 399 238 117 30 171 64 582 233
Cuts at: 3225 3611 3632 4374 4459 5530 1391
Size: 386 21 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 582 399 386 238 233
171 117 85 64 30 21
BspEI T'CCGG_A
Cuts at: 5976 5976
Size: 6313
BspHI T'CATG_A
Cuts at: 2689 4202 5932 2689
Size: 1513 1730 3070
Fragments arranged by size:
3070 1730 1513
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3376 3668 844
Size: 2532 292 3489
Fragments arranged by size:
3489 2532 292
BsrBI CCG'CTC
Cuts at: 1394 1823 3382 4200 1394
Size: 429 1559 818 3507
Fragments arranged by size:
3507 1559 818 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3085 4808 4982 65
Size: 1183 1837 1723 174 1396
Fragments arranged by size:
1837 1723 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2050 2218 4957 5144 1700
Size: 110 240 168 2739 187 2869
Fragments arranged by size:
2869 2739 240 187 168 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6313
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6313
BssSI C'ACGA_G
Cuts at: 4373 5385 4373
Size: 1012 5301
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2101 2884 794
Size: 1307 783 4223
Fragments arranged by size:
4223 1307 783
BstBI TT'CG_AA
Cuts at: 2801 5117 2801
Size: 2316 3997
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6313
BstXI CCAn_nnnn'nTGG
Cuts at: 2594 2974 3463 2594
Size: 380 489 5444
Fragments arranged by size:
5444 489 380
BstYI r'GATC_y
Cuts at: 1972 3117 3324 3643 4010 4395 4412 5864 5950
Size: 1145 207 319 367 385 17 1452 86
Cuts at: 5950 5962 6308 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1145 385 367 346 319 207
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2115 2637 3572 2115
Size: 522 935 4856
Fragments arranged by size:
4856 935 522
BtgI C'CryG_G
Cuts at: 513 1065 2004 2161 2451 3462 513
Size: 552 939 157 290 1011 3364
Fragments arranged by size:
3364 1011 939 552 290 157
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2310 487
Size: 683 56 1084 4490
Fragments arranged by size:
4490 1084 683 56
BtsI GCAGTG_nn'
Cuts at: 3829 4623 4643 3829
Size: 794 20 5499
Fragments arranged by size:
5499 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6313
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6278
DraI TTT'AAA
Cuts at: 3617 3617
Size: 6313
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2768 5320 809
Size: 507 1452 2552 1802
Fragments arranged by size:
2552 1802 1452 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2239 2313 2321
Size: 54 1425 50 275 18 409 74 8
Cuts at: 2321 3760 4649 6278 8
Size: 1439 889 1629 43
Fragments arranged by size:
1629 1439 1425 889 409 275 74 54
50 43 18 8
EagI C'GGCC_G
Cuts at: 1537 1812 2239 3760 1537
Size: 275 427 1521 4090
Fragments arranged by size:
4090 1521 427 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2562 2616 2639 2670 4243 1353
Size: 1209 54 23 31 1573 3423
Fragments arranged by size:
3423 1573 1209 54 31 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4900 5274 5420 387
Size: 1064 138 3311 374 146 1280
Fragments arranged by size:
3311 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2267 2538 2583 2909 3209 3311
Size: 393 458 177 271 45 326 300 102
Cuts at: 3311 3605 4376 4973 5760 6028 1239
Size: 294 771 597 787 268 1524
Fragments arranged by size:
1524 787 771 597 458 393 326 300
294 271 268 177 102 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2093 3571 2093
Size: 1478 4835
EcoO109I rG'GnC_Cy
Cuts at: 2074 2111 2200 2231 2374 2445 2650 3229 3242
Size: 37 89 31 143 71 205 579 13
Cuts at: 3242 3608 2074
Size: 366 4779
Fragments arranged by size:
4779 579 366 205 143 89 71 37
31 13
EcoRI G'AATT_C
Cuts at: 3622 4000 3622
Size: 378 5935
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3610 3610
Size: 6313
FspI TGC'GCA
Cuts at: 4819 4819
Size: 6313
HaeII r_GCGC'y
Cuts at: 1678 2057 2102 2153 3207 5460 1678
Size: 379 45 51 1054 2253 2531
Fragments arranged by size:
2531 2253 1054 379 51 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3105 3137 3487 3519 4954
Size: 32 135 32 1533 32 350 32 1435
Cuts at: 4954 4986 5028 5060 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1533 1435 350 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3657 3903 669
Size: 988 281 1719 246 3079
Fragments arranged by size:
3079 1719 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6313
HpaI GTT'AAC
Cuts at: 3903 3903
Size: 6313
KasI G'GCGC_C
Cuts at: 2053 2053
Size: 6313
KpnI G_GTAC'C
Cuts at: 3638 3638
Size: 6313
MfeI C'AATT_G
Cuts at: 3912 3912
Size: 6313
MluI A'CGCG_T
Cuts at: 6189 6189
Size: 6313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5427 5611 1305
Size: 4122 184 2007
Fragments arranged by size:
4122 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4491
Fragments arranged by size:
4491 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3461 4271 4630 4789 518
Size: 648 2295 810 359 159 2042
Fragments arranged by size:
2295 2042 810 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2068 2163 2884 2995 3729 4406 5554 5799
Size: 462 95 721 111 734 677 1148 245
Cuts at: 5799 1606
Size: 2120
Fragments arranged by size:
2120 1148 734 721 677 462 245 111
95
NaeI GCC'GGC
Cuts at: 2052 2052
Size: 6313
NarI GG'CG_CC
Cuts at: 2054 2054
Size: 6313
NcoI C'CATG_G
Cuts at: 513 1065 2004 2451 3462 513
Size: 552 939 447 1011 3364
Fragments arranged by size:
3364 1011 939 552 447
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6313
NgoMIV G'CCGG_C
Cuts at: 2050 2050
Size: 6313
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6313
NotI GC'GGCC_GC
Cuts at: 3760 3760
Size: 6313
NspI r_CATG'y
Cuts at: 1132 1229 3671 5216 1132
Size: 97 2442 1545 2229
Fragments arranged by size:
2442 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2030 2058 2410 1348
Size: 682 28 352 5251
Fragments arranged by size:
5251 682 352 28
PciI A'CATG_T
Cuts at: 5212 5212
Size: 6313
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3532 1108
Size: 2424 3889
PfoI T'CCnGG_A
Cuts at: 2856 2856
Size: 6313
PmeI GTTT'AAAC
Cuts at: 3617 3617
Size: 6313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2567 2599 4382 4414 5895 5927 2567
Size: 32 1783 32 1481 32 2953
Fragments arranged by size:
2953 1783 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2374 2445 2650 3242 2374
Size: 71 205 592 5445
Fragments arranged by size:
5445 592 205 71
PshAI GACnn'nnGTC
Cuts at: 1613 2184 2241 3021 1613
Size: 571 57 780 4905
Fragments arranged by size:
4905 780 571 57
PsiI TTA'TAA
Cuts at: 3883 3883
Size: 6313
PspOMI G'GGCC_C
Cuts at: 3607 3607
Size: 6313
PspXI vC'TCGA_Gb
Cuts at: 2195 2195
Size: 6313
PstI C_TGCA'G
Cuts at: 830 2097 2106 2361 2880 3000 3203 3665 830
Size: 1267 9 255 519 120 203 462 3478
Fragments arranged by size:
3478 1267 519 462 255 203 120 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6313
PvuII CAG'CTG
Cuts at: 2068 2884 2995 2068
Size: 816 111 5386
Fragments arranged by size:
5386 816 111
SacII CC_GC'GG
Cuts at: 2164 2164
Size: 6313
SalI G'TCGA_C
Cuts at: 1936 3655 1936
Size: 1719 4594
SanDI GG'GwC_CC
Cuts at: 2650 2650
Size: 6313
SbfI CC_TGCA'GG
Cuts at: 3665 3665
Size: 6313
ScaI AGT'ACT
Cuts at: 4561 6273 4561
Size: 1712 4601
SfcI C'TryA_G
Cuts at: 826 1045 2093 2102 2357 2876 2942 2996 3199
Size: 219 1048 9 255 519 66 54 203
Cuts at: 3199 3661 4796 5477 5668 826
Size: 462 1135 681 191 1471
Fragments arranged by size:
1471 1135 1048 681 519 462 255 219
203 191 66 54 9
SfoI GGC'GCC
Cuts at: 2055 2055
Size: 6313
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6313
SmaI CCC'GGG
Cuts at: 1872 3640 1872
Size: 1768 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2195 4417 5318 5580 820
Size: 197 926 252 2222 901 262 1553
Fragments arranged by size:
2222 1553 926 901 262 252 197
SnaBI TAC'GTA
Cuts at: 493 4104 493
Size: 3611 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6313
SphI G_CATG'C
Cuts at: 1132 3671 1132
Size: 2539 3774
SspI AAT'ATT
Cuts at: 5 52 4237 5
Size: 47 4185 2081
Fragments arranged by size:
4185 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6313
StyI C'CwwG_G
Cuts at: 513 1065 2004 2451 2895 3462 3752 513
Size: 552 939 447 444 567 290 3074
Fragments arranged by size:
3074 939 567 552 447 444 290
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3011 4340 4357 4510 4695 5140 325
Size: 1255 1431 1329 17 153 185 445 1498
Fragments arranged by size:
1498 1431 1329 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4559 6271 96
Size: 274 80 33 51 1443 2582 1712 138
Fragments arranged by size:
2582 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2969 3001 6273 6305 2969
Size: 32 3272 32 2977
Fragments arranged by size:
3272 2977 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6313
XbaI T'CTAG_A
Cuts at: 3649 3649
Size: 6313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3460 3460
Size: 6313
XhoI C'TCGA_G
Cuts at: 1943 2195 1943
Size: 252 6061
XmaI C'CCGG_G
Cuts at: 1870 3638 1870
Size: 1768 4545
XmnI GAAnn'nnTTC
Cuts at: 4442 4442
Size: 6313
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4121 276
Size: 53 83 186 936 2587 2468
Fragments arranged by size:
2587 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI
PacI PmlI PsrI RsrII SacI SapI SexAI SfiI
SrfI SwaI