Restriction map for
pFN21AB5924
(Circular) (Six-base) MAPSORT of: pFN21AB5924.seq Check: 4982 from: 1 to: 6265
pFN21AB5924 6265 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4075 278
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2721 3003 3608 1937
Size: 784 282 605 4594
Fragments arranged by size:
4594 784 605 282
Acc65I G'GTAC_C
Cuts at: 3586 3586
Size: 6265
AclI AA'CG_TT
Cuts at: 1563 4392 4765 1563
Size: 2829 373 3063
Fragments arranged by size:
3063 2829 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2835 3222 3450 4328 5712 5980 1632
Size: 1203 387 228 878 1384 268 1917
Fragments arranged by size:
1917 1384 1203 878 387 268 228
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6068
AflIII A'CryG_T
Cuts at: 1203 2186 3247 5164 6141 1203
Size: 983 1061 1917 977 1327
Fragments arranged by size:
1917 1327 1061 983 977
AgeI A'CCGG_T
Cuts at: 5096 5096
Size: 6265
AhdI GACnn_n'nnGTC
Cuts at: 2539 4994 2539
Size: 2455 3810
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3291 1184
Size: 2107 4158
AlwNI CAG_nnn'CTG
Cuts at: 2278 3428 4008 5580 2278
Size: 1150 580 1572 2963
Fragments arranged by size:
2963 1572 1150 580
ApaLI G'TGCA_C
Cuts at: 4322 5478 4322
Size: 1156 5109
ApoI r'AATT_y
Cuts at: 1493 2105 2468 3043 3149 3281 3574 3800 3952
Size: 612 363 575 106 132 293 226 152
Cuts at: 3952 6061 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 612 575 363 293 226 152
132 106
AseI AT'TA_AT
Cuts at: 160 1032 4819 160
Size: 872 3787 1606
Fragments arranged by size:
3787 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
AvaI C'yCGr_G
Cuts at: 1870 1943 3126 3590 1870
Size: 73 1183 464 4545
Fragments arranged by size:
4545 1183 464 73
BamHI G'GATC_C
Cuts at: 3595 3962 3595
Size: 367 5898
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3586 5041 618
Size: 325 217 627 1799 1455 1842
Fragments arranged by size:
1842 1799 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2562 3133 3584 1391
Size: 1171 571 451 4072
Fragments arranged by size:
4072 1171 571 451
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2823 928
Size: 971 924 4370
Fragments arranged by size:
4370 971 924
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4456 4490 1903
Size: 34 2519 34 3678
Fragments arranged by size:
3678 2519 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4157 5367 4157
Size: 1210 5055
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6265
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2690 2927 4876
Size: 107 122 71 631 605 1018 237 1949
Cuts at: 4876 136
Size: 1525
Fragments arranged by size:
1949 1525 1018 631 605 237 122 107
71
BglII A'GATC_T
Cuts at: 2586 6260 2586
Size: 3674 2591
BlpI GC'TnA_GC
Cuts at: 2405 3682 2405
Size: 1277 4988
Bme1580I G_kGCm'C
Cuts at: 1790 4326 5482 1790
Size: 2536 1156 2573
Fragments arranged by size:
2573 2536 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6265
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2333 2946 3996
Size: 416 53 83 399 24 916 613 1050
Cuts at: 3996 4954 442
Size: 958 1753
Fragments arranged by size:
1753 1050 958 916 613 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6265
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2769 3568 2769
Size: 799 5466
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2209 2546 3081 3402 4925 1239
Size: 970 337 535 321 1523 2579
Fragments arranged by size:
2579 1523 970 535 337 321
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4390 5255 5553 4390
Size: 865 298 5102
Fragments arranged by size:
5102 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3539 4928 882
Size: 617 2040 1389 2219
Fragments arranged by size:
2219 2040 1389 617
BsaAI yAC'GTr
Cuts at: 493 1204 4056 493
Size: 711 2852 2702
Fragments arranged by size:
2852 2702 711
BsaBI GATnn'nnATC
Cuts at: 2754 6259 2754
Size: 3505 2760
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4072 4454 5089 275
Size: 53 83 186 936 2539 382 635 1451
Fragments arranged by size:
2539 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2463 4698 5096 5370 5517 5928 2463
Size: 2235 398 274 147 411 2800
Fragments arranged by size:
2800 2235 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6040
Fragments arranged by size:
6040 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2646 3405 3473 1186
Size: 841 619 759 68 3978
Fragments arranged by size:
3978 841 759 619 68
BseYI C'CCAG_C
Cuts at: 5468 5964 5468
Size: 496 5769
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5692
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3715 4476 5504 1540
Size: 275 186 1714 761 1028 2301
Fragments arranged by size:
2301 1714 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2562 4326 4411 5482 2562
Size: 1764 85 1071 3345
Fragments arranged by size:
3345 1764 1071 85
BsmI GAATG_Cn'
Cuts at: 2217 3349 3776 3869 2217
Size: 1132 427 93 4613
Fragments arranged by size:
4613 1132 427 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2562 3133 3584 4326 4411 5482 1391
Size: 399 772 571 451 742 85 1071 2174
Fragments arranged by size:
2174 1071 772 742 571 451 399 85
BspEI T'CCGG_A
Cuts at: 5928 5928
Size: 6265
BspHI T'CATG_A
Cuts at: 3240 4154 5884 3240
Size: 914 1730 3621
Fragments arranged by size:
3621 1730 914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3620 844
Size: 2776 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2062 4152 1394
Size: 429 239 2090 3507
Fragments arranged by size:
3507 2090 429 239
BsrDI GCAATG_nn'
Cuts at: 65 1248 2848 2869 4760 4934 65
Size: 1183 1600 21 1891 174 1396
Fragments arranged by size:
1891 1600 1396 1183 174 21
BsrFI r'CCGG_y
Cuts at: 1700 1810 2039 4909 5096 1700
Size: 110 229 2870 187 2869
Fragments arranged by size:
2870 2869 229 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6265
BssHII G'CGCG_C
Cuts at: 1925 2017 1925
Size: 92 6173
BssSI C'ACGA_G
Cuts at: 4325 5337 4325
Size: 1012 5253
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2278 794
Size: 1484 4781
BstBI TT'CG_AA
Cuts at: 5069 5069
Size: 6265
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6265
BstYI r'GATC_y
Cuts at: 1972 2586 3595 3962 4347 4364 5816 5902 5914
Size: 614 1009 367 385 17 1452 86 12
Cuts at: 5914 6260 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1009 614 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3004 3004
Size: 6265
Bsu36I CC'TnA_GG
Cuts at: 2069 2667 2069
Size: 598 5667
BtgI C'CryG_G
Cuts at: 513 1065 2004 2997 513
Size: 552 939 993 3781
Fragments arranged by size:
3781 993 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5526
Fragments arranged by size:
5526 683 56
BtsI GCAGTG_nn'
Cuts at: 3781 4575 4595 3781
Size: 794 20 5451
Fragments arranged by size:
5451 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6265
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6230
DraI TTT'AAA
Cuts at: 2158 3569 2158
Size: 1411 4854
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3260 5272 809
Size: 507 1944 2012 1802
Fragments arranged by size:
2012 1944 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2634 3363 3712
Size: 54 1425 50 275 18 804 729 349
Cuts at: 3712 4601 6230 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 804 729 349 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3712 1537
Size: 275 1900 4090
Fragments arranged by size:
4090 1900 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2513 2711 2823 3050 3382 3537 4195 1353
Size: 1160 198 112 227 332 155 658 3423
Fragments arranged by size:
3423 1160 658 332 227 198 155 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4852 5226 5372 387
Size: 1064 138 3263 374 146 1280
Fragments arranged by size:
3263 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2560 2560
Size: 6265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2209 2546 2835 3081 3222 3402 3450
Size: 393 577 337 289 246 141 180 48
Cuts at: 3450 4328 4925 5712 5980 1239
Size: 878 597 787 268 1524
Fragments arranged by size:
1524 878 787 597 577 393 337 289
268 246 180 141 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2168 2168
Size: 6265
EcoO109I rG'GnC_Cy
Cuts at: 2065 2692 3370 2065
Size: 627 678 4960
Fragments arranged by size:
4960 678 627
EcoRI G'AATT_C
Cuts at: 3281 3574 3952 3281
Size: 293 378 5594
Fragments arranged by size:
5594 378 293
FspI TGC'GCA
Cuts at: 4771 4771
Size: 6265
HaeII r_GCGC'y
Cuts at: 1678 5412 1678
Size: 3734 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2312 2344 2525 2557 2615
Size: 32 135 32 740 32 181 32 58
Cuts at: 2615 2643 2647 2675 4906 4938 4980 5012 1373
Size: 28 4 28 2231 32 42 32 2626
Fragments arranged by size:
2626 2231 740 181 135 58 42 32
32 32 32 32 32 28 28 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2851 2904 3253 3609 3855 669
Size: 988 281 913 53 349 356 246 3079
Fragments arranged by size:
3079 988 913 356 349 281 246 53
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6265
HpaI GTT'AAC
Cuts at: 2904 3855 2904
Size: 951 5314
KpnI G_GTAC'C
Cuts at: 3590 3590
Size: 6265
MfeI C'AATT_G
Cuts at: 2546 3864 2546
Size: 1318 4947
MluI A'CGCG_T
Cuts at: 6141 6141
Size: 6265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2708 2834 5379 5563 1305
Size: 1403 126 2545 184 2007
Fragments arranged by size:
2545 2007 1403 184 126
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4443
Fragments arranged by size:
4443 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2359 2841 4223 4582 4741 518
Size: 648 1193 482 1382 359 159 2042
Fragments arranged by size:
2042 1382 1193 648 482 359 159
MspA1I CmG'CkG
Cuts at: 1606 2575 2777 2966 3681 4358 5506 5751 1606
Size: 969 202 189 715 677 1148 245 2120
Fragments arranged by size:
2120 1148 969 715 677 245 202 189
NaeI GCC'GGC
Cuts at: 2041 2041
Size: 6265
NcoI C'CATG_G
Cuts at: 513 1065 2004 2997 513
Size: 552 939 993 3781
Fragments arranged by size:
3781 993 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6265
NgoMIV G'CCGG_C
Cuts at: 2039 2039
Size: 6265
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6265
NotI GC'GGCC_GC
Cuts at: 3712 3712
Size: 6265
NspI r_CATG'y
Cuts at: 1132 1229 2190 3251 3623 5168 1132
Size: 97 961 1061 372 1545 2229
Fragments arranged by size:
2229 1545 1061 961 372 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6265
PciI A'CATG_T
Cuts at: 2186 3247 5164 2186
Size: 1061 1917 3287
Fragments arranged by size:
3287 1917 1061
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6265
PmeI GTTT'AAAC
Cuts at: 3569 3569
Size: 6265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4334 4366 5847 5879 4334
Size: 32 1481 32 4720
Fragments arranged by size:
4720 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2065 2065
Size: 6265
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6265
PsiI TTA'TAA
Cuts at: 2168 3835 2168
Size: 1667 4598
PstI C_TGCA'G
Cuts at: 830 3352 3617 830
Size: 2522 265 3478
Fragments arranged by size:
3478 2522 265
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6265
PvuII CAG'CTG
Cuts at: 2777 2966 2777
Size: 189 6076
SacI G_AGCT'C
Cuts at: 2562 2562
Size: 6265
SalI G'TCGA_C
Cuts at: 1936 3607 1936
Size: 1671 4594
SapI GCTCTTCn'nnn_
Cuts at: 2513 2823 2513
Size: 310 5955
SbfI CC_TGCA'GG
Cuts at: 3617 3617
Size: 6265
ScaI AGT'ACT
Cuts at: 2211 3530 4513 6225 2211
Size: 1319 983 1712 2251
Fragments arranged by size:
2251 1712 1319 983
SfcI C'TryA_G
Cuts at: 826 1045 3079 3348 3613 4748 5429 5620 826
Size: 219 2034 269 265 1135 681 191 1471
Fragments arranged by size:
2034 1471 1135 681 269 265 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6265
SmaI CCC'GGG
Cuts at: 1872 3592 1872
Size: 1720 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4369 5270 5532 820
Size: 197 926 2426 901 262 1553
Fragments arranged by size:
2426 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4056 493
Size: 3563 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6265
SphI G_CATG'C
Cuts at: 1132 3623 1132
Size: 2491 3774
SspI AAT'ATT
Cuts at: 5 52 4189 5
Size: 47 4137 2081
Fragments arranged by size:
4137 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6265
StyI C'CwwG_G
Cuts at: 513 1065 2004 2997 3256 3704 513
Size: 552 939 993 259 448 3074
Fragments arranged by size:
3074 993 939 552 448 259
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2633 4292 4309 4462 4647 5092 325
Size: 1255 1053 1659 17 153 185 445 1498
Fragments arranged by size:
1659 1498 1255 1053 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2209 3528 4511
Size: 274 80 33 51 1443 232 1319 983
Cuts at: 4511 6223 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1319 983 274 232 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6225 6257 6225
Size: 32 6233
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6265
XbaI T'CTAG_A
Cuts at: 2938 3601 2938
Size: 663 5602
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3373 3373
Size: 6265
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6265
XmaI C'CCGG_G
Cuts at: 1870 3590 1870
Size: 1720 4545
XmnI GAAnn'nnTTC
Cuts at: 4394 4394
Size: 6265
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4073 276
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI DraIII EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI