Restriction map for
pFN21AB5920
(Circular) (Six-base) MAPSORT of: pFN21AB5920.seq Check: 760 from: 1 to: 6301
pFN21AB5920 6301 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2354 2826 2993 3432 2354
Size: 472 167 439 5223
Fragments arranged by size:
5223 472 439 167
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4111 278
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3357 3644 1937
Size: 1420 287 4594
Fragments arranged by size:
4594 1420 287
Acc65I G'GTAC_C
Cuts at: 3622 3622
Size: 6301
AclI AA'CG_TT
Cuts at: 1563 4428 4801 1563
Size: 2865 373 3063
Fragments arranged by size:
3063 2865 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3466 4364 5748 6016 1632
Size: 1834 898 1384 268 1917
Fragments arranged by size:
1917 1834 1384 898 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6104
AflIII A'CryG_T
Cuts at: 1203 2382 2850 5200 6177 1203
Size: 1179 468 2350 977 1327
Fragments arranged by size:
2350 1327 1179 977 468
AgeI A'CCGG_T
Cuts at: 5132 5132
Size: 6301
AhdI GACnn_n'nnGTC
Cuts at: 2488 5030 2488
Size: 2542 3759
AleI CACnn'nnGTG
Cuts at: 1166 2385 3590 1166
Size: 1219 1205 3877
Fragments arranged by size:
3877 1219 1205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6301
AlwNI CAG_nnn'CTG
Cuts at: 2066 4044 5616 2066
Size: 1978 1572 2751
Fragments arranged by size:
2751 1978 1572
ApaI G_GGCC'C
Cuts at: 2264 2264
Size: 6301
ApaLI G'TGCA_C
Cuts at: 4358 5514 4358
Size: 1156 5145
ApoI r'AATT_y
Cuts at: 1493 3610 3836 3988 6097 1493
Size: 2117 226 152 2109 1697
Fragments arranged by size:
2117 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4855 160
Size: 872 3823 1606
Fragments arranged by size:
3823 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
AvaI C'yCGr_G
Cuts at: 1870 1943 2255 3168 3626 1870
Size: 73 312 913 458 4545
Fragments arranged by size:
4545 913 458 312 73
BamHI G'GATC_C
Cuts at: 2806 3631 3998 2806
Size: 825 367 5109
Fragments arranged by size:
5109 825 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2366 2795 2814 3622 5077
Size: 325 217 627 579 429 19 808 1455
Cuts at: 5077 618
Size: 1842
Fragments arranged by size:
1842 1455 808 627 579 429 325 217
19
BanII G_rGCy'C
Cuts at: 1391 2226 2249 2264 2410 2585 3086 3620 1391
Size: 835 23 15 146 175 501 534 4072
Fragments arranged by size:
4072 835 534 501 175 146 23 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2055 2536 2661 3224 928
Size: 971 156 481 125 563 4005
Fragments arranged by size:
4005 971 563 481 156 125
BbvCI CC'TCA_GC
Cuts at: 3233 3233
Size: 6301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2800 2834 4492 4526 1903
Size: 34 863 34 1658 34 3678
Fragments arranged by size:
3678 1658 863 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4193 5403 4193
Size: 1210 5091
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6301
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2772 4912 136
Size: 107 122 71 631 605 1100 2140 1525
Fragments arranged by size:
2140 1525 1100 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6296 6296
Size: 6301
BlpI GC'TnA_GC
Cuts at: 3718 3718
Size: 6301
Bme1580I G_kGCm'C
Cuts at: 1790 2264 2798 4362 5518 1790
Size: 474 534 1564 1156 2573
Fragments arranged by size:
2573 1564 1156 534 474
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6301
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4032 4990 442
Size: 416 53 83 399 24 2615 958 1753
Fragments arranged by size:
2615 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2251 2486 2602 3212 3229 4961 1239
Size: 1012 235 116 610 17 1732 2579
Fragments arranged by size:
2579 1732 1012 610 235 116 17
Bpu10I CC'TnA_GC
Cuts at: 2220 3233 2220
Size: 1013 5288
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2437 3115 4426 5291 5589 2437
Size: 678 1311 865 298 3149
Fragments arranged by size:
3149 1311 865 678 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2420 2620 4964 882
Size: 617 921 200 2344 2219
Fragments arranged by size:
2344 2219 921 617 200
BsaAI yAC'GTr
Cuts at: 493 1204 2383 2827 2857 4092 493
Size: 711 1179 444 30 1235 2702
Fragments arranged by size:
2702 1235 1179 711 444 30
BsaBI GATnn'nnATC
Cuts at: 6295 6295
Size: 6301
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4108 4490 5125 275
Size: 53 83 186 936 2575 382 635 1451
Fragments arranged by size:
2575 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2044 2547 4734 5132 5406 5553 5964 2044
Size: 503 2187 398 274 147 411 2381
Fragments arranged by size:
2381 2187 503 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6076
Fragments arranged by size:
6076 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 2186 2189 3209 3335 3476 3479 1186
Size: 943 57 3 1020 126 141 3 4008
Fragments arranged by size:
4008 1020 943 141 126 57 3 3
BseYI C'CCAG_C
Cuts at: 2059 2130 3146 5504 6000 2059
Size: 71 1016 2358 496 2360
Fragments arranged by size:
2360 2358 1016 496 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2420 2525 3044 3291 3568 1163
Size: 573 684 105 519 247 277 3896
Fragments arranged by size:
3896 684 573 519 277 247 105
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3751 4512 5540 1540
Size: 275 186 1750 761 1028 2301
Fragments arranged by size:
2301 1750 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2226 2410 2585 4362 4447 5518 2226
Size: 184 175 1777 85 1071 3009
Fragments arranged by size:
3009 1777 1071 184 175 85
BsmI GAATG_Cn'
Cuts at: 2683 3812 3905 2683
Size: 1129 93 5079
Fragments arranged by size:
5079 1129 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2249 2264 2410 2585 2798 3086
Size: 399 436 23 15 146 175 213 288
Cuts at: 3086 3620 4362 4447 5518 1391
Size: 534 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 534 436 399 288 213
175 146 85 23 15
BspEI T'CCGG_A
Cuts at: 2044 2547 5964 2044
Size: 503 3417 2381
Fragments arranged by size:
3417 2381 503
BspHI T'CATG_A
Cuts at: 4190 5920 4190
Size: 1730 4571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2334 2354 2826 2993 3432 3525 3656 844
Size: 1490 20 472 167 439 93 131 3489
Fragments arranged by size:
3489 1490 472 439 167 131 93 20
BsrBI CCG'CTC
Cuts at: 1394 1823 2470 4188 1394
Size: 429 647 1718 3507
Fragments arranged by size:
3507 1718 647 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3577 4796 4970 65
Size: 1183 2329 1219 174 1396
Fragments arranged by size:
2329 1396 1219 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2153 4945 5132 1700
Size: 110 343 2792 187 2869
Fragments arranged by size:
2869 2792 343 187 110
BsrGI T'GTAC_A
Cuts at: 96 2322 3030 96
Size: 2226 708 3367
Fragments arranged by size:
3367 2226 708
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6301
BssSI C'ACGA_G
Cuts at: 2486 3259 3527 4361 5373 2486
Size: 773 268 834 1012 3414
Fragments arranged by size:
3414 1012 834 773 268
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2366 794
Size: 1572 4729
BstBI TT'CG_AA
Cuts at: 2929 5105 2929
Size: 2176 4125
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6301
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6301
BstYI r'GATC_y
Cuts at: 1972 2014 2697 2806 2952 3631 3998 4383 4400
Size: 42 683 109 146 679 367 385 17
Cuts at: 4400 5852 5938 5950 6296 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 683 679 385 367 346 146
109 86 42 17 12
Bsu36I CC'TnA_GG
Cuts at: 2112 2112
Size: 6301
BtgI C'CryG_G
Cuts at: 513 1065 2004 2493 2520 3126 3301 3550 513
Size: 552 939 489 27 606 175 249 3264
Fragments arranged by size:
3264 939 606 552 489 249 175 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2307 487
Size: 683 56 1081 4481
Fragments arranged by size:
4481 1081 683 56
BtsI GCAGTG_nn'
Cuts at: 2968 3817 4611 4631 2968
Size: 849 794 20 4638
Fragments arranged by size:
4638 849 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6301
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6266
DraI TTT'AAA
Cuts at: 3605 3605
Size: 6301
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5308 809
Size: 507 3992 1802
Fragments arranged by size:
3992 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2935 3123 3139
Size: 54 1425 50 275 18 1105 188 16
Cuts at: 3139 3496 3748 4637 6266 8
Size: 357 252 889 1629 43
Fragments arranged by size:
1629 1425 1105 889 357 275 252 188
54 50 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 3748 1537
Size: 275 1936 4090
Fragments arranged by size:
4090 1936 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3014 4231 1353
Size: 1661 1217 3423
Fragments arranged by size:
3423 1661 1217
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2541 2993 4888 5262 5408 387
Size: 1064 138 952 452 1895 374 146 1280
Fragments arranged by size:
1895 1280 1064 952 452 374 146 138
EcoICRI GAG'CTC
Cuts at: 2224 2408 2583 2224
Size: 184 175 5942
Fragments arranged by size:
5942 184 175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2251 2486 2602 3212 3229 3466 4364
Size: 393 619 235 116 610 17 237 898
Cuts at: 4364 4961 5748 6016 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 898 787 619 610 597 393 268
237 235 116 17
EcoNI CCTnn'n_nnAGG
Cuts at: 3194 3194
Size: 6301
EcoO109I rG'GnC_Cy
Cuts at: 2070 2127 2260 2261 2337 2070
Size: 57 133 1 76 6034
Fragments arranged by size:
6034 133 76 57 1
EcoRI G'AATT_C
Cuts at: 3610 3988 3610
Size: 378 5923
FspI TGC'GCA
Cuts at: 4807 4807
Size: 6301
HaeII r_GCGC'y
Cuts at: 1678 5448 1678
Size: 3770 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2474 2506 2760 2792 4942
Size: 32 135 32 902 32 254 32 2150
Cuts at: 4942 4974 5016 5048 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2150 902 254 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3645 3891 669
Size: 988 281 1707 246 3079
Fragments arranged by size:
3079 1707 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2735 2924 748
Size: 1987 189 4125
Fragments arranged by size:
4125 1987 189
HpaI GTT'AAC
Cuts at: 3891 3891
Size: 6301
KpnI G_GTAC'C
Cuts at: 3626 3626
Size: 6301
MfeI C'AATT_G
Cuts at: 3900 3900
Size: 6301
MluI A'CGCG_T
Cuts at: 6177 6177
Size: 6301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2871 5415 5599 1305
Size: 1566 2544 184 2007
Fragments arranged by size:
2544 2007 1566 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2937 3125 3141 3498 10
Size: 54 1425 343 1105 188 16 357 2813
Fragments arranged by size:
2813 1425 1105 357 343 188 54 16
MslI CAynn'nnrTG
Cuts at: 518 1166 2385 3590 4259 4618 4777 518
Size: 648 1219 1205 669 359 159 2042
Fragments arranged by size:
2042 1219 1205 669 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2622 2716 3028 3331 3388 3544 3552 3717
Size: 1016 94 312 303 57 156 8 165
Cuts at: 3717 4394 5542 5787 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1016 677 312 303 245 165
156 94 57 8
NcoI C'CATG_G
Cuts at: 513 1065 2004 3126 513
Size: 552 939 1122 3688
Fragments arranged by size:
3688 1122 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6301
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6301
NotI GC'GGCC_GC
Cuts at: 3748 3748
Size: 6301
NspI r_CATG'y
Cuts at: 1132 1229 3659 5204 1132
Size: 97 2430 1545 2229
Fragments arranged by size:
2430 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2066 3397 1348
Size: 718 1331 4252
Fragments arranged by size:
4252 1331 718
PciI A'CATG_T
Cuts at: 5200 5200
Size: 6301
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2066 1108
Size: 958 5343
PfoI T'CCnGG_A
Cuts at: 3375 3375
Size: 6301
PmeI GTTT'AAAC
Cuts at: 3605 3605
Size: 6301
PmlI CAC'GTG
Cuts at: 2383 2383
Size: 6301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4370 4402 5883 5915 4370
Size: 32 1481 32 4756
Fragments arranged by size:
4756 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2070 2127 2070
Size: 57 6244
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6301
PsiI TTA'TAA
Cuts at: 3871 3871
Size: 6301
PspOMI G'GGCC_C
Cuts at: 2260 2260
Size: 6301
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2558 2590 2558
Size: 32 6269
PstI C_TGCA'G
Cuts at: 830 2231 2345 2715 2823 2963 3429 3525 3653
Size: 1401 114 370 108 140 466 96 128
Cuts at: 3653 830
Size: 3478
Fragments arranged by size:
3478 1401 466 370 140 128 114 108
96
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6301
PvuII CAG'CTG
Cuts at: 2622 2716 3028 3544 2622
Size: 94 312 516 5379
Fragments arranged by size:
5379 516 312 94
SacI G_AGCT'C
Cuts at: 2226 2410 2585 2226
Size: 184 175 5942
Fragments arranged by size:
5942 184 175
SacII CC_GC'GG
Cuts at: 3553 3553
Size: 6301
SalI G'TCGA_C
Cuts at: 1936 3643 1936
Size: 1707 4594
SanDI GG'GwC_CC
Cuts at: 2070 2070
Size: 6301
SbfI CC_TGCA'GG
Cuts at: 2345 3653 2345
Size: 1308 4993
ScaI AGT'ACT
Cuts at: 4549 6261 4549
Size: 1712 4589
SfcI C'TryA_G
Cuts at: 826 1045 2227 2341 2711 2819 2959 3425 3521
Size: 219 1182 114 370 108 140 466 96
Cuts at: 3521 3649 4784 5465 5656 826
Size: 128 1135 681 191 1471
Fragments arranged by size:
1471 1182 1135 681 466 370 219 191
140 128 114 108 96
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6301
SmaI CCC'GGG
Cuts at: 1872 3170 3628 1872
Size: 1298 458 4545
Fragments arranged by size:
4545 1298 458
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2416 3094 4405 5306 5568 820
Size: 197 926 473 678 1311 901 262 1553
Fragments arranged by size:
1553 1311 926 901 678 473 262 197
SnaBI TAC'GTA
Cuts at: 493 4092 493
Size: 3599 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6301
SphI G_CATG'C
Cuts at: 1132 3659 1132
Size: 2527 3774
SspI AAT'ATT
Cuts at: 5 52 4225 5
Size: 47 4173 2081
Fragments arranged by size:
4173 2081 47
StuI AGG'CCT
Cuts at: 1517 3206 1517
Size: 1689 4612
StyI C'CwwG_G
Cuts at: 513 1065 2004 2790 3126 3740 513
Size: 552 939 786 336 614 3074
Fragments arranged by size:
3074 939 786 614 552 336
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3302 4328 4345 4498 4683 5128 325
Size: 1255 1722 1026 17 153 185 445 1498
Fragments arranged by size:
1722 1498 1255 1026 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2322 3030 3477
Size: 274 80 33 51 1443 345 708 447
Cuts at: 3477 4547 6259 96
Size: 1070 1712 138
Fragments arranged by size:
1712 1443 1070 708 447 345 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6261 6293 6261
Size: 32 6269
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6301
XbaI T'CTAG_A
Cuts at: 3637 3637
Size: 6301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2854 3335 2854
Size: 481 5820
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6301
XmaI C'CCGG_G
Cuts at: 1870 3168 3626 1870
Size: 1298 458 4545
Fragments arranged by size:
4545 1298 458
XmnI GAAnn'nnTTC
Cuts at: 2555 4430 2555
Size: 1875 4426
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4109 276
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SacII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI
RsrII SapI SexAI SfiI SfoI SrfI SwaI