Restriction map for
pFN21AB5921
(Circular) (Six-base) MAPSORT of: pFN21AB5921.seq Check: 7715 from: 1 to: 6220
pFN21AB5921 6220 bp
With 182 enzymes: SgfI *
March 28, 2012 12:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2317 2317
Size: 6220
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4030 278
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3563 1937
Size: 1626 4594
Acc65I G'GTAC_C
Cuts at: 2271 3208 3541 2271
Size: 937 333 4950
Fragments arranged by size:
4950 937 333
AclI AA'CG_TT
Cuts at: 1563 4347 4720 1563
Size: 2784 373 3063
Fragments arranged by size:
3063 2784 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4283 5667 5935 1632
Size: 2651 1384 268 1917
Fragments arranged by size:
2651 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6023
AflIII A'CryG_T
Cuts at: 1203 3477 5119 6096 1203
Size: 2274 1642 977 1327
Fragments arranged by size:
2274 1642 1327 977
AgeI A'CCGG_T
Cuts at: 5051 5051
Size: 6220
AhdI GACnn_n'nnGTC
Cuts at: 4949 4949
Size: 6220
AleI CACnn'nnGTG
Cuts at: 1166 3275 1166
Size: 2109 4111
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2171 1184
Size: 987 5233
AlwNI CAG_nnn'CTG
Cuts at: 3963 5535 3963
Size: 1572 4648
ApaI G_GGCC'C
Cuts at: 2051 3132 2051
Size: 1081 5139
ApaLI G'TGCA_C
Cuts at: 4277 5433 4277
Size: 1156 5064
ApoI r'AATT_y
Cuts at: 1493 2486 3529 3755 3907 6016 1493
Size: 993 1043 226 152 2109 1697
Fragments arranged by size:
2109 1697 1043 993 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4774 160
Size: 872 3742 1606
Fragments arranged by size:
3742 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
AvaI C'yCGr_G
Cuts at: 1870 1943 2050 2380 2787 2876 3545 1870
Size: 73 107 330 407 89 669 4545
Fragments arranged by size:
4545 669 407 330 107 89 73
BamHI G'GATC_C
Cuts at: 3550 3917 3550
Size: 367 5853
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2271 2300 3208 3541 4996
Size: 325 217 627 484 29 908 333 1455
Cuts at: 4996 618
Size: 1842
Fragments arranged by size:
1842 1455 908 627 484 333 325 217
29
BanII G_rGCy'C
Cuts at: 1391 2051 3132 3539 1391
Size: 660 1081 407 4072
Fragments arranged by size:
4072 1081 660 407
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3513 928
Size: 971 1614 3635
Fragments arranged by size:
3635 1614 971
BbvCI CC'TCA_GC
Cuts at: 2593 2593
Size: 6220
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4411 4445 1903
Size: 34 2474 34 3678
Fragments arranged by size:
3678 2474 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4112 5322 4112
Size: 1210 5010
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6220
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2768 4831 136
Size: 107 122 71 631 605 1096 2063 1525
Fragments arranged by size:
2063 1525 1096 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6215 6215
Size: 6220
BlpI GC'TnA_GC
Cuts at: 3637 3637
Size: 6220
Bme1580I G_kGCm'C
Cuts at: 1790 2031 2051 3132 4281 5437 1790
Size: 241 20 1081 1149 1156 2573
Fragments arranged by size:
2573 1156 1149 1081 241 20
BmgBI CAC'GTC
Cuts at: 1318 2344 1318
Size: 1026 5194
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3951 4909 442
Size: 416 53 83 399 24 2534 958 1753
Fragments arranged by size:
2534 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6220
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2302 2608 2302
Size: 306 5914
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2717 2726 3533 4880 1239
Size: 1478 9 807 1347 2579
Fragments arranged by size:
2579 1478 1347 807 9
Bpu10I CC'TnA_GC
Cuts at: 2579 2593 2579
Size: 14 6206
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2402 3425 4345 5210 5508 2402
Size: 1023 920 865 298 3114
Fragments arranged by size:
3114 1023 920 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2924 4883 882
Size: 617 1425 1959 2219
Fragments arranged by size:
2219 1959 1425 617
BsaAI yAC'GTr
Cuts at: 493 1204 4011 493
Size: 711 2807 2702
Fragments arranged by size:
2807 2702 711
BsaBI GATnn'nnATC
Cuts at: 6214 6214
Size: 6220
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4027 4409 5044 275
Size: 53 83 186 936 2494 382 635 1451
Fragments arranged by size:
2494 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4653 5051 5325 5472 5883 4653
Size: 398 274 147 411 4990
Fragments arranged by size:
4990 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3224 3254 1178
Size: 30 165 30 1821 30 4144
Fragments arranged by size:
4144 1821 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2519 2522 1186
Size: 1333 3 4884
Fragments arranged by size:
4884 1333 3
BseYI C'CCAG_C
Cuts at: 2394 2837 2975 3074 5423 5919 2394
Size: 443 138 99 2349 496 2695
Fragments arranged by size:
2695 2349 496 443 138 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2287 1163
Size: 573 551 5096
Fragments arranged by size:
5096 573 551
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2045 3670 4431 5459 1540
Size: 275 186 44 1625 761 1028 2301
Fragments arranged by size:
2301 1625 1028 761 275 186 44
BsiHKAI G_wGCw'C
Cuts at: 4281 4366 5437 4281
Size: 85 1071 5064
Fragments arranged by size:
5064 1071 85
BsmI GAATG_Cn'
Cuts at: 3731 3824 3731
Size: 93 6127
BsmBI CGTCTCn'nnnn_
Cuts at: 2257 2257
Size: 6220
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2051 3132 3539 4281 4366 5437
Size: 399 241 20 1081 407 742 85 1071
Cuts at: 5437 1391
Size: 2174
Fragments arranged by size:
2174 1081 1071 742 407 399 241 85
20
BspEI T'CCGG_A
Cuts at: 5883 5883
Size: 6220
BspHI T'CATG_A
Cuts at: 4109 5839 4109
Size: 1730 4490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2259 2317 3575 844
Size: 1415 58 1258 3489
Fragments arranged by size:
3489 1415 1258 58
BsrBI CCG'CTC
Cuts at: 1394 1823 4107 1394
Size: 429 2284 3507
Fragments arranged by size:
3507 2284 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3166 4715 4889 65
Size: 1183 1918 1549 174 1396
Fragments arranged by size:
1918 1549 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2018 4864 5051 1700
Size: 110 208 2846 187 2869
Fragments arranged by size:
2869 2846 208 187 110
BsrGI T'GTAC_A
Cuts at: 96 3019 96
Size: 2923 3297
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6220
BssSI C'ACGA_G
Cuts at: 4280 5292 4280
Size: 1012 5208
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6220
BstBI TT'CG_AA
Cuts at: 5024 5024
Size: 6220
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6220
BstXI CCAn_nnnn'nTGG
Cuts at: 2282 2282
Size: 6220
BstYI r'GATC_y
Cuts at: 1972 2259 2971 3550 3917 4302 4319 5771 5857
Size: 287 712 579 367 385 17 1452 86
Cuts at: 5857 5869 6215 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 712 579 385 367 346 287
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3174 3174
Size: 6220
BtgI C'CryG_G
Cuts at: 513 1065 2197 2242 3199 3273 513
Size: 552 1132 45 957 74 3460
Fragments arranged by size:
3460 1132 957 552 74 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5481
Fragments arranged by size:
5481 683 56
BtsI GCAGTG_nn'
Cuts at: 2892 3499 3736 4530 4550 2892
Size: 607 237 794 20 4562
Fragments arranged by size:
4562 794 607 237 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6220
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3184 3219 3475 3510 529
Size: 35 2620 35 256 35 3239
Fragments arranged by size:
3239 2620 256 35 35 35
DraI TTT'AAA
Cuts at: 3524 3524
Size: 6220
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5227 809
Size: 507 3911 1802
Fragments arranged by size:
3911 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2042 2104
Size: 54 1425 50 275 18 186 26 62
Cuts at: 2104 3282 3667 4556 6185 8
Size: 1178 385 889 1629 43
Fragments arranged by size:
1629 1425 1178 889 385 275 186 62
54 50 43 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 2042 3667 1537
Size: 275 230 1625 4090
Fragments arranged by size:
4090 1625 275 230
EarI CTCTTCn'nnn_
Cuts at: 1353 2496 3308 3495 4150 1353
Size: 1143 812 187 655 3423
Fragments arranged by size:
3423 1143 812 655 187
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2159 4807 5181 5327 387
Size: 1064 138 570 2648 374 146 1280
Fragments arranged by size:
2648 1280 1064 570 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2717 2726 3533 4283 4880 5667 5935
Size: 393 1085 9 807 750 597 787 268
Cuts at: 5935 1239
Size: 1524
Fragments arranged by size:
1524 1085 807 787 750 597 393 268
9
EcoNI CCTnn'n_nnAGG
Cuts at: 2105 3393 2105
Size: 1288 4932
EcoO109I rG'GnC_Cy
Cuts at: 3128 3129 3128
Size: 1 6219
EcoRI G'AATT_C
Cuts at: 3529 3907 3529
Size: 378 5842
EcoRV GAT'ATC
Cuts at: 3451 3451
Size: 6220
FspI TGC'GCA
Cuts at: 4726 4726
Size: 6220
HaeII r_GCGC'y
Cuts at: 1678 2025 5367 1678
Size: 347 3342 2531
Fragments arranged by size:
3342 2531 347
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2600 2612 2632 2644 2781
Size: 32 135 32 1028 12 20 12 137
Cuts at: 2781 2813 3041 3073 4861 4893 4935 4967 1373
Size: 32 228 32 1788 32 42 32 2626
Fragments arranged by size:
2626 1788 1028 228 137 135 42 32
32 32 32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2119 3564 3810 669
Size: 988 281 181 1445 246 3079
Fragments arranged by size:
3079 1445 988 281 246 181
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6220
HpaI GTT'AAC
Cuts at: 3810 3810
Size: 6220
KpnI G_GTAC'C
Cuts at: 2275 3212 3545 2275
Size: 937 333 4950
Fragments arranged by size:
4950 937 333
MfeI C'AATT_G
Cuts at: 3819 3819
Size: 6220
MluI A'CGCG_T
Cuts at: 6096 6096
Size: 6220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2907 3066 3159 5334 5518 1305
Size: 1602 159 93 2175 184 2007
Fragments arranged by size:
2175 2007 1602 184 159 93
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4398
Fragments arranged by size:
4398 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2897 3275 4178 4537 4696 518
Size: 648 1731 378 903 359 159 2042
Fragments arranged by size:
2042 1731 903 648 378 359 159
MspA1I CmG'CkG
Cuts at: 1606 2083 2155 2230 2394 2695 2923 3288 3636
Size: 477 72 75 164 301 228 365 348
Cuts at: 3636 4313 5461 5706 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 477 365 348 301 245
228 164 75 72
NaeI GCC'GGC
Cuts at: 2020 2020
Size: 6220
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5668
NdeI CA'TA_TG
Cuts at: 387 3309 387
Size: 2922 3298
NgoMIV G'CCGG_C
Cuts at: 2018 2018
Size: 6220
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6220
NotI GC'GGCC_GC
Cuts at: 3667 3667
Size: 6220
NspI r_CATG'y
Cuts at: 1132 1229 2389 3578 5123 1132
Size: 97 1160 1189 1545 2229
Fragments arranged by size:
2229 1545 1189 1160 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6220
PciI A'CATG_T
Cuts at: 5119 5119
Size: 6220
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6220
PmeI GTTT'AAAC
Cuts at: 3524 3524
Size: 6220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4289 4321 5802 5834 4289
Size: 32 1481 32 4675
Fragments arranged by size:
4675 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6220
PsiI TTA'TAA
Cuts at: 3790 3790
Size: 6220
PspOMI G'GGCC_C
Cuts at: 2047 3128 2047
Size: 1081 5139
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2625 2657 2625
Size: 32 6188
PstI C_TGCA'G
Cuts at: 830 3293 3572 830
Size: 2463 279 3478
Fragments arranged by size:
3478 2463 279
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6220
PvuII CAG'CTG
Cuts at: 2230 2394 2695 2923 3288 2230
Size: 164 301 228 365 5162
Fragments arranged by size:
5162 365 301 228 164
SalI G'TCGA_C
Cuts at: 1936 3562 1936
Size: 1626 4594
SapI GCTCTTCn'nnn_
Cuts at: 3308 3308
Size: 6220
SbfI CC_TGCA'GG
Cuts at: 3572 3572
Size: 6220
ScaI AGT'ACT
Cuts at: 4468 6180 4468
Size: 1712 4508
SfcI C'TryA_G
Cuts at: 826 1045 3289 3361 3376 3568 4703 5384 5575
Size: 219 2244 72 15 192 1135 681 191
Cuts at: 5575 826
Size: 1471
Fragments arranged by size:
2244 1471 1135 681 219 192 191 72
15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6220
SmaI CCC'GGG
Cuts at: 1872 2052 2382 2878 3547 1872
Size: 180 330 496 669 4545
Fragments arranged by size:
4545 669 496 330 180
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2417 3440 4324 5225 5487 820
Size: 197 926 474 1023 884 901 262 1553
Fragments arranged by size:
1553 1023 926 901 884 474 262 197
SnaBI TAC'GTA
Cuts at: 493 4011 493
Size: 3518 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6220
SphI G_CATG'C
Cuts at: 1132 3578 1132
Size: 2446 3774
SspI AAT'ATT
Cuts at: 5 52 4144 5
Size: 47 4092 2081
Fragments arranged by size:
4092 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6220
StyI C'CwwG_G
Cuts at: 513 1065 3659 513
Size: 552 2594 3074
Fragments arranged by size:
3074 2594 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3370 4247 4264 4417 4602 5047 325
Size: 1255 1790 877 17 153 185 445 1498
Fragments arranged by size:
1790 1498 1255 877 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2791 3019 4466
Size: 274 80 33 51 1443 814 228 1447
Cuts at: 4466 6178 96
Size: 1712 138
Fragments arranged by size:
1712 1447 1443 814 274 228 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2938 2970 6180 6212 2938
Size: 32 3210 32 2946
Fragments arranged by size:
3210 2946 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2426 3389 1316
Size: 1110 963 4147
Fragments arranged by size:
4147 1110 963
XbaI T'CTAG_A
Cuts at: 3556 3556
Size: 6220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2851 3453 2011
Size: 840 602 4778
Fragments arranged by size:
4778 840 602
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6220
XmaI C'CCGG_G
Cuts at: 1870 2050 2380 2876 3545 1870
Size: 180 330 496 669 4545
Fragments arranged by size:
4545 669 496 330 180
XmnI GAAnn'nnTTC
Cuts at: 3479 4349 3479
Size: 870 5350
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4028 276
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I DraIII EcoICRI FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PpuMI PspXI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI