Restriction map for
pFN21AB5915
(Circular) (Six-base) MAPSORT of: pFN21AB5915.seq Check: 8039 from: 1 to: 6220
pFN21AB5915 6220 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2407 2407
Size: 6220
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3141 3378 4030 278
Size: 53 83 186 936 1605 237 652 2468
Fragments arranged by size:
2468 1605 936 652 237 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3386 3563 1937
Size: 1449 177 4594
Fragments arranged by size:
4594 1449 177
Acc65I G'GTAC_C
Cuts at: 3541 3541
Size: 6220
AclI AA'CG_TT
Cuts at: 1563 2943 4347 4720 1563
Size: 1380 1404 373 3063
Fragments arranged by size:
3063 1404 1380 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2567 4283 5667 5935 1632
Size: 935 1716 1384 268 1917
Fragments arranged by size:
1917 1716 1384 935 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6023
AflIII A'CryG_T
Cuts at: 1203 3111 3131 3306 5119 6096 1203
Size: 1908 20 175 1813 977 1327
Fragments arranged by size:
1908 1813 1327 977 175 20
AgeI A'CCGG_T
Cuts at: 5051 5051
Size: 6220
AhdI GACnn_n'nnGTC
Cuts at: 4949 4949
Size: 6220
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6220
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6220
AlwNI CAG_nnn'CTG
Cuts at: 2164 2365 2557 3963 5535 2164
Size: 201 192 1406 1572 2849
Fragments arranged by size:
2849 1572 1406 201 192
ApaI G_GGCC'C
Cuts at: 2227 2249 3219 2227
Size: 22 970 5228
Fragments arranged by size:
5228 970 22
ApaLI G'TGCA_C
Cuts at: 4277 5433 4277
Size: 1156 5064
ApoI r'AATT_y
Cuts at: 1493 2977 3039 3529 3755 3907 6016 1493
Size: 1484 62 490 226 152 2109 1697
Fragments arranged by size:
2109 1697 1484 490 226 152 62
AseI AT'TA_AT
Cuts at: 160 1032 4774 160
Size: 872 3742 1606
Fragments arranged by size:
3742 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
AvaI C'yCGr_G
Cuts at: 1870 1943 2600 2960 3545 1870
Size: 73 657 360 585 4545
Fragments arranged by size:
4545 657 585 360 73
BamHI G'GATC_C
Cuts at: 3550 3917 3550
Size: 367 5853
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2986 3541 4996 618
Size: 325 217 627 1199 555 1455 1842
Fragments arranged by size:
1842 1455 1199 627 555 325 217
BanII G_rGCy'C
Cuts at: 1391 2227 2249 2652 3219 3539 1391
Size: 836 22 403 567 320 4072
Fragments arranged by size:
4072 836 567 403 320 22
BbeI G_GCGC'C
Cuts at: 2990 2990
Size: 6220
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5249
BbvCI CC'TCA_GC
Cuts at: 2174 2558 2174
Size: 384 5836
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2951 2985 4411 4445 1903
Size: 34 1014 34 1426 34 3678
Fragments arranged by size:
3678 1426 1014 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3050 4112 5322 3050
Size: 1062 1210 3948
Fragments arranged by size:
3948 1210 1062
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6220
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2222 4831
Size: 107 122 71 631 605 337 213 2609
Cuts at: 4831 136
Size: 1525
Fragments arranged by size:
2609 1525 631 605 337 213 122 107
71
BglII A'GATC_T
Cuts at: 2805 6215 2805
Size: 3410 2810
BlpI GC'TnA_GC
Cuts at: 3637 3637
Size: 6220
Bme1580I G_kGCm'C
Cuts at: 1790 2227 2249 3219 4281 5437 1790
Size: 437 22 970 1062 1156 2573
Fragments arranged by size:
2573 1156 1062 970 437 22
BmgBI CAC'GTC
Cuts at: 1318 2349 3309 1318
Size: 1031 960 4229
Fragments arranged by size:
4229 1031 960
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2491 2586 3951
Size: 416 53 83 399 24 1074 95 1365
Cuts at: 3951 4909 442
Size: 958 1753
Fragments arranged by size:
1753 1365 1074 958 416 399 95 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6220
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2347 3428 2347
Size: 1081 5139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2417 2514 2834 4880 1239
Size: 818 360 97 320 2046 2579
Fragments arranged by size:
2579 2046 818 360 320 97
Bpu10I CC'TnA_GC
Cuts at: 2129 2174 2558 2663 2129
Size: 45 384 105 5686
Fragments arranged by size:
5686 384 105 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3281 3398 4345 5210 5508 3281
Size: 117 947 865 298 3993
Fragments arranged by size:
3993 947 865 298 117
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2520 3063 3170 4883 882
Size: 617 1021 543 107 1713 2219
Fragments arranged by size:
2219 1713 1021 617 543 107
BsaAI yAC'GTr
Cuts at: 493 1204 4011 493
Size: 711 2807 2702
Fragments arranged by size:
2807 2702 711
BsaBI GATnn'nnATC
Cuts at: 6214 6214
Size: 6220
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2987 3138 3375 4027
Size: 53 83 186 936 1454 151 237 652
Cuts at: 4027 4409 5044 275
Size: 382 635 1451
Fragments arranged by size:
1454 1451 936 652 635 382 237 186
151 83 53
BsaWI w'CCGG_w
Cuts at: 3072 4653 5051 5325 5472 5883 3072
Size: 1581 398 274 147 411 3409
Fragments arranged by size:
3409 1581 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2566 2596 2639 2669 3293
Size: 30 165 30 1163 30 43 30 624
Cuts at: 3293 3323 1178
Size: 30 4075
Fragments arranged by size:
4075 1163 624 165 43 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2300 2508 2687 1186
Size: 844 270 208 179 4719
Fragments arranged by size:
4719 844 270 208 179
BseYI C'CCAG_C
Cuts at: 2831 3211 5423 5919 2831
Size: 380 2212 496 3132
Fragments arranged by size:
3132 2212 496 380
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2045 2177 3278 1163
Size: 573 309 132 1101 4105
Fragments arranged by size:
4105 1101 573 309 132
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 3670 4431 5459 1540
Size: 275 186 12 1657 761 1028 2301
Fragments arranged by size:
2301 1657 1028 761 275 186 12
BsiHKAI G_wGCw'C
Cuts at: 4281 4366 5437 4281
Size: 85 1071 5064
Fragments arranged by size:
5064 1071 85
BsmI GAATG_Cn'
Cuts at: 2818 3731 3824 2818
Size: 913 93 5214
Fragments arranged by size:
5214 913 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3366 3366
Size: 6220
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2227 2249 2652 3219 3539 4281 4366
Size: 399 437 22 403 567 320 742 85
Cuts at: 4366 5437 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 567 437 403 399 320
85 22
BspEI T'CCGG_A
Cuts at: 5883 5883
Size: 6220
BspHI T'CATG_A
Cuts at: 3363 4109 5839 3363
Size: 746 1730 3744
Fragments arranged by size:
3744 1730 746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2332 2407 2445 3575 844
Size: 1488 75 38 1130 3489
Fragments arranged by size:
3489 1488 1130 75 38
BsrBI CCG'CTC
Cuts at: 1394 1823 2109 4107 1394
Size: 429 286 1998 3507
Fragments arranged by size:
3507 1998 429 286
BsrDI GCAATG_nn'
Cuts at: 65 1248 4715 4889 65
Size: 1183 3467 174 1396
Fragments arranged by size:
3467 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2141 2216 4864 5051 1700
Size: 110 331 75 2648 187 2869
Fragments arranged by size:
2869 2648 331 187 110 75
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6220
BssHII G'CGCG_C
Cuts at: 1925 2178 1925
Size: 253 5967
BssSI C'ACGA_G
Cuts at: 2477 2591 3206 4280 5292 2477
Size: 114 615 1074 1012 3405
Fragments arranged by size:
3405 1074 1012 615 114
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2164 2365 794
Size: 1370 201 4649
Fragments arranged by size:
4649 1370 201
BstBI TT'CG_AA
Cuts at: 5024 5024
Size: 6220
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6220
BstXI CCAn_nnnn'nTGG
Cuts at: 3004 3004
Size: 6220
BstYI r'GATC_y
Cuts at: 1972 2028 2805 3550 3917 4302 4319 5771 5857
Size: 56 777 745 367 385 17 1452 86
Cuts at: 5857 5869 6215 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 777 745 385 367 346 86
56 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5668
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5481
Fragments arranged by size:
5481 683 56
BtsI GCAGTG_nn'
Cuts at: 3736 4530 4550 3736
Size: 794 20 5406
Fragments arranged by size:
5406 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6220
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6185
DraI TTT'AAA
Cuts at: 3524 3524
Size: 6220
DraIII CAC_nnn'GTG
Cuts at: 2482 3406 2482
Size: 924 5296
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5227 809
Size: 507 3911 1802
Fragments arranged by size:
3911 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2700 3667
Size: 54 1425 50 275 18 180 690 967
Cuts at: 3667 4556 6185 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 967 889 690 275 180 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2010 3667 1537
Size: 275 198 1657 4090
Fragments arranged by size:
4090 1657 275 198
EarI CTCTTCn'nnn_
Cuts at: 1353 2774 2867 3109 3168 4150 1353
Size: 1421 93 242 59 982 3423
Fragments arranged by size:
3423 1421 982 242 93 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2021 4807 5181 5327 387
Size: 1064 138 432 2786 374 146 1280
Fragments arranged by size:
2786 1280 1064 432 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2417 2514 2567 2834 4283 4880
Size: 393 425 360 97 53 267 1449 597
Cuts at: 4880 5667 5935 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1449 787 597 425 393 360 268
267 97 53
EcoNI CCTnn'n_nnAGG
Cuts at: 2132 2414 2441 2132
Size: 282 27 5911
Fragments arranged by size:
5911 282 27
EcoO109I rG'GnC_Cy
Cuts at: 2125 2223 2618 3215 2125
Size: 98 395 597 5130
Fragments arranged by size:
5130 597 395 98
EcoRI G'AATT_C
Cuts at: 3529 3907 3529
Size: 378 5842
FspI TGC'GCA
Cuts at: 2817 4726 2817
Size: 1909 4311
HaeII r_GCGC'y
Cuts at: 1678 2990 5367 1678
Size: 1312 2377 2531
Fragments arranged by size:
2531 2377 1312
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2566 2598 2639 2671 3293
Size: 32 135 32 994 32 41 32 622
Cuts at: 3293 3325 4861 4893 4935 4967 1373
Size: 32 1536 32 42 32 2626
Fragments arranged by size:
2626 1536 994 622 135 42 41 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3387 3564 3810 669
Size: 988 281 1449 177 246 3079
Fragments arranged by size:
3079 1449 988 281 246 177
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6220
HpaI GTT'AAC
Cuts at: 3810 3810
Size: 6220
KasI G'GCGC_C
Cuts at: 2986 2986
Size: 6220
KpnI G_GTAC'C
Cuts at: 3545 3545
Size: 6220
MfeI C'AATT_G
Cuts at: 3819 3819
Size: 6220
MluI A'CGCG_T
Cuts at: 6096 6096
Size: 6220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3132 5334 5518 1305
Size: 1827 2202 184 2007
Fragments arranged by size:
2202 2007 1827 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2702 10
Size: 54 1425 343 870 3528
Fragments arranged by size:
3528 1425 870 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4178 4537 4696 518
Size: 648 3012 359 159 2042
Fragments arranged by size:
3012 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2046 2164 2362 2698 2813 3636 4313 5461
Size: 440 118 198 336 115 823 677 1148
Cuts at: 5461 5706 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 823 677 440 336 245 198
118 115
NaeI GCC'GGC
Cuts at: 2143 2218 2143
Size: 75 6145
NarI GG'CG_CC
Cuts at: 2987 2987
Size: 6220
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5668
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6220
NgoMIV G'CCGG_C
Cuts at: 2141 2216 2141
Size: 75 6145
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6220
NotI GC'GGCC_GC
Cuts at: 3667 3667
Size: 6220
