Restriction map for
pFN21AB5913
(Circular) (Six-base) MAPSORT of: pFN21AB5913.seq Check: 4566 from: 1 to: 6217
pFN21AB5913 6217 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2754 2754
Size: 6217
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2544 4027 278
Size: 53 83 186 936 1008 1483 2468
Fragments arranged by size:
2468 1483 1008 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2454 3560 1937
Size: 517 1106 4594
Fragments arranged by size:
4594 1106 517
Acc65I G'GTAC_C
Cuts at: 3538 3538
Size: 6217
AclI AA'CG_TT
Cuts at: 1563 4344 4717 1563
Size: 2781 373 3063
Fragments arranged by size:
3063 2781 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3089 4280 5664 5932 1632
Size: 1457 1191 1384 268 1917
Fragments arranged by size:
1917 1457 1384 1191 268
AflII C'TTAA_G
Cuts at: 820 1017 3422 820
Size: 197 2405 3615
Fragments arranged by size:
3615 2405 197
AflIII A'CryG_T
Cuts at: 1203 2532 5116 6093 1203
Size: 1329 2584 977 1327
Fragments arranged by size:
2584 1329 1327 977
AgeI A'CCGG_T
Cuts at: 5048 5048
Size: 6217
AhdI GACnn_n'nnGTC
Cuts at: 4946 4946
Size: 6217
AleI CACnn'nnGTG
Cuts at: 1166 2769 1166
Size: 1603 4614
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6217
AlwNI CAG_nnn'CTG
Cuts at: 2130 3011 3067 3448 3960 5532 2130
Size: 881 56 381 512 1572 2815
Fragments arranged by size:
2815 1572 881 512 381 56
ApaI G_GGCC'C
Cuts at: 2984 3289 2984
Size: 305 5912
ApaLI G'TGCA_C
Cuts at: 4274 5430 4274
Size: 1156 5061
ApoI r'AATT_y
Cuts at: 1493 3526 3752 3904 6013 1493
Size: 2033 226 152 2109 1697
Fragments arranged by size:
2109 2033 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4771 160
Size: 872 3739 1606
Fragments arranged by size:
3739 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
AvaI C'yCGr_G
Cuts at: 1870 1943 3078 3186 3542 1870
Size: 73 1135 108 356 4545
Fragments arranged by size:
4545 1135 356 108 73
BamHI G'GATC_C
Cuts at: 2392 3547 3914 2392
Size: 1155 367 4695
Fragments arranged by size:
4695 1155 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2675 3538 4993 618
Size: 325 217 627 888 863 1455 1842
Fragments arranged by size:
1842 1455 888 863 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2083 2223 2871 2984 3187 3289 3536 1391
Size: 692 140 648 113 203 102 247 4072
Fragments arranged by size:
4072 692 648 247 203 140 113 102
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5246
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4408 4442 1903
Size: 34 50 34 2387 34 3678
Fragments arranged by size:
3678 2387 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3223 4109 5319 3223
Size: 886 1210 4121
Fragments arranged by size:
4121 1210 886
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6217
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2078 2447 4828
Size: 107 122 71 631 605 406 369 2381
Cuts at: 4828 136
Size: 1525
Fragments arranged by size:
2381 1525 631 605 406 369 122 107
71
BglII A'GATC_T
Cuts at: 6212 6212
Size: 6217
BlpI GC'TnA_GC
Cuts at: 2082 3192 3634 2082
Size: 1110 442 4665
Fragments arranged by size:
4665 1110 442
Bme1580I G_kGCm'C
Cuts at: 1790 2984 3289 4278 5434 1790
Size: 1194 305 989 1156 2573
Fragments arranged by size:
2573 1194 1156 989 305
BmgBI CAC'GTC
Cuts at: 1318 2562 1318
Size: 1244 4973
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2121 3948 4906
Size: 416 53 83 399 24 704 1827 958
Cuts at: 4906 442
Size: 1753
Fragments arranged by size:
1827 1753 958 704 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2237 2279 3264 3383 4877 1239
Size: 998 42 985 119 1494 2579
Fragments arranged by size:
2579 1494 998 985 119 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4342 5207 5505 4342
Size: 865 298 5054
Fragments arranged by size:
5054 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3240 4880 882
Size: 617 1741 1640 2219
Fragments arranged by size:
2219 1741 1640 617
BsaAI yAC'GTr
Cuts at: 493 1204 4008 493
Size: 711 2804 2702
Fragments arranged by size:
2804 2702 711
BsaBI GATnn'nnATC
Cuts at: 6211 6211
Size: 6217
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2541 4024 4406 5041
Size: 53 83 186 936 1008 1483 382 635
Cuts at: 5041 275
Size: 1451
Fragments arranged by size:
1483 1451 1008 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2481 2880 3327 4650 5048 5322 5469 5880 2481
Size: 399 447 1323 398 274 147 411 2818
Fragments arranged by size:
2818 1323 447 411 399 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2135 2165 2319 2349 2400
Size: 30 165 30 732 30 154 30 51
Cuts at: 2400 2430 1178
Size: 30 4965
Fragments arranged by size:
4965 732 165 154 51 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6217
BseYI C'CCAG_C
Cuts at: 2437 3289 5420 5916 2437
Size: 852 2131 496 2738
Fragments arranged by size:
2738 2131 852 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2382 3016 1163
Size: 573 646 634 4364
Fragments arranged by size:
4364 646 634 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2482 3667 4428 5456 1540
Size: 275 186 481 1185 761 1028 2301
Fragments arranged by size:
2301 1185 1028 761 481 275 186
BsiHKAI G_wGCw'C
Cuts at: 2223 3187 4278 4363 5434 2223
Size: 964 1091 85 1071 3006
Fragments arranged by size:
3006 1091 1071 964 85
BsmI GAATG_Cn'
Cuts at: 3079 3728 3821 3079
Size: 649 93 5475
Fragments arranged by size:
5475 649 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2223 2871 2984 3187 3289 3536
Size: 399 293 140 648 113 203 102 247
Cuts at: 3536 4278 4363 5434 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 648 399 293 247 203
140 113 102 85
BspEI T'CCGG_A
Cuts at: 3327 5880 3327
Size: 2553 3664
BspHI T'CATG_A
Cuts at: 4106 5836 4106
Size: 1730 4487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2508 2754 3052 3168 3572 844
Size: 1664 246 298 116 404 3489
Fragments arranged by size:
3489 1664 404 298 246 116
BsrBI CCG'CTC
Cuts at: 1394 1823 3196 4104 1394
Size: 429 1373 908 3507
Fragments arranged by size:
3507 1373 908 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2223 2997 4712 4886 65
Size: 1183 975 774 1715 174 1396
Fragments arranged by size:
1715 1396 1183 975 774 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4861 5048 1700
Size: 110 3051 187 2869
Fragments arranged by size:
3051 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6217
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6217
BssSI C'ACGA_G
Cuts at: 4277 5289 4277
Size: 1012 5205
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2639 794
Size: 1845 4372
BstBI TT'CG_AA
Cuts at: 5021 5021
Size: 6217
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6217
BstXI CCAn_nnnn'nTGG
Cuts at: 2771 3175 2771
Size: 404 5813
BstYI r'GATC_y
Cuts at: 1972 2392 2922 3547 3914 4299 4316 5768 5854
Size: 420 530 625 367 385 17 1452 86
Cuts at: 5854 5866 6212 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 625 530 420 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3458 3458
Size: 6217
BtgI C'CryG_G
Cuts at: 513 1065 2524 2657 3084 513
Size: 552 1459 133 427 3646
Fragments arranged by size:
3646 1459 552 427 133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5478
Fragments arranged by size:
5478 683 56
BtsI GCAGTG_nn'
Cuts at: 2210 3219 3733 4527 4547 2210
Size: 1009 514 794 20 3880
Fragments arranged by size:
3880 1009 794 514 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6182
DraI TTT'AAA
Cuts at: 3521 3521
Size: 6217
DraIII CAC_nnn'GTG
Cuts at: 3148 3148
Size: 6217
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5224 809
Size: 507 3908 1802
Fragments arranged by size:
3908 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 2448 2654
Size: 54 1425 50 275 18 240 378 206
Cuts at: 2654 2914 3450 3664 4553 6182 8
Size: 260 536 214 889 1629 43
Fragments arranged by size:
1629 1425 889 536 378 275 260 240
214 206 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3664 1537
Size: 275 1852 4090
Fragments arranged by size:
4090 1852 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2853 3374 4147 1353
Size: 1500 521 773 3423
Fragments arranged by size:
3423 1500 773 521
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4804 5178 5324 387
Size: 1064 138 3215 374 146 1280
Fragments arranged by size:
3215 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2221 3185 2221
Size: 964 5253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2279 3089 3264 3383 4280 4877
Size: 393 605 42 810 175 119 897 597
Cuts at: 4877 5664 5932 1239
Size: 787 268 1524
Fragments arranged by size:
1524 897 810 787 605 597 393 268
175 119 42
EcoNI CCTnn'n_nnAGG
Cuts at: 3023 3422 3023
Size: 399 5818
EcoO109I rG'GnC_Cy
Cuts at: 2263 2271 2433 2980 2981 3172 3285 3286 3486
Size: 8 162 547 1 191 113 1 200
Cuts at: 3486 2263
Size: 4994
Fragments arranged by size:
4994 547 200 191 162 113 8 1
1
EcoRI G'AATT_C
Cuts at: 3526 3904 3526
Size: 378 5839
EcoRV GAT'ATC
Cuts at: 2553 2553
Size: 6217
FspI TGC'GCA
Cuts at: 4723 4723
Size: 6217
HaeII r_GCGC'y
Cuts at: 1678 2368 2685 5364 1678
Size: 690 317 2679 2531
Fragments arranged by size:
2679 2531 690 317
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2167 2400 2432 3197
Size: 32 135 32 563 32 233 32 765
Cuts at: 3197 3229 3395 3427 4858 4890 4932 4964 1373
Size: 32 166 32 1431 32 42 32 2626
Fragments arranged by size:
2626 1431 765 563 233 166 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3361 3561 3807 669
Size: 988 281 1423 200 246 3079
Fragments arranged by size:
3079 1423 988 281 246 200
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6217
HpaI GTT'AAC
Cuts at: 3807 3807
Size: 6217
KpnI G_GTAC'C
Cuts at: 3542 3542
Size: 6217
MfeI C'AATT_G
Cuts at: 3816 3816
Size: 6217
MluI A'CGCG_T
Cuts at: 6093 6093
Size: 6217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2133 5331 5515 1305
Size: 828 3198 184 2007
Fragments arranged by size:
3198 2007 828 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2072 3452 10
Size: 54 1425 343 240 1380 2775
Fragments arranged by size:
2775 1425 1380 343 240 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2769 2991 3038 4175 4534 4693 518
Size: 648 1603 222 47 1137 359 159 2042
Fragments arranged by size:
2042 1603 1137 648 359 222 159 47
MspA1I CmG'CkG
Cuts at: 1606 2059 2629 3633 4310 5458 5703 1606
Size: 453 570 1004 677 1148 245 2120
Fragments arranged by size:
2120 1148 1004 677 570 453 245
NcoI C'CATG_G
Cuts at: 513 1065 3084 513
Size: 552 2019 3646
Fragments arranged by size:
3646 2019 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6217
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6217
NotI GC'GGCC_GC
Cuts at: 3664 3664
Size: 6217
NspI r_CATG'y
Cuts at: 1132 1229 2536 3518 3575 5120 1132
Size: 97 1307 982 57 1545 2229
Fragments arranged by size:
2229 1545 1307 982 97 57
PasI CC'CwG_GG
Cuts at: 1348 2266 1348
Size: 918 5299
PciI A'CATG_T
Cuts at: 2532 5116 2532
Size: 2584 3633
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6217
PfoI T'CCnGG_A
Cuts at: 3045 3241 3399 3045
Size: 196 158 5863
Fragments arranged by size:
5863 196 158
PmeI GTTT'AAAC
Cuts at: 3521 3521
Size: 6217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4286 4318 5799 5831 4286
Size: 32 1481 32 4672
Fragments arranged by size:
4672 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2271 2433 2271
Size: 162 6055
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6217
PsiI TTA'TAA
Cuts at: 3787 3787
Size: 6217
PspOMI G'GGCC_C
Cuts at: 2980 3285 2980
Size: 305 5912
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2273 2305 2273
Size: 32 6185
PstI C_TGCA'G
Cuts at: 830 2046 2187 2246 2976 3007 3063 3569 830
Size: 1216 141 59 730 31 56 506 3478
Fragments arranged by size:
3478 1216 730 506 141 59 56 31
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6217
PvuII CAG'CTG
Cuts at: 2059 2629 2059
Size: 570 5647
SacI G_AGCT'C
Cuts at: 2223 3187 2223
Size: 964 5253
SalI G'TCGA_C
Cuts at: 1936 3559 1936
Size: 1623 4594
SanDI GG'GwC_CC
Cuts at: 2433 2433
Size: 6217
SapI GCTCTTCn'nnn_
Cuts at: 3374 3374
Size: 6217
SbfI CC_TGCA'GG
Cuts at: 2976 3063 3569 2976
Size: 87 506 5624
Fragments arranged by size:
5624 506 87
ScaI AGT'ACT
Cuts at: 2291 3053 4465 6177 2291
Size: 762 1412 1712 2331
Fragments arranged by size:
2331 1712 1412 762
SexAI A'CCwGG_T
Cuts at: 3144 3144
Size: 6217
SfcI C'TryA_G
Cuts at: 826 1045 2042 2183 2242 2972 3003 3059 3565
Size: 219 997 141 59 730 31 56 506
Cuts at: 3565 4700 5381 5572 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 997 730 681 506 219 191
141 59 56 31
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6217
SmaI CCC'GGG
Cuts at: 1872 3544 1872
Size: 1672 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3422 4321 5222 5484 820
Size: 197 926 1479 899 901 262 1553
Fragments arranged by size:
1553 1479 926 901 899 262 197
SnaBI TAC'GTA
Cuts at: 493 4008 493
Size: 3515 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6217
SphI G_CATG'C
Cuts at: 1132 3575 1132
Size: 2443 3774
SspI AAT'ATT
Cuts at: 5 52 4141 5
Size: 47 4089 2081
Fragments arranged by size:
4089 2081 47
StuI AGG'CCT
Cuts at: 1517 3029 1517
Size: 1512 4705
StyI C'CwwG_G
Cuts at: 513 1065 2373 2787 3084 3168 3656 513
Size: 552 1308 414 297 84 488 3074
Fragments arranged by size:
3074 1308 552 488 414 297 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2537 2958 3264 4244 4261 4414 4599
Size: 1255 957 421 306 980 17 153 185
Cuts at: 4599 5044 325
Size: 445 1498
Fragments arranged by size:
1498 1255 980 957 445 421 306 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2010 2289 3051
Size: 274 80 33 51 1443 33 279 762
Cuts at: 3051 4463 6175 96
Size: 1412 1712 138
Fragments arranged by size:
1712 1443 1412 762 279 274 138 80
51 33 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2189 2221 2319 2351 6177 6209 2189
Size: 32 98 32 3826 32 2197
Fragments arranged by size:
3826 2197 98 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2822 1316
Size: 1506 4711
XbaI T'CTAG_A
Cuts at: 3553 3553
Size: 6217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2108 2108
Size: 6217
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6217
XmaI C'CCGG_G
Cuts at: 1870 3542 1870
Size: 1672 4545
XmnI GAAnn'nnTTC
Cuts at: 4346 4346
Size: 6217
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2542 4025 276
Size: 53 83 186 936 1008 1483 2468
Fragments arranged by size:
2468 1483 1008 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI RsrII SacII SfiI SfoI SrfI SwaI