Restriction map for
pFN21AB5907
(Circular) (Six-base) MAPSORT of: pFN21AB5907.seq Check: 4593 from: 1 to: 6217
pFN21AB5907 6217 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4027 278
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2150 3560 1937
Size: 213 1410 4594
Fragments arranged by size:
4594 1410 213
Acc65I G'GTAC_C
Cuts at: 3538 3538
Size: 6217
AclI AA'CG_TT
Cuts at: 1563 4344 4717 1563
Size: 2781 373 3063
Fragments arranged by size:
3063 2781 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2140 2669 2685 4280 5664 5932 1632
Size: 508 529 16 1595 1384 268 1917
Fragments arranged by size:
1917 1595 1384 529 508 268 16
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6020
AflIII A'CryG_T
Cuts at: 1203 5116 6093 1203
Size: 3913 977 1327
Fragments arranged by size:
3913 1327 977
AgeI A'CCGG_T
Cuts at: 5048 5048
Size: 6217
AhdI GACnn_n'nnGTC
Cuts at: 4946 4946
Size: 6217
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2044 1184
Size: 860 5357
AlwNI CAG_nnn'CTG
Cuts at: 2065 2647 3960 5532 2065
Size: 582 1313 1572 2750
Fragments arranged by size:
2750 1572 1313 582
ApaLI G'TGCA_C
Cuts at: 2019 4274 5430 2019
Size: 2255 1156 2806
Fragments arranged by size:
2806 2255 1156
ApoI r'AATT_y
Cuts at: 1493 2620 2682 3526 3752 3904 6013 1493
Size: 1127 62 844 226 152 2109 1697
Fragments arranged by size:
2109 1697 1127 844 226 152 62
AseI AT'TA_AT
Cuts at: 160 1032 4771 160
Size: 872 3739 1606
Fragments arranged by size:
3739 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
AvaI C'yCGr_G
Cuts at: 1870 1943 2568 2580 2995 3542 1870
Size: 73 625 12 415 547 4545
Fragments arranged by size:
4545 625 547 415 73 12
AvrII C'CTAG_G
Cuts at: 2960 2960
Size: 6217
BamHI G'GATC_C
Cuts at: 3547 3914 3547
Size: 367 5850
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2369 3200 3305 3538 4993
Size: 325 217 627 582 831 105 233 1455
Cuts at: 4993 618
Size: 1842
Fragments arranged by size:
1842 1455 831 627 582 325 233 217
105
BanII G_rGCy'C
Cuts at: 1391 2699 3536 1391
Size: 1308 837 4072
Fragments arranged by size:
4072 1308 837
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5246
BbvCI CC'TCA_GC
Cuts at: 3334 3334
Size: 6217
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4408 4442 1903
Size: 34 2471 34 3678
Fragments arranged by size:
3678 2471 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4109 5319 4109
Size: 1210 5007
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6217
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2703 4828 136
Size: 107 122 71 631 605 1031 2125 1525
Fragments arranged by size:
2125 1525 1031 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6212 6212
Size: 6217
BlpI GC'TnA_GC
Cuts at: 2109 3634 2109
Size: 1525 4692
Bme1580I G_kGCm'C
Cuts at: 1790 2023 2372 4278 5434 1790
Size: 233 349 1906 1156 2573
Fragments arranged by size:
2573 1906 1156 349 233
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6217
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3948 4906 442
Size: 416 53 83 399 24 2531 958 1753
Fragments arranged by size:
2531 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6217
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2783 2783
Size: 6217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2279 2309 2480 2584 3108 3470 4877 1239
Size: 1040 30 171 104 524 362 1407 2579
Fragments arranged by size:
2579 1407 1040 524 362 171 104 30
Bpu10I CC'TnA_GC
Cuts at: 2114 3334 2114
Size: 1220 4997
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4342 5207 5505 4342
Size: 865 298 5054
Fragments arranged by size:
5054 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2333 2814 2846 4880 882
Size: 617 834 481 32 2034 2219
Fragments arranged by size:
2219 2034 834 617 481 32
BsaAI yAC'GTr
Cuts at: 493 1204 2616 2859 4008 493
Size: 711 1412 243 1149 2702
Fragments arranged by size:
2702 1412 1149 711 243
BsaBI GATnn'nnATC
Cuts at: 6211 6211
Size: 6217
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4024 4406 5041 275
Size: 53 83 186 936 2491 382 635 1451
Fragments arranged by size:
2491 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2732 4650 5048 5322 5469 5880 2732
Size: 1918 398 274 147 411 3069
Fragments arranged by size:
3069 1918 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2733 2763 2843 2873 1178
Size: 30 165 30 1330 30 80 30 4522
Fragments arranged by size:
4522 1330 165 80 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2288 2429 1186
Size: 1102 141 4974
Fragments arranged by size:
4974 1102 141
BseYI C'CCAG_C
Cuts at: 2365 5420 5916 2365
Size: 3055 496 2666
Fragments arranged by size:
3055 2666 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5644
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3667 4428 5456 1540
Size: 275 186 1666 761 1028 2301
Fragments arranged by size:
2301 1666 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2023 4278 4363 5434 2023
Size: 2255 85 1071 2806
Fragments arranged by size:
2806 2255 1071 85
BsmI GAATG_Cn'
Cuts at: 3728 3821 3728
Size: 93 6124
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2372 2699 3536 4278 4363 5434
Size: 399 233 349 327 837 742 85 1071
Cuts at: 5434 1391
Size: 2174
Fragments arranged by size:
2174 1071 837 742 399 349 327 233
85
BspEI T'CCGG_A
Cuts at: 5880 5880
Size: 6217
BspHI T'CATG_A
Cuts at: 4106 5836 4106
Size: 1730 4487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3572 844
Size: 2728 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2386 4104 1394
Size: 429 563 1718 3507
Fragments arranged by size:
3507 1718 563 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4712 4886 65
Size: 1183 3464 174 1396
Fragments arranged by size:
3464 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4861 5048 1700
Size: 110 3051 187 2869
Fragments arranged by size:
3051 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3312 96
Size: 3216 3001
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6217
BssSI C'ACGA_G
Cuts at: 4277 5289 4277
Size: 1012 5205
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6217
BstBI TT'CG_AA
Cuts at: 5021 5021
Size: 6217
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6217
BstXI CCAn_nnnn'nTGG
Cuts at: 2416 2512 2416
Size: 96 6121
BstYI r'GATC_y
Cuts at: 1972 3547 3914 4299 4316 5768 5854 5866 6212
Size: 1575 367 385 17 1452 86 12 346
Cuts at: 6212 1972
Size: 1977
Fragments arranged by size:
1977 1575 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2151 2151
Size: 6217
Bsu36I CC'TnA_GG
Cuts at: 2375 2375
Size: 6217
BtgI C'CryG_G
Cuts at: 513 1065 3100 513
Size: 552 2035 3630
Fragments arranged by size:
3630 2035 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5478
Fragments arranged by size:
5478 683 56
BtsI GCAGTG_nn'
Cuts at: 2574 3733 4527 4547 2574
Size: 1159 794 20 4244
Fragments arranged by size:
4244 1159 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6182
DraI TTT'AAA
Cuts at: 3521 3521
Size: 6217
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5224 809
Size: 507 3908 1802
Fragments arranged by size:
3908 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2137 2163 2502
Size: 54 1425 50 275 18 307 26 339
Cuts at: 2502 3664 4553 6182 8
Size: 1162 889 1629 43
Fragments arranged by size:
1629 1425 1162 889 339 307 275 54
50 43 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 3664 1537
Size: 275 1852 4090
Fragments arranged by size:
4090 1852 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2460 4147 1353
Size: 1107 1687 3423
Fragments arranged by size:
3423 1687 1107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2879 4804 5178 5324 387
Size: 1064 138 1290 1925 374 146 1280
Fragments arranged by size:
1925 1290 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2140 2279 2309 2480 2584 2669 2685
Size: 393 508 139 30 171 104 85 16
Cuts at: 2685 3108 3470 4280 4877 5664 5932 1239
Size: 423 362 810 597 787 268 1524
Fragments arranged by size:
1524 810 787 597 508 423 393 362
268 171 139 104 85 30 16
EcoO109I rG'GnC_Cy
Cuts at: 2331 2331
Size: 6217
EcoRI G'AATT_C
Cuts at: 3526 3904 3526
Size: 378 5839
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3478 3478
Size: 6217
FspI TGC'GCA
Cuts at: 4723 4723
Size: 6217
HaeII r_GCGC'y
Cuts at: 1678 3021 5364 1678
Size: 1343 2343 2531
Fragments arranged by size:
2531 2343 1343
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2684 2716 4858 4890 4932
Size: 32 135 32 1112 32 2142 32 42
Cuts at: 4932 4964 1373
Size: 32 2626
Fragments arranged by size:
2626 2142 1112 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3352 3561 3807 669
Size: 988 281 1414 209 246 3079
Fragments arranged by size:
3079 1414 988 281 246 209
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6217
HpaI GTT'AAC
Cuts at: 3807 3807
Size: 6217
KpnI G_GTAC'C
Cuts at: 3542 3542
Size: 6217
MfeI C'AATT_G
Cuts at: 2306 3816 2306
Size: 1510 4707
MluI A'CGCG_T
Cuts at: 6093 6093
Size: 6217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2397 2493 5331 5515 1305
Size: 1092 96 2838 184 2007
Fragments arranged by size:
2838 2007 1092 184 96
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2165 2504 10
Size: 54 1425 343 333 339 3723
Fragments arranged by size:
3723 1425 343 339 333 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4175 4534 4693 518
Size: 648 3009 359 159 2042
Fragments arranged by size:
3009 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2054 2062 2184 2395 3633 4310 5458 5703
Size: 448 8 122 211 1238 677 1148 245
Cuts at: 5703 1606
Size: 2120
Fragments arranged by size:
2120 1238 1148 677 448 245 211 122
8
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5665
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6217
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6217
NotI GC'GGCC_GC
Cuts at: 3664 3664
Size: 6217
NspI r_CATG'y
Cuts at: 1132 1229 3575 5120 1132
Size: 97 2346 1545 2229
Fragments arranged by size:
2346 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6217
PciI A'CATG_T
Cuts at: 5116 5116
Size: 6217
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6217
PfoI T'CCnGG_A
Cuts at: 2640 2640
Size: 6217
PmeI GTTT'AAAC
Cuts at: 3521 3521
Size: 6217
PmlI CAC'GTG
Cuts at: 2859 2859
Size: 6217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4286 4318 5799 5831 4286
Size: 32 1481 32 4672
Fragments arranged by size:
4672 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6217
PsiI TTA'TAA
Cuts at: 3787 3787
Size: 6217
PstI C_TGCA'G
Cuts at: 830 2033 2358 3569 830
Size: 1203 325 1211 3478
Fragments arranged by size:
3478 1211 1203 325
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6217
PvuII CAG'CTG
Cuts at: 2054 2062 2184 2395 2054
Size: 8 122 211 5876
Fragments arranged by size:
5876 211 122 8
SalI G'TCGA_C
Cuts at: 1936 3559 1936
Size: 1623 4594
SbfI CC_TGCA'GG
Cuts at: 3569 3569
Size: 6217
ScaI AGT'ACT
Cuts at: 4465 6177 4465
Size: 1712 4505
SfcI C'TryA_G
Cuts at: 826 1045 2029 2354 2428 3565 4700 5381 5572
Size: 219 984 325 74 1137 1135 681 191
Cuts at: 5572 826
Size: 1471
Fragments arranged by size:
1471 1137 1135 984 681 325 219 191
74
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6217
SmaI CCC'GGG
Cuts at: 1872 3544 1872
Size: 1672 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4321 5222 5484 820
Size: 197 926 2378 901 262 1553
Fragments arranged by size:
2378 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4008 493
Size: 3515 2702
SpeI A'CTAG_T
Cuts at: 152 2746 152
Size: 2594 3623
SphI G_CATG'C
Cuts at: 1132 3575 1132
Size: 2443 3774
SspI AAT'ATT
Cuts at: 5 52 4141 5
Size: 47 4089 2081
Fragments arranged by size:
4089 2081 47
StuI AGG'CCT
Cuts at: 1517 3165 1517
Size: 1648 4569
StyI C'CwwG_G
Cuts at: 513 1065 2313 2960 3656 513
Size: 552 1248 647 696 3074
Fragments arranged by size:
3074 1248 696 647 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2258 2861 4244 4261 4414 4599 5044
Size: 1255 678 603 1383 17 153 185 445
Cuts at: 5044 325
Size: 1498
Fragments arranged by size:
1498 1383 1255 678 603 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2991 3312 3420
Size: 274 80 33 51 1443 1014 321 108
Cuts at: 3420 4463 6175 96
Size: 1043 1712 138
Fragments arranged by size:
1712 1443 1043 1014 321 274 138 108
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2843 2875 6177 6209 2843
Size: 32 3302 32 2851
Fragments arranged by size:
3302 2851 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6217
XbaI T'CTAG_A
Cuts at: 3553 3553
Size: 6217
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6217
XmaI C'CCGG_G
Cuts at: 1870 3542 1870
Size: 1672 4545
XmnI GAAnn'nnTTC
Cuts at: 4346 4346
Size: 6217
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4025 276
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI
BsiWI BsmBI DraIII EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI