Restriction map for
pFN21AB5888
(Circular) (Six-base) MAPSORT of: pFN21AB5888.seq Check: 345 from: 1 to: 6268
pFN21AB5888 6268 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4078 278
Size: 53 83 186 936 2542 2468
Fragments arranged by size:
2542 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2784 3611 1937
Size: 847 827 4594
Fragments arranged by size:
4594 847 827
Acc65I G'GTAC_C
Cuts at: 3515 3589 3515
Size: 74 6194
AclI AA'CG_TT
Cuts at: 1563 4395 4768 1563
Size: 2832 373 3063
Fragments arranged by size:
3063 2832 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3519 4331 5715 5983 1632
Size: 1887 812 1384 268 1917
Fragments arranged by size:
1917 1887 1384 812 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6071
AflIII A'CryG_T
Cuts at: 1203 5167 6144 1203
Size: 3964 977 1327
Fragments arranged by size:
3964 1327 977
AgeI A'CCGG_T
Cuts at: 5099 5099
Size: 6268
AhdI GACnn_n'nnGTC
Cuts at: 4997 4997
Size: 6268
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6268
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2490 1184
Size: 1306 4962
AlwNI CAG_nnn'CTG
Cuts at: 4011 5583 4011
Size: 1572 4696
ApaLI G'TGCA_C
Cuts at: 4325 5481 4325
Size: 1156 5112
ApoI r'AATT_y
Cuts at: 1493 3557 3577 3803 3955 6064 1493
Size: 2064 20 226 152 2109 1697
Fragments arranged by size:
2109 2064 1697 226 152 20
AseI AT'TA_AT
Cuts at: 160 1032 3110 4822 160
Size: 872 2078 1712 1606
Fragments arranged by size:
2078 1712 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6268
AvaI C'yCGr_G
Cuts at: 1870 1943 3593 1870
Size: 73 1650 4545
Fragments arranged by size:
4545 1650 73
BamHI G'GATC_C
Cuts at: 2698 3598 3965 2698
Size: 900 367 5001
Fragments arranged by size:
5001 900 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2945 3515 3589 5044 618
Size: 325 217 627 1158 570 74 1455 1842
Fragments arranged by size:
1842 1455 1158 627 570 325 217 74
BanII G_rGCy'C
Cuts at: 1391 2800 3587 1391
Size: 1409 787 4072
Fragments arranged by size:
4072 1409 787
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2089 3423 928
Size: 971 190 1334 3773
Fragments arranged by size:
3773 1334 971 190
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4459 4493 1903
Size: 34 2522 34 3678
Fragments arranged by size:
3678 2522 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4160 5370 4160
Size: 1210 5058
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6268
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4879 136
Size: 107 122 71 631 605 3207 1525
Fragments arranged by size:
3207 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6263 6263
Size: 6268
BlpI GC'TnA_GC
Cuts at: 3685 3685
Size: 6268
Bme1580I G_kGCm'C
Cuts at: 1790 2865 2877 4329 5485 1790
Size: 1075 12 1452 1156 2573
Fragments arranged by size:
2573 1452 1156 1075 12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6268
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2252 2606 3999
Size: 416 53 83 399 24 835 354 1393
Cuts at: 3999 4957 442
Size: 958 1753
Fragments arranged by size:
1753 1393 958 835 416 399 354 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4928 1239
Size: 3689 2579
Bpu10I CC'TnA_GC
Cuts at: 3138 3138
Size: 6268
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2439 3264 4393 5258 5556 2439
Size: 825 1129 865 298 3151
Fragments arranged by size:
3151 1129 865 825 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4931 882
Size: 617 3432 2219
Fragments arranged by size:
3432 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4059 493
Size: 711 2855 2702
Fragments arranged by size:
2855 2702 711
BsaBI GATnn'nnATC
Cuts at: 2385 3480 6262 2385
Size: 1095 2782 2391
Fragments arranged by size:
2782 2391 1095
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4075 4457 5092 275
Size: 53 83 186 936 2542 382 635 1451
Fragments arranged by size:
2542 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4701 5099 5373 5520 5931 4701
Size: 398 274 147 411 5038
Fragments arranged by size:
5038 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6043
Fragments arranged by size:
6043 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6268
BseYI C'CCAG_C
Cuts at: 2018 5471 5967 2018
Size: 3453 496 2319
Fragments arranged by size:
3453 2319 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2430 2486 1163
Size: 573 694 56 4945
Fragments arranged by size:
4945 694 573 56
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3718 4479 5507 1540
Size: 275 186 1717 761 1028 2301
Fragments arranged by size:
2301 1717 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4329 4414 5485 4329
Size: 85 1071 5112
Fragments arranged by size:
5112 1071 85
BsmI GAATG_Cn'
Cuts at: 2245 3779 3872 2245
Size: 1534 93 4641
Fragments arranged by size:
4641 1534 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2800 2865 2877 3587 4329 4414 5485
Size: 399 1010 65 12 710 742 85 1071
Cuts at: 5485 1391
Size: 2174
Fragments arranged by size:
2174 1071 1010 742 710 399 85 65
12
BspEI T'CCGG_A
Cuts at: 5931 5931
Size: 6268
BspHI T'CATG_A
Cuts at: 4157 5887 4157
Size: 1730 4538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3623 844
Size: 2779 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4155 1394
Size: 429 2332 3507
Fragments arranged by size:
3507 2332 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4763 4937 65
Size: 1183 3515 174 1396
Fragments arranged by size:
3515 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3329 4912 5099 1700
Size: 110 1519 1583 187 2869
Fragments arranged by size:
2869 1583 1519 187 110
BsrGI T'GTAC_A
Cuts at: 96 3226 96
Size: 3130 3138
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6268
BssSI C'ACGA_G
Cuts at: 4328 5340 4328
Size: 1012 5256
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6268
BstBI TT'CG_AA
Cuts at: 5072 5072
Size: 6268
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6268
BstYI r'GATC_y
Cuts at: 1972 2698 3481 3598 3965 4350 4367 5819 5905
Size: 726 783 117 367 385 17 1452 86
Cuts at: 5905 5917 6263 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 783 726 385 367 346 117
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4777
Fragments arranged by size:
4777 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2079 487
Size: 683 56 853 4676
Fragments arranged by size:
4676 853 683 56
BtsI GCAGTG_nn'
Cuts at: 3048 3784 4578 4598 3048
Size: 736 794 20 4718
Fragments arranged by size:
4718 794 736 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6268
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6233
DraI TTT'AAA
Cuts at: 2203 3572 2203
Size: 1369 4899
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5275 809
Size: 507 3959 1802
Fragments arranged by size:
3959 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3327 3715
Size: 54 1425 50 275 18 177 1320 388
Cuts at: 3715 4604 6233 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1320 889 388 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3715 1537
Size: 275 1903 4090
Fragments arranged by size:
4090 1903 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2619 4198 1353
Size: 1266 1579 3423
Fragments arranged by size:
3423 1579 1266
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4855 5229 5375 387
Size: 1064 138 3266 374 146 1280
Fragments arranged by size:
3266 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3519 4331 4928 5715 5983 1239
Size: 393 1887 812 597 787 268 1524
Fragments arranged by size:
1887 1524 812 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3577 3955 3577
Size: 378 5890
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3163 3163
Size: 6268
FspI TGC'GCA
Cuts at: 4774 4774
Size: 6268
HaeII r_GCGC'y
Cuts at: 1678 5415 1678
Size: 3737 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2571 2603 2972 3004 3112
Size: 32 135 32 999 32 369 32 108
Cuts at: 3112 3144 4909 4941 4983 5015 1373
Size: 32 1765 32 42 32 2626
Fragments arranged by size:
2626 1765 999 369 135 108 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3612 3858 669
Size: 988 281 1674 246 3079
Fragments arranged by size:
3079 1674 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6268
HpaI GTT'AAC
Cuts at: 3858 3858
Size: 6268
KpnI G_GTAC'C
Cuts at: 3519 3593 3519
Size: 74 6194
MfeI C'AATT_G
Cuts at: 2267 2974 3867 2267
Size: 707 893 4668
Fragments arranged by size:
4668 893 707
MluI A'CGCG_T
Cuts at: 6144 6144
Size: 6268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3081 3557 5382 5566 1305
Size: 1776 476 1825 184 2007
Fragments arranged by size:
2007 1825 1776 476 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4446
Fragments arranged by size:
4446 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2091 2517 3411 4226 4585 4744 518
Size: 648 925 426 894 815 359 159 2042
Fragments arranged by size:
2042 925 894 815 648 426 359 159
MspA1I CmG'CkG
Cuts at: 1606 3684 4361 5509 5754 1606
Size: 2078 677 1148 245 2120
Fragments arranged by size:
2120 2078 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4777
Fragments arranged by size:
4777 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6268
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6268
NotI GC'GGCC_GC
Cuts at: 3715 3715
Size: 6268
NspI r_CATG'y
Cuts at: 1132 1229 3626 5171 1132
Size: 97 2397 1545 2229
Fragments arranged by size:
2397 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6268
PciI A'CATG_T
Cuts at: 5167 5167
Size: 6268
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6268
PmeI GTTT'AAAC
Cuts at: 3572 3572
Size: 6268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4337 4369 5850 5882 4337
Size: 32 1481 32 4723
Fragments arranged by size:
4723 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6268
PsiI TTA'TAA
Cuts at: 3838 3838
Size: 6268
PstI C_TGCA'G
Cuts at: 830 2690 3620 830
Size: 1860 930 3478
Fragments arranged by size:
3478 1860 930
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6268
SalI G'TCGA_C
Cuts at: 1936 3610 1936
Size: 1674 4594
SbfI CC_TGCA'GG
Cuts at: 3620 3620
Size: 6268
ScaI AGT'ACT
Cuts at: 4516 6228 4516
Size: 1712 4556
SfcI C'TryA_G
Cuts at: 826 1045 2686 3616 4751 5432 5623 826
Size: 219 1641 930 1135 681 191 1471
Fragments arranged by size:
1641 1471 1135 930 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6268
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6268
SmaI CCC'GGG
Cuts at: 1872 3595 1872
Size: 1723 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2454 3279 4372 5273 5535 820
Size: 197 926 511 825 1093 901 262 1553
Fragments arranged by size:
1553 1093 926 901 825 511 262 197
SnaBI TAC'GTA
Cuts at: 493 4059 493
Size: 3566 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6268
SphI G_CATG'C
Cuts at: 1132 3626 1132
Size: 2494 3774
SspI AAT'ATT
Cuts at: 5 52 4192 5
Size: 47 4140 2081
Fragments arranged by size:
4140 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6268
StyI C'CwwG_G
Cuts at: 513 1065 2004 3707 513
Size: 552 939 1703 3074
Fragments arranged by size:
3074 1703 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3030 4295 4312 4465 4650 5095 325
Size: 1255 1450 1265 17 153 185 445 1498
Fragments arranged by size:
1498 1450 1265 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3226 4514 6226
Size: 274 80 33 51 1443 1249 1288 1712
Cuts at: 6226 96
Size: 138
Fragments arranged by size:
1712 1443 1288 1249 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3029 3061 6228 6260 3029
Size: 32 3167 32 3037
Fragments arranged by size:
3167 3037 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6268
XbaI T'CTAG_A
Cuts at: 2173 2371 3298 3604 2173
Size: 198 927 306 4837
Fragments arranged by size:
4837 927 306 198
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6268
XmaI C'CCGG_G
Cuts at: 1870 3593 1870
Size: 1723 4545
XmnI GAAnn'nnTTC
Cuts at: 4397 4397
Size: 6268
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4076 276
Size: 53 83 186 936 2542 2468
Fragments arranged by size:
2542 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI