Restriction map for
pFN21AB5902
(Circular) (Six-base) MAPSORT of: pFN21AB5902.seq Check: 1315 from: 1 to: 6280
pFN21AB5902 6280 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3008 3008
Size: 6280
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4090 278
Size: 53 83 186 936 2554 2468
Fragments arranged by size:
2554 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3623 1937
Size: 1686 4594
Acc65I G'GTAC_C
Cuts at: 3601 3601
Size: 6280
AclI AA'CG_TT
Cuts at: 1563 4407 4780 1563
Size: 2844 373 3063
Fragments arranged by size:
3063 2844 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2560 4343 5727 5995 1632
Size: 928 1783 1384 268 1917
Fragments arranged by size:
1917 1783 1384 928 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6083
AflIII A'CryG_T
Cuts at: 1203 5179 6156 1203
Size: 3976 977 1327
Fragments arranged by size:
3976 1327 977
AgeI A'CCGG_T
Cuts at: 5111 5111
Size: 6280
AhdI GACnn_n'nnGTC
Cuts at: 5009 5009
Size: 6280
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6280
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3048 1184
Size: 1864 4416
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2003 2035 2003
Size: 32 6248
AlwNI CAG_nnn'CTG
Cuts at: 2023 2084 2600 3128 4023 5595 2023
Size: 61 516 528 895 1572 2708
Fragments arranged by size:
2708 1572 895 528 516 61
ApaI G_GGCC'C
Cuts at: 2107 2286 2718 2107
Size: 179 432 5669
Fragments arranged by size:
5669 432 179
ApaLI G'TGCA_C
Cuts at: 2456 4337 5493 2456
Size: 1881 1156 3243
Fragments arranged by size:
3243 1881 1156
ApoI r'AATT_y
Cuts at: 1493 3589 3815 3967 6076 1493
Size: 2096 226 152 2109 1697
Fragments arranged by size:
2109 2096 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4834 160
Size: 872 3802 1606
Fragments arranged by size:
3802 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6280
AvaI C'yCGr_G
Cuts at: 1870 1943 3551 3557 3563 3605 1870
Size: 73 1608 6 6 42 4545
Fragments arranged by size:
4545 1608 73 42 6 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3108 3141 3108
Size: 33 6247
BamHI G'GATC_C
Cuts at: 3089 3610 3977 3089
Size: 521 367 5392
Fragments arranged by size:
5392 521 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2266 3305 3332 3601 5056
Size: 325 217 627 479 1039 27 269 1455
Cuts at: 5056 618
Size: 1842
Fragments arranged by size:
1842 1455 1039 627 479 325 269 217
27
BanII G_rGCy'C
Cuts at: 1391 2107 2119 2286 2718 2796 3155 3328 3552
Size: 716 12 167 432 78 359 173 224
Cuts at: 3552 3558 3564 3599 1391
Size: 6 6 35 4072
Fragments arranged by size:
4072 716 432 359 224 173 167 78
35 12 6 6
BbeI G_GCGC'C
Cuts at: 2270 2270
Size: 6280
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3436 928
Size: 971 1537 3772
Fragments arranged by size:
3772 1537 971
BbvCI CC'TCA_GC
Cuts at: 2085 2085
Size: 6280
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4471 4505 1903
Size: 34 2534 34 3678
Fragments arranged by size:
3678 2534 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4172 5382 4172
Size: 1210 5070
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6280
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4891 136
Size: 107 122 71 631 605 3219 1525
Fragments arranged by size:
3219 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6275 6275
Size: 6280
BlpI GC'TnA_GC
Cuts at: 2309 3697 2309
Size: 1388 4892
Bme1580I G_kGCm'C
Cuts at: 1790 2107 2286 2460 2718 3310 3337 4341 5497
Size: 317 179 174 258 592 27 1004 1156
Cuts at: 5497 1790
Size: 2573
Fragments arranged by size:
2573 1156 1004 592 317 258 179 174
27
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6280
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2563 4011 4969
Size: 416 53 83 399 24 1146 1448 958
Cuts at: 4969 442
Size: 1753
Fragments arranged by size:
1753 1448 1146 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6280
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2043 2057 2138 2810 2841 2955 3105 3218
Size: 804 14 81 672 31 114 150 113
Cuts at: 3218 3423 3530 4940 1239
Size: 205 107 1410 2579
Fragments arranged by size:
2579 1410 804 672 205 150 114 113
107 81 31 14
Bpu10I CC'TnA_GC
Cuts at: 2085 2085
Size: 6280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4405 5270 5568 4405
Size: 865 298 5117
Fragments arranged by size:
5117 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4943 882
Size: 617 3444 2219
Fragments arranged by size:
3444 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4071 493
Size: 711 2867 2702
Fragments arranged by size:
2867 2702 711
BsaBI GATnn'nnATC
Cuts at: 6274 6274
Size: 6280
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2267 2433 4087 4469
Size: 53 83 186 936 734 166 1654 382
Cuts at: 4469 5104 275
Size: 635 1451
Fragments arranged by size:
1654 1451 936 734 635 382 186 166
83 53
BsaWI w'CCGG_w
Cuts at: 2613 3520 3544 4713 5111 5385 5532 5943 2613
Size: 907 24 1169 398 274 147 411 2950
Fragments arranged by size:
2950 1169 907 411 398 274 147 24
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2003 2033 3049 3079 3261
Size: 30 165 30 600 30 1016 30 182
Cuts at: 3261 3291 1178
Size: 30 4167
Fragments arranged by size:
4167 1016 600 182 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2263 2892 3190 3518 1186
Size: 1077 629 298 328 3948
Fragments arranged by size:
3948 1077 629 328 298
BseYI C'CCAG_C
Cuts at: 2124 2416 2593 2987 3213 3225 3340 5483 5979
Size: 292 177 394 226 12 115 2143 496
Cuts at: 5979 2124
Size: 2425
Fragments arranged by size:
2425 2143 496 394 292 226 177 115
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5707
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2383 3730 4491 5519 1540
Size: 275 186 382 1347 761 1028 2301
Fragments arranged by size:
2301 1347 1028 761 382 275 186
BsiHKAI G_wGCw'C
Cuts at: 2460 3155 4341 4426 5497 2460
Size: 695 1186 85 1071 3243
Fragments arranged by size:
3243 1186 1071 695 85
BsmI GAATG_Cn'
Cuts at: 3791 3884 3791
Size: 93 6187
BsmBI CGTCTCn'nnnn_
Cuts at: 2761 2761
Size: 6280
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2107 2119 2286 2460 2718 2796 3155
Size: 399 317 12 167 174 258 78 359
Cuts at: 3155 3310 3328 3337 3552 3558 3564 3599 4341
Size: 155 18 9 215 6 6 35 742
Cuts at: 4341 4426 5497 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 359 317 258 215
174 167 155 85 78 35 18 12
9 6 6
BspEI T'CCGG_A
Cuts at: 2613 5943 2613
Size: 3330 2950
BspHI T'CATG_A
Cuts at: 4169 5899 4169
Size: 1730 4550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2816 3008 3635 844
Size: 1972 192 627 3489
Fragments arranged by size:
3489 1972 627 192
BsrBI CCG'CTC
Cuts at: 1394 1823 4167 1394
Size: 429 2344 3507
Fragments arranged by size:
3507 2344 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3242 4775 4949 65
Size: 1183 1994 1533 174 1396
Fragments arranged by size:
1994 1533 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2382 2435 4924 5111 1700
Size: 110 572 53 2489 187 2869
Fragments arranged by size:
2869 2489 572 187 110 53
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6280
BssHII G'CGCG_C
Cuts at: 1925 2425 1925
Size: 500 5780
BssSI C'ACGA_G
Cuts at: 4340 5352 4340
Size: 1012 5268
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6280
BstBI TT'CG_AA
Cuts at: 5084 5084
Size: 6280
BstEII G'GTnAC_C
Cuts at: 1184 3081 1184
Size: 1897 4383
BstXI CCAn_nnnn'nTGG
Cuts at: 3317 3317
Size: 6280
BstYI r'GATC_y
Cuts at: 1972 2158 2534 3089 3610 3977 4362 4379 5831
Size: 186 376 555 521 367 385 17 1452
Cuts at: 5831 5917 5929 6275 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 555 521 385 376 367 346
186 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2462 2462
Size: 6280
BtgI C'CryG_G
Cuts at: 513 1065 2451 2703 513
Size: 552 1386 252 4090
Fragments arranged by size:
4090 1386 552 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5541
Fragments arranged by size:
5541 683 56
BtsI GCAGTG_nn'
Cuts at: 2886 3282 3796 4590 4610 2886
Size: 396 514 794 20 4556
Fragments arranged by size:
4556 794 514 396 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6280
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6245
DraI TTT'AAA
Cuts at: 3584 3584
Size: 6280
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5287 809
Size: 507 3971 1802
Fragments arranged by size:
3971 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2233 2380 3727
Size: 54 1425 50 275 18 403 147 1347
Cuts at: 3727 4616 6245 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1347 889 403 275 147 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2380 3727 1537
Size: 275 568 1347 4090
Fragments arranged by size:
4090 1347 568 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2170 2687 4210 1353
Size: 817 517 1523 3423
Fragments arranged by size:
3423 1523 817 517
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4867 5241 5387 387
Size: 1064 138 3278 374 146 1280
Fragments arranged by size:
3278 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3153 3153
Size: 6280
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2043 2057 2138 2560 2810 2841 2955
Size: 393 411 14 81 422 250 31 114
Cuts at: 2955 3105 3218 3423 3530 4343 4940 5727 5995
Size: 150 113 205 107 813 597 787 268
Cuts at: 5995 1239
Size: 1524
Fragments arranged by size:
1524 813 787 597 422 411 393 268
250 205 150 114 113 107 81 31
14
EcoNI CCTnn'n_nnAGG
Cuts at: 2504 2910 2504
Size: 406 5874
EcoO109I rG'GnC_Cy
Cuts at: 2089 2282 2289 2318 2715 2871 2915 2991 3188
Size: 193 7 29 397 156 44 76 197
Cuts at: 3188 2089
Size: 5181
Fragments arranged by size:
5181 397 197 193 156 76 44 29
7
EcoRI G'AATT_C
Cuts at: 3589 3967 3589
Size: 378 5902
FspI TGC'GCA
Cuts at: 2333 4786 2333
Size: 2453 3827
HaeII r_GCGC'y
Cuts at: 1678 2270 5427 1678
Size: 592 3157 2531
Fragments arranged by size:
3157 2531 592
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2169 2201 2302 2334 2625
Size: 32 135 32 597 32 101 32 291
Cuts at: 2625 2657 3152 3184 3463 3495 4921 4953 4995
Size: 32 495 32 279 32 1426 32 42
Cuts at: 4995 5027 1373
Size: 32 2626
Fragments arranged by size:
2626 1426 597 495 291 279 135 101
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3624 3870 669
Size: 988 281 1686 246 3079
Fragments arranged by size:
3079 1686 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2674 748
Size: 1926 4354
HpaI GTT'AAC
Cuts at: 3870 3870
Size: 6280
KasI G'GCGC_C
Cuts at: 2266 2266
Size: 6280
KpnI G_GTAC'C
Cuts at: 3605 3605
Size: 6280
MfeI C'AATT_G
Cuts at: 3879 3879
Size: 6280
MluI A'CGCG_T
Cuts at: 6156 6156
Size: 6280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2574 5394 5578 1305
Size: 1269 2820 184 2007
Fragments arranged by size:
2820 2007 1269 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4458
Fragments arranged by size:
4458 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3059 4238 4597 4756 518
Size: 648 1893 1179 359 159 2042
Fragments arranged by size:
2042 1893 1179 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2222 2337 2368 2453 3244 3696 4373 5521
Size: 616 115 31 85 791 452 677 1148
Cuts at: 5521 5766 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 791 677 616 452 245 115
85 31
NaeI GCC'GGC
Cuts at: 2384 2437 2384
Size: 53 6227
NarI GG'CG_CC
Cuts at: 2267 2267
Size: 6280
NcoI C'CATG_G
Cuts at: 513 1065 2703 513
Size: 552 1638 4090
Fragments arranged by size:
4090 1638 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6280
NgoMIV G'CCGG_C
Cuts at: 2382 2435 2382
Size: 53 6227
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6280
NotI GC'GGCC_GC
Cuts at: 3727 3727
Size: 6280
NspI r_CATG'y
Cuts at: 1132 1229 3638 5183 1132
Size: 97 2409 1545 2229
Fragments arranged by size:
2409 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2718 3094 1348
Size: 1370 376 4534
Fragments arranged by size:
4534 1370 376
PciI A'CATG_T
Cuts at: 5179 5179
Size: 6280
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2210 2242 3128 1108
Size: 1102 32 886 4260
Fragments arranged by size:
4260 1102 886 32
PfoI T'CCnGG_A
Cuts at: 2154 2320 2643 2154
Size: 166 323 5791
Fragments arranged by size:
5791 323 166
PmeI GTTT'AAAC
Cuts at: 3584 3584
Size: 6280
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2003 2035 3012 3044 4349 4381 5862 5894 2003
Size: 32 977 32 1305 32 1481 32 2389
Fragments arranged by size:
2389 1481 1305 977 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2089 2318 2871 2991 3188 2089
Size: 229 553 120 197 5181
Fragments arranged by size:
5181 553 229 197 120
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6280
PsiI TTA'TAA
Cuts at: 3850 3850
Size: 6280
PspOMI G'GGCC_C
Cuts at: 2103 2282 2714 2103
Size: 179 432 5669
Fragments arranged by size:
5669 432 179
PstI C_TGCA'G
Cuts at: 830 2043 2138 2355 3018 3632 830
Size: 1213 95 217 663 614 3478
Fragments arranged by size:
3478 1213 663 614 217 95
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6280
PvuII CAG'CTG
Cuts at: 2368 3244 2368
Size: 876 5404
SacI G_AGCT'C
Cuts at: 3155 3155
Size: 6280
SacII CC_GC'GG
Cuts at: 2454 2454
Size: 6280
SalI G'TCGA_C
Cuts at: 1936 3622 1936
Size: 1686 4594
SanDI GG'GwC_CC
Cuts at: 2318 2871 2318
Size: 553 5727
SapI GCTCTTCn'nnn_
Cuts at: 2170 2170
Size: 6280
SbfI CC_TGCA'GG
Cuts at: 3632 3632
Size: 6280
ScaI AGT'ACT
Cuts at: 4528 6240 4528
Size: 1712 4568
SfcI C'TryA_G
Cuts at: 826 1045 2039 2134 2351 2737 3014 3628 4763
Size: 219 994 95 217 386 277 614 1135
Cuts at: 4763 5444 5635 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 994 681 614 386 277 219
217 191 95
SfoI GGC'GCC
Cuts at: 2268 2268
Size: 6280
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6280
SgrAI Cr'CCGG_yG
Cuts at: 1700 2435 1700
Size: 735 5545
SmaI CCC'GGG
Cuts at: 1872 3607 1872
Size: 1735 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4384 5285 5547 820
Size: 197 926 2441 901 262 1553
Fragments arranged by size:
2441 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4071 493
Size: 3578 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6280
SphI G_CATG'C
Cuts at: 1132 3638 1132
Size: 2506 3774
SspI AAT'ATT
Cuts at: 5 52 4204 5
Size: 47 4152 2081
Fragments arranged by size:
4152 2081 47
StuI AGG'CCT
Cuts at: 1517 2328 2510 1517
Size: 811 182 5287
Fragments arranged by size:
5287 811 182
StyI C'CwwG_G
Cuts at: 513 1065 2703 3191 3230 3370 3719 513
Size: 552 1638 488 39 140 349 3074
Fragments arranged by size:
3074 1638 552 488 349 140 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2041 4307 4324 4477 4662 5107 325
Size: 1255 461 2266 17 153 185 445 1498
Fragments arranged by size:
2266 1498 1255 461 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3279 4526 6238
Size: 274 80 33 51 1443 1302 1247 1712
Cuts at: 6238 96
Size: 138
Fragments arranged by size:
1712 1443 1302 1247 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3049 3081 3261 3293 6240 6272 3049
Size: 32 180 32 2947 32 3057
Fragments arranged by size:
3057 2947 180 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2555 2875 1316
Size: 1239 320 4721
Fragments arranged by size:
4721 1239 320
XbaI T'CTAG_A
Cuts at: 3616 3616
Size: 6280
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2984 3043 2984
Size: 59 6221
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6280
XmaI C'CCGG_G
Cuts at: 1870 3605 1870
Size: 1735 4545
XmnI GAAnn'nnTTC
Cuts at: 4409 4409
Size: 6280
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4088 276
Size: 53 83 186 936 2554 2468
Fragments arranged by size:
2554 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BfrBI BplI BsiWI BstZ17I DraIII
EcoRV FalI FseI FspAI NruI NsiI PacI PmlI
PspXI PsrI RsrII SexAI SfiI SrfI SwaI