Restriction map for
pFN21AB5919
(Circular) (Six-base) MAPSORT of: pFN21AB5919.seq Check: 9658 from: 1 to: 6220
pFN21AB5919 6220 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2189 2189
Size: 6220
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4030 278
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3000 3563 1937
Size: 1063 563 4594
Fragments arranged by size:
4594 1063 563
Acc65I G'GTAC_C
Cuts at: 3541 3541
Size: 6220
AclI AA'CG_TT
Cuts at: 1563 4347 4720 1563
Size: 2784 373 3063
Fragments arranged by size:
3063 2784 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2735 4283 5667 5935 1632
Size: 1103 1548 1384 268 1917
Fragments arranged by size:
1917 1548 1384 1103 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6023
AflIII A'CryG_T
Cuts at: 1203 5119 6096 1203
Size: 3916 977 1327
Fragments arranged by size:
3916 1327 977
AgeI A'CCGG_T
Cuts at: 2414 5051 2414
Size: 2637 3583
AhdI GACnn_n'nnGTC
Cuts at: 4949 4949
Size: 6220
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6220
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6220
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2034 2066 2034
Size: 32 6188
AlwNI CAG_nnn'CTG
Cuts at: 2713 3963 5535 2713
Size: 1250 1572 3398
Fragments arranged by size:
3398 1572 1250
ApaI G_GGCC'C
Cuts at: 2107 2107
Size: 6220
ApaLI G'TGCA_C
Cuts at: 4277 5433 4277
Size: 1156 5064
ApoI r'AATT_y
Cuts at: 1493 2456 2567 3230 3529 3755 3907 6016 1493
Size: 963 111 663 299 226 152 2109 1697
Fragments arranged by size:
2109 1697 963 663 299 226 152 111
AseI AT'TA_AT
Cuts at: 160 1032 4774 160
Size: 872 3742 1606
Fragments arranged by size:
3742 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
AvaI C'yCGr_G
Cuts at: 1870 1943 3545 1870
Size: 73 1602 4545
Fragments arranged by size:
4545 1602 73
BamHI G'GATC_C
Cuts at: 2812 3550 3917 2812
Size: 738 367 5115
Fragments arranged by size:
5115 738 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3320 3541 4996 618
Size: 325 217 627 1533 221 1455 1842
Fragments arranged by size:
1842 1533 1455 627 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2107 3539 1391
Size: 716 1432 4072
Fragments arranged by size:
4072 1432 716
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3162 3474 928
Size: 971 1263 312 3674
Fragments arranged by size:
3674 1263 971 312
BbvCI CC'TCA_GC
Cuts at: 2178 2322 2178
Size: 144 6076
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4411 4445 1903
Size: 34 2474 34 3678
Fragments arranged by size:
3678 2474 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4112 5322 4112
Size: 1210 5010
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6220
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4831 136
Size: 107 122 71 631 605 3159 1525
Fragments arranged by size:
3159 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3064 6215 3064
Size: 3151 3069
BlpI GC'TnA_GC
Cuts at: 3637 3637
Size: 6220
Bme1580I G_kGCm'C
Cuts at: 1790 2107 3325 4281 5437 1790
Size: 317 1218 956 1156 2573
Fragments arranged by size:
2573 1218 1156 956 317
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6220
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2113 3951 4909
Size: 416 53 83 399 24 696 1838 958
Cuts at: 4909 442
Size: 1753
Fragments arranged by size:
1838 1753 958 696 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2263 2760 3059 4880 1239
Size: 1024 497 299 1821 2579
Fragments arranged by size:
2579 1821 1024 497 299
Bpu10I CC'TnA_GC
Cuts at: 2178 2322 2178
Size: 144 6076
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2206 4345 5210 5508 2206
Size: 2139 865 298 2918
Fragments arranged by size:
2918 2139 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4883 882
Size: 617 3384 2219
Fragments arranged by size:
3384 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2317 4011 493
Size: 711 1113 1694 2702
Fragments arranged by size:
2702 1694 1113 711
BsaBI GATnn'nnATC
Cuts at: 2725 6214 2725
Size: 3489 2731
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4027 4409 5044 275
Size: 53 83 186 936 2494 382 635 1451
Fragments arranged by size:
2494 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2414 4653 5051 5325 5472 5883 2414
Size: 2239 398 274 147 411 2751
Fragments arranged by size:
2751 2239 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2034 2064 2472 2502 2684
Size: 30 165 30 631 30 408 30 182
Cuts at: 2684 2714 1178
Size: 30 4684
Fragments arranged by size:
4684 631 408 182 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6220
BseYI C'CCAG_C
Cuts at: 2614 2644 5423 5919 2614
Size: 30 2779 496 2915
Fragments arranged by size:
2915 2779 496 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5647
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3670 4431 5459 1540
Size: 275 186 1669 761 1028 2301
Fragments arranged by size:
2301 1669 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4281 4366 5437 4281
Size: 85 1071 5064
Fragments arranged by size:
5064 1071 85
BsmI GAATG_Cn'
Cuts at: 3421 3731 3824 3421
Size: 310 93 5817
Fragments arranged by size:
5817 310 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2107 3325 3539 4281 4366 5437 1391
Size: 399 317 1218 214 742 85 1071 2174
Fragments arranged by size:
2174 1218 1071 742 399 317 214 85
BspEI T'CCGG_A
Cuts at: 5883 5883
Size: 6220
BspHI T'CATG_A
Cuts at: 4109 5839 4109
Size: 1730 4490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2189 3575 844
Size: 1345 1386 3489
Fragments arranged by size:
3489 1386 1345
BsrBI CCG'CTC
Cuts at: 1394 1823 4107 1394
Size: 429 2284 3507
Fragments arranged by size:
3507 2284 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4715 4889 65
Size: 1183 3467 174 1396
Fragments arranged by size:
3467 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2414 3190 4864 5051 1700
Size: 110 604 776 1674 187 2869
Fragments arranged by size:
2869 1674 776 604 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6220
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6220
BssSI C'ACGA_G
Cuts at: 4280 5292 4280
Size: 1012 5208
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3017 794
Size: 2223 3997
BstBI TT'CG_AA
Cuts at: 5024 5024
Size: 6220
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6220
BstXI CCAn_nnnn'nTGG
Cuts at: 2257 2257
Size: 6220
BstYI r'GATC_y
Cuts at: 1972 2634 2726 2812 2902 3064 3550 3917 4302
Size: 662 92 86 90 162 486 367 385
Cuts at: 4302 4319 5771 5857 5869 6215 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 662 486 385 367 346 162
92 90 86 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2828 3426 2828
Size: 598 5622
BtgI C'CryG_G
Cuts at: 513 1065 2064 2348 2673 513
Size: 552 999 284 325 4060
Fragments arranged by size:
4060 999 552 325 284
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5481
Fragments arranged by size:
5481 683 56
BtsI GCAGTG_nn'
Cuts at: 2790 3449 3736 4530 4550 2790
Size: 659 287 794 20 4460
Fragments arranged by size:
4460 794 659 287 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6220
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6185
DraI TTT'AAA
Cuts at: 3524 3524
Size: 6220
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2940 5227 809
Size: 507 1624 2287 1802
Fragments arranged by size:
2287 1802 1624 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2134 3667 4556
Size: 54 1425 50 275 18 304 1533 889
Cuts at: 4556 6185 8
Size: 1629 43
Fragments arranged by size:
1629 1533 1425 889 304 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3667 1537
Size: 275 1855 4090
Fragments arranged by size:
4090 1855 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3493 4150 1353
Size: 2140 657 3423
Fragments arranged by size:
3423 2140 657
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4807 5181 5327 387
Size: 1064 138 3218 374 146 1280
Fragments arranged by size:
3218 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2263 2735 2760 3059 4283 4880 5667
Size: 393 631 472 25 299 1224 597 787
Cuts at: 5667 5935 1239
Size: 268 1524
Fragments arranged by size:
1524 1224 787 631 597 472 393 299
268 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2831 2831
Size: 6220
EcoO109I rG'GnC_Cy
Cuts at: 2103 2104 2641 2103
Size: 1 537 5682
Fragments arranged by size:
5682 537 1
EcoRI G'AATT_C
Cuts at: 2456 3529 3907 2456
Size: 1073 378 4769
Fragments arranged by size:
4769 1073 378
FspI TGC'GCA
Cuts at: 4726 4726
Size: 6220
HaeII r_GCGC'y
Cuts at: 1678 5367 1678
Size: 3689 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2080 2112 2517 2549 2684
Size: 32 135 32 508 32 405 32 135
Cuts at: 2684 2716 2957 2964 2989 2996 3239 3271 3328
Size: 32 241 7 25 7 243 32 57
Cuts at: 3328 3360 4861 4893 4935 4967 1373
Size: 32 1501 32 42 32 2626
Fragments arranged by size:
2626 1501 508 405 243 241 135 135
57 42 32 32 32 32 32 32
32 32 32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2082 3564 3810 669
Size: 988 281 144 1482 246 3079
Fragments arranged by size:
3079 1482 988 281 246 144
HindIII A'AGCT_T
Cuts at: 748 2867 748
Size: 2119 4101
HpaI GTT'AAC
Cuts at: 3810 3810
Size: 6220
KpnI G_GTAC'C
Cuts at: 3545 3545
Size: 6220
MfeI C'AATT_G
Cuts at: 3819 3819
Size: 6220
MluI A'CGCG_T
Cuts at: 6096 6096
Size: 6220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3384 3396 3437 5334 5518 1305
Size: 2079 12 41 1897 184 2007
Fragments arranged by size:
2079 2007 1897 184 41 12
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2136 10
Size: 54 1425 343 304 4094
Fragments arranged by size:
4094 1425 343 304 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4178 4537 4696 518
Size: 648 3012 359 159 2042
Fragments arranged by size:
3012 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3636 4313 5461 5706 1606
Size: 2030 677 1148 245 2120
Fragments arranged by size:
2120 2030 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5668
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6220
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6220
NotI GC'GGCC_GC
Cuts at: 3667 3667
Size: 6220
NspI r_CATG'y
Cuts at: 1132 1229 3578 5123 1132
Size: 97 2349 1545 2229
Fragments arranged by size:
2349 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6220
PciI A'CATG_T
Cuts at: 5119 5119
Size: 6220
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3340 1108
Size: 2232 3988
PmeI GTTT'AAAC
Cuts at: 3524 3524
Size: 6220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2034 2066 2336 2368 4289 4321 5802 5834 2034
Size: 32 270 32 1921 32 1481 32 2420
Fragments arranged by size:
2420 1921 1481 270 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2641 2641
Size: 6220
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6220
PsiI TTA'TAA
Cuts at: 3790 3790
Size: 6220
PspOMI G'GGCC_C
Cuts at: 2103 2103
Size: 6220
PstI C_TGCA'G
Cuts at: 830 3572 830
Size: 2742 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6220
SalI G'TCGA_C
Cuts at: 1936 3562 1936
Size: 1626 4594
SbfI CC_TGCA'GG
Cuts at: 3572 3572
Size: 6220
ScaI AGT'ACT
Cuts at: 4468 6180 4468
Size: 1712 4508
SexAI A'CCwGG_T
Cuts at: 2800 2800
Size: 6220
SfcI C'TryA_G
Cuts at: 826 1045 2336 2497 3352 3568 4703 5384 5575
Size: 219 1291 161 855 216 1135 681 191
Cuts at: 5575 826
Size: 1471
Fragments arranged by size:
1471 1291 1135 855 681 219 216 191
161
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6220
SmaI CCC'GGG
Cuts at: 1872 3547 1872
Size: 1675 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2185 4324 5225 5487 820
Size: 197 926 242 2139 901 262 1553
Fragments arranged by size:
2139 1553 926 901 262 242 197
SnaBI TAC'GTA
Cuts at: 493 4011 493
Size: 3518 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6220
SphI G_CATG'C
Cuts at: 1132 3578 1132
Size: 2446 3774
SspI AAT'ATT
Cuts at: 5 52 4144 5
Size: 47 4092 2081
Fragments arranged by size:
4092 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6220
StyI C'CwwG_G
Cuts at: 513 1065 3659 513
Size: 552 2594 3074
Fragments arranged by size:
3074 2594 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4247 4264 4417 4602 5047 325
Size: 1255 2667 17 153 185 445 1498
Fragments arranged by size:
2667 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4466 6178 96
Size: 274 80 33 51 1443 2489 1712 138
Fragments arranged by size:
2489 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2744 2776 6180 6212 2744
Size: 32 3404 32 2752
Fragments arranged by size:
3404 2752 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6220
XbaI T'CTAG_A
Cuts at: 3170 3556 3170
Size: 386 5834
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2966 2966
Size: 6220
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6220
XmaI C'CCGG_G
Cuts at: 1870 3545 1870
Size: 1675 4545
XmnI GAAnn'nnTTC
Cuts at: 2551 3216 4349 2551
Size: 665 1133 4422
Fragments arranged by size:
4422 1133 665
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4028 276
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI