Restriction map for
pFN21AB5903
(Circular) (Six-base) MAPSORT of: pFN21AB5903.seq Check: 8522 from: 1 to: 6208
pFN21AB5903 6208 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4018 278
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3551 1937
Size: 1614 4594
Acc65I G'GTAC_C
Cuts at: 3529 3529
Size: 6208
AclI AA'CG_TT
Cuts at: 1563 4335 4708 1563
Size: 2772 373 3063
Fragments arranged by size:
3063 2772 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2976 3258 4271 5655 5923 1632
Size: 1344 282 1013 1384 268 1917
Fragments arranged by size:
1917 1384 1344 1013 282 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6011
AflIII A'CryG_T
Cuts at: 1203 5107 6084 1203
Size: 3904 977 1327
Fragments arranged by size:
3904 1327 977
AgeI A'CCGG_T
Cuts at: 5039 5039
Size: 6208
AhdI GACnn_n'nnGTC
Cuts at: 4937 4937
Size: 6208
AleI CACnn'nnGTG
Cuts at: 1166 2228 1166
Size: 1062 5146
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6208
AlwNI CAG_nnn'CTG
Cuts at: 3951 5523 3951
Size: 1572 4636
ApaLI G'TGCA_C
Cuts at: 4265 5421 4265
Size: 1156 5052
ApoI r'AATT_y
Cuts at: 1493 2024 3517 3743 3895 6004 1493
Size: 531 1493 226 152 2109 1697
Fragments arranged by size:
2109 1697 1493 531 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4762 160
Size: 872 3730 1606
Fragments arranged by size:
3730 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
AvaI C'yCGr_G
Cuts at: 1870 1943 2068 2328 2471 3533 1870
Size: 73 125 260 143 1062 4545
Fragments arranged by size:
4545 1062 260 143 125 73
BamHI G'GATC_C
Cuts at: 3538 3905 3538
Size: 367 5841
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3529 4984 618
Size: 325 217 627 1742 1455 1842
Fragments arranged by size:
1842 1742 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2513 3527 1391
Size: 1122 1014 4072
Fragments arranged by size:
4072 1122 1014
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3240 928
Size: 971 1341 3896
Fragments arranged by size:
3896 1341 971
BbvCI CC'TCA_GC
Cuts at: 2103 2103
Size: 6208
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4399 4433 1903
Size: 34 2462 34 3678
Fragments arranged by size:
3678 2462 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4100 5310 4100
Size: 1210 4998
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6208
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2086 2475 4819
Size: 107 122 71 631 605 414 389 2344
Cuts at: 4819 136
Size: 1525
Fragments arranged by size:
2344 1525 631 605 414 389 122 107
71
BglII A'GATC_T
Cuts at: 6203 6203
Size: 6208
BlpI GC'TnA_GC
Cuts at: 3625 3625
Size: 6208
Bme1580I G_kGCm'C
Cuts at: 1790 2121 2471 4269 5425 1790
Size: 331 350 1798 1156 2573
Fragments arranged by size:
2573 1798 1156 350 331
BmgBI CAC'GTC
Cuts at: 1318 2353 1318
Size: 1035 5173
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2931 3028 3939
Size: 416 53 83 399 24 1514 97 911
Cuts at: 3939 4897 442
Size: 958 1753
Fragments arranged by size:
1753 1514 958 911 416 399 97 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3459 4868 1239
Size: 2220 1409 2579
Fragments arranged by size:
2579 2220 1409
Bpu10I CC'TnA_GC
Cuts at: 2103 2103
Size: 6208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2222 3274 4333 5198 5496 2222
Size: 1052 1059 865 298 2934
Fragments arranged by size:
2934 1059 1052 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2573 2680 4871 882
Size: 617 1074 107 2191 2219
Fragments arranged by size:
2219 2191 1074 617 107
BsaAI yAC'GTr
Cuts at: 493 1204 2821 3999 493
Size: 711 1617 1178 2702
Fragments arranged by size:
2702 1617 1178 711
BsaBI GATnn'nnATC
Cuts at: 6202 6202
Size: 6208
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2300 2523 4015 4397
Size: 53 83 186 936 767 223 1492 382
Cuts at: 4397 5032 275
Size: 635 1451
Fragments arranged by size:
1492 1451 936 767 635 382 223 186
83 53
BsaWI w'CCGG_w
Cuts at: 4641 5039 5313 5460 5871 4641
Size: 398 274 147 411 4978
Fragments arranged by size:
4978 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2660 2690 1178
Size: 30 165 30 1257 30 4696
Fragments arranged by size:
4696 1257 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3456 1186
Size: 2270 3938
BseYI C'CCAG_C
Cuts at: 2559 2856 5411 5907 2559
Size: 297 2555 496 2860
Fragments arranged by size:
2860 2555 496 297
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1995 1163
Size: 573 259 5376
Fragments arranged by size:
5376 573 259
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2158 3658 4419 5447 1540
Size: 275 186 157 1500 761 1028 2301
Fragments arranged by size:
2301 1500 1028 761 275 186 157
BsiHKAI G_wGCw'C
Cuts at: 2601 4269 4354 5425 2601
Size: 1668 85 1071 3384
Fragments arranged by size:
3384 1668 1071 85
BsmI GAATG_Cn'
Cuts at: 3170 3719 3812 3170
Size: 549 93 5566
Fragments arranged by size:
5566 549 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2414 2414
Size: 6208
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2471 2513 2601 3527 4269 4354
Size: 399 331 350 42 88 926 742 85
Cuts at: 4354 5425 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 926 742 399 350 331 88
85 42
BspEI T'CCGG_A
Cuts at: 5871 5871
Size: 6208
BspHI T'CATG_A
Cuts at: 4097 5827 4097
Size: 1730 4478
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2664 3563 844
Size: 1820 899 3489
Fragments arranged by size:
3489 1820 899
BsrBI CCG'CTC
Cuts at: 1394 1823 4095 1394
Size: 429 2272 3507
Fragments arranged by size:
3507 2272 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2170 4703 4877 65
Size: 1183 922 2533 174 1396
Fragments arranged by size:
2533 1396 1183 922 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2157 4852 5039 1700
Size: 110 347 2695 187 2869
Fragments arranged by size:
2869 2695 347 187 110
BsrGI T'GTAC_A
Cuts at: 96 2167 96
Size: 2071 4137
BssHII G'CGCG_C
Cuts at: 1925 2572 1925
Size: 647 5561
BssSI C'ACGA_G
Cuts at: 4268 5280 4268
Size: 1012 5196
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2116 794
Size: 1322 4886
BstBI TT'CG_AA
Cuts at: 5012 5012
Size: 6208
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6208
BstXI CCAn_nnnn'nTGG
Cuts at: 2230 2230
Size: 6208
BstYI r'GATC_y
Cuts at: 1972 3538 3905 4290 4307 5759 5845 5857 6203
Size: 1566 367 385 17 1452 86 12 346
Cuts at: 6203 1972
Size: 1977
Fragments arranged by size:
1977 1566 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2721 2721
Size: 6208
BtgI C'CryG_G
Cuts at: 513 1065 2280 513
Size: 552 1215 4441
Fragments arranged by size:
4441 1215 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5469
Fragments arranged by size:
5469 683 56
BtsI GCAGTG_nn'
Cuts at: 3724 4518 4538 3724
Size: 794 20 5394
Fragments arranged by size:
5394 794 20
ClaI AT'CG_AT
Cuts at: 1554 2826 1554
Size: 1272 4936
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6173
DraI TTT'AAA
Cuts at: 3019 3512 3019
Size: 493 5715
DraIII CAC_nnn'GTG
Cuts at: 2605 2605
Size: 6208
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2056 5215 809
Size: 507 740 3159 1802
Fragments arranged by size:
3159 1802 740 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2155 2376 3655
Size: 54 1425 50 275 18 325 221 1279
Cuts at: 3655 4544 6173 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1279 889 325 275 221 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2155 3655 1537
Size: 275 343 1500 4090
Fragments arranged by size:
4090 1500 343 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2222 2261 2414 2492 2498 3000 3223 4138
Size: 869 39 153 78 6 502 223 915
Cuts at: 4138 1353
Size: 3423
Fragments arranged by size:
3423 915 869 502 223 153 78 39
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4795 5169 5315 387
Size: 1064 138 3206 374 146 1280
Fragments arranged by size:
3206 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2976 3258 3459 4271 4868 5655 5923
Size: 393 1344 282 201 812 597 787 268
Cuts at: 5923 1239
Size: 1524
Fragments arranged by size:
1524 1344 812 787 597 393 282 268
201
EcoO109I rG'GnC_Cy
Cuts at: 2098 2098
Size: 6208
EcoRI G'AATT_C
Cuts at: 3517 3895 3517
Size: 378 5830
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3482 3482
Size: 6208
FspI TGC'GCA
Cuts at: 2660 4714 2660
Size: 2054 4154
HaeII r_GCGC'y
Cuts at: 1678 2248 5355 1678
Size: 570 3107 2531
Fragments arranged by size:
3107 2531 570
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2917 2949 4849 4881 4923
Size: 32 135 32 1345 32 1900 32 42
Cuts at: 4923 4955 1373
Size: 32 2626
Fragments arranged by size:
2626 1900 1345 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3552 3798 669
Size: 988 281 1614 246 3079
Fragments arranged by size:
3079 1614 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3422 748