NspI r_CATG'y
Cuts at: 1132 1229 2119 3115 3135 3250 3578 5123 1132
Size: 97 890 996 20 115 328 1545 2229
Fragments arranged by size:
2229 1545 996 890 328 115 97 20
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6220
PciI A'CATG_T
Cuts at: 3111 3131 5119 3111
Size: 20 1988 4212
Fragments arranged by size:
4212 1988 20
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2509 2844 3211 3467 1108
Size: 1401 335 367 256 3861
Fragments arranged by size:
3861 1401 367 335 256
PfoI T'CCnGG_A
Cuts at: 2754 2754
Size: 6220
PmeI GTTT'AAAC
Cuts at: 3524 3524
Size: 6220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2675 2707 4289 4321 5802 5834 2675
Size: 32 1582 32 1481 32 3061
Fragments arranged by size:
3061 1582 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2618 2618
Size: 6220
PshAI GACnn'nnGTC
Cuts at: 1613 2585 1613
Size: 972 5248
PsiI TTA'TAA
Cuts at: 3790 3790
Size: 6220
PspOMI G'GGCC_C
Cuts at: 2223 2245 3215 2223
Size: 22 970 5228
Fragments arranged by size:
5228 970 22
PstI C_TGCA'G
Cuts at: 830 2343 2361 2442 3572 830
Size: 1513 18 81 1130 3478
Fragments arranged by size:
3478 1513 1130 81 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6220
PvuII CAG'CTG
Cuts at: 2164 2362 2164
Size: 198 6022
SalI G'TCGA_C
Cuts at: 1936 3385 3562 1936
Size: 1449 177 4594
Fragments arranged by size:
4594 1449 177
SanDI GG'GwC_CC
Cuts at: 2618 2618
Size: 6220
SbfI CC_TGCA'GG
Cuts at: 2343 2442 3572 2343
Size: 99 1130 4991
Fragments arranged by size:
4991 1130 99
ScaI AGT'ACT
Cuts at: 3095 4468 6180 3095
Size: 1373 1712 3135
Fragments arranged by size:
3135 1712 1373
SexAI A'CCwGG_T
Cuts at: 2721 2721
Size: 6220
SfcI C'TryA_G
Cuts at: 826 1045 2339 2357 2438 3568 4703 5384 5575
Size: 219 1294 18 81 1130 1135 681 191
Cuts at: 5575 826
Size: 1471
Fragments arranged by size:
1471 1294 1135 1130 681 219 191 81
18
SfoI GGC'GCC
Cuts at: 2988 2988
Size: 6220
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
SgrAI Cr'CCGG_yG
Cuts at: 1700 2216 1700
Size: 516 5704
SmaI CCC'GGG
Cuts at: 1872 3547 1872
Size: 1675 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3296 3413 4324 5225 5487 820
Size: 197 926 1353 117 911 901 262 1553
Fragments arranged by size:
1553 1353 926 911 901 262 197 117
SnaBI TAC'GTA
Cuts at: 493 4011 493
Size: 3518 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6220
SphI G_CATG'C
Cuts at: 1132 2119 3578 1132
Size: 987 1459 3774
Fragments arranged by size:
3774 1459 987
SspI AAT'ATT
Cuts at: 5 52 4144 5
Size: 47 4092 2081
Fragments arranged by size:
4092 2081 47
StuI AGG'CCT
Cuts at: 1517 2447 1517
Size: 930 5290
StyI C'CwwG_G
Cuts at: 513 1065 3659 513
Size: 552 2594 3074
Fragments arranged by size:
3074 2594 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4247 4264 4417 4602 5047 325
Size: 1255 2667 17 153 185 445 1498
Fragments arranged by size:
2667 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3093 3417 4466
Size: 274 80 33 51 1443 1116 324 1049
Cuts at: 4466 6178 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1116 1049 324 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6180 6212 6180
Size: 32 6188
Tth111I GACn'n_nGTC
Cuts at: 1316 2723 1316
Size: 1407 4813
XbaI T'CTAG_A
Cuts at: 3556 3556
Size: 6220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3187 3187
Size: 6220
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6220
XmaI C'CCGG_G
Cuts at: 1870 3545 1870
Size: 1675 4545
XmnI GAAnn'nnTTC
Cuts at: 3447 4349 3447
Size: 902 5318
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3139 3376 4028 276
Size: 53 83 186 936 1605 237 652 2468
Fragments arranged by size:
2468 1605 936 652 237 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SacII SapI
SfiI SrfI SwaI