Restriction map for
pFN21AB5922
(Circular) (Six-base) MAPSORT of: pFN21AB5922.seq Check: 820 from: 1 to: 6304
pFN21AB5922 6304 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4114 278
Size: 53 83 186 936 2578 2468
Fragments arranged by size:
2578 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3647 1937
Size: 1710 4594
Acc65I G'GTAC_C
Cuts at: 3597 3625 3597
Size: 28 6276
AclI AA'CG_TT
Cuts at: 1563 4431 4804 1563
Size: 2868 373 3063
Fragments arranged by size:
3063 2868 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2301 3502 4367 5751 6019 1632
Size: 669 1201 865 1384 268 1917
Fragments arranged by size:
1917 1384 1201 865 669 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6107
AflIII A'CryG_T
Cuts at: 1203 5203 6180 1203
Size: 4000 977 1327
Fragments arranged by size:
4000 1327 977
AgeI A'CCGG_T
Cuts at: 5135 5135
Size: 6304
AhdI GACnn_n'nnGTC
Cuts at: 2389 3490 5033 2389
Size: 1101 1543 3660
Fragments arranged by size:
3660 1543 1101
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6304
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2918 1184
Size: 1734 4570
AlwNI CAG_nnn'CTG
Cuts at: 3305 4047 5619 3305
Size: 742 1572 3990
Fragments arranged by size:
3990 1572 742
ApaLI G'TGCA_C
Cuts at: 3574 4361 5517 3574
Size: 787 1156 4361
Fragments arranged by size:
4361 1156 787
ApoI r'AATT_y
Cuts at: 1493 3250 3613 3839 3991 6100 1493
Size: 1757 363 226 152 2109 1697
Fragments arranged by size:
2109 1757 1697 363 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4858 160
Size: 872 3826 1606
Fragments arranged by size:
3826 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
AvaI C'yCGr_G
Cuts at: 1870 1943 2828 3629 1870
Size: 73 885 801 4545
Fragments arranged by size:
4545 885 801 73
BamHI G'GATC_C
Cuts at: 3634 4001 3634
Size: 367 5937
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2188 2413 3597 3625 5080
Size: 325 217 627 401 225 1184 28 1455
Cuts at: 5080 618
Size: 1842
Fragments arranged by size:
1842 1455 1184 627 401 325 225 217
28
BanII G_rGCy'C
Cuts at: 1391 2137 3020 3623 1391
Size: 746 883 603 4072
Fragments arranged by size:
4072 883 746 603
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2340 928
Size: 971 441 4892
Fragments arranged by size:
4892 971 441
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2045 2079 4495 4529 1903
Size: 34 108 34 2416 34 3678
Fragments arranged by size:
3678 2416 108 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4196 5406 4196
Size: 1210 5094
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6304
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4915 136
Size: 107 122 71 631 605 3243 1525
Fragments arranged by size:
3243 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3038 6299 3038
Size: 3261 3043
BlpI GC'TnA_GC
Cuts at: 3721 3721
Size: 6304
Bme1580I G_kGCm'C
Cuts at: 1790 2025 3578 4365 5521 1790
Size: 235 1553 787 1156 2573
Fragments arranged by size:
2573 1553 1156 787 235
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6304
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3596 4035 4993
Size: 416 53 83 399 24 2179 439 958
Cuts at: 4993 442
Size: 1753
Fragments arranged by size:
2179 1753 958 439 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3108 4964 1239
Size: 1869 1856 2579
Fragments arranged by size:
2579 1869 1856
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3135 4429 5294 5592 3135
Size: 1294 865 298 3847
Fragments arranged by size:
3847 1294 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2572 2977 4967 882
Size: 617 1073 405 1990 2219
Fragments arranged by size:
2219 1990 1073 617 405
BsaAI yAC'GTr
Cuts at: 493 1204 4095 493
Size: 711 2891 2702
Fragments arranged by size:
2891 2702 711
BsaBI GATnn'nnATC
Cuts at: 2976 6298 2976
Size: 3322 2982
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4111 4493 5128 275
Size: 53 83 186 936 2578 382 635 1451
Fragments arranged by size:
2578 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4737 5135 5409 5556 5967 4737
Size: 398 274 147 411 5074
Fragments arranged by size:
5074 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2498 2528 2710 2740 1178
Size: 30 165 30 1095 30 182 30 4742
Fragments arranged by size:
4742 1095 182 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2251 2254 2417 1186
Size: 1065 3 163 5073
Fragments arranged by size:
5073 1065 163 3
BseYI C'CCAG_C
Cuts at: 2046 2553 2660 2994 5507 6003 2046
Size: 507 107 334 2513 496 2347
Fragments arranged by size:
2513 2347 507 496 334 107
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5731
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2530 2614 3754 4515 5543 1540
Size: 275 186 529 84 1140 761 1028 2301
Fragments arranged by size:
2301 1140 1028 761 529 275 186 84
BsiHKAI G_wGCw'C
Cuts at: 3020 3578 4365 4450 5521 3020
Size: 558 787 85 1071 3803
Fragments arranged by size:
3803 1071 787 558 85
BsmI GAATG_Cn'
Cuts at: 3092 3815 3908 3092
Size: 723 93 5488
Fragments arranged by size:
5488 723 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2137 3020 3578 3623 4365 4450
Size: 399 235 112 883 558 45 742 85
Cuts at: 4450 5521 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 883 742 558 399 235 112
85 45
BspEI T'CCGG_A
Cuts at: 5967 5967
Size: 6304
BspHI T'CATG_A
Cuts at: 4193 5923 4193
Size: 1730 4574
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3659 844
Size: 2815 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4191 1394
Size: 429 2368 3507
Fragments arranged by size:
3507 2368 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4799 4973 65
Size: 1183 3551 174 1396
Fragments arranged by size:
3551 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2529 2609 4948 5135 1700
Size: 110 719 80 2339 187 2869
Fragments arranged by size:
2869 2339 719 187 110 80
BsrGI T'GTAC_A
Cuts at: 96 2520 3396 96
Size: 2424 876 3004
Fragments arranged by size:
3004 2424 876
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6304
BssSI C'ACGA_G
Cuts at: 4364 5376 4364
Size: 1012 5292
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3305 794
Size: 2511 3793
BstBI TT'CG_AA
Cuts at: 5108 5108
Size: 6304
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6304
BstXI CCAn_nnnn'nTGG
Cuts at: 2129 2129
Size: 6304
BstYI r'GATC_y
Cuts at: 1972 3038 3423 3634 4001 4386 4403 5855 5941
Size: 1066 385 211 367 385 17 1452 86
Cuts at: 5941 5953 6299 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1066 385 385 367 346 211
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2270 2418 2874 3237 513
Size: 552 939 266 148 456 363 3580
Fragments arranged by size:
3580 939 552 456 363 266 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5565
Fragments arranged by size:
5565 683 56
BtsI GCAGTG_nn'
Cuts at: 2115 2627 3820 4614 4634 2115
Size: 512 1193 794 20 3785
Fragments arranged by size:
3785 1193 794 512 20
ClaI AT'CG_AT
Cuts at: 1554 2127 1554
Size: 573 5731
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6269
DraI TTT'AAA
Cuts at: 3608 3608
Size: 6304
DraIII CAC_nnn'GTG
Cuts at: 3125 3125
Size: 6304
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5311 809
Size: 507 3995 1802
Fragments arranged by size:
3995 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2586 2611 3751
Size: 54 1425 50 275 18 756 25 1140
Cuts at: 3751 4640 6269 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1140 889 756 275 54 50
43 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 2611 3751 1537
Size: 275 799 1140 4090
Fragments arranged by size:
4090 1140 799 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2226 2277 2295 2403 2947 3240 3258 4234
Size: 873 51 18 108 544 293 18 976
Cuts at: 4234 1353
Size: 3423
Fragments arranged by size:
3423 976 873 544 293 108 51 18
18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4891 5265 5411 387
Size: 1064 138 3302 374 146 1280
Fragments arranged by size:
3302 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3018 3018
Size: 6304
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2301 3108 3502 4367 4964 5751 6019
Size: 393 669 807 394 865 597 787 268
Cuts at: 6019 1239
Size: 1524
Fragments arranged by size:
1524 865 807 787 669 597 394 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2038 2050 3155 2038
Size: 12 1105 5187
Fragments arranged by size:
5187 1105 12
EcoRI G'AATT_C
Cuts at: 3250 3613 3991 3250
Size: 363 378 5563
Fragments arranged by size:
5563 378 363
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3326 3326
Size: 6304
FspI TGC'GCA
Cuts at: 4810 4810
Size: 6304
HaeII r_GCGC'y
Cuts at: 1678 5451 1678
Size: 3773 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2375 2407 2789 2821 3159
Size: 32 135 32 803 32 382 32 338
Cuts at: 3159 3191 3415 3447 3476 3508 4945 4977 5019
Size: 32 224 32 29 32 1437 32 42
Cuts at: 5019 5051 1373
Size: 32 2626
Fragments arranged by size:
2626 1437 803 382 338 224 135 42
32 32 32 32 32 32 32 32
32 29
HincII GTy'rAC
Cuts at: 669 1657 1938 3648 3894 669
Size: 988 281 1710 246 3079
Fragments arranged by size:
3079 1710 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6304
HpaI GTT'AAC
Cuts at: 3894 3894
Size: 6304
KpnI G_GTAC'C
Cuts at: 3601 3629 3601
Size: 28 6276
MfeI C'AATT_G
Cuts at: 3903 3903
Size: 6304
MluI A'CGCG_T
Cuts at: 6180 6180
Size: 6304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2535 3045 5418 5602 1305
Size: 1230 510 2373 184 2007
Fragments arranged by size:
2373 2007 1230 510 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4482
Fragments arranged by size:
4482 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2127 4262 4621 4780 518
Size: 648 961 2135 359 159 2042
Fragments arranged by size:
2135 2042 961 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2272 2816 3239 3720 4397 5545 5790 1606
Size: 666 544 423 481 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 666 544 481 423 245
NaeI GCC'GGC
Cuts at: 2611 2611
Size: 6304
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4813
Fragments arranged by size:
4813 939 552
NdeI CA'TA_TG
Cuts at: 387 3379 387
Size: 2992 3312
NgoMIV G'CCGG_C
Cuts at: 2609 2609
Size: 6304
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6304
NotI GC'GGCC_GC
Cuts at: 3751 3751
Size: 6304
NspI r_CATG'y
Cuts at: 1132 1229 3085 3590 3662 5207 1132
Size: 97 1856 505 72 1545 2229
Fragments arranged by size:
2229 1856 1545 505 97 72
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6304
PciI A'CATG_T
Cuts at: 5203 5203
Size: 6304
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2933 1108
Size: 1825 4479
PmeI GTTT'AAAC
Cuts at: 3608 3608
Size: 6304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4373 4405 5886 5918 4373
Size: 32 1481 32 4759
Fragments arranged by size:
4759 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2038 2050 2038
Size: 12 6292
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6304
PsiI TTA'TAA
Cuts at: 3874 3874
Size: 6304
PstI C_TGCA'G
Cuts at: 830 2475 3595 3656 830
Size: 1645 1120 61 3478
Fragments arranged by size:
3478 1645 1120 61
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6304
SacI G_AGCT'C
Cuts at: 3020 3020
Size: 6304
SacII CC_GC'GG
Cuts at: 2273 3240 2273
Size: 967 5337
SalI G'TCGA_C
Cuts at: 1936 3646 1936
Size: 1710 4594
SanDI GG'GwC_CC
Cuts at: 2050 2050
Size: 6304
SbfI CC_TGCA'GG
Cuts at: 3656 3656
Size: 6304
ScaI AGT'ACT
Cuts at: 4552 6264 4552
Size: 1712 4592
SfcI C'TryA_G
Cuts at: 826 1045 2471 3591 3652 4787 5468 5659 826
Size: 219 1426 1120 61 1135 681 191 1471
Fragments arranged by size:
1471 1426 1135 1120 681 219 191 61
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6304
SmaI CCC'GGG
Cuts at: 1872 3631 1872
Size: 1759 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3150 4408 5309 5571 820
Size: 197 926 1207 1258 901 262 1553
Fragments arranged by size:
1553 1258 1207 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4095 493
Size: 3602 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6304
SphI G_CATG'C
Cuts at: 1132 3590 3662 1132
Size: 2458 72 3774
Fragments arranged by size:
3774 2458 72
SspI AAT'ATT
Cuts at: 5 52 4228 5
Size: 47 4176 2081
Fragments arranged by size:
4176 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6304
StyI C'CwwG_G
Cuts at: 513 1065 2004 3743 513
Size: 552 939 1739 3074
Fragments arranged by size:
3074 1739 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2596 4331 4348 4501 4686 5131 325
Size: 1255 1016 1735 17 153 185 445 1498
Fragments arranged by size:
1735 1498 1255 1016 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2520 3396 4550
Size: 274 80 33 51 1443 543 876 1154
Cuts at: 4550 6262 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1154 876 543 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2710 2742 6264 6296 2710
Size: 32 3522 32 2718
Fragments arranged by size:
3522 2718 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6304
XbaI T'CTAG_A
Cuts at: 3640 3640
Size: 6304
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2934 2934
Size: 6304
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6304
XmaI C'CCGG_G
Cuts at: 1870 3629 1870
Size: 1759 4545
XmnI GAAnn'nnTTC
Cuts at: 4433 4433
Size: 6304
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4112 276
Size: 53 83 186 936 2578 2468
Fragments arranged by size:
2578 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacI SacII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SapI SexAI SfiI SfoI SrfI SwaI