Restriction map for
pFN21AB5886
(Circular) (Six-base) MAPSORT of: pFN21AB5886.seq Check: 9266 from: 1 to: 6196
pFN21AB5886 6196 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2505 3076 2505
Size: 571 5625
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4006 278
Size: 53 83 186 936 2470 2468
Fragments arranged by size:
2470 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2379 3539 1937
Size: 442 1160 4594
Fragments arranged by size:
4594 1160 442
Acc65I G'GTAC_C
Cuts at: 3517 3517
Size: 6196
AclI AA'CG_TT
Cuts at: 1563 4323 4696 1563
Size: 2760 373 3063
Fragments arranged by size:
3063 2760 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2478 2834 4259 5643 5911 1632
Size: 846 356 1425 1384 268 1917
Fragments arranged by size:
1917 1425 1384 846 356 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5999
AflIII A'CryG_T
Cuts at: 1203 5095 6072 1203
Size: 3892 977 1327
Fragments arranged by size:
3892 1327 977
AgeI A'CCGG_T
Cuts at: 5027 5027
Size: 6196
AhdI GACnn_n'nnGTC
Cuts at: 4925 4925
Size: 6196
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6196
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6196
AlwNI CAG_nnn'CTG
Cuts at: 2836 2920 3939 5511 2836
Size: 84 1019 1572 3521
Fragments arranged by size:
3521 1572 1019 84
ApaLI G'TGCA_C
Cuts at: 4253 5409 4253
Size: 1156 5040
ApoI r'AATT_y
Cuts at: 1493 2447 2738 3505 3731 3883 5992 1493
Size: 954 291 767 226 152 2109 1697
Fragments arranged by size:
2109 1697 954 767 291 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4750 160
Size: 872 3718 1606
Fragments arranged by size:
3718 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
AvaI C'yCGr_G
Cuts at: 1870 1943 2054 2355 2625 3521 1870
Size: 73 111 301 270 896 4545
Fragments arranged by size:
4545 896 301 270 111 73
AvrII C'CTAG_G
Cuts at: 2779 2779
Size: 6196
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3240 3273 3240
Size: 33 6163
BamHI G'GATC_C
Cuts at: 2620 3526 3893 2620
Size: 906 367 4923
Fragments arranged by size:
4923 906 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3517 4972 618
Size: 325 217 627 1730 1455 1842
Fragments arranged by size:
1842 1730 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2122 2181 2376 2472 2540 3048 3075 3136
Size: 731 59 195 96 68 508 27 61
Cuts at: 3136 3321 3515 1391
Size: 185 194 4072
Fragments arranged by size:
4072 731 508 195 194 185 96 68
61 59 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2822 2875 928
Size: 971 923 53 4249
Fragments arranged by size:
4249 971 923 53
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3074 3108 4387 4421 1903
Size: 34 1137 34 1279 34 3678
Fragments arranged by size:
3678 1279 1137 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4088 5298 4088
Size: 1210 4986
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6196
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2488 2897 3443
Size: 107 122 71 631 605 816 409 546
Cuts at: 3443 4807 136
Size: 1364 1525
Fragments arranged by size:
1525 1364 816 631 605 546 409 122
107 71
BglII A'GATC_T
Cuts at: 6191 6191
Size: 6196
BlpI GC'TnA_GC
Cuts at: 2234 2763 3613 2234
Size: 529 850 4817
Fragments arranged by size:
4817 850 529
Bme1580I G_kGCm'C
Cuts at: 1790 2073 4257 5413 1790
Size: 283 2184 1156 2573
Fragments arranged by size:
2573 2184 1156 283
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6196
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2245 2427 3046
Size: 416 53 83 399 24 828 182 619
Cuts at: 3046 3927 4885 442
Size: 881 958 1753
Fragments arranged by size:
1753 958 881 828 619 416 399 182
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2621 2792 2942 3416 3437 4856 1239
Size: 1382 171 150 474 21 1419 2579
Fragments arranged by size:
2579 1419 1382 474 171 150 21
Bpu10I CC'TnA_GC
Cuts at: 3164 3164
Size: 6196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2279 2498 2627 3263 4321 5186 5484 2279
Size: 219 129 636 1058 865 298 2991
Fragments arranged by size:
2991 1058 865 636 298 219 129
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2687 4859 882
Size: 617 1188 2172 2219
Fragments arranged by size:
2219 2172 1188 617
BsaAI yAC'GTr
Cuts at: 493 1204 2950 3987 493
Size: 711 1746 1037 2702
Fragments arranged by size:
2702 1746 1037 711
BsaBI GATnn'nnATC
Cuts at: 6190 6190
Size: 6196
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4003 4385 5020 275
Size: 53 83 186 936 2470 382 635 1451
Fragments arranged by size:
2470 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2418 2724 4629 5027 5301 5448 5859 2418
Size: 306 1905 398 274 147 411 2755
Fragments arranged by size:
2755 1905 411 398 306 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2081 2111 2621 2651 2990
Size: 30 165 30 678 30 510 30 339
Cuts at: 2990 3020 3212 3242 1178
Size: 30 192 30 4132
Fragments arranged by size:
4132 678 510 339 192 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2038 2134 3413 1186
Size: 852 96 1279 3969
Fragments arranged by size:
3969 1279 852 96
BseYI C'CCAG_C
Cuts at: 2892 5399 5895 2892
Size: 2507 496 3193
Fragments arranged by size:
3193 2507 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2207 2639 2843 2933 2971 1163
Size: 573 471 432 204 90 38 4388
Fragments arranged by size:
4388 573 471 432 204 90 38
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3646 4407 5435 1540
Size: 275 186 1645 761 1028 2301
Fragments arranged by size:
2301 1645 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2181 2354 2748 2826 2949 3240 3279 3321 4257
Size: 173 394 78 123 291 39 42 936
Cuts at: 4257 4342 5413 2181
Size: 85 1071 2964
Fragments arranged by size:
2964 1071 936 394 291 173 123 85
78 42 39
BsmI GAATG_Cn'
Cuts at: 3707 3800 3707
Size: 93 6103
BsmBI CGTCTCn'nnnn_
Cuts at: 2003 2003
Size: 6196
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2122 2181 2354 2376 2472 2540
Size: 399 283 49 59 173 22 96 68
Cuts at: 2540 2748 2826 2949 3048 3075 3136 3240 3279
Size: 208 78 123 99 27 61 104 39
Cuts at: 3279 3321 3515 4257 4342 5413 1391
Size: 42 194 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 283 208 194 173
123 104 99 96 85 78 68 61
59 49 42 39 27 22
BspEI T'CCGG_A
Cuts at: 2418 5859 2418
Size: 3441 2755
BspHI T'CATG_A
Cuts at: 4085 5815 4085
Size: 1730 4466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2505 2993 3076 3551 844
Size: 1661 488 83 475 3489
Fragments arranged by size:
3489 1661 488 475 83
BsrBI CCG'CTC
Cuts at: 1394 1823 2133 3130 4083 1394
Size: 429 310 997 953 3507
Fragments arranged by size:
3507 997 953 429 310
BsrDI GCAATG_nn'
Cuts at: 65 1248 4691 4865 65
Size: 1183 3443 174 1396
Fragments arranged by size:
3443 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2209 3414 4840 5027 1700
Size: 110 399 1205 1426 187 2869
Fragments arranged by size:
2869 1426 1205 399 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6196
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6196
BssSI C'ACGA_G
Cuts at: 2225 4256 5268 2225
Size: 2031 1012 3153
Fragments arranged by size:
3153 2031 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2230 2706 794
Size: 1436 476 4284
Fragments arranged by size:
4284 1436 476
BstBI TT'CG_AA
Cuts at: 5000 5000
Size: 6196
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6196
BstYI r'GATC_y
Cuts at: 1972 2620 2775 3063 3526 3893 4278 4295 5747
Size: 648 155 288 463 367 385 17 1452
Cuts at: 5747 5833 5845 6191 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 648 463 385 367 346 288
155 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2556 513
Size: 552 939 552 4153
Fragments arranged by size:
4153 939 552 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5457
Fragments arranged by size:
5457 683 56
BtsI GCAGTG_nn'
Cuts at: 3712 4506 4526 3712
Size: 794 20 5382
Fragments arranged by size:
5382 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6196
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6161
DraI TTT'AAA
Cuts at: 3500 3500
Size: 6196
DraIII CAC_nnn'GTG
Cuts at: 3193 3193
Size: 6196
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5203 809
Size: 507 3887 1802
Fragments arranged by size:
3887 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2286 2489 2559
Size: 54 1425 50 275 18 456 203 70
Cuts at: 2559 2975 3474 3643 4532 6161 8
Size: 416 499 169 889 1629 43
Fragments arranged by size:
1629 1425 889 499 456 416 275 203
169 70 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3643 1537
Size: 275 1831 4090
Fragments arranged by size:
4090 1831 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2454 3018 4126 1353
Size: 1101 564 1108 3423
Fragments arranged by size:
3423 1108 1101 564
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4783 5157 5303 387
Size: 1064 138 3194 374 146 1280
Fragments arranged by size:
3194 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2179 3319 2179
Size: 1140 5056
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2478 2621 2792 2834 2942 3416 3437
Size: 393 846 143 171 42 108 474 21
Cuts at: 3437 4259 4856 5643 5911 1239
Size: 822 597 787 268 1524
Fragments arranged by size:
1524 846 822 787 597 474 393 268
171 143 108 42 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2924 3173 2924
Size: 249 5947
EcoO109I rG'GnC_Cy
Cuts at: 3213 3426 3445 3213
Size: 213 19 5964
Fragments arranged by size:
5964 213 19
EcoRI G'AATT_C
Cuts at: 2738 3505 3883 2738
Size: 767 378 5051
Fragments arranged by size:
5051 767 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3047 3047
Size: 6196
FspI TGC'GCA
Cuts at: 4702 4702
Size: 6196
HaeII r_GCGC'y
Cuts at: 1678 5343 1678
Size: 3665 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2081 2113 4837 4869 4911
Size: 32 135 32 509 32 2724 32 42
Cuts at: 4911 4943 1373
Size: 32 2626
Fragments arranged by size:
2724 2626 509 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3540 3786 669
Size: 988 281 1602 246 3079
Fragments arranged by size:
3079 1602 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6196
HpaI GTT'AAC
Cuts at: 3786 3786
Size: 6196
KpnI G_GTAC'C
Cuts at: 3521 3521
Size: 6196
MfeI C'AATT_G
Cuts at: 3795 3795
Size: 6196
MluI A'CGCG_T
Cuts at: 6072 6072
Size: 6196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5310 5494 1305
Size: 4005 184 2007
Fragments arranged by size:
4005 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2491 2561 2977 10
Size: 54 1425 343 659 70 416 3229
Fragments arranged by size:
3229 1425 659 416 343 70 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2396 2751 3293 4154 4513 4672 518
Size: 648 1230 355 542 861 359 159 2042
Fragments arranged by size:
2042 1230 861 648 542 359 355 159
MspA1I CmG'CkG
Cuts at: 1606 2188 2503 2896 3457 3612 4289 5437 5682
Size: 582 315 393 561 155 677 1148 245
Cuts at: 5682 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 582 561 393 315 245
155
NaeI GCC'GGC
Cuts at: 2211 3416 2211
Size: 1205 4991
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4705
Fragments arranged by size:
4705 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6196
NgoMIV G'CCGG_C
Cuts at: 2209 3414 2209
Size: 1205 4991
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6196
NotI GC'GGCC_GC
Cuts at: 3643 3643
Size: 6196
NspI r_CATG'y
Cuts at: 1132 1229 3554 5099 1132
Size: 97 2325 1545 2229
Fragments arranged by size:
2325 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3430 1348
Size: 2082 4114
PciI A'CATG_T
Cuts at: 5095 5095
Size: 6196
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6196
PfoI T'CCnGG_A
Cuts at: 2598 2919 3066 2598
Size: 321 147 5728
Fragments arranged by size:
5728 321 147
PmeI GTTT'AAAC
Cuts at: 3500 3500
Size: 6196
PmlI CAC'GTG
Cuts at: 2950 2950
Size: 6196
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4265 4297 5778 5810 4265
Size: 32 1481 32 4651
Fragments arranged by size:
4651 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3213 3426 3213
Size: 213 5983
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6196
PsiI TTA'TAA
Cuts at: 3766 3766
Size: 6196
PstI C_TGCA'G
Cuts at: 830 2502 3548 830
Size: 1672 1046 3478
Fragments arranged by size:
3478 1672 1046
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6196
PvuII CAG'CTG
Cuts at: 2503 2896 2503
Size: 393 5803
SacI G_AGCT'C
Cuts at: 2181 3321 2181
Size: 1140 5056
SalI G'TCGA_C
Cuts at: 1936 3538 1936
Size: 1602 4594
SanDI GG'GwC_CC
Cuts at: 3213 3426 3213
Size: 213 5983
SbfI CC_TGCA'GG
Cuts at: 3548 3548
Size: 6196
ScaI AGT'ACT
Cuts at: 4444 6156 4444
Size: 1712 4484
SfcI C'TryA_G
Cuts at: 826 1045 2498 3419 3544 4679 5360 5551 826
Size: 219 1453 921 125 1135 681 191 1471
Fragments arranged by size:
1471 1453 1135 921 681 219 191 125
SfiI GGCCn_nnn'nGGCC
Cuts at: 3443 3443
Size: 6196
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6196
SmaI CCC'GGG
Cuts at: 1872 2056 2357 2627 3523 1872
Size: 184 301 270 896 4545
Fragments arranged by size:
4545 896 301 270 184
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2294 2477 2642 3278 4300 5201
Size: 197 926 351 183 165 636 1022 901
Cuts at: 5201 5463 820
Size: 262 1553
Fragments arranged by size:
1553 1022 926 901 636 351 262 197
183 165
SnaBI TAC'GTA
Cuts at: 493 3987 493
Size: 3494 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6196
SphI G_CATG'C
Cuts at: 1132 3554 1132
Size: 2422 3774
SspI AAT'ATT
Cuts at: 5 52 4120 5
Size: 47 4068 2081
Fragments arranged by size:
4068 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6196
StyI C'CwwG_G
Cuts at: 513 1065 2004 2779 3231 3438 3635 513
Size: 552 939 775 452 207 197 3074
Fragments arranged by size:
3074 939 775 552 452 207 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3005 4223 4240 4393 4578 5023 325
Size: 1255 1425 1218 17 153 185 445 1498
Fragments arranged by size:
1498 1425 1255 1218 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4442 6154 96
Size: 274 80 33 51 1443 2465 1712 138
Fragments arranged by size:
2465 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6156 6188 6156
Size: 32 6164
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6196
XbaI T'CTAG_A
Cuts at: 3532 3532
Size: 6196
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2569 2569
Size: 6196
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6196
XmaI C'CCGG_G
Cuts at: 1870 2054 2355 2625 3521 1870
Size: 184 301 270 896 4545
Fragments arranged by size:
4545 896 301 270 184
XmnI GAAnn'nnTTC
Cuts at: 2414 3027 4325 2414
Size: 613 1298 4285
Fragments arranged by size:
4285 1298 613
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4004 276
Size: 53 83 186 936 2470 2468
Fragments arranged by size:
2470 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BbeI BbvCI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII
SacII SapI SexAI SfoI SrfI SwaI