Restriction map for
pFN21AB5896
(Circular) (Six-base) MAPSORT of: pFN21AB5896.seq Check: 8207 from: 1 to: 6277
pFN21AB5896 6277 bp
With 182 enzymes: SgfI *
December 3, 2014 10:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4087 278
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3276 3620 1937
Size: 1339 344 4594
Fragments arranged by size:
4594 1339 344
Acc65I G'GTAC_C
Cuts at: 3598 3598
Size: 6277
AclI AA'CG_TT
Cuts at: 1563 4404 4777 1563
Size: 2841 373 3063
Fragments arranged by size:
3063 2841 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3377 4340 5724 5992 1632
Size: 1745 963 1384 268 1917
Fragments arranged by size:
1917 1745 1384 963 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6080
AflIII A'CryG_T
Cuts at: 1203 2632 5176 6153 1203
Size: 1429 2544 977 1327
Fragments arranged by size:
2544 1429 1327 977
AgeI A'CCGG_T
Cuts at: 5108 5108
Size: 6277
AhdI GACnn_n'nnGTC
Cuts at: 2095 3283 5006 2095
Size: 1188 1723 3366
Fragments arranged by size:
3366 1723 1188
AleI CACnn'nnGTG
Cuts at: 1166 3002 1166
Size: 1836 4441
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6277
AlwNI CAG_nnn'CTG
Cuts at: 4020 5592 4020
Size: 1572 4705
ApaLI G'TGCA_C
Cuts at: 4334 5490 4334
Size: 1156 5121
ApoI r'AATT_y
Cuts at: 1493 2223 2285 3586 3812 3964 6073 1493
Size: 730 62 1301 226 152 2109 1697
Fragments arranged by size:
2109 1697 1301 730 226 152 62
AseI AT'TA_AT
Cuts at: 160 1032 2844 4831 160
Size: 872 1812 1987 1606
Fragments arranged by size:
1987 1812 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
AvaI C'yCGr_G
Cuts at: 1870 1943 3602 1870
Size: 73 1659 4545
Fragments arranged by size:
4545 1659 73
BamHI G'GATC_C
Cuts at: 3607 3974 3607
Size: 367 5910
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3235 3598 5053 618
Size: 325 217 627 1448 363 1455 1842
Fragments arranged by size:
1842 1455 1448 627 363 325 217
BanII G_rGCy'C
Cuts at: 1391 2314 3596 1391
Size: 923 1282 4072
Fragments arranged by size:
4072 1282 923
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2681 928
Size: 971 782 4524
Fragments arranged by size:
4524 971 782
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4468 4502 1903
Size: 34 2531 34 3678
Fragments arranged by size:
3678 2531 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4169 5379 4169
Size: 1210 5067
BclI T'GATC_A
Cuts at: 1548 2874 3540 1548
Size: 1326 666 4285
Fragments arranged by size:
4285 1326 666
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4888 136
Size: 107 122 71 631 605 3216 1525
Fragments arranged by size:
3216 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6272 6272
Size: 6277
BlpI GC'TnA_GC
Cuts at: 3694 3694
Size: 6277
Bme1580I G_kGCm'C
Cuts at: 1790 3178 4338 5494 1790
Size: 1388 1160 1156 2573
Fragments arranged by size:
2573 1388 1160 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6277
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2825 4008 4966
Size: 416 53 83 399 24 1408 1183 958
Cuts at: 4966 442
Size: 1753
Fragments arranged by size:
1753 1408 1183 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6277
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2448 2448
Size: 6277
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2022 2100 2301 4937 1239
Size: 783 78 201 2636 2579
Fragments arranged by size:
2636 2579 783 201 78
Bpu10I CC'TnA_GC
Cuts at: 3189 3189
Size: 6277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3370 4402 5267 5565 3370
Size: 1032 865 298 4082
Fragments arranged by size:
4082 1032 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3291 4940 882
Size: 617 1792 1649 2219
Fragments arranged by size:
2219 1792 1649 617
BsaAI yAC'GTr
Cuts at: 493 1204 4068 493
Size: 711 2864 2702
Fragments arranged by size:
2864 2702 711
BsaBI GATnn'nnATC
Cuts at: 6271 6271
Size: 6277
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4084 4466 5101 275
Size: 53 83 186 936 2551 382 635 1451
Fragments arranged by size:
2551 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2563 4710 5108 5382 5529 5940 2563
Size: 2147 398 274 147 411 2900
Fragments arranged by size:
2900 2147 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2527 2557 1178
Size: 30 165 30 1124 30 4898
Fragments arranged by size:
4898 1124 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2220 2424 2528 2531 2532 1186
Size: 1034 204 104 3 1 4931
Fragments arranged by size:
4931 1034 204 104 3 1
BseYI C'CCAG_C
Cuts at: 2984 5480 5976 2984
Size: 2496 496 3285
Fragments arranged by size:
3285 2496 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2598 1163
Size: 573 862 4842
Fragments arranged by size:
4842 862 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2564 3727 4488 5516 1540
Size: 275 186 563 1163 761 1028 2301
Fragments arranged by size:
2301 1163 1028 761 563 275 186
BsiHKAI G_wGCw'C
Cuts at: 2250 4338 4423 5494 2250
Size: 2088 85 1071 3033
Fragments arranged by size:
3033 2088 1071 85
BsmI GAATG_Cn'
Cuts at: 2482 3788 3881 2482
Size: 1306 93 4878
Fragments arranged by size:
4878 1306 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2250 2314 3178 3596 4338 4423 5494
Size: 399 460 64 864 418 742 85 1071
Cuts at: 5494 1391
Size: 2174
Fragments arranged by size:
2174 1071 864 742 460 418 399 85
64
BspEI T'CCGG_A
Cuts at: 5940 5940
Size: 6277
BspHI T'CATG_A
Cuts at: 4166 5896 4166
Size: 1730 4547
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3632 844
Size: 2788 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4164 1394
Size: 429 2341 3507
Fragments arranged by size:
3507 2341 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3567 4772 4946 65
Size: 1183 2319 1205 174 1396
Fragments arranged by size:
2319 1396 1205 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4921 5108 1700
Size: 110 3111 187 2869
Fragments arranged by size:
3111 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6277
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6277
BssSI C'ACGA_G
Cuts at: 4337 5349 4337
Size: 1012 5265
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2477 3038 794
Size: 1683 561 4033
Fragments arranged by size:
4033 1683 561
BstBI TT'CG_AA
Cuts at: 5081 5081
Size: 6277
BstEII G'GTnAC_C
Cuts at: 1184 2732 1184
Size: 1548 4729
BstXI CCAn_nnnn'nTGG
Cuts at: 2307 2307
Size: 6277
BstYI r'GATC_y
Cuts at: 1972 3143 3607 3974 4359 4376 5828 5914 5926
Size: 1171 464 367 385 17 1452 86 12
Cuts at: 5926 6272 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1171 464 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2364 2364
Size: 6277
BtgI C'CryG_G
Cuts at: 513 1065 3210 513
Size: 552 2145 3580
Fragments arranged by size:
3580 2145 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5538
Fragments arranged by size:
5538 683 56
BtsI GCAGTG_nn'
Cuts at: 2796 3793 4587 4607 2796
Size: 997 794 20 4466
Fragments arranged by size:
4466 997 794 20
ClaI AT'CG_AT
Cuts at: 1554 3439 1554
Size: 1885 4392
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6242
DraI TTT'AAA
Cuts at: 3581 3581
Size: 6277
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5284 809
Size: 507 3968 1802
Fragments arranged by size:
3968 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2216 3510 3724
Size: 54 1425 50 275 18 386 1294 214
Cuts at: 3724 4613 6242 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1294 889 386 275 214 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3724 1537
Size: 275 1912 4090
Fragments arranged by size:
4090 1912 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2133 2526 2688 3560 4207 1353
Size: 780 393 162 872 647 3423
Fragments arranged by size:
3423 872 780 647 393 162
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4864 5238 5384 387
Size: 1064 138 3275 374 146 1280
Fragments arranged by size:
3275 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2022 2100 2301 3377 4340 4937 5724
Size: 393 390 78 201 1076 963 597 787
Cuts at: 5724 5992 1239
Size: 268 1524
Fragments arranged by size:
1524 1076 963 787 597 393 390 268
201 78
EcoO109I rG'GnC_Cy
Cuts at: 3354 3354
Size: 6277
EcoRI G'AATT_C
Cuts at: 2285 3586 3964 2285
Size: 1301 378 4598
Fragments arranged by size:
4598 1301 378
FspI TGC'GCA
Cuts at: 4783 4783
Size: 6277
HaeII r_GCGC'y
Cuts at: 1678 5424 1678
Size: 3746 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2081 2113 2527 2559 3269
Size: 32 135 32 509 32 414 32 710
Cuts at: 3269 3301 4918 4950 4992 5024 1373
Size: 32 1617 32 42 32 2626
Fragments arranged by size:
2626 1617 710 509 414 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2926 3277 3621 3867 669
Size: 988 281 988 351 344 246 3079
Fragments arranged by size:
3079 988 988 351 344 281 246
HindIII A'AGCT_T
Cuts at: 748 3258 748
Size: 2510 3767
HpaI GTT'AAC
Cuts at: 2926 3867 2926
Size: 941 5336
KpnI G_GTAC'C
Cuts at: 3602 3602
Size: 6277
MfeI C'AATT_G
Cuts at: 3876 3876
Size: 6277
MluI A'CGCG_T
Cuts at: 6153 6153
Size: 6277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2424 3196 5391 5575 1305
Size: 1119 772 2195 184 2007
Fragments arranged by size:
2195 2007 1119 772 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3512 10
Size: 54 1425 343 1680 2775
Fragments arranged by size:
2775 1680 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2294 2637 3002 4235 4594 4753 518
Size: 648 1128 343 365 1233 359 159 2042
Fragments arranged by size:
2042 1233 1128 648 365 359 343 159
MspA1I CmG'CkG
Cuts at: 1606 3693 4370 5518 5763 1606
Size: 2087 677 1148 245 2120
Fragments arranged by size:
2120 2087 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3210 513
Size: 552 2145 3580
Fragments arranged by size:
3580 2145 552
NdeI CA'TA_TG
Cuts at: 387 3281 387
Size: 2894 3383
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6277
NotI GC'GGCC_GC
Cuts at: 3724 3724
Size: 6277
NspI r_CATG'y
Cuts at: 1132 1229 2299 2636 3086 3635 5180 1132
Size: 97 1070 337 450 549 1545 2229
Fragments arranged by size:
2229 1545 1070 549 450 337 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6277
PciI A'CATG_T
Cuts at: 2632 5176 2632
Size: 2544 3733
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6277
PmeI GTTT'AAAC
Cuts at: 3581 3581
Size: 6277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4346 4378 5859 5891 4346
Size: 32 1481 32 4732
Fragments arranged by size:
4732 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6277
PsiI TTA'TAA
Cuts at: 3847 3847
Size: 6277
PstI C_TGCA'G
Cuts at: 830 3629 830
Size: 2799 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6277
SalI G'TCGA_C
Cuts at: 1936 3275 3619 1936
Size: 1339 344 4594
Fragments arranged by size:
4594 1339 344
SbfI CC_TGCA'GG
Cuts at: 3629 3629
Size: 6277
ScaI AGT'ACT
Cuts at: 4525 6237 4525
Size: 1712 4565
SexAI A'CCwGG_T
Cuts at: 2943 2943
Size: 6277
SfcI C'TryA_G
Cuts at: 826 1045 2321 3625 4760 5441 5632 826
Size: 219 1276 1304 1135 681 191 1471
Fragments arranged by size:
1471 1304 1276 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6277
SmaI CCC'GGG
Cuts at: 1872 3604 1872
Size: 1732 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3349 4381 5282 5544 820
Size: 197 926 1406 1032 901 262 1553
Fragments arranged by size:
1553 1406 1032 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4068 493
Size: 3575 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6277
SphI G_CATG'C
Cuts at: 1132 2299 3635 1132
Size: 1167 1336 3774
Fragments arranged by size:
3774 1336 1167
SspI AAT'ATT
Cuts at: 5 52 3420 4201 5
Size: 47 3368 781 2081
Fragments arranged by size:
3368 2081 781 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6277
StyI C'CwwG_G
Cuts at: 513 1065 2481 3210 3716 513
Size: 552 1416 729 506 3074
Fragments arranged by size:
3074 1416 729 552 506
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4304 4321 4474 4659 5104 325
Size: 1255 2724 17 153 185 445 1498
Fragments arranged by size:
2724 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3124 4523 6235
Size: 274 80 33 51 1443 1147 1399 1712
Cuts at: 6235 96
Size: 138
Fragments arranged by size:
1712 1443 1399 1147 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6237 6269 6237
Size: 32 6245
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6277
XbaI T'CTAG_A
Cuts at: 3613 3613
Size: 6277
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6277
XmaI C'CCGG_G
Cuts at: 1870 3602 1870
Size: 1732 4545
XmnI GAAnn'nnTTC
Cuts at: 4406 4406
Size: 6277
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4085 276
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI XcmI