Restriction map for
pFN21AB5892
(Circular) (Six-base) MAPSORT of: pFN21AB5892.seq Check: 9700 from: 1 to: 6277
pFN21AB5892 6277 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4087 278
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2079 3620 1937
Size: 142 1541 4594
Fragments arranged by size:
4594 1541 142
Acc65I G'GTAC_C
Cuts at: 3598 3598
Size: 6277
AclI AA'CG_TT
Cuts at: 1563 4404 4777 1563
Size: 2841 373 3063
Fragments arranged by size:
3063 2841 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2323 2681 2733 2871 4340 5724 5992 1632
Size: 691 358 52 138 1469 1384 268 1917
Fragments arranged by size:
1917 1469 1384 691 358 268 138 52
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6080
AflIII A'CryG_T
Cuts at: 1203 2396 5176 6153 1203
Size: 1193 2780 977 1327
Fragments arranged by size:
2780 1327 1193 977
AgeI A'CCGG_T
Cuts at: 5108 5108
Size: 6277
AhdI GACnn_n'nnGTC
Cuts at: 3175 5006 3175
Size: 1831 4446
AleI CACnn'nnGTG
Cuts at: 1166 3572 1166
Size: 2406 3871
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6277
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3072 3104 3072
Size: 32 6245
AlwNI CAG_nnn'CTG
Cuts at: 2908 3187 4020 5592 2908
Size: 279 833 1572 3593
Fragments arranged by size:
3593 1572 833 279
ApaLI G'TGCA_C
Cuts at: 3303 4334 5490 3303
Size: 1031 1156 4090
Fragments arranged by size:
4090 1156 1031
ApoI r'AATT_y
Cuts at: 1493 2214 2292 2389 3487 3586 3812 3964 6073
Size: 721 78 97 1098 99 226 152 2109
Cuts at: 6073 1493
Size: 1697
Fragments arranged by size:
2109 1697 1098 721 226 152 99 97
78
AseI AT'TA_AT
Cuts at: 160 1032 4831 160
Size: 872 3799 1606
Fragments arranged by size:
3799 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
AvaI C'yCGr_G
Cuts at: 1870 1943 2087 2159 3602 1870
Size: 73 144 72 1443 4545
Fragments arranged by size:
4545 1443 144 73 72
BamHI G'GATC_C
Cuts at: 3607 3974 3607
Size: 367 5910
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3598 5053 618
Size: 325 217 627 1811 1455 1842
Fragments arranged by size:
1842 1811 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2590 3596 1391
Size: 1199 1006 4072
Fragments arranged by size:
4072 1199 1006
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2533 3414 928
Size: 971 634 881 3791
Fragments arranged by size:
3791 971 881 634
BbvCI CC'TCA_GC
Cuts at: 3255 3255
Size: 6277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4468 4502 1903
Size: 34 2531 34 3678
Fragments arranged by size:
3678 2531 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3045 4169 5379 3045
Size: 1124 1210 3943
Fragments arranged by size:
3943 1210 1124
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6277
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4888 136
Size: 107 122 71 631 605 3216 1525
Fragments arranged by size:
3216 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2094 2605 6272 2094
Size: 511 3667 2099
Fragments arranged by size:
3667 2099 511
BlpI GC'TnA_GC
Cuts at: 3694 3694
Size: 6277
Bme1580I G_kGCm'C
Cuts at: 1790 3307 4338 5494 1790
Size: 1517 1031 1156 2573
Fragments arranged by size:
2573 1517 1156 1031
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6277
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4008 4966 442
Size: 416 53 83 399 24 2591 958 1753
Fragments arranged by size:
2591 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6277
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 2214 3329 4937 1239
Size: 899 76 1115 1608 2579
Fragments arranged by size:
2579 1608 1115 899 76
Bpu10I CC'TnA_GC
Cuts at: 3255 3255
Size: 6277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4402 5267 5565 4402
Size: 865 298 5114
Fragments arranged by size:
5114 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4940 882
Size: 617 3441 2219
Fragments arranged by size:
3441 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3032 4068 493
Size: 711 1828 1036 2702
Fragments arranged by size:
2702 1828 1036 711
BsaBI GATnn'nnATC
Cuts at: 2622 6271 2622
Size: 3649 2628
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2136 4084 4466 5101
Size: 53 83 186 936 603 1948 382 635
Cuts at: 5101 275
Size: 1451
Fragments arranged by size:
1948 1451 936 635 603 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2779 4710 5108 5382 5529 5940 2779
Size: 1931 398 274 147 411 3116
Fragments arranged by size:
3116 1931 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6052
Fragments arranged by size:
6052 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 2135 1186
Size: 919 30 5328
Fragments arranged by size:
5328 919 30
BseYI C'CCAG_C
Cuts at: 2517 3180 5480 5976 2517
Size: 663 2300 496 2818
Fragments arranged by size:
2818 2300 663 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5704
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3727 4488 5516 1540
Size: 275 186 1726 761 1028 2301
Fragments arranged by size:
2301 1726 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3307 4338 4423 5494 3307
Size: 1031 85 1071 4090
Fragments arranged by size:
4090 1071 1031 85
BsmI GAATG_Cn'
Cuts at: 2962 3511 3788 3881 2962
Size: 549 277 93 5358
Fragments arranged by size:
5358 549 277 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3410 3410
Size: 6277
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2590 3307 3596 4338 4423 5494 1391
Size: 399 800 717 289 742 85 1071 2174
Fragments arranged by size:
2174 1071 800 742 717 399 289 85
BspEI T'CCGG_A
Cuts at: 2779 5940 2779
Size: 3161 3116
BspHI T'CATG_A
Cuts at: 4166 5896 4166
Size: 1730 4547
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2317 2676 3064 3632 844
Size: 1473 359 388 568 3489
Fragments arranged by size:
3489 1473 568 388 359
BsrBI CCG'CTC
Cuts at: 1394 1823 4164 1394
Size: 429 2341 3507
Fragments arranged by size:
3507 2341 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4772 4946 65
Size: 1183 3524 174 1396
Fragments arranged by size:
3524 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4921 5108 1700
Size: 110 3111 187 2869
Fragments arranged by size:
3111 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6277
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6277
BssSI C'ACGA_G
Cuts at: 4337 5349 4337
Size: 1012 5265
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6277
BstBI TT'CG_AA
Cuts at: 5081 5081
Size: 6277
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6277
BstXI CCAn_nnnn'nTGG
Cuts at: 3574 3574
Size: 6277
BstYI r'GATC_y
Cuts at: 1972 2094 2605 3607 3974 4359 4376 5828 5914
Size: 122 511 1002 367 385 17 1452 86
Cuts at: 5914 5926 6272 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1002 511 385 367 346 122
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2643 2643
Size: 6277
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5725
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5538
Fragments arranged by size:
5538 683 56
BtsI GCAGTG_nn'
Cuts at: 2011 2065 2321 3793 4587 4607 2011
Size: 54 256 1472 794 20 3681
Fragments arranged by size:
3681 1472 794 256 54 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6277
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6242
DraI TTT'AAA
Cuts at: 3581 3581
Size: 6277
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5284 809
Size: 507 3968 1802
Fragments arranged by size:
3968 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3724 4613 6242
Size: 54 1425 50 275 18 1894 889 1629
Cuts at: 6242 8
Size: 43
Fragments arranged by size:
1894 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3724 1537
Size: 275 1912 4090
Fragments arranged by size:
4090 1912 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4207 1353
Size: 2854 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4864 5238 5384 387
Size: 1064 138 3275 374 146 1280
Fragments arranged by size:
3275 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2214 2323 2681 2733 2871 3329
Size: 393 506 76 109 358 52 138 458
Cuts at: 3329 4340 4937 5724 5992 1239
Size: 1011 597 787 268 1524
Fragments arranged by size:
1524 1011 787 597 506 458 393 358
268 138 109 76 52
EcoRI G'AATT_C
Cuts at: 3586 3964 3586
Size: 378 5899
FspI TGC'GCA
Cuts at: 4783 4783
Size: 6277
HaeII r_GCGC'y
Cuts at: 1678 2065 5424 1678
Size: 387 3359 2531
Fragments arranged by size:
3359 2531 387
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2470 2502 3161 3193 4918
Size: 32 135 32 898 32 659 32 1725
Cuts at: 4918 4950 4992 5024 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1725 898 659 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3361 3621 3867 669
Size: 988 281 1423 260 246 3079
Fragments arranged by size:
3079 1423 988 281 260 246
HindIII A'AGCT_T
Cuts at: 748 2192 2371 748
Size: 1444 179 4654
Fragments arranged by size:
4654 1444 179
HpaI GTT'AAC
Cuts at: 3867 3867
Size: 6277
KpnI G_GTAC'C
Cuts at: 3602 3602
Size: 6277
MfeI C'AATT_G
Cuts at: 3876 3876
Size: 6277
MluI A'CGCG_T
Cuts at: 6153 6153
Size: 6277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2563 2595 2679 5391 5575 1305
Size: 1258 32 84 2712 184 2007
Fragments arranged by size:
2712 2007 1258 184 84 32
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4455
Fragments arranged by size:
4455 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3572 4235 4594 4753 518
Size: 648 2406 663 359 159 2042
Fragments arranged by size:
2406 2042 663 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2221 2254 2343 2521 3259 3693 4370 5518
Size: 615 33 89 178 738 434 677 1148
Cuts at: 5518 5763 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 738 677 615 434 245 178
89 33
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5725
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6277
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6277
NotI GC'GGCC_GC
Cuts at: 3724 3724
Size: 6277
NspI r_CATG'y
Cuts at: 1132 1229 2400 3635 5180 1132
Size: 97 1171 1235 1545 2229
Fragments arranged by size:
2229 1545 1235 1171 97
PasI CC'CwG_GG
Cuts at: 1348 3452 1348
Size: 2104 4173
PciI A'CATG_T
Cuts at: 2396 5176 2396
Size: 2780 3497
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6277
PfoI T'CCnGG_A
Cuts at: 3090 3090
Size: 6277
PmeI GTTT'AAAC
Cuts at: 3581 3581
Size: 6277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4346 4378 5859 5891 4346
Size: 32 1481 32 4732
Fragments arranged by size:
4732 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6277
PsiI TTA'TAA
Cuts at: 2457 3847 2457
Size: 1390 4887
PstI C_TGCA'G
Cuts at: 830 2328 3011 3061 3629 830
Size: 1498 683 50 568 3478
Fragments arranged by size:
3478 1498 683 568 50
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6277
PvuII CAG'CTG
Cuts at: 2254 2521 3259 2254
Size: 267 738 5272
Fragments arranged by size:
5272 738 267
SalI G'TCGA_C
Cuts at: 1936 3619 1936
Size: 1683 4594
SbfI CC_TGCA'GG
Cuts at: 3629 3629
Size: 6277
ScaI AGT'ACT
Cuts at: 4525 6237 4525
Size: 1712 4565
SfcI C'TryA_G
Cuts at: 826 1045 2324 2423 3007 3057 3625 4760 5441
Size: 219 1279 99 584 50 568 1135 681
Cuts at: 5441 5632 826
Size: 191 1471
Fragments arranged by size:
1471 1279 1135 681 584 568 219 191
99 50
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6277
SmaI CCC'GGG
Cuts at: 1872 3604 1872
Size: 1732 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4381 5282 5544 820
Size: 197 926 2438 901 262 1553
Fragments arranged by size:
2438 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4068 493
Size: 3575 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6277
SphI G_CATG'C
Cuts at: 1132 3635 1132
Size: 2503 3774
SspI AAT'ATT
Cuts at: 5 52 2443 4201 5
Size: 47 2391 1758 2081
Fragments arranged by size:
2391 2081 1758 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6277
StyI C'CwwG_G
Cuts at: 513 1065 3716 513
Size: 552 2651 3074
Fragments arranged by size:
3074 2651 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3465 3573 4304 4321 4474 4659 5104
Size: 1255 1885 108 731 17 153 185 445
Cuts at: 5104 325
Size: 1498
Fragments arranged by size:
1885 1498 1255 731 445 185 153 108
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4523 6235 96
Size: 274 80 33 51 1443 2546 1712 138
Fragments arranged by size:
2546 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3337 3369 6237 6269 3337
Size: 32 2868 32 3345
Fragments arranged by size:
3345 2868 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6277
XbaI T'CTAG_A
Cuts at: 3613 3613
Size: 6277
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6277
XmaI C'CCGG_G
Cuts at: 1870 3602 1870
Size: 1732 4545
XmnI GAAnn'nnTTC
Cuts at: 2373 2706 2775 4406 2373
Size: 333 69 1631 4244
Fragments arranged by size:
4244 1631 333 69
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4085 276
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI