Restriction map for
pFN21AB5882
(Circular) (Six-base) MAPSORT of: pFN21AB5882.seq Check: 9175 from: 1 to: 6193
pFN21AB5882 6193 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4003 278
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3536 1937
Size: 1599 4594
Acc65I G'GTAC_C
Cuts at: 3514 3514
Size: 6193
AclI AA'CG_TT
Cuts at: 1563 4320 4693 1563
Size: 2757 373 3063
Fragments arranged by size:
3063 2757 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2564 2747 2785 4256 5640 5908 1632
Size: 932 183 38 1471 1384 268 1917
Fragments arranged by size:
1917 1471 1384 932 268 183 38
AfeI AGC'GCT
Cuts at: 2561 3332 2561
Size: 771 5422
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5996
AflIII A'CryG_T
Cuts at: 1203 5092 6069 1203
Size: 3889 977 1327
Fragments arranged by size:
3889 1327 977
AgeI A'CCGG_T
Cuts at: 5024 5024
Size: 6193
AhdI GACnn_n'nnGTC
Cuts at: 2382 4922 2382
Size: 2540 3653
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6193
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6193
AlwNI CAG_nnn'CTG
Cuts at: 2677 3936 5508 2677
Size: 1259 1572 3362
Fragments arranged by size:
3362 1572 1259
ApaI G_GGCC'C
Cuts at: 2270 2820 3426 2270
Size: 550 606 5037
Fragments arranged by size:
5037 606 550
ApaLI G'TGCA_C
Cuts at: 4250 5406 4250
Size: 1156 5037
ApoI r'AATT_y
Cuts at: 1493 2948 3502 3728 3880 5989 1493
Size: 1455 554 226 152 2109 1697
Fragments arranged by size:
2109 1697 1455 554 226 152
AscI GG'CGCG_CC
Cuts at: 2412 2412
Size: 6193
AseI AT'TA_AT
Cuts at: 160 1032 4747 160
Size: 872 3715 1606
Fragments arranged by size:
3715 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
AvaI C'yCGr_G
Cuts at: 1870 1943 2023 2208 2272 2341 2354 2418 3334
Size: 73 80 185 64 69 13 64 916
Cuts at: 3334 3353 3518 1870
Size: 19 165 4545
Fragments arranged by size:
4545 916 185 165 80 73 69 64
64 19 13
BamHI G'GATC_C
Cuts at: 3523 3890 3523
Size: 367 5826
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2231 2250 2626 3292 3514
Size: 325 217 627 444 19 376 666 222
Cuts at: 3514 4969 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 666 627 444 376 325 222
217 19
BanII G_rGCy'C
Cuts at: 1391 2270 2312 2820 3195 3360 3426 3512 1391
Size: 879 42 508 375 165 66 86 4072
Fragments arranged by size:
4072 879 508 375 165 86 66 42
BbeI G_GCGC'C
Cuts at: 2235 2254 2235
Size: 19 6174
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5222
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3145 3179 3388 3422 4384 4418 1903
Size: 34 1208 34 209 34 962 34 3678
Fragments arranged by size:
3678 1208 962 209 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4085 5295 4085
Size: 1210 4983
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6193
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2147 2447 2652
Size: 107 122 71 631 605 475 300 205
Cuts at: 2652 4804 136
Size: 2152 1525
Fragments arranged by size:
2152 1525 631 605 475 300 205 122
107 71
BglII A'GATC_T
Cuts at: 6188 6188
Size: 6193
BlpI GC'TnA_GC
Cuts at: 2133 3182 3610 2133
Size: 1049 428 4716
Fragments arranged by size:
4716 1049 428
Bme1580I G_kGCm'C
Cuts at: 1790 2270 2820 3297 3426 4254 5410 1790
Size: 480 550 477 129 828 1156 2573
Fragments arranged by size:
2573 1156 828 550 480 477 129
BmgBI CAC'GTC
Cuts at: 1318 2323 1318
Size: 1005 5188
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3022 3924 4882
Size: 416 53 83 399 24 1605 902 958
Cuts at: 4882 442
Size: 1753
Fragments arranged by size:
1753 1605 958 902 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2474 2528 2600 3143 3488 4853 1239
Size: 1235 54 72 543 345 1365 2579
Fragments arranged by size:
2579 1365 1235 543 345 72 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2537 4318 5183 5481 2537
Size: 1781 865 298 3249
Fragments arranged by size:
3249 1781 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4856 882
Size: 617 3357 2219
Fragments arranged by size:
3357 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2532 3984 493
Size: 711 1328 1452 2702
Fragments arranged by size:
2702 1452 1328 711
BsaBI GATnn'nnATC
Cuts at: 6187 6187
Size: 6193
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2232 2251 2403 2424
Size: 53 83 186 936 699 19 152 21
Cuts at: 2424 2958 2976 3363 4000 4382 5017 275
Size: 534 18 387 637 382 635 1451
Fragments arranged by size:
1451 936 699 637 635 534 387 382
186 152 83 53 21 19 18
BsaWI w'CCGG_w
Cuts at: 2971 3021 4626 5024 5298 5445 5856 2971
Size: 50 1605 398 274 147 411 3308
Fragments arranged by size:
3308 1605 411 398 274 147 50
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5968
Fragments arranged by size:
5968 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2844 3467 1186
Size: 1658 623 3912
Fragments arranged by size:
3912 1658 623
BseYI C'CCAG_C
Cuts at: 2303 3417 5396 5892 2303
Size: 1114 1979 496 2604
Fragments arranged by size:
2604 1979 1114 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3019 1163
Size: 573 1283 4337
Fragments arranged by size:
4337 1283 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2068 2173 2686 3271 3643 4404
Size: 275 186 67 105 513 585 372 761
Cuts at: 4404 5432 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 585 513 372 275 186
105 67
BsiHKAI G_wGCw'C
Cuts at: 2312 3021 3228 4254 4339 5410 2312
Size: 709 207 1026 85 1071 3095
Fragments arranged by size:
3095 1071 1026 709 207 85
BsmI GAATG_Cn'
Cuts at: 3704 3797 3704
Size: 93 6100
BsmBI CGTCTCn'nnnn_
Cuts at: 2363 2363
Size: 6193
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2270 2312 2820 3021 3195 3228 3297
Size: 399 480 42 508 201 174 33 69
Cuts at: 3297 3360 3426 3512 4254 4339 5410 1391
Size: 63 66 86 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 508 480 399 201 174
86 85 69 66 63 42 33
BspEI T'CCGG_A
Cuts at: 2971 5856 2971
Size: 2885 3308
BspHI T'CATG_A
Cuts at: 4082 5812 4082
Size: 1730 4463
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2387 2715 3548 844
Size: 1543 328 833 3489
Fragments arranged by size:
3489 1543 833 328
BsrBI CCG'CTC
Cuts at: 1394 1823 2175 2398 4080 1394
Size: 429 352 223 1682 3507
Fragments arranged by size:
3507 1682 429 352 223
BsrDI GCAATG_nn'
Cuts at: 65 1248 4688 4862 65
Size: 1183 3440 174 1396
Fragments arranged by size:
3440 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2137 2253 2297 2408 2458 2805 4837
Size: 110 327 116 44 111 50 347 2032
Cuts at: 4837 5024 1700
Size: 187 2869
Fragments arranged by size:
2869 2032 347 327 187 116 111 110
50 44
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6193
BssHII G'CGCG_C
Cuts at: 1925 2257 2412 2486 2632 1925
Size: 332 155 74 146 5486
Fragments arranged by size:
5486 332 155 146 74
BssSI C'ACGA_G
Cuts at: 4253 5265 4253
Size: 1012 5181
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2677 794
Size: 1883 4310
BstBI TT'CG_AA
Cuts at: 4997 4997
Size: 6193
BstEII G'GTnAC_C
Cuts at: 1184 2374 1184
Size: 1190 5003
BstYI r'GATC_y
Cuts at: 1972 2878 3471 3523 3890 4275 4292 5744 5830
Size: 906 593 52 367 385 17 1452 86
Cuts at: 5830 5842 6188 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 906 593 385 367 346 86
52 17 12
Bsu36I CC'TnA_GG
Cuts at: 3087 3087
Size: 6193
BtgI C'CryG_G
Cuts at: 513 1065 2144 2198 2596 2718 3217 513
Size: 552 1079 54 398 122 499 3489
Fragments arranged by size:
3489 1079 552 499 398 122 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5454
Fragments arranged by size:
5454 683 56
BtsI GCAGTG_nn'
Cuts at: 2223 2934 3709 4503 4523 2223
Size: 711 775 794 20 3893
Fragments arranged by size:
3893 794 775 711 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6158
DraI TTT'AAA
Cuts at: 3497 3497
Size: 6193
DraIII CAC_nnn'GTG
Cuts at: 2719 2719
Size: 6193
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3062 5200 809
Size: 507 1746 2138 1802
Fragments arranged by size:
2138 1802 1746 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2170 2439 2653
Size: 54 1425 50 275 18 340 269 214
Cuts at: 2653 2683 3268 3640 4529 6158 8
Size: 30 585 372 889 1629 43
Fragments arranged by size:
1629 1425 889 585 372 340 275 269
214 54 50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2170 2683 3268 3640 1537
Size: 275 358 513 585 372 4090
Fragments arranged by size:
4090 585 513 372 358 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4123 1353
Size: 2770 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2174 2175 2342 2488 2633 2817
Size: 1064 138 585 1 167 146 145 184
Cuts at: 2817 4780 5154 5300 387
Size: 1963 374 146 1280
Fragments arranged by size:
1963 1280 1064 585 374 184 167 146
146 145 138 1
EcoICRI GAG'CTC
Cuts at: 2310 2310
Size: 6193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2474 2528 2564 2600 2747 2785 3143
Size: 393 842 54 36 36 147 38 358
Cuts at: 3143 3488 4256 4853 5640 5908 1239
Size: 345 768 597 787 268 1524
Fragments arranged by size:
1524 842 787 768 597 393 358 345
268 147 54 38 36 36
EcoNI CCTnn'n_nnAGG
Cuts at: 3086 3086
Size: 6193
EcoO109I rG'GnC_Cy
Cuts at: 2266 2267 3422 3423 2266
Size: 1 1155 1 5036
Fragments arranged by size:
5036 1155 1 1
EcoRI G'AATT_C
Cuts at: 2948 3502 3880 2948
Size: 554 378 5261
Fragments arranged by size:
5261 554 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3184 3184
Size: 6193
FspI TGC'GCA
Cuts at: 3287 4699 3287
Size: 1412 4781
HaeII r_GCGC'y
Cuts at: 1678 2235 2254 2304 2563 3334 5340 1678
Size: 557 19 50 259 771 2006 2531
Fragments arranged by size:
2531 2006 771 557 259 50 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2368 2400 4834 4866 4908
Size: 32 135 32 796 32 2434 32 42
Cuts at: 4908 4940 1373
Size: 32 2626
Fragments arranged by size:
2626 2434 796 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3241 3537 3783 669
Size: 988 281 1303 296 246 3079
Fragments arranged by size:
3079 1303 988 296 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6193
HpaI GTT'AAC
Cuts at: 3783 3783
Size: 6193
KasI G'GCGC_C
Cuts at: 2231 2250 2231
Size: 19 6174
KpnI G_GTAC'C
Cuts at: 3518 3518
Size: 6193
MfeI C'AATT_G
Cuts at: 3792 3792
Size: 6193
MluI A'CGCG_T
Cuts at: 6069 6069
Size: 6193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2265 5307 5491 1305
Size: 960 3042 184 2007
Fragments arranged by size:
3042 2007 960 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4371
Fragments arranged by size:
4371 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3314 4151 4510 4669 518
Size: 648 2148 837 359 159 2042
Fragments arranged by size:
2148 2042 837 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2085 2146 2200 2598 2674 3096 3219 3609
Size: 479 61 54 398 76 422 123 390
Cuts at: 3609 4286 5434 5679 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 479 422 398 390 245
123 76 61 54
NaeI GCC'GGC
Cuts at: 2139 2255 2299 2410 2460 2807 2139
Size: 116 44 111 50 347 5525
Fragments arranged by size:
5525 347 116 111 50 44
NarI GG'CG_CC
Cuts at: 2232 2251 2232
Size: 19 6174
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5641
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6193
NgoMIV G'CCGG_C
Cuts at: 2137 2253 2297 2408 2458 2805 2137
Size: 116 44 111 50 347 5525
Fragments arranged by size:
5525 347 116 111 50 44
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6193
NotI GC'GGCC_GC
Cuts at: 2170 3268 3640 2170
Size: 1098 372 4723
Fragments arranged by size:
4723 1098 372
NruI TCG'CGA
Cuts at: 2481 2859 2481
Size: 378 5815
NspI r_CATG'y
Cuts at: 1132 1229 2968 3551 5096 1132
Size: 97 1739 583 1545 2229
Fragments arranged by size:
2229 1739 1545 583 97
PasI CC'CwG_GG
Cuts at: 1348 2236 1348
Size: 888 5305
PciI A'CATG_T
Cuts at: 5092 5092
Size: 6193
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3417 1108
Size: 2309 3884
PmeI GTTT'AAAC
Cuts at: 3497 3497
Size: 6193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4262 4294 5775 5807 4262
Size: 32 1481 32 4648
Fragments arranged by size:
4648 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6193
PsiI TTA'TAA
Cuts at: 3763 3763
Size: 6193
PspOMI G'GGCC_C
Cuts at: 2266 2816 3422 2266
Size: 550 606 5037
Fragments arranged by size:
5037 606 550
PstI C_TGCA'G
Cuts at: 830 2712 3345 3545 830
Size: 1882 633 200 3478
Fragments arranged by size:
3478 1882 633 200
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6193
PvuII CAG'CTG
Cuts at: 2674 2674
Size: 6193
SacI G_AGCT'C
Cuts at: 2312 2312
Size: 6193
SacII CC_GC'GG
Cuts at: 2147 2201 2599 3220 2147
Size: 54 398 621 5120
Fragments arranged by size:
5120 621 398 54
SalI G'TCGA_C
Cuts at: 1936 3535 1936
Size: 1599 4594
SbfI CC_TGCA'GG
Cuts at: 3545 3545
Size: 6193
ScaI AGT'ACT
Cuts at: 4441 6153 4441
Size: 1712 4481
SfcI C'TryA_G
Cuts at: 826 1045 2708 3341 3541 4676 5357 5548 826
Size: 219 1663 633 200 1135 681 191 1471
Fragments arranged by size:
1663 1471 1135 681 633 219 200 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2447 2447
Size: 6193
SfoI GGC'GCC
Cuts at: 2233 2252 2233
Size: 19 6174
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
SgrAI Cr'CCGG_yG
Cuts at: 1700 2253 2408 1700
Size: 553 155 5485
Fragments arranged by size:
5485 553 155
SmaI CCC'GGG
Cuts at: 1872 2210 2343 2420 3520 1872
Size: 338 133 77 1100 4545
Fragments arranged by size:
4545 1100 338 133 77
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2552 4297 5198 5460 820
Size: 197 926 609 1745 901 262 1553
Fragments arranged by size:
1745 1553 926 901 609 262 197
SnaBI TAC'GTA
Cuts at: 493 3984 493
Size: 3491 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6193
SphI G_CATG'C
Cuts at: 1132 3551 1132
Size: 2419 3774
SrfI GCCC'GGGC
Cuts at: 2343 2343
Size: 6193
SspI AAT'ATT
Cuts at: 5 52 4117 5
Size: 47 4065 2081
Fragments arranged by size:
4065 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6193
StyI C'CwwG_G
Cuts at: 513 1065 3411 3632 513
Size: 552 2346 221 3074
Fragments arranged by size:
3074 2346 552 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4220 4237 4390 4575 5020 325
Size: 1255 2640 17 153 185 445 1498
Fragments arranged by size:
2640 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2865 4439 6151
Size: 274 80 33 51 1443 888 1574 1712
Cuts at: 6151 96
Size: 138
Fragments arranged by size:
1712 1574 1443 888 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6153 6185 6153
Size: 32 6161
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6193
XbaI T'CTAG_A
Cuts at: 3529 3529
Size: 6193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2434 2434
Size: 6193
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6193
XmaI C'CCGG_G
Cuts at: 1870 2208 2341 2418 3518 1870
Size: 338 133 77 1100 4545
Fragments arranged by size:
4545 1100 338 133 77
XmnI GAAnn'nnTTC
Cuts at: 4322 4322
Size: 6193
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4001 276
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BstXI BstZ17I EcoRV FseI FspAI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SapI
SexAI SwaI