Restriction map for
pFN21AB5878
(Circular) (Six-base) MAPSORT of: pFN21AB5878.seq Check: 5593 from: 1 to: 6190
pFN21AB5878 6190 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4000 278
Size: 53 83 186 936 2464 2468
Fragments arranged by size:
2468 2464 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3533 1937
Size: 1596 4594
Acc65I G'GTAC_C
Cuts at: 3511 3511
Size: 6190
AclI AA'CG_TT
Cuts at: 1563 4317 4690 1563
Size: 2754 373 3063
Fragments arranged by size:
3063 2754 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2882 4253 5637 5905 1632
Size: 1250 1371 1384 268 1917
Fragments arranged by size:
1917 1384 1371 1250 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5993
AflIII A'CryG_T
Cuts at: 1203 2083 5089 6066 1203
Size: 880 3006 977 1327
Fragments arranged by size:
3006 1327 977 880
AgeI A'CCGG_T
Cuts at: 5021 5021
Size: 6190
AhdI GACnn_n'nnGTC
Cuts at: 4919 4919
Size: 6190
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6190
AlwNI CAG_nnn'CTG
Cuts at: 3933 5505 3933
Size: 1572 4618
ApaLI G'TGCA_C
Cuts at: 4247 5403 4247
Size: 1156 5034
ApoI r'AATT_y
Cuts at: 1493 2023 3149 3474 3499 3725 3877 5986 1493
Size: 530 1126 325 25 226 152 2109 1697
Fragments arranged by size:
2109 1697 1126 530 325 226 152 25
AseI AT'TA_AT
Cuts at: 160 1032 3176 3324 4744 160
Size: 872 2144 148 1420 1606
Fragments arranged by size:
2144 1606 1420 872 148
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
AvaI C'yCGr_G
Cuts at: 1870 1943 3515 1870
Size: 73 1572 4545
Fragments arranged by size:
4545 1572 73
BamHI G'GATC_C
Cuts at: 3520 3887 3520
Size: 367 5823
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3511 4966 618
Size: 325 217 627 1724 1455 1842
Fragments arranged by size:
1842 1724 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3509 1391
Size: 2118 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2541 928
Size: 971 642 4577
Fragments arranged by size:
4577 971 642
BbvCI CC'TCA_GC
Cuts at: 3156 3156
Size: 6190
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4381 4415 1903
Size: 34 2444 34 3678
Fragments arranged by size:
3678 2444 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4082 5292 4082
Size: 1210 4980
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6190
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4801 136
Size: 107 122 71 631 605 3129 1525
Fragments arranged by size:
3129 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2670 6185 2670
Size: 3515 2675
BlpI GC'TnA_GC
Cuts at: 3607 3607
Size: 6190
Bme1580I G_kGCm'C
Cuts at: 1790 4251 5407 1790
Size: 2461 1156 2573
Fragments arranged by size:
2573 2461 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6190
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3921 4879 442
Size: 416 53 83 399 24 2504 958 1753
Fragments arranged by size:
2504 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3146 1056
Size: 2090 4100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2243 2788 4850 1239
Size: 1004 545 2062 2579
Fragments arranged by size:
2579 2062 1004 545
Bpu10I CC'TnA_GC
Cuts at: 3156 3156
Size: 6190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4315 5180 5478 4315
Size: 865 298 5027
Fragments arranged by size:
5027 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4853 882
Size: 617 3354 2219
Fragments arranged by size:
3354 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3981 493
Size: 711 2777 2702
Fragments arranged by size:
2777 2702 711
BsaBI GATnn'nnATC
Cuts at: 3426 6184 3426
Size: 2758 3432
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3997 4379 5014 275
Size: 53 83 186 936 2464 382 635 1451
Fragments arranged by size:
2464 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4623 5021 5295 5442 5853 4623
Size: 398 274 147 411 4960
Fragments arranged by size:
4960 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5965
Fragments arranged by size:
5965 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2801 2894 3134 1186
Size: 1615 93 240 4242
Fragments arranged by size:
4242 1615 240 93
BseYI C'CCAG_C
Cuts at: 5393 5889 5393
Size: 496 5694
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 1163
Size: 573 417 5200
Fragments arranged by size:
5200 573 417
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3640 4401 5429 1540
Size: 275 186 1639 761 1028 2301
Fragments arranged by size:
2301 1639 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4251 4336 5407 4251
Size: 85 1071 5034
Fragments arranged by size:
5034 1071 85
BsmI GAATG_Cn'
Cuts at: 2530 2725 2908 3187 3701 3794 2530
Size: 195 183 279 514 93 4926
Fragments arranged by size:
4926 514 279 195 183 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3509 4251 4336 5407 1391
Size: 399 1719 742 85 1071 2174
Fragments arranged by size:
2174 1719 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5853 5853
Size: 6190
BspHI T'CATG_A
Cuts at: 2646 4079 5809 2646
Size: 1433 1730 3027
Fragments arranged by size:
3027 1730 1433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3545 844
Size: 2701 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4077 1394
Size: 429 2254 3507
Fragments arranged by size:
3507 2254 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2730 4685 4859 65
Size: 1183 1482 1955 174 1396
Fragments arranged by size:
1955 1482 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4834 5021 1700
Size: 110 3024 187 2869
Fragments arranged by size:
3024 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6190
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6190
BssSI C'ACGA_G
Cuts at: 4250 5262 4250
Size: 1012 5178
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6190
BstBI TT'CG_AA
Cuts at: 4994 4994
Size: 6190
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6190
BstYI r'GATC_y
Cuts at: 1972 2670 3520 3887 4272 4289 5741 5827 5839
Size: 698 850 367 385 17 1452 86 12
Cuts at: 5839 6185 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 850 698 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4699
Fragments arranged by size:
4699 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5451
Fragments arranged by size:
5451 683 56
BtsI GCAGTG_nn'
Cuts at: 3706 4500 4520 3706
Size: 794 20 5376
Fragments arranged by size:
5376 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6190
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3259 3294 529
Size: 35 2695 35 3425
Fragments arranged by size:
3425 2695 35 35
DraI TTT'AAA
Cuts at: 3409 3494 3409
Size: 85 6105
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5197 809
Size: 507 3881 1802
Fragments arranged by size:
3881 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3637 4526 6155
Size: 54 1425 50 275 18 1807 889 1629
Cuts at: 6155 8
Size: 43
Fragments arranged by size:
1807 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3637 1537
Size: 275 1825 4090
Fragments arranged by size:
4090 1825 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2208 2514 2736 3111 3237 3282 3435 3477
Size: 855 306 222 375 126 45 153 42
Cuts at: 3477 4120 1353
Size: 643 3423
Fragments arranged by size:
3423 855 643 375 306 222 153 126
45 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4777 5151 5297 387
Size: 1064 138 3188 374 146 1280
Fragments arranged by size:
3188 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2243 2788 2882 4253 4850 5637 5905
Size: 393 611 545 94 1371 597 787 268
Cuts at: 5905 1239
Size: 1524
Fragments arranged by size:
1524 1371 787 611 597 545 393 268
94
EcoRI G'AATT_C
Cuts at: 3499 3877 3499
Size: 378 5812
FspI TGC'GCA
Cuts at: 4696 4696
Size: 6190
HaeII r_GCGC'y
Cuts at: 1678 5337 1678
Size: 3659 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4831 4863 4905 4937 1373
Size: 32 135 32 3259 32 42 32 2626
Fragments arranged by size:
3259 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2107 2910 3534 3780 669
Size: 988 281 169 803 624 246 3079
Fragments arranged by size:
3079 988 803 624 281 246 169
HindIII A'AGCT_T
Cuts at: 748 2616 748
Size: 1868 4322
HpaI GTT'AAC
Cuts at: 3780 3780
Size: 6190
KpnI G_GTAC'C
Cuts at: 3515 3515
Size: 6190
MfeI C'AATT_G
Cuts at: 3789 3789
Size: 6190
MluI A'CGCG_T
Cuts at: 6066 6066
Size: 6190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2184 2670 5304 5488 1305
Size: 879 486 2634 184 2007
Fragments arranged by size:
2634 2007 879 486 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4368
Fragments arranged by size:
4368 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4148 4507 4666 518
Size: 648 2982 359 159 2042
Fragments arranged by size:
2982 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2629 3208 3606 4283 5431 5676 1606
Size: 1023 579 398 677 1148 245 2120
Fragments arranged by size:
2120 1148 1023 677 579 398 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4699
Fragments arranged by size:
4699 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6190
NheI G'CTAG_C
Cuts at: 1052 3142 1052
Size: 2090 4100
NotI GC'GGCC_GC
Cuts at: 3637 3637
Size: 6190
NspI r_CATG'y
Cuts at: 1132 1229 2087 2833 3371 3548 5093 1132
Size: 97 858 746 538 177 1545 2229
Fragments arranged by size:
2229 1545 858 746 538 177 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6190
PciI A'CATG_T
Cuts at: 2083 5089 2083
Size: 3006 3184
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6190
PmeI GTTT'AAAC
Cuts at: 3494 3494
Size: 6190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2247 2279 4259 4291 5772 5804 2247
Size: 32 1980 32 1481 32 2633
Fragments arranged by size:
2633 1980 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6190
PsiI TTA'TAA
Cuts at: 3760 3760
Size: 6190
PstI C_TGCA'G
Cuts at: 830 2533 3542 830
Size: 1703 1009 3478
Fragments arranged by size:
3478 1703 1009
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6190
PvuII CAG'CTG
Cuts at: 2629 3208 2629
Size: 579 5611
SalI G'TCGA_C
Cuts at: 1936 3532 1936
Size: 1596 4594
SapI GCTCTTCn'nnn_
Cuts at: 3237 3237
Size: 6190
SbfI CC_TGCA'GG
Cuts at: 3542 3542
Size: 6190
ScaI AGT'ACT
Cuts at: 2564 4438 6150 2564
Size: 1874 1712 2604
Fragments arranged by size:
2604 1874 1712
SfcI C'TryA_G
Cuts at: 826 1045 2032 2529 2891 3098 3538 4673 5354
Size: 219 987 497 362 207 440 1135 681
Cuts at: 5354 5545 826
Size: 191 1471
Fragments arranged by size:
1471 1135 987 681 497 440 362 219
207 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6190
SmaI CCC'GGG
Cuts at: 1872 3517 1872
Size: 1645 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4294 5195 5457 820
Size: 197 926 2351 901 262 1553
Fragments arranged by size:
2351 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3981 493
Size: 3488 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6190
SphI G_CATG'C
Cuts at: 1132 3371 3548 1132
Size: 2239 177 3774
Fragments arranged by size:
3774 2239 177
SspI AAT'ATT
Cuts at: 5 52 4114 5
Size: 47 4062 2081
Fragments arranged by size:
4062 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6190
StyI C'CwwG_G
Cuts at: 513 1065 2004 3629 513
Size: 552 939 1625 3074
Fragments arranged by size:
3074 1625 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4217 4234 4387 4572 5017 325
Size: 1255 2637 17 153 185 445 1498
Fragments arranged by size:
2637 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2562 4436 6148
Size: 274 80 33 51 1443 585 1874 1712
Cuts at: 6148 96
Size: 138
Fragments arranged by size:
1874 1712 1443 585 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6150 6182 6150
Size: 32 6158
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6190
XbaI T'CTAG_A
Cuts at: 3526 3526
Size: 6190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2488 2488
Size: 6190
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6190
XmaI C'CCGG_G
Cuts at: 1870 3515 1870
Size: 1645 4545
XmnI GAAnn'nnTTC
Cuts at: 2745 4319 2745
Size: 1574 4616
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3998 276
Size: 53 83 186 936 2464 2468
Fragments arranged by size:
2468 2464 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI