Restriction map for
pFN21AB5917
(Circular) (Six-base) MAPSORT of: pFN21AB5917.seq Check: 6220 from: 1 to: 6220
pFN21AB5917 6220 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2520 4030 278
Size: 53 83 186 936 984 1510 2468
Fragments arranged by size:
2468 1510 984 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3236 3563 1937
Size: 1299 327 4594
Fragments arranged by size:
4594 1299 327
Acc65I G'GTAC_C
Cuts at: 3541 3541
Size: 6220
AclI AA'CG_TT
Cuts at: 1563 4347 4720 1563
Size: 2784 373 3063
Fragments arranged by size:
3063 2784 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2573 2614 3357 3438 4283 5667 5935 1632
Size: 941 41 743 81 845 1384 268 1917
Fragments arranged by size:
1917 1384 941 845 743 268 81 41
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6023
AflIII A'CryG_T
Cuts at: 1203 2646 3119 5119 6096 1203
Size: 1443 473 2000 977 1327
Fragments arranged by size:
2000 1443 1327 977 473
AgeI A'CCGG_T
Cuts at: 5051 5051
Size: 6220
AhdI GACnn_n'nnGTC
Cuts at: 4949 4949
Size: 6220
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6220
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2054 1184
Size: 870 5350
AlwNI CAG_nnn'CTG
Cuts at: 2848 3169 3963 5535 2848
Size: 321 794 1572 3533
Fragments arranged by size:
3533 1572 794 321
ApaI G_GGCC'C
Cuts at: 2226 2226
Size: 6220
ApaLI G'TGCA_C
Cuts at: 2680 3050 3278 4277 5433 2680
Size: 370 228 999 1156 3467
Fragments arranged by size:
3467 1156 999 370 228
ApoI r'AATT_y
Cuts at: 1493 3529 3755 3907 6016 1493
Size: 2036 226 152 2109 1697
Fragments arranged by size:
2109 2036 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2020 2020
Size: 6220
AseI AT'TA_AT
Cuts at: 160 1032 4774 160
Size: 872 3742 1606
Fragments arranged by size:
3742 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
AvaI C'yCGr_G
Cuts at: 1870 1943 2065 2156 2987 3327 3506 3545 1870
Size: 73 122 91 831 340 179 39 4545
Fragments arranged by size:
4545 831 340 179 122 91 73 39
BamHI G'GATC_C
Cuts at: 3550 3917 3550
Size: 367 5853
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2201 2216 2259 3383 3541
Size: 325 217 627 414 15 43 1124 158
Cuts at: 3541 4996 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1124 627 414 325 217 158
43 15
BanII G_rGCy'C
Cuts at: 1391 2066 2135 2163 2187 2226 2703 3074 3096
Size: 675 69 28 24 39 477 371 22
Cuts at: 3096 3539 1391
Size: 443 4072
Fragments arranged by size:
4072 675 477 443 371 69 39 28
24 22
BbeI G_GCGC'C
Cuts at: 2205 2220 2263 2205
Size: 15 43 6162
Fragments arranged by size:
6162 43 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2433 2480 3363 3457 928
Size: 971 534 47 883 94 3691
Fragments arranged by size:
3691 971 883 534 94 47
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2652 2686 4411 4445 1903
Size: 34 715 34 1725 34 3678
Fragments arranged by size:
3678 1725 715 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4112 5322 4112
Size: 1210 5010
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6220
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4831 136
Size: 107 122 71 631 605 3159 1525
Fragments arranged by size:
3159 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2352 2715 6215 2352
Size: 363 3500 2357
Fragments arranged by size:
3500 2357 363
BlpI GC'TnA_GC
Cuts at: 3312 3637 3312
Size: 325 5895
Bme1580I G_kGCm'C
Cuts at: 1790 2226 2684 2930 3054 3081 3119 3282 4281
Size: 436 458 246 124 27 38 163 999
Cuts at: 4281 5437 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 999 458 436 246 163 124
38 27
BmgBI CAC'GTC
Cuts at: 1318 2119 1318
Size: 801 5419
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2913 3951 4909
Size: 416 53 83 399 24 1496 1038 958
Cuts at: 4909 442
Size: 1753
Fragments arranged by size:
1753 1496 1038 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6220
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2737 3353 2737
Size: 616 5604
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 2237 2516 2732 4880 1239
Size: 824 174 279 216 2148 2579
Fragments arranged by size:
2579 2148 824 279 216 174
Bpu10I CC'TnA_GC
Cuts at: 3005 3005
Size: 6220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2453 4345 5210 5508 2453
Size: 1892 865 298 3165
Fragments arranged by size:
3165 1892 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2984 4883 882
Size: 617 1485 1899 2219
Fragments arranged by size:
2219 1899 1485 617
BsaAI yAC'GTr
Cuts at: 493 1204 3249 4011 493
Size: 711 2045 762 2702
Fragments arranged by size:
2702 2045 762 711
BsaBI GATnn'nnATC
Cuts at: 6214 6214
Size: 6220
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2202 2217 2260
Size: 53 83 186 936 476 193 15 43
Cuts at: 2260 2286 2517 2819 3259 4027 4409 5044 275
Size: 26 231 302 440 768 382 635 1451
Fragments arranged by size:
1451 936 768 635 476 440 382 302
231 193 186 83 53 43 26 15
BsaWI w'CCGG_w
Cuts at: 4653 5051 5325 5472 5883 4653
Size: 398 274 147 411 4990
Fragments arranged by size:
4990 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2489 2519 2690 2720 1178
Size: 30 165 30 1086 30 171 30 4678
Fragments arranged by size:
4678 1086 171 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2960 1186
Size: 1774 4446
BseYI C'CCAG_C
Cuts at: 2296 2562 2870 5423 5919 2296
Size: 266 308 2553 496 2597
Fragments arranged by size:
2597 2553 496 308 266
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2480 3229 3245 3342 3425 1163
Size: 573 744 749 16 97 83 3958
Fragments arranged by size:
3958 749 744 573 97 83 16
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2320 2559 3670 4431 5459 1540
Size: 275 186 319 239 1111 761 1028 2301
Fragments arranged by size:
2301 1111 1028 761 319 275 239 186
BsiHKAI G_wGCw'C
Cuts at: 2684 3054 3096 3282 3339 4281 4366 5437 2684
Size: 370 42 186 57 942 85 1071 3467
Fragments arranged by size:
3467 1071 942 370 186 85 57 42
BsmI GAATG_Cn'
Cuts at: 3731 3824 3731
Size: 93 6127
BsmBI CGTCTCn'nnnn_
Cuts at: 2524 2524
Size: 6220
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2135 2163 2187 2226 2684 2703
Size: 399 276 69 28 24 39 458 19
Cuts at: 2703 2930 3054 3074 3081 3096 3119 3282 3339
Size: 227 124 20 7 15 23 163 57
Cuts at: 3339 3539 4281 4366 5437 1391
Size: 200 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 458 399 276 227 200
163 124 85 69 57 39 28 24
23 20 19 15 7
BspEI T'CCGG_A
Cuts at: 5883 5883
Size: 6220
BspHI T'CATG_A
Cuts at: 4109 5839 4109
Size: 1730 4490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2452 3447 3575 844
Size: 1608 995 128 3489
Fragments arranged by size:
3489 1608 995 128
BsrBI CCG'CTC
Cuts at: 1394 1823 4107 1394
Size: 429 2284 3507
Fragments arranged by size:
3507 2284 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4715 4889 65
Size: 1183 3467 174 1396
Fragments arranged by size:
3467 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2072 2288 3009 4864 5051 1700
Size: 110 262 216 721 1855 187 2869
Fragments arranged by size:
2869 1855 721 262 216 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6220
BssHII G'CGCG_C
Cuts at: 1925 2020 2050 1925
Size: 95 30 6095
Fragments arranged by size:
6095 95 30
BssSI C'ACGA_G
Cuts at: 3445 4280 5292 3445
Size: 835 1012 4373
Fragments arranged by size:
4373 1012 835
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2095 794
Size: 1301 4919
BstBI TT'CG_AA
Cuts at: 5024 5024
Size: 6220
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6220
BstXI CCAn_nnnn'nTGG
Cuts at: 2710 3046 2710
Size: 336 5884
BstYI r'GATC_y
Cuts at: 1972 2352 2715 3426 3550 3917 4302 4319 5771
Size: 380 363 711 124 367 385 17 1452
Cuts at: 5771 5857 5869 6215 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 711 385 380 367 363 346
124 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2750 2750
Size: 6220
BtgI C'CryG_G
Cuts at: 513 1065 2004 2171 2205 2822 513
Size: 552 939 167 34 617 3911
Fragments arranged by size:
3911 939 617 552 167 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2098 3019 487
Size: 683 56 872 921 3688
Fragments arranged by size:
3688 921 872 683 56
BtsI GCAGTG_nn'
Cuts at: 3736 4530 4550 3736
Size: 794 20 5406
Fragments arranged by size:
5406 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6220
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6185
DraI TTT'AAA
Cuts at: 3524 3524
Size: 6220
DraIII CAC_nnn'GTG
Cuts at: 3130 3303 3130
Size: 173 6047
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5227 809
Size: 507 3911 1802
Fragments arranged by size:
3911 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2317 3011 3489
Size: 54 1425 50 275 18 487 694 478
Cuts at: 3489 3516 3667 4556 6185 8
Size: 27 151 889 1629 43
Fragments arranged by size:
1629 1425 889 694 487 478 275 151
54 50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2317 3667 1537
Size: 275 505 1350 4090
Fragments arranged by size:
4090 1350 505 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2669 2750 4150 1353
Size: 1316 81 1400 3423
Fragments arranged by size:
3423 1400 1316 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2126 4807 5181 5327 387
Size: 1064 138 537 2681 374 146 1280
Fragments arranged by size:
2681 1280 1064 537 374 146 138
EcoICRI GAG'CTC
Cuts at: 3094 3094
Size: 6220
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2237 2516 2573 2614 2732 3357
Size: 393 431 174 279 57 41 118 625
Cuts at: 3357 3438 4283 4880 5667 5935 1239
Size: 81 845 597 787 268 1524
Fragments arranged by size:
1524 845 787 625 597 431 393 279
268 174 118 81 57 41
EcoO109I rG'GnC_Cy
Cuts at: 2223 2386 2464 2550 3001 3059 2223
Size: 163 78 86 451 58 5384
Fragments arranged by size:
5384 451 163 86 78 58
EcoRI G'AATT_C
Cuts at: 3529 3907 3529
Size: 378 5842
FspI TGC'GCA
Cuts at: 2276 3110 4726 2276
Size: 834 1616 3770
Fragments arranged by size:
3770 1616 834
HaeII r_GCGC'y
Cuts at: 1678 2169 2205 2220 2246 2263 5367 1678
Size: 491 36 15 26 17 3104 2531
Fragments arranged by size:
3104 2531 491 36 26 17 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2420 2452 3428 3460 4861
Size: 32 135 32 848 32 976 32 1401
Cuts at: 4861 4893 4935 4967 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1401 976 848 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3564 3810 669
Size: 988 281 1626 246 3079
Fragments arranged by size:
3079 1626 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6220
HpaI GTT'AAC
Cuts at: 3810 3810
Size: 6220
KasI G'GCGC_C
Cuts at: 2201 2216 2259 2201
Size: 15 43 6162
Fragments arranged by size:
6162 43 15
KpnI G_GTAC'C
Cuts at: 3545 3545
Size: 6220
MfeI C'AATT_G
Cuts at: 3819 3819
Size: 6220
MluI A'CGCG_T
Cuts at: 3119 6096 3119
Size: 2977 3243
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5334 5518 1305
Size: 4029 184 2007
Fragments arranged by size:
4029 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4398
Fragments arranged by size:
4398 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4178 4537 4696 518
Size: 648 3012 359 159 2042
Fragments arranged by size:
3012 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2092 2173 2207 2233 2257 2372 2593 2824
Size: 486 81 34 26 24 115 221 231
Cuts at: 2824 2870 2914 3433 3636 4313 5461 5706 1606
Size: 46 44 519 203 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 519 486 245 231 221
203 115 81 46 44 34 26 24
NaeI GCC'GGC
Cuts at: 2074 3011 2074
Size: 937 5283
NarI GG'CG_CC
Cuts at: 2202 2217 2260 2202
Size: 15 43 6162
Fragments arranged by size:
6162 43 15
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4729
Fragments arranged by size:
4729 939 552
NdeI CA'TA_TG
Cuts at: 387 2665 387
Size: 2278 3942
NgoMIV G'CCGG_C
Cuts at: 2072 3009 2072
Size: 937 5283
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6220
NotI GC'GGCC_GC
Cuts at: 3667 3667
Size: 6220
NspI r_CATG'y
Cuts at: 1132 1229 2650 3578 5123 1132
Size: 97 1421 928 1545 2229
Fragments arranged by size:
2229 1545 1421 928 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6220
PciI A'CATG_T
Cuts at: 2646 5119 2646
Size: 2473 3747
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3193 1108
Size: 2085 4135
PfoI T'CCnGG_A
Cuts at: 3399 3399
Size: 6220
PmeI GTTT'AAAC
Cuts at: 3524 3524
Size: 6220
PmlI CAC'GTG
Cuts at: 3249 3249
Size: 6220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2955 2987 4289 4321 5802 5834 2955
Size: 32 1302 32 1481 32 3341
Fragments arranged by size:
3341 1481 1302 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2464 2550 2464
Size: 86 6134
PshAI GACnn'nnGTC
Cuts at: 1613 2151 2555 1613
Size: 538 404 5278
Fragments arranged by size:
5278 538 404
PsiI TTA'TAA
Cuts at: 3790 3790
Size: 6220
PspOMI G'GGCC_C
Cuts at: 2222 2222
Size: 6220
PstI C_TGCA'G
Cuts at: 830 2844 3269 3366 3572 830
Size: 2014 425 97 206 3478
Fragments arranged by size:
3478 2014 425 206 97
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6220
PvuII CAG'CTG
Cuts at: 2233 2372 2870 2914 3433 2233
Size: 139 498 44 519 5020
Fragments arranged by size:
5020 519 498 139 44
SacI G_AGCT'C
Cuts at: 3096 3096
Size: 6220
SacII CC_GC'GG
Cuts at: 2174 2208 2825 2174
Size: 34 617 5569
Fragments arranged by size:
5569 617 34
SalI G'TCGA_C
Cuts at: 1936 3562 1936
Size: 1626 4594
SbfI CC_TGCA'GG
Cuts at: 3572 3572
Size: 6220
ScaI AGT'ACT
Cuts at: 4468 6180 4468
Size: 1712 4508
SfcI C'TryA_G
Cuts at: 826 1045 2840 3237 3265 3362 3568 4703 5384
Size: 219 1795 397 28 97 206 1135 681
Cuts at: 5384 5575 826
Size: 191 1471
Fragments arranged by size:
1795 1471 1135 681 397 219 206 191
97 28
SfoI GGC'GCC
Cuts at: 2203 2218 2261 2203
Size: 15 43 6162
Fragments arranged by size:
6162 43 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
SgrAI Cr'CCGG_yG
Cuts at: 1700 2288 1700
Size: 588 5632
SmaI CCC'GGG
Cuts at: 1872 3547 1872
Size: 1675 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2468 4324 5225 5487 820
Size: 197 926 525 1856 901 262 1553
Fragments arranged by size:
1856 1553 926 901 525 262 197
SnaBI TAC'GTA
Cuts at: 493 4011 493
Size: 3518 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6220
SphI G_CATG'C
Cuts at: 1132 3578 1132
Size: 2446 3774
SspI AAT'ATT
Cuts at: 5 52 4144 5
Size: 47 4092 2081
Fragments arranged by size:
4092 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6220
StyI C'CwwG_G
Cuts at: 513 1065 2004 2757 3066 3659 513
Size: 552 939 753 309 593 3074
Fragments arranged by size:
3074 939 753 593 552 309
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2545 4247 4264 4417 4602 5047 325
Size: 1255 965 1702 17 153 185 445 1498
Fragments arranged by size:
1702 1498 1255 965 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4466 6178 96
Size: 274 80 33 51 1443 2489 1712 138
Fragments arranged by size:
2489 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6180 6212 6180
Size: 32 6188
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6220
XbaI T'CTAG_A
Cuts at: 3556 3556
Size: 6220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3194 3194
Size: 6220
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6220
XmaI C'CCGG_G
Cuts at: 1870 3545 1870
Size: 1675 4545
XmnI GAAnn'nnTTC
Cuts at: 4349 4349
Size: 6220
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2518 4028 276
Size: 53 83 186 936 984 1510 2468
Fragments arranged by size:
2468 1510 984 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BbvCI BfrBI BsiWI
BstZ17I EcoNI EcoRV FalI FseI FspAI NruI NsiI
PacI PspXI PsrI RsrII SanDI SapI SexAI SfiI
SrfI SwaI