Restriction map for
pFN21AB5911
(Circular) (Six-base) MAPSORT of: pFN21AB5911.seq Check: 1869 from: 1 to: 6217
pFN21AB5911 6217 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2910 3177 3181 2910
Size: 267 4 5946
Fragments arranged by size:
5946 267 4
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4027 278
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2064 3560 1937
Size: 127 1496 4594
Fragments arranged by size:
4594 1496 127
Acc65I G'GTAC_C
Cuts at: 3538 3538
Size: 6217
AclI AA'CG_TT
Cuts at: 1563 4344 4717 1563
Size: 2781 373 3063
Fragments arranged by size:
3063 2781 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2321 4280 5664 5932 1632
Size: 689 1959 1384 268 1917
Fragments arranged by size:
1959 1917 1384 689 268
AfeI AGC'GCT
Cuts at: 3037 3037
Size: 6217
AflII C'TTAA_G
Cuts at: 820 1017 2537 820
Size: 197 1520 4500
Fragments arranged by size:
4500 1520 197
AflIII A'CryG_T
Cuts at: 1203 5116 6093 1203
Size: 3913 977 1327
Fragments arranged by size:
3913 1327 977
AgeI A'CCGG_T
Cuts at: 5048 5048
Size: 6217
AhdI GACnn_n'nnGTC
Cuts at: 4946 4946
Size: 6217
AleI CACnn'nnGTG
Cuts at: 1166 3356 1166
Size: 2190 4027
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2312 3185 1184
Size: 1128 873 4216
Fragments arranged by size:
4216 1128 873
AlwNI CAG_nnn'CTG
Cuts at: 3123 3133 3217 3334 3960 5532 3123
Size: 10 84 117 626 1572 3808
Fragments arranged by size:
3808 1572 626 117 84 10
ApaI G_GGCC'C
Cuts at: 2252 3508 2252
Size: 1256 4961
ApaLI G'TGCA_C
Cuts at: 4274 5430 4274
Size: 1156 5061
ApoI r'AATT_y
Cuts at: 1493 3526 3752 3904 6013 1493
Size: 2033 226 152 2109 1697
Fragments arranged by size:
2109 2033 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4771 160
Size: 872 3739 1606
Fragments arranged by size:
3739 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
AvaI C'yCGr_G
Cuts at: 1870 1943 2566 3083 3482 3542 1870
Size: 73 623 517 399 60 4545
Fragments arranged by size:
4545 623 517 399 73 60
BamHI G'GATC_C
Cuts at: 3547 3914 3547
Size: 367 5850
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2138 2201 2340 3010 3165
Size: 325 217 627 351 63 139 670 155
Cuts at: 3165 3538 4993 618
Size: 373 1455 1842
Fragments arranged by size:
1842 1455 670 627 373 351 325 217
155 139 63
BanII G_rGCy'C
Cuts at: 1391 2252 2418 2466 2697 2949 3508 3536 1391
Size: 861 166 48 231 252 559 28 4072
Fragments arranged by size:
4072 861 559 252 231 166 48 28
BbeI G_GCGC'C
Cuts at: 2142 2344 2142
Size: 202 6015
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2027 928
Size: 971 128 5118
Fragments arranged by size:
5118 971 128
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2697 2731 4408 4442 1903
Size: 34 760 34 1677 34 3678
Fragments arranged by size:
3678 1677 760 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2720 4109 5319 2720
Size: 1389 1210 3618
Fragments arranged by size:
3618 1389 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6217
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2814 2911 3178
Size: 107 122 71 631 605 1142 97 267
Cuts at: 3178 3404 4828 136
Size: 226 1424 1525
Fragments arranged by size:
1525 1424 1142 631 605 267 226 122
107 97 71
BglII A'GATC_T
Cuts at: 6212 6212
Size: 6217
BlpI GC'TnA_GC
Cuts at: 3467 3634 3467
Size: 167 6050
Bme1580I G_kGCm'C
Cuts at: 1790 2252 3015 3508 4278 5434 1790
Size: 462 763 493 770 1156 2573
Fragments arranged by size:
2573 1156 770 763 493 462
BmgBI CAC'GTC
Cuts at: 1318 2025 1318
Size: 707 5510
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3948 4906 442
Size: 416 53 83 399 24 2531 958 1753
Fragments arranged by size:
2531 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6217
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2881 2881
Size: 6217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2465 2915 2951 3050 3083 3160 4877 1239
Size: 1226 450 36 99 33 77 1717 2579
Fragments arranged by size:
2579 1717 1226 450 99 77 36 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4342 5207 5505 4342
Size: 865 298 5054
Fragments arranged by size:
5054 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4880 882
Size: 617 3381 2219
Fragments arranged by size:
3381 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2497 4008 493
Size: 711 1293 1511 2702
Fragments arranged by size:
2702 1511 1293 711
BsaBI GATnn'nnATC
Cuts at: 6211 6211
Size: 6217
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2061 2139 2341 4024
Size: 53 83 186 936 528 78 202 1683
Cuts at: 4024 4406 5041 275
Size: 382 635 1451
Fragments arranged by size:
1683 1451 936 635 528 382 202 186
83 78 53
BsaWI w'CCGG_w
Cuts at: 3093 4650 5048 5322 5469 5880 3093
Size: 1557 398 274 147 411 3430
Fragments arranged by size:
3430 1557 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2387 2417 2654 2684 3158
Size: 30 165 30 984 30 237 30 474
Cuts at: 3158 3188 3463 3493 1178
Size: 30 275 30 3902
Fragments arranged by size:
3902 984 474 275 237 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3329 1186
Size: 2143 4074
BseYI C'CCAG_C
Cuts at: 3157 3289 3424 5420 5916 3157
Size: 132 135 1996 496 3458
Fragments arranged by size:
3458 1996 496 135 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2240 3334 1163
Size: 573 504 1094 4046
Fragments arranged by size:
4046 1094 573 504
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2148 3667 4428 5456 1540
Size: 275 186 147 1519 761 1028 2301
Fragments arranged by size:
2301 1519 1028 761 275 186 147
BsiHKAI G_wGCw'C
Cuts at: 2100 2418 2901 3473 4278 4363 5434 2100
Size: 318 483 572 805 85 1071 2883
Fragments arranged by size:
2883 1071 805 572 483 318 85
BsmI GAATG_Cn'
Cuts at: 3728 3821 3728
Size: 93 6124
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2252 2418 2466 2697 2901 2949
Size: 399 310 152 166 48 231 204 48
Cuts at: 2949 3015 3473 3508 3536 4278 4363 5434 1391
Size: 66 458 35 28 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 458 399 310 231 204
166 152 85 66 48 48 35 28
BspEI T'CCGG_A
Cuts at: 5880 5880
Size: 6217
BspHI T'CATG_A
Cuts at: 4106 5836 4106
Size: 1730 4487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2529 2658 2910 2964 3177 3181 3572 844
Size: 1685 129 252 54 213 4 391 3489
Fragments arranged by size:
3489 1685 391 252 213 129 54 4
BsrBI CCG'CTC
Cuts at: 1394 1823 4104 1394
Size: 429 2281 3507
Fragments arranged by size:
3507 2281 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4712 4886 65
Size: 1183 3464 174 1396
Fragments arranged by size:
3464 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2772 4861 5048 1700
Size: 110 962 2089 187 2869
Fragments arranged by size:
2869 2089 962 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6217
BssHII G'CGCG_C
Cuts at: 1925 3142 3388 1925
Size: 1217 246 4754
Fragments arranged by size:
4754 1217 246
BssSI C'ACGA_G
Cuts at: 2612 4277 5289 2612
Size: 1665 1012 3540
Fragments arranged by size:
3540 1665 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3123 794
Size: 2329 3888
BstBI TT'CG_AA
Cuts at: 5021 5021
Size: 6217
BstEII G'GTnAC_C
Cuts at: 1184 2173 2623 1184
Size: 989 450 4778
Fragments arranged by size:
4778 989 450
BstXI CCAn_nnnn'nTGG
Cuts at: 2170 2170
Size: 6217
BstYI r'GATC_y
Cuts at: 1972 3547 3914 4299 4316 5768 5854 5866 6212
Size: 1575 367 385 17 1452 86 12 346
Cuts at: 6212 1972
Size: 1977
Fragments arranged by size:
1977 1575 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2163 2812 2949 2997 3417 513
Size: 552 1098 649 137 48 420 3313
Fragments arranged by size:
3313 1098 649 552 420 137 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5478
Fragments arranged by size:
5478 683 56
BtsI GCAGTG_nn'
Cuts at: 2844 3257 3733 4527 4547 2844
Size: 413 476 794 20 4514
Fragments arranged by size:
4514 794 476 413 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6182
DraI TTT'AAA
Cuts at: 3521 3521
Size: 6217
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2365 5224 809
Size: 507 1049 2859 1802
Fragments arranged by size:
2859 1802 1049 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 2145 2160
Size: 54 1425 50 275 18 285 30 15
Cuts at: 2160 2994 3000 3303 3447 3664 4553 6182 8
Size: 834 6 303 144 217 889 1629 43
Fragments arranged by size:
1629 1425 889 834 303 285 275 217
144 54 50 43 30 18 15 6
EagI C'GGCC_G
Cuts at: 1537 1812 2145 3664 1537
Size: 275 333 1519 4090
Fragments arranged by size:
4090 1519 333 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2687 3350 4147 1353
Size: 1334 663 797 3423
Fragments arranged by size:
3423 1334 797 663
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4804 5178 5324 387
Size: 1064 138 3215 374 146 1280
Fragments arranged by size:
3215 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2416 2416
Size: 6217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2321 2465 2915 2951 3050 3083 3160
Size: 393 689 144 450 36 99 33 77
Cuts at: 3160 4280 4877 5664 5932 1239
Size: 1120 597 787 268 1524
Fragments arranged by size:
1524 1120 787 689 597 450 393 268
144 99 77 36 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2192 2300 2672 2930 2192
Size: 108 372 258 5479
Fragments arranged by size:
5479 372 258 108
EcoO109I rG'GnC_Cy
Cuts at: 2249 2305 2677 2923 3504 3505 2249
Size: 56 372 246 581 1 4961
Fragments arranged by size:
4961 581 372 246 56 1
EcoRI G'AATT_C
Cuts at: 3526 3904 3526
Size: 378 5839
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 6217
FspI TGC'GCA
Cuts at: 2654 3275 3383 4723 2654
Size: 621 108 1340 4148
Fragments arranged by size:
4148 1340 621 108
HaeII r_GCGC'y
Cuts at: 1678 2142 2156 2171 2344 2850 3039 3328 5364
Size: 464 14 15 173 506 189 289 2036
Cuts at: 5364 1678
Size: 2531
Fragments arranged by size:
2531 2036 506 464 289 189 173 15
14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3413 3445 4858 4890 4932
Size: 32 135 32 1841 32 1413 32 42
Cuts at: 4932 4964 1373
Size: 32 2626
Fragments arranged by size:
2626 1841 1413 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3561 3807 669
Size: 988 281 1623 246 3079
Fragments arranged by size:
3079 1623 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6217
HpaI GTT'AAC
Cuts at: 3807 3807
Size: 6217
KasI G'GCGC_C
Cuts at: 2138 2340 2138
Size: 202 6015
KpnI G_GTAC'C
Cuts at: 3542 3542
Size: 6217
MfeI C'AATT_G
Cuts at: 3816 3816
Size: 6217
MluI A'CGCG_T
Cuts at: 6093 6093
Size: 6217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2997 5331 5515 1305
Size: 1692 2334 184 2007
Fragments arranged by size:
2334 2007 1692 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2117 2996 3305 10
Size: 54 1425 343 285 879 309 2922
Fragments arranged by size:
2922 1425 879 343 309 285 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2328 2334 2450 2552 3002 3008 3356
Size: 648 1162 6 116 102 450 6 348
Cuts at: 3356 4175 4534 4693 518
Size: 819 359 159 2042
Fragments arranged by size:
2042 1162 819 648 450 359 348 159
116 102 6 6
MspA1I CmG'CkG
Cuts at: 1606 2439 2755 2872 3028 3232 3331 3419 3445
Size: 833 316 117 156 204 99 88 26
Cuts at: 3445 3633 4310 5458 5703 1606
Size: 188 677 1148 245 2120
Fragments arranged by size:
2120 1148 833 677 316 245 204 188
156 117 99 88 26
NarI GG'CG_CC
Cuts at: 2139 2341 2139
Size: 202 6015
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5665
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6217
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6217
NotI GC'GGCC_GC
Cuts at: 3664 3664
Size: 6217
NspI r_CATG'y
Cuts at: 1132 1229 2333 2980 3388 3575 5120 1132
Size: 97 1104 647 408 187 1545 2229
Fragments arranged by size:
2229 1545 1104 647 408 187 97
PasI CC'CwG_GG
Cuts at: 1348 3508 1348
Size: 2160 4057
PciI A'CATG_T
Cuts at: 5116 5116
Size: 6217
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2839 3133 1108
Size: 1731 294 4192
Fragments arranged by size:
4192 1731 294
PfoI T'CCnGG_A
Cuts at: 2892 2892
Size: 6217
PmeI GTTT'AAAC
Cuts at: 3521 3521
Size: 6217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4286 4318 5799 5831 4286
Size: 32 1481 32 4672
Fragments arranged by size:
4672 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2305 2305
Size: 6217
PshAI GACnn'nnGTC
Cuts at: 1613 2583 1613
Size: 970 5247
PsiI TTA'TAA
Cuts at: 3787 3787
Size: 6217
PspOMI G'GGCC_C
Cuts at: 2248 3504 2248
Size: 1256 4961
PstI C_TGCA'G
Cuts at: 830 2438 2754 3569 830
Size: 1608 316 815 3478
Fragments arranged by size:
3478 1608 815 316
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6217
PvuII CAG'CTG
Cuts at: 2872 3331 3445 2872
Size: 459 114 5644
Fragments arranged by size:
5644 459 114
SacI G_AGCT'C
Cuts at: 2418 2418
Size: 6217
SacII CC_GC'GG
Cuts at: 3420 3420
Size: 6217
SalI G'TCGA_C
Cuts at: 1936 3559 1936
Size: 1623 4594
SbfI CC_TGCA'GG
Cuts at: 3569 3569
Size: 6217
ScaI AGT'ACT
Cuts at: 4465 6177 4465
Size: 1712 4505
SexAI A'CCwGG_T
Cuts at: 2271 3132 2271
Size: 861 5356
SfcI C'TryA_G
Cuts at: 826 1045 2434 2750 3247 3565 4700 5381 5572
Size: 219 1389 316 497 318 1135 681 191
Cuts at: 5572 826
Size: 1471
Fragments arranged by size:
1471 1389 1135 681 497 318 316 219
191
SfoI GGC'GCC
Cuts at: 2140 2342 2140
Size: 202 6015
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6217
SmaI CCC'GGG
Cuts at: 1872 3544 1872
Size: 1672 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2537 4321 5222 5484 820
Size: 197 926 594 1784 901 262 1553
Fragments arranged by size:
1784 1553 926 901 594 262 197
SnaBI TAC'GTA
Cuts at: 493 4008 493
Size: 3515 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6217
SphI G_CATG'C
Cuts at: 1132 2333 3388 3575 1132
Size: 1201 1055 187 3774
Fragments arranged by size:
3774 1201 1055 187
SspI AAT'ATT
Cuts at: 5 52 4141 5
Size: 47 4089 2081
Fragments arranged by size:
4089 2081 47
StuI AGG'CCT
Cuts at: 1517 2936 1517
Size: 1419 4798
StyI C'CwwG_G
Cuts at: 513 1065 2406 2673 3656 513
Size: 552 1341 267 983 3074
Fragments arranged by size:
3074 1341 983 552 267
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2180 4244 4261 4414 4599 5044 325
Size: 1255 600 2064 17 153 185 445 1498
Fragments arranged by size:
2064 1498 1255 600 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2454 4463 6175
Size: 274 80 33 51 1443 477 2009 1712
Cuts at: 6175 96
Size: 138
Fragments arranged by size:
2009 1712 1443 477 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2387 2419 3080 3112 3158 3190 3463 3495 6177
Size: 32 661 32 46 32 273 32 2682
Cuts at: 6177 6209 2387
Size: 32 2395
Fragments arranged by size:
2682 2395 661 273 46 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6217
XbaI T'CTAG_A
Cuts at: 3553 3553
Size: 6217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2341 2785 3004 3058 3154 2341
Size: 444 219 54 96 5404
Fragments arranged by size:
5404 444 219 96 54
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6217
XmaI C'CCGG_G
Cuts at: 1870 3542 1870
Size: 1672 4545
XmnI GAAnn'nnTTC
Cuts at: 2240 4346 2240
Size: 2106 4111
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4025 276
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SanDI SapI SfiI SrfI SwaI