Restriction map for
pFN21AB5925
(Circular) (Six-base) MAPSORT of: pFN21AB5925.seq Check: 7466 from: 1 to: 6217
pFN21AB5925 6217 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2866 2866
Size: 6217
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4027 278
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3560 1937
Size: 1623 4594
Acc65I G'GTAC_C
Cuts at: 3538 3538
Size: 6217
AclI AA'CG_TT
Cuts at: 1563 4344 4717 1563
Size: 2781 373 3063
Fragments arranged by size:
3063 2781 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2075 2635 4280 5664 5932 1632
Size: 443 560 1645 1384 268 1917
Fragments arranged by size:
1917 1645 1384 560 443 268
AfeI AGC'GCT
Cuts at: 2954 2954
Size: 6217
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6020
AflIII A'CryG_T
Cuts at: 1203 5116 6093 1203
Size: 3913 977 1327
Fragments arranged by size:
3913 1327 977
AgeI A'CCGG_T
Cuts at: 5048 5048
Size: 6217
AhdI GACnn_n'nnGTC
Cuts at: 4946 4946
Size: 6217
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6217
AlwNI CAG_nnn'CTG
Cuts at: 2725 2974 3960 5532 2725
Size: 249 986 1572 3410
Fragments arranged by size:
3410 1572 986 249
ApaI G_GGCC'C
Cuts at: 2325 2688 2812 3489 2325
Size: 363 124 677 5053
Fragments arranged by size:
5053 677 363 124
ApaLI G'TGCA_C
Cuts at: 4274 5430 4274
Size: 1156 5061
ApoI r'AATT_y
Cuts at: 1493 3526 3752 3904 6013 1493
Size: 2033 226 152 2109 1697
Fragments arranged by size:
2109 2033 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4771 160
Size: 872 3739 1606
Fragments arranged by size:
3739 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
AvaI C'yCGr_G
Cuts at: 1870 1943 2325 2805 3246 3542 1870
Size: 73 382 480 441 296 4545
Fragments arranged by size:
4545 480 441 382 296 73
BamHI G'GATC_C
Cuts at: 3547 3914 3547
Size: 367 5850
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3277 3538 4993 618
Size: 325 217 627 1490 261 1455 1842
Fragments arranged by size:
1842 1490 1455 627 325 261 217
BanII G_rGCy'C
Cuts at: 1391 2325 2688 2812 2997 3159 3405 3489 3536
Size: 934 363 124 185 162 246 84 47
Cuts at: 3536 1391
Size: 4072
Fragments arranged by size:
4072 934 363 246 185 162 124 84
47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5246
BbvCI CC'TCA_GC
Cuts at: 2340 3076 2340
Size: 736 5481
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2953 2987 4408 4442 1903
Size: 34 1016 34 1421 34 3678
Fragments arranged by size:
3678 1421 1016 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3353 4109 5319 3353
Size: 756 1210 4251
Fragments arranged by size:
4251 1210 756
BclI T'GATC_A
Cuts at: 1548 2739 1548
Size: 1191 5026
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2252 4828 136
Size: 107 122 71 631 605 580 2576 1525
Fragments arranged by size:
2576 1525 631 605 580 122 107 71
BglII A'GATC_T
Cuts at: 2352 3204 3354 6212 2352
Size: 852 150 2858 2357
Fragments arranged by size:
2858 2357 852 150
BlpI GC'TnA_GC
Cuts at: 3634 3634
Size: 6217
Bme1580I G_kGCm'C
Cuts at: 1790 2284 2325 2688 2812 3489 4278 5434 1790
Size: 494 41 363 124 677 789 1156 2573
Fragments arranged by size:
2573 1156 789 677 494 363 124 41
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6217
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2015 2934 3948
Size: 416 53 83 399 24 598 919 1014
Cuts at: 3948 4906 442
Size: 958 1753
Fragments arranged by size:
1753 1014 958 919 598 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6217
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 2428 2680 3328 2383
Size: 45 252 648 5272
Fragments arranged by size:
5272 648 252 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2164 2589 2696 2792 2879 2924 2987 3079
Size: 925 425 107 96 87 45 63 92
Cuts at: 3079 3158 3323 3480 4877 1239
Size: 79 165 157 1397 2579
Fragments arranged by size:
2579 1397 925 425 165 157 107 96
92 87 79 63 45
Bpu10I CC'TnA_GC
Cuts at: 2340 2474 3076 2340
Size: 134 602 5481
Fragments arranged by size:
5481 602 134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4342 5207 5505 4342
Size: 865 298 5054
Fragments arranged by size:
5054 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4880 882
Size: 617 3381 2219
Fragments arranged by size:
3381 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4008 493
Size: 711 2804 2702
Fragments arranged by size:
2804 2702 711
BsaBI GATnn'nnATC
Cuts at: 6211 6211
Size: 6217
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4024 4406 5041 275
Size: 53 83 186 936 2491 382 635 1451
Fragments arranged by size:
2491 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4650 5048 5322 5469 5880 4650
Size: 398 274 147 411 4987
Fragments arranged by size:
4987 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2765 2795 1178
Size: 30 165 30 1362 30 4600
Fragments arranged by size:
4600 1362 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2444 2447 2616 1186
Size: 1258 3 169 4787
Fragments arranged by size:
4787 1258 169 3
BseYI C'CCAG_C
Cuts at: 2168 2394 2590 2612 2921 3423 3502 5420 5916
Size: 226 196 22 309 502 79 1918 496
Cuts at: 5916 2168
Size: 2469
Fragments arranged by size:
2469 1918 502 496 309 226 196 79
22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2442 2513 1163
Size: 573 706 71 4867
Fragments arranged by size:
4867 706 573 71
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3667 4428 5456 1540
Size: 275 186 1666 761 1028 2301
Fragments arranged by size:
2301 1666 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3159 3405 4278 4363 5434 3159
Size: 246 873 85 1071 3942
Fragments arranged by size:
3942 1071 873 246 85
BsmI GAATG_Cn'
Cuts at: 3728 3821 3728
Size: 93 6124
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2284 2325 2688 2812 2997 3159 3405
Size: 399 494 41 363 124 185 162 246
Cuts at: 3405 3489 3536 4278 4363 5434 1391
Size: 84 47 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 494 399 363 246 185
162 124 85 84 47 41
BspEI T'CCGG_A
Cuts at: 5880 5880
Size: 6217
BspHI T'CATG_A
Cuts at: 4106 5836 4106
Size: 1730 4487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2280 2742 2866 3572 844
Size: 1436 462 124 706 3489
Fragments arranged by size:
3489 1436 706 462 124
BsrBI CCG'CTC
Cuts at: 1394 1823 3063 4104 1394
Size: 429 1240 1041 3507
Fragments arranged by size:
3507 1240 1041 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4712 4886 65
Size: 1183 3464 174 1396
Fragments arranged by size:
3464 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2838 4861 5048 1700
Size: 110 1028 2023 187 2869
Fragments arranged by size:
2869 2023 1028 187 110
BsrGI T'GTAC_A
Cuts at: 96 3268 96
Size: 3172 3045
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6217
BssSI C'ACGA_G
Cuts at: 4277 5289 4277
Size: 1012 5205
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3433 794
Size: 2639 3578
BstBI TT'CG_AA
Cuts at: 5021 5021
Size: 6217
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6217
BstXI CCAn_nnnn'nTGG
Cuts at: 2023 2620 2902 2023
Size: 597 282 5338
Fragments arranged by size:
5338 597 282
BstYI r'GATC_y
Cuts at: 1972 2352 3204 3354 3547 3914 4299 4316 5768
Size: 380 852 150 193 367 385 17 1452
Cuts at: 5768 5854 5866 6212 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 852 385 380 367 346 193
150 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2403 2928 2403
Size: 525 5692
BtgI C'CryG_G
Cuts at: 513 1065 2583 2830 2989 513
Size: 552 1518 247 159 3741
Fragments arranged by size:
3741 1518 552 247 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5478
Fragments arranged by size:
5478 683 56
BtsI GCAGTG_nn'
Cuts at: 2496 3733 4527 4547 2496
Size: 1237 794 20 4166
Fragments arranged by size:
4166 1237 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6182
DraI TTT'AAA
Cuts at: 3521 3521
Size: 6217
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5224 809
Size: 507 3908 1802
Fragments arranged by size:
3908 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2253 2546 2798
Size: 54 1425 50 275 18 423 293 252
Cuts at: 2798 3478 3664 4553 6182 8
Size: 680 186 889 1629 43
Fragments arranged by size:
1629 1425 889 680 423 293 275 252
186 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3664 1537
Size: 275 1852 4090
Fragments arranged by size:
4090 1852 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2436 2442 2849 2891 4147 1353
Size: 1083 6 407 42 1256 3423
Fragments arranged by size:
3423 1256 1083 407 42 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2294 3023 4804 5178 5324 387
Size: 1064 138 705 729 1781 374 146 1280
Fragments arranged by size:
1781 1280 1064 729 705 374 146 138
EcoICRI GAG'CTC
Cuts at: 3157 3403 3157
Size: 246 5971
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2164 2589 2635 2696 2792 2879
Size: 393 443 89 425 46 61 96 87
Cuts at: 2879 2924 2987 3079 3158 3323 3480 4280 4877
Size: 45 63 92 79 165 157 800 597
Cuts at: 4877 5664 5932 1239
Size: 787 268 1524
Fragments arranged by size:
1524 800 787 597 443 425 393 268
165 157 96 92 89 87 79 63
61 46 45
EcoNI CCTnn'n_nnAGG
Cuts at: 3462 3462
Size: 6217
EcoO109I rG'GnC_Cy
Cuts at: 2322 2594 2671 2685 2809 3019 3451 3485 3486
Size: 272 77 14 124 210 432 34 1
Cuts at: 3486 2322
Size: 5053
Fragments arranged by size:
5053 432 272 210 124 77 34 14
1
EcoRI G'AATT_C
Cuts at: 3526 3904 3526
Size: 378 5839
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2971 2971
Size: 6217
FspI TGC'GCA
Cuts at: 4723 4723
Size: 6217
HaeII r_GCGC'y
Cuts at: 1678 2956 5364 1678
Size: 1278 2408 2531
Fragments arranged by size:
2531 2408 1278
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4858 4890 4932 4964 1373
Size: 32 135 32 3286 32 42 32 2626
Fragments arranged by size:
3286 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2113 3561 3807 669
Size: 988 281 175 1448 246 3079
Fragments arranged by size:
3079 1448 988 281 246 175
HindIII A'AGCT_T
Cuts at: 748 3125 748
Size: 2377 3840
HpaI GTT'AAC
Cuts at: 3807 3807
Size: 6217
KpnI G_GTAC'C
Cuts at: 3542 3542
Size: 6217
MfeI C'AATT_G
Cuts at: 3816 3816
Size: 6217
MluI A'CGCG_T
Cuts at: 6093 6093
Size: 6217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5331 5515 1305
Size: 4026 184 2007
Fragments arranged by size:
4026 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3480 10
Size: 54 1425 343 1648 2747
Fragments arranged by size:
2747 1648 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2021 4175 4534 4693 518
Size: 648 855 2154 359 159 2042
Fragments arranged by size:
2154 2042 855 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2085 2857 2971 2991 3109 3335 3430 3506
Size: 479 772 114 20 118 226 95 76
Cuts at: 3506 3633 4310 5458 5703 1606
Size: 127 677 1148 245 2120
Fragments arranged by size:
2120 1148 772 677 479 245 226 127
118 114 95 76 20
NaeI GCC'GGC
Cuts at: 2840 2840
Size: 6217
NcoI C'CATG_G
Cuts at: 513 1065 2830 513
Size: 552 1765 3900
Fragments arranged by size:
3900 1765 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6217
NgoMIV G'CCGG_C
Cuts at: 2838 2838
Size: 6217
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6217
NotI GC'GGCC_GC
Cuts at: 3664 3664
Size: 6217
NspI r_CATG'y
Cuts at: 1132 1229 2153 2335 2755 3514 3575 5120 1132
Size: 97 924 182 420 759 61 1545 2229
Fragments arranged by size:
2229 1545 924 759 420 182 97 61
PasI CC'CwG_GG
Cuts at: 1348 2096 1348
Size: 748 5469
PciI A'CATG_T
Cuts at: 5116 5116
Size: 6217
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2974 1108
Size: 1866 4351
PfoI T'CCnGG_A
Cuts at: 2673 2982 3457 2673
Size: 309 475 5433
Fragments arranged by size:
5433 475 309
PmeI GTTT'AAAC
Cuts at: 3521 3521
Size: 6217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4286 4318 5799 5831 4286
Size: 32 1481 32 4672
Fragments arranged by size:
4672 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2594 2671 3019 2594
Size: 77 348 5792
Fragments arranged by size:
5792 348 77
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6217
PsiI TTA'TAA
Cuts at: 3787 3787
Size: 6217
PspOMI G'GGCC_C
Cuts at: 2321 2684 2808 3485 2321
Size: 363 124 677 5053
Fragments arranged by size:
5053 677 363 124
PstI C_TGCA'G
Cuts at: 830 2457 2562 2577 2713 3569 830
Size: 1627 105 15 136 856 3478
Fragments arranged by size:
3478 1627 856 136 105 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6217
PvuII CAG'CTG
Cuts at: 2085 2857 2971 3335 3506 2085
Size: 772 114 364 171 4796
Fragments arranged by size:
4796 772 364 171 114
SacI G_AGCT'C
Cuts at: 3159 3405 3159
Size: 246 5971
SacII CC_GC'GG
Cuts at: 2992 2992
Size: 6217
SalI G'TCGA_C
Cuts at: 1936 3559 1936
Size: 1623 4594
SanDI GG'GwC_CC
Cuts at: 2594 2594
Size: 6217
SapI GCTCTTCn'nnn_
Cuts at: 2442 2849 2442
Size: 407 5810
SbfI CC_TGCA'GG
Cuts at: 2713 3569 2713
Size: 856 5361
ScaI AGT'ACT
Cuts at: 4465 6177 4465
Size: 1712 4505
SfcI C'TryA_G
Cuts at: 826 1045 2453 2558 2573 2709 2848 3008 3282
Size: 219 1408 105 15 136 139 160 274
Cuts at: 3282 3350 3565 4700 5381 5572 826
Size: 68 215 1135 681 191 1471
Fragments arranged by size:
1471 1408 1135 681 274 219 215 191
160 139 136 105 68 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6217
SmaI CCC'GGG
Cuts at: 1872 2327 2807 3544 1872
Size: 455 480 737 4545
Fragments arranged by size:
4545 737 480 455
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4321 5222 5484 820
Size: 197 926 2378 901 262 1553
Fragments arranged by size:
2378 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4008 493
Size: 3515 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6217
SphI G_CATG'C
Cuts at: 1132 2153 3514 3575 1132
Size: 1021 1361 61 3774
Fragments arranged by size:
3774 1361 1021 61
SspI AAT'ATT
Cuts at: 5 52 4141 5
Size: 47 4089 2081
Fragments arranged by size:
4089 2081 47
StuI AGG'CCT
Cuts at: 1517 2201 3468 1517
Size: 684 1267 4266
Fragments arranged by size:
4266 1267 684
StyI C'CwwG_G
Cuts at: 513 1065 2208 2830 3469 3656 513
Size: 552 1143 622 639 187 3074
Fragments arranged by size:
3074 1143 639 622 552 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2780 2882 3516 4244 4261 4414 4599
Size: 1255 1200 102 634 728 17 153 185
Cuts at: 4599 5044 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1200 728 634 445 185 153
102 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3268 4463 6175
Size: 274 80 33 51 1443 1291 1195 1712
Cuts at: 6175 96
Size: 138
Fragments arranged by size:
1712 1443 1291 1195 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2528 2560 6177 6209 2528
Size: 32 3617 32 2536
Fragments arranged by size:
3617 2536 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6217
XbaI T'CTAG_A
Cuts at: 3553 3553
Size: 6217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2103 3455 2103
Size: 1352 4865
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6217
XmaI C'CCGG_G
Cuts at: 1870 2325 2805 3542 1870
Size: 455 480 737 4545
Fragments arranged by size:
4545 737 480 455
XmnI GAAnn'nnTTC
Cuts at: 2129 4346 2129
Size: 2217 4000
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4025 276
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI PsrI RsrII SexAI SfiI
SfoI SrfI SwaI