Restriction map for
pFN21AB5891
(Circular) (Six-base) MAPSORT of: pFN21AB5891.seq Check: 5452 from: 1 to: 6199
pFN21AB5891 6199 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2971 2971
Size: 6199
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4009 278
Size: 53 83 186 936 2473 2468
Fragments arranged by size:
2473 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2220 3542 1937
Size: 283 1322 4594
Fragments arranged by size:
4594 1322 283
Acc65I G'GTAC_C
Cuts at: 3520 3520
Size: 6199
AclI AA'CG_TT
Cuts at: 1563 4326 4699 1563
Size: 2763 373 3063
Fragments arranged by size:
3063 2763 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2287 4262 5646 5914 1632
Size: 655 1975 1384 268 1917
Fragments arranged by size:
1975 1917 1384 655 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6002
AflIII A'CryG_T
Cuts at: 1203 2597 5098 6075 1203
Size: 1394 2501 977 1327
Fragments arranged by size:
2501 1394 1327 977
AgeI A'CCGG_T
Cuts at: 5030 5030
Size: 6199
AhdI GACnn_n'nnGTC
Cuts at: 3490 4928 3490
Size: 1438 4761
AleI CACnn'nnGTG
Cuts at: 1166 2687 1166
Size: 1521 4678
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6199
AlwNI CAG_nnn'CTG
Cuts at: 2360 2765 3122 3942 5514 2360
Size: 405 357 820 1572 3045
Fragments arranged by size:
3045 1572 820 405 357
ApaI G_GGCC'C
Cuts at: 3210 3210
Size: 6199
ApaLI G'TGCA_C
Cuts at: 4256 5412 4256
Size: 1156 5043
ApoI r'AATT_y
Cuts at: 1493 2447 2636 2822 3473 3508 3734 3886 5995
Size: 954 189 186 651 35 226 152 2109
Cuts at: 5995 1493
Size: 1697
Fragments arranged by size:
2109 1697 954 651 226 189 186 152
35
AseI AT'TA_AT
Cuts at: 160 1032 4753 160
Size: 872 3721 1606
Fragments arranged by size:
3721 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6199
AvaI C'yCGr_G
Cuts at: 1870 1943 2193 2945 3524 1870
Size: 73 250 752 579 4545
Fragments arranged by size:
4545 752 579 250 73
BamHI G'GATC_C
Cuts at: 2251 3529 3896 2251
Size: 1278 367 4554
Fragments arranged by size:
4554 1278 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2037 3520 4975 618
Size: 325 217 627 250 1483 1455 1842
Fragments arranged by size:
1842 1483 1455 627 325 250 217
BanII G_rGCy'C
Cuts at: 1391 2166 3210 3518 1391
Size: 775 1044 308 4072
Fragments arranged by size:
4072 1044 775 308
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5228
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4390 4424 1903
Size: 34 50 34 2369 34 3678
Fragments arranged by size:
3678 2369 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2685 4091 5301 2685
Size: 1406 1210 3583
Fragments arranged by size:
3583 1406 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6199
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4810 136
Size: 107 122 71 631 605 3138 1525
Fragments arranged by size:
3138 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6194 6194
Size: 6199
BlpI GC'TnA_GC
Cuts at: 3616 3616
Size: 6199
Bme1580I G_kGCm'C
Cuts at: 1790 3210 4260 5416 1790
Size: 1420 1050 1156 2573
Fragments arranged by size:
2573 1420 1156 1050
BmgBI CAC'GTC
Cuts at: 1318 3409 1318
Size: 2091 4108
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2348 2828 3930
Size: 416 53 83 399 24 931 480 1102
Cuts at: 3930 4888 442
Size: 958 1753
Fragments arranged by size:
1753 1102 958 931 480 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3018 4859 1239
Size: 1779 1841 2579
Fragments arranged by size:
2579 1841 1779
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4324 5189 5487 4324
Size: 865 298 5036
Fragments arranged by size:
5036 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3036 4862 882
Size: 617 1537 1826 2219
Fragments arranged by size:
2219 1826 1537 617
BsaAI yAC'GTr
Cuts at: 493 1204 2585 2600 3990 493
Size: 711 1381 15 1390 2702
Fragments arranged by size:
2702 1390 1381 711 15
BsaBI GATnn'nnATC
Cuts at: 2996 6193 2996
Size: 3197 3002
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3041 4006 4388 5023
Size: 53 83 186 936 1508 965 382 635
Cuts at: 5023 275
Size: 1451
Fragments arranged by size:
1508 1451 965 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4632 5030 5304 5451 5862 4632
Size: 398 274 147 411 4969
Fragments arranged by size:
4969 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5974
Fragments arranged by size:
5974 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2382 2799 2904 2907 3093 3096 3168 3171
Size: 1196 417 105 3 186 3 72 3
Cuts at: 3171 3180 3183 3186 3189 3204 3380 3457 1186
Size: 9 3 3 3 15 176 77 3928
Fragments arranged by size:
3928 1196 417 186 176 105 77 72
15 9 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2122 2659 3156 5402 5898 2122
Size: 537 497 2246 496 2423
Fragments arranged by size:
2423 2246 537 497 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3252 1163
Size: 573 1516 4110
Fragments arranged by size:
4110 1516 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2224 3649 4410 5438 1540
Size: 275 186 223 1425 761 1028 2301
Fragments arranged by size:
2301 1425 1028 761 275 223 186
BsiHKAI G_wGCw'C
Cuts at: 2166 3175 4260 4345 5416 2166
Size: 1009 1085 85 1071 2949
Fragments arranged by size:
2949 1085 1071 1009 85
BsmI GAATG_Cn'
Cuts at: 2087 3710 3803 2087
Size: 1623 93 4483
Fragments arranged by size:
4483 1623 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2166 3175 3210 3518 4260 4345 5416
Size: 399 376 1009 35 308 742 85 1071
Cuts at: 5416 1391
Size: 2174
Fragments arranged by size:
2174 1071 1009 742 399 376 308 85
35
BspEI T'CCGG_A
Cuts at: 5862 5862
Size: 6199
BspHI T'CATG_A
Cuts at: 2517 4088 5818 2517
Size: 1571 1730 2898
Fragments arranged by size:
2898 1730 1571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2971 3330 3554 844
Size: 2127 359 224 3489
Fragments arranged by size:
3489 2127 359 224
BsrBI CCG'CTC
Cuts at: 1394 1823 4086 1394
Size: 429 2263 3507
Fragments arranged by size:
3507 2263 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3409 3432 4694 4868 65
Size: 1183 2161 23 1262 174 1396
Fragments arranged by size:
2161 1396 1262 1183 174 23
BsrFI r'CCGG_y
Cuts at: 1700 1810 4843 5030 1700
Size: 110 3033 187 2869
Fragments arranged by size:
3033 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6199
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6199
BssSI C'ACGA_G
Cuts at: 4259 5271 4259
Size: 1012 5187
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6199
BstBI TT'CG_AA
Cuts at: 5003 5003
Size: 6199
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6199
BstYI r'GATC_y
Cuts at: 1972 2251 2852 2876 3529 3896 4281 4298 5750
Size: 279 601 24 653 367 385 17 1452
Cuts at: 5750 5836 5848 6194 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 653 601 385 367 346 279
86 24 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5647
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3321 487
Size: 683 56 2095 3365
Fragments arranged by size:
3365 2095 683 56
BtsI GCAGTG_nn'
Cuts at: 2273 3715 4509 4529 2273
Size: 1442 794 20 3943
Fragments arranged by size:
3943 1442 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6199
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2667 2702 3132 3167 529
Size: 35 2103 35 430 35 3561
Fragments arranged by size:
3561 2103 430 35 35 35
DraI TTT'AAA
Cuts at: 3503 3503
Size: 6199
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5206 809
Size: 507 3890 1802
Fragments arranged by size:
3890 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3238 3411 3646
Size: 54 1425 50 275 18 1408 173 235
Cuts at: 3646 4535 6164 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1408 889 275 235 173 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3646 1537
Size: 275 1834 4090
Fragments arranged by size:
4090 1834 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2522 2601 4129 1353
Size: 1169 79 1528 3423
Fragments arranged by size:
3423 1528 1169 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2733 2961 2970 4786 5160 5306
Size: 1064 138 1144 228 9 1816 374 146
Cuts at: 5306 387
Size: 1280
Fragments arranged by size:
1816 1280 1144 1064 374 228 146 138
9
EcoICRI GAG'CTC
Cuts at: 2164 2164
Size: 6199
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2287 3018 4262 4859 5646 5914 1239
Size: 393 655 731 1244 597 787 268 1524
Fragments arranged by size:
1524 1244 787 731 655 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2919 2919
Size: 6199
EcoO109I rG'GnC_Cy
Cuts at: 2936 3206 3207 2936
Size: 270 1 5928
Fragments arranged by size:
5928 270 1
EcoRI G'AATT_C
Cuts at: 2822 3508 3886 2822
Size: 686 378 5135
Fragments arranged by size:
5135 686 378
EcoRV GAT'ATC
Cuts at: 2515 2515
Size: 6199
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3307 3307
Size: 6199
FspI TGC'GCA
Cuts at: 3356 4705 3356
Size: 1349 4850
HaeII r_GCGC'y
Cuts at: 1678 5346 1678
Size: 3668 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2330 2362 3452 3476 3484
Size: 32 135 32 758 32 1090 24 8
Cuts at: 3484 3508 4840 4872 4914 4946 1373
Size: 24 1332 32 42 32 2626
Fragments arranged by size:
2626 1332 1090 758 135 42 32 32
32 32 32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2221 3543 3789 669
Size: 988 281 283 1322 246 3079
Fragments arranged by size:
3079 1322 988 283 281 246
HindIII A'AGCT_T
Cuts at: 748 2330 748
Size: 1582 4617
HpaI GTT'AAC
Cuts at: 3789 3789
Size: 6199
KpnI G_GTAC'C
Cuts at: 3524 3524
Size: 6199
MfeI C'AATT_G
Cuts at: 3798 3798
Size: 6199
MluI A'CGCG_T
Cuts at: 6075 6075
Size: 6199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2140 5313 5497 1305
Size: 835 3173 184 2007
Fragments arranged by size:
3173 2007 835 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3240 3413 10
Size: 54 1425 343 1408 173 2796
Fragments arranged by size:
2796 1425 1408 343 173 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2687 4157 4516 4675 518
Size: 648 1521 1470 359 159 2042
Fragments arranged by size:
2042 1521 1470 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2122 2248 3122 3352 3373 3615 4292 5440
Size: 516 126 874 230 21 242 677 1148
Cuts at: 5440 5685 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 874 677 516 245 242 230
126 21
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5647
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6199
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6199
NotI GC'GGCC_GC
Cuts at: 3646 3646
Size: 6199
NspI r_CATG'y
Cuts at: 1132 1229 3557 5102 1132
Size: 97 2328 1545 2229
Fragments arranged by size:
2328 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6199
PciI A'CATG_T
Cuts at: 5098 5098
Size: 6199
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6199
PmeI GTTT'AAAC
Cuts at: 3503 3503
Size: 6199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4268 4300 5781 5813 4268
Size: 32 1481 32 4654
Fragments arranged by size:
4654 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6199
PsiI TTA'TAA
Cuts at: 3769 3769
Size: 6199
PspOMI G'GGCC_C
Cuts at: 3206 3206
Size: 6199
PstI C_TGCA'G
Cuts at: 830 2502 3551 830
Size: 1672 1049 3478
Fragments arranged by size:
3478 1672 1049
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6199
PvuII CAG'CTG
Cuts at: 2122 2248 3122 3352 2122
Size: 126 874 230 4969
Fragments arranged by size:
4969 874 230 126
SacI G_AGCT'C
Cuts at: 2166 2166
Size: 6199
SalI G'TCGA_C
Cuts at: 1936 2219 3541 1936
Size: 283 1322 4594
Fragments arranged by size:
4594 1322 283
SbfI CC_TGCA'GG
Cuts at: 3551 3551
Size: 6199
ScaI AGT'ACT
Cuts at: 4447 6159 4447
Size: 1712 4487
SfcI C'TryA_G
Cuts at: 826 1045 2228 2498 3018 3547 4682 5363 5554
Size: 219 1183 270 520 529 1135 681 191
Cuts at: 5554 826
Size: 1471
Fragments arranged by size:
1471 1183 1135 681 529 520 270 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6199
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6199
SmaI CCC'GGG
Cuts at: 1872 2947 3526 1872
Size: 1075 579 4545
Fragments arranged by size:
4545 1075 579
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4303 5204 5466 820
Size: 197 926 2360 901 262 1553
Fragments arranged by size:
2360 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3990 493
Size: 3497 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6199
SphI G_CATG'C
Cuts at: 1132 3557 1132
Size: 2425 3774
SspI AAT'ATT
Cuts at: 5 52 4123 5
Size: 47 4071 2081
Fragments arranged by size:
4071 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6199
StyI C'CwwG_G
Cuts at: 513 1065 2033 2256 2508 3638 513
Size: 552 968 223 252 1130 3074
Fragments arranged by size:
3074 1130 968 552 252 223
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2238 2695 4226 4243 4396 4581 5026
Size: 1255 658 457 1531 17 153 185 445
Cuts at: 5026 325
Size: 1498
Fragments arranged by size:
1531 1498 1255 658 457 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3444 4445 6157
Size: 274 80 33 51 1443 1467 1001 1712
Cuts at: 6157 96
Size: 138
Fragments arranged by size:
1712 1467 1443 1001 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6159 6191 6159
Size: 32 6167
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6199
XbaI T'CTAG_A
Cuts at: 3535 3535
Size: 6199
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2098 2656 2852 2098
Size: 558 196 5445
Fragments arranged by size:
5445 558 196
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6199
XmaI C'CCGG_G
Cuts at: 1870 2945 3524 1870
Size: 1075 579 4545
Fragments arranged by size:
4545 1075 579
XmnI GAAnn'nnTTC
Cuts at: 4328 4328
Size: 6199
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4007 276
Size: 53 83 186 936 2473 2468
Fragments arranged by size:
2473 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI