Restriction map for
pFN21AB5909
(Circular) (Six-base) MAPSORT of: pFN21AB5909.seq Check: 5400 from: 1 to: 6217
pFN21AB5909 6217 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2620 2620
Size: 6217
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4027 278
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3560 1937
Size: 1623 4594
Acc65I G'GTAC_C
Cuts at: 2117 3344 3538 2117
Size: 1227 194 4796
Fragments arranged by size:
4796 1227 194
AclI AA'CG_TT
Cuts at: 1563 4344 4717 1563
Size: 2781 373 3063
Fragments arranged by size:
3063 2781 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4280 5664 5932 1632
Size: 2648 1384 268 1917
Fragments arranged by size:
2648 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 3275 820
Size: 197 2258 3762
Fragments arranged by size:
3762 2258 197
AflIII A'CryG_T
Cuts at: 1203 5116 6093 1203
Size: 3913 977 1327
Fragments arranged by size:
3913 1327 977
AgeI A'CCGG_T
Cuts at: 5048 5048
Size: 6217
AhdI GACnn_n'nnGTC
Cuts at: 4946 4946
Size: 6217
AleI CACnn'nnGTG
Cuts at: 1166 2049 2810 3032 3443 1166
Size: 883 761 222 411 3940
Fragments arranged by size:
3940 883 761 411 222
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6217
AlwNI CAG_nnn'CTG
Cuts at: 2290 2836 3236 3960 5532 2290
Size: 546 400 724 1572 2975
Fragments arranged by size:
2975 1572 724 546 400
ApaI G_GGCC'C
Cuts at: 2917 3185 2917
Size: 268 5949
ApaLI G'TGCA_C
Cuts at: 3069 4274 5430 3069
Size: 1205 1156 3856
Fragments arranged by size:
3856 1205 1156
ApoI r'AATT_y
Cuts at: 1493 3526 3752 3904 6013 1493
Size: 2033 226 152 2109 1697
Fragments arranged by size:
2109 2033 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4771 160
Size: 872 3739 1606
Fragments arranged by size:
3739 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
AvaI C'yCGr_G
Cuts at: 1870 1943 2385 3178 3542 1870
Size: 73 442 793 364 4545
Fragments arranged by size:
4545 793 442 364 73
BamHI G'GATC_C
Cuts at: 2673 3547 3914 2673
Size: 874 367 4976
Fragments arranged by size:
4976 874 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2117 3344 3538 4993 618
Size: 325 217 627 330 1227 194 1455 1842
Fragments arranged by size:
1842 1455 1227 627 330 325 217 194
BanII G_rGCy'C
Cuts at: 1391 2616 2917 2975 3185 3536 1391
Size: 1225 301 58 210 351 4072
Fragments arranged by size:
4072 1225 351 301 210 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2585 928
Size: 971 686 4560
Fragments arranged by size:
4560 971 686
BbvCI CC'TCA_GC
Cuts at: 2203 2939 2203
Size: 736 5481
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4408 4442 1903
Size: 34 2471 34 3678
Fragments arranged by size:
3678 2471 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2065 4109 5319 2065
Size: 2044 1210 2963
Fragments arranged by size:
2963 2044 1210
BclI T'GATC_A
Cuts at: 1548 2983 1548
Size: 1435 4782
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3180 4828 136
Size: 107 122 71 631 605 1508 1648 1525
Fragments arranged by size:
1648 1525 1508 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3129 3378 6212 3129
Size: 249 2834 3134
Fragments arranged by size:
3134 2834 249
BlpI GC'TnA_GC
Cuts at: 2615 3634 2615
Size: 1019 5198
Bme1580I G_kGCm'C
Cuts at: 1790 2433 2917 3073 3185 3368 4278 5434 1790
Size: 643 484 156 112 183 910 1156 2573
Fragments arranged by size:
2573 1156 910 643 484 183 156 112
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6217
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3001 3948 4906
Size: 416 53 83 399 24 1584 947 958
Cuts at: 4906 442
Size: 1753
Fragments arranged by size:
1753 1584 958 947 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4877 1239
Size: 3638 2579
Bpu10I CC'TnA_GC
Cuts at: 2203 2939 2969 2203
Size: 736 30 5451
Fragments arranged by size:
5451 736 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3485 4342 5207 5505 3485
Size: 857 865 298 4197
Fragments arranged by size:
4197 865 857 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3038 4880 882
Size: 617 1539 1842 2219
Fragments arranged by size:
2219 1842 1539 617
BsaAI yAC'GTr
Cuts at: 493 1204 2644 4008 493
Size: 711 1440 1364 2702
Fragments arranged by size:
2702 1440 1364 711
BsaBI GATnn'nnATC
Cuts at: 3128 3383 6211 3128
Size: 255 2828 3134
Fragments arranged by size:
3134 2828 255
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4024 4406 5041 275
Size: 53 83 186 936 2491 382 635 1451
Fragments arranged by size:
2491 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4650 5048 5322 5469 5880 4650
Size: 398 274 147 411 4987
Fragments arranged by size:
4987 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5992
Fragments arranged by size:
5992 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2165 2606 2965 3235 1186
Size: 979 441 359 270 4168
Fragments arranged by size:
4168 979 441 359 270
BseYI C'CCAG_C
Cuts at: 5420 5916 5420
Size: 496 5721
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2249 1163
Size: 573 513 5131
Fragments arranged by size:
5131 573 513
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3667 4428 5456 1540
Size: 275 186 1666 761 1028 2301
Fragments arranged by size:
2301 1666 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2046 2616 2975 3027 3073 4278 4363 5434 2046
Size: 570 359 52 46 1205 85 1071 2829
Fragments arranged by size:
2829 1205 1071 570 359 85 52 46
BsmI GAATG_Cn'
Cuts at: 2664 3728 3821 2664
Size: 1064 93 5060
Fragments arranged by size:
5060 1064 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2366 2366
Size: 6217
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2433 2616 2917 2975 3027 3073
Size: 399 256 387 183 301 58 52 46
Cuts at: 3073 3185 3368 3536 4278 4363 5434 1391
Size: 112 183 168 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 387 301 256 183
183 168 112 85 58 52 46
BspEI T'CCGG_A
Cuts at: 5880 5880
Size: 6217
BspHI T'CATG_A
Cuts at: 4106 5836 4106
Size: 1730 4487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2620 3572 844
Size: 1776 952 3489
Fragments arranged by size:
3489 1776 952
BsrBI CCG'CTC
Cuts at: 1394 1823 2083 4104 1394
Size: 429 260 2021 3507
Fragments arranged by size:
3507 2021 429 260
BsrDI GCAATG_nn'
Cuts at: 65 1248 2103 2352 4712 4886 65
Size: 1183 855 249 2360 174 1396
Fragments arranged by size:
2360 1396 1183 855 249 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2625 4861 5048 1700
Size: 110 815 2236 187 2869
Fragments arranged by size:
2869 2236 815 187 110
BsrGI T'GTAC_A
Cuts at: 96 2899 96
Size: 2803 3414
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6217
BssSI C'ACGA_G
Cuts at: 4277 5289 4277
Size: 1012 5205
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6217
BstBI TT'CG_AA
Cuts at: 5021 5021
Size: 6217
BstEII G'GTnAC_C
Cuts at: 1184 2803 1184
Size: 1619 4598
BstXI CCAn_nnnn'nTGG
Cuts at: 3445 3445
Size: 6217
BstYI r'GATC_y
Cuts at: 1972 2673 2712 3129 3262 3378 3547 3914 4299
Size: 701 39 417 133 116 169 367 385
Cuts at: 4299 4316 5768 5854 5866 6212 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 701 417 385 367 346 169
133 116 86 39 17 12
Bsu36I CC'TnA_GG
Cuts at: 2589 2589
Size: 6217
BtgI C'CryG_G
Cuts at: 513 1065 2556 3111 513
Size: 552 1491 555 3619
Fragments arranged by size:
3619 1491 555 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3181 3457 487
Size: 683 56 1955 276 3247
Fragments arranged by size:
3247 1955 683 276 56
BtsI GCAGTG_nn'
Cuts at: 2376 3733 4527 4547 2376
Size: 1357 794 20 4046
Fragments arranged by size:
4046 1357 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2445 2480 529
Size: 35 1881 35 4266
Fragments arranged by size:
4266 1881 35 35
DraI TTT'AAA
Cuts at: 3521 3521
Size: 6217
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3248 5224 809
Size: 507 1932 1976 1802
Fragments arranged by size:
1976 1932 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2220 3159 3172
Size: 54 1425 50 275 18 390 939 13
Cuts at: 3172 3664 4553 6182 8
Size: 492 889 1629 43
Fragments arranged by size:
1629 1425 939 889 492 390 275 54
50 43 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 3664 1537
Size: 275 1852 4090
Fragments arranged by size:
4090 1852 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2564 2604 4147 1353
Size: 1211 40 1543 3423
Fragments arranged by size:
3423 1543 1211 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2744 4804 5178 5324 387
Size: 1064 138 1155 2060 374 146 1280
Fragments arranged by size:
2060 1280 1155 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2614 2973 2614
Size: 359 5858
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4280 4877 5664 5932 1239
Size: 393 2648 597 787 268 1524
Fragments arranged by size:
2648 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2833 3182 2833
Size: 349 5868
EcoRI G'AATT_C
Cuts at: 3526 3904 3526
Size: 378 5839
FspI TGC'GCA
Cuts at: 3269 4723 3269
Size: 1454 4763
HaeII r_GCGC'y
Cuts at: 1678 5364 1678
Size: 3686 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3032 3064 3230 3262 3305
Size: 32 135 32 1460 32 166 32 43
Cuts at: 3305 3337 4858 4890 4932 4964 1373
Size: 32 1521 32 42 32 2626
Fragments arranged by size:
2626 1521 1460 166 135 43 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3561 3807 669
Size: 988 281 1623 246 3079
Fragments arranged by size:
3079 1623 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2232 2528 3272 748
Size: 1484 296 744 3693
Fragments arranged by size:
3693 1484 744 296
HpaI GTT'AAC
Cuts at: 3807 3807
Size: 6217
KpnI G_GTAC'C
Cuts at: 2121 3348 3542 2121
Size: 1227 194 4796
Fragments arranged by size:
4796 1227 194
MfeI C'AATT_G
Cuts at: 3816 3816
Size: 6217
MluI A'CGCG_T
Cuts at: 6093 6093
Size: 6217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2724 5331 5515 1305
Size: 1419 2607 184 2007
Fragments arranged by size:
2607 2007 1419 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3161 3174 10
Size: 54 1425 343 1329 13 3053
Fragments arranged by size:
3053 1425 1329 343 54 13
MslI CAynn'nnrTG
Cuts at: 518 1166 2049 2076 2810 2922 3032 3443 4175
Size: 648 883 27 734 112 110 411 732
Cuts at: 4175 4534 4693 518
Size: 359 159 2042
Fragments arranged by size:
2042 883 734 732 648 411 359 159
112 110 27
MspA1I CmG'CkG
Cuts at: 1606 2290 3233 3298 3633 4310 5458 5703 1606
Size: 684 943 65 335 677 1148 245 2120
Fragments arranged by size:
2120 1148 943 684 677 335 245 65
NcoI C'CATG_G
Cuts at: 513 1065 3111 513
Size: 552 2046 3619
Fragments arranged by size:
3619 2046 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6217
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6217
NotI GC'GGCC_GC
Cuts at: 3664 3664
Size: 6217
NspI r_CATG'y
Cuts at: 1132 1229 3575 5120 1132
Size: 97 2346 1545 2229
Fragments arranged by size:
2346 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6217
PciI A'CATG_T
Cuts at: 5116 5116
Size: 6217
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2417 2836 1108
Size: 1309 419 4489
Fragments arranged by size:
4489 1309 419
PmeI GTTT'AAAC
Cuts at: 3521 3521
Size: 6217
PmlI CAC'GTG
Cuts at: 2644 2644
Size: 6217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4286 4318 5799 5831 4286
Size: 32 1481 32 4672
Fragments arranged by size:
4672 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2833 2833
Size: 6217
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6217
PsiI TTA'TAA
Cuts at: 3787 3787
Size: 6217
PspOMI G'GGCC_C
Cuts at: 2913 3181 2913
Size: 268 5949
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2892 2924 2892
Size: 32 6185
PstI C_TGCA'G
Cuts at: 830 2295 3569 830
Size: 1465 1274 3478
Fragments arranged by size:
3478 1465 1274
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6217
PvuII CAG'CTG
Cuts at: 2290 3298 2290
Size: 1008 5209
SacI G_AGCT'C
Cuts at: 2616 2975 2616
Size: 359 5858
SalI G'TCGA_C
Cuts at: 1936 3559 1936
Size: 1623 4594
SapI GCTCTTCn'nnn_
Cuts at: 2604 2604
Size: 6217
SbfI CC_TGCA'GG
Cuts at: 3569 3569
Size: 6217
ScaI AGT'ACT
Cuts at: 2990 4465 6177 2990
Size: 1475 1712 3030
Fragments arranged by size:
3030 1712 1475
SexAI A'CCwGG_T
Cuts at: 2835 2835
Size: 6217
SfcI C'TryA_G
Cuts at: 826 1045 2291 3228 3508 3565 4700 5381 5572
Size: 219 1246 937 280 57 1135 681 191
Cuts at: 5572 826
Size: 1471
Fragments arranged by size:
1471 1246 1135 937 681 280 219 191
57
SfiI GGCCn_nnn'nGGCC
Cuts at: 3180 3180
Size: 6217
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6217
SmaI CCC'GGG
Cuts at: 1872 3544 1872
Size: 1672 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3275 3500 4321 5222 5484 820
Size: 197 926 1332 225 821 901 262 1553
Fragments arranged by size:
1553 1332 926 901 821 262 225 197
SnaBI TAC'GTA
Cuts at: 493 4008 493
Size: 3515 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6217
SphI G_CATG'C
Cuts at: 1132 3575 1132
Size: 2443 3774
SspI AAT'ATT
Cuts at: 5 52 4141 5
Size: 47 4089 2081
Fragments arranged by size:
4089 2081 47
StuI AGG'CCT
Cuts at: 1517 2937 1517
Size: 1420 4797
StyI C'CwwG_G
Cuts at: 513 1065 2367 3030 3111 3656 513
Size: 552 1302 663 81 545 3074
Fragments arranged by size:
3074 1302 663 552 545 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2061 2196 2456 2480 2869 4244 4261
Size: 1255 481 135 260 24 389 1375 17
Cuts at: 4261 4414 4599 5044 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1375 1255 481 445 389 260 185
153 135 24 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2100 2899 2988
Size: 274 80 33 51 1443 123 799 89
Cuts at: 2988 4463 6175 96
Size: 1475 1712 138
Fragments arranged by size:
1712 1475 1443 799 274 138 123 89
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6177 6209 6177
Size: 32 6185
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6217
XbaI T'CTAG_A
Cuts at: 3553 3553
Size: 6217
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6217
XmaI C'CCGG_G
Cuts at: 1870 3542 1870
Size: 1672 4545
XmnI GAAnn'nnTTC
Cuts at: 4346 4346
Size: 6217
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4025 276
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PspXI RsrII SacII SanDI SfoI SrfI SwaI XcmI