Size: 2674 3534
HpaI GTT'AAC
Cuts at: 3798 3798
Size: 6208
KpnI G_GTAC'C
Cuts at: 3533 3533
Size: 6208
MfeI C'AATT_G
Cuts at: 3807 3807
Size: 6208
MluI A'CGCG_T
Cuts at: 6084 6084
Size: 6208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5322 5506 1305
Size: 4017 184 2007
Fragments arranged by size:
4017 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 10
Size: 54 1425 343 546 3840
Fragments arranged by size:
3840 1425 546 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2228 2552 2636 3374 4166 4525 4684
Size: 648 1062 324 84 738 792 359 159
Cuts at: 4684 518
Size: 2042
Fragments arranged by size:
2042 1062 792 738 648 359 324 159
84
MspA1I CmG'CkG
Cuts at: 1606 2064 2137 3624 4301 5449 5694 1606
Size: 458 73 1487 677 1148 245 2120
Fragments arranged by size:
2120 1487 1148 677 458 245 73
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5656
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6208
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6208
NotI GC'GGCC_GC
Cuts at: 3655 3655
Size: 6208
NspI r_CATG'y
Cuts at: 1132 1229 2545 2869 3566 5111 1132
Size: 97 1316 324 697 1545 2229
Fragments arranged by size:
2229 1545 1316 697 324 97
PasI CC'CwG_GG
Cuts at: 1348 2083 1348
Size: 735 5473
PciI A'CATG_T
Cuts at: 5107 5107
Size: 6208
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2310 3403 1108
Size: 1202 1093 3913
Fragments arranged by size:
3913 1202 1093
PmeI GTTT'AAAC
Cuts at: 3512 3512
Size: 6208
PmlI CAC'GTG
Cuts at: 2821 2821
Size: 6208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2714 2746 3247 3279 4277 4309 5790 5822 2714
Size: 32 501 32 998 32 1481 32 3100
Fragments arranged by size:
3100 1481 998 501 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2098 2098
Size: 6208
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6208
PsiI TTA'TAA
Cuts at: 3778 3778
Size: 6208
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2935 2967 2935
Size: 32 6176
PstI C_TGCA'G
Cuts at: 830 3216 3560 830
Size: 2386 344 3478
Fragments arranged by size:
3478 2386 344
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6208
SalI G'TCGA_C
Cuts at: 1936 3550 1936
Size: 1614 4594
SbfI CC_TGCA'GG
Cuts at: 3560 3560
Size: 6208
ScaI AGT'ACT
Cuts at: 4456 6168 4456
Size: 1712 4496
SfcI C'TryA_G
Cuts at: 826 1045 3212 3286 3556 4691 5372 5563 826
Size: 219 2167 74 270 1135 681 191 1471
Fragments arranged by size:
2167 1471 1135 681 270 219 191 74
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6208
SmaI CCC'GGG
Cuts at: 1872 3535 1872
Size: 1663 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2237 3253 4312 5213 5475 820
Size: 197 926 294 1016 1059 901 262 1553
Fragments arranged by size:
1553 1059 1016 926 901 294 262 197
SnaBI TAC'GTA
Cuts at: 493 3999 493
Size: 3506 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6208
SphI G_CATG'C
Cuts at: 1132 2869 3566 1132
Size: 1737 697 3774
Fragments arranged by size:
3774 1737 697
SspI AAT'ATT
Cuts at: 5 52 4132 5
Size: 47 4080 2081
Fragments arranged by size:
4080 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6208
StyI C'CwwG_G
Cuts at: 513 1065 3647 513
Size: 552 2582 3074
Fragments arranged by size:
3074 2582 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2302 4235 4252 4405 4590 5035 325
Size: 1255 722 1933 17 153 185 445 1498
Fragments arranged by size:
1933 1498 1255 722 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2167 2771 4454
Size: 274 80 33 51 1443 190 604 1683
Cuts at: 4454 6166 96
Size: 1712 138
Fragments arranged by size:
1712 1683 1443 604 274 190 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2225 2257 2660 2692 6168 6200 2225
Size: 32 403 32 3476 32 2233
Fragments arranged by size:
3476 2233 403 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6208
XbaI T'CTAG_A
Cuts at: 3544 3544
Size: 6208
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6208
XmaI C'CCGG_G
Cuts at: 1870 3533 1870
Size: 1663 4545
XmnI GAAnn'nnTTC
Cuts at: 2255 3096 3237 3325 4337 2255
Size: 841 141 88 1012 4126
Fragments arranged by size:
4126 1012 841 141 88
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4016 276
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstZ17I EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PspOMI PspXI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI