Restriction map for
pFN21AB5874
(Circular) (Six-base) MAPSORT of: pFN21AB5874.seq Check: 7788 from: 1 to: 6187
pFN21AB5874 6187 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3997 278
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3530 1937
Size: 1593 4594
Acc65I G'GTAC_C
Cuts at: 3508 3508
Size: 6187
AclI AA'CG_TT
Cuts at: 1563 4314 4687 1563
Size: 2751 373 3063
Fragments arranged by size:
3063 2751 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2123 2514 2844 4250 5634 5902 1632
Size: 491 391 330 1406 1384 268 1917
Fragments arranged by size:
1917 1406 1384 491 391 330 268
AfeI AGC'GCT
Cuts at: 2063 2788 2063
Size: 725 5462
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5990
AflIII A'CryG_T
Cuts at: 1203 5086 6063 1203
Size: 3883 977 1327
Fragments arranged by size:
3883 1327 977
AgeI A'CCGG_T
Cuts at: 5018 5018
Size: 6187
AhdI GACnn_n'nnGTC
Cuts at: 4916 4916
Size: 6187
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6187
AlwNI CAG_nnn'CTG
Cuts at: 3930 5502 3930
Size: 1572 4615
ApaLI G'TGCA_C
Cuts at: 4244 5400 4244
Size: 1156 5031
ApoI r'AATT_y
Cuts at: 1493 2332 2441 3496 3722 3874 5983 1493
Size: 839 109 1055 226 152 2109 1697
Fragments arranged by size:
2109 1697 1055 839 226 152 109
AseI AT'TA_AT
Cuts at: 160 1032 4741 160
Size: 872 3709 1606
Fragments arranged by size:
3709 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
AvaI C'yCGr_G
Cuts at: 1870 1943 2043 3512 1870
Size: 73 100 1469 4545
Fragments arranged by size:
4545 1469 100 73
BamHI G'GATC_C
Cuts at: 3517 3884 3517
Size: 367 5820
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3308 3508 4963 618
Size: 325 217 627 1521 200 1455 1842
Fragments arranged by size:
1842 1521 1455 627 325 217 200
BanII G_rGCy'C
Cuts at: 1391 2681 2753 3506 1391
Size: 1290 72 753 4072
Fragments arranged by size:
4072 1290 753 72
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2532 3201 928
Size: 971 633 669 3914
Fragments arranged by size:
3914 971 669 633
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4378 4412 1903
Size: 34 50 34 2357 34 3678
Fragments arranged by size:
3678 2357 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4079 5289 4079
Size: 1210 4977
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6187
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2628 4798 136
Size: 107 122 71 631 605 956 2170 1525
Fragments arranged by size:
2170 1525 956 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2660 3218 3464 6182 2660
Size: 558 246 2718 2665
Fragments arranged by size:
2718 2665 558 246
BlpI GC'TnA_GC
Cuts at: 3604 3604
Size: 6187
Bme1580I G_kGCm'C
Cuts at: 1790 4248 5404 1790
Size: 2458 1156 2573
Fragments arranged by size:
2573 2458 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6187
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3205 3918 4876
Size: 416 53 83 399 24 1788 713 958
Cuts at: 4876 442
Size: 1753
Fragments arranged by size:
1788 1753 958 713 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6187
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2679 2679
Size: 6187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2111 2545 3367 4847 1239
Size: 872 434 822 1480 2579
Fragments arranged by size:
2579 1480 872 822 434
Bpu10I CC'TnA_GC
Cuts at: 2030 2030
Size: 6187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3463 4312 5177 5475 3463
Size: 849 865 298 4175
Fragments arranged by size:
4175 865 849 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2589 3314 4850 882
Size: 617 1090 725 1536 2219
Fragments arranged by size:
2219 1536 1090 725 617
BsaAI yAC'GTr
Cuts at: 493 1204 3978 493
Size: 711 2774 2702
Fragments arranged by size:
2774 2702 711
BsaBI GATnn'nnATC
Cuts at: 3223 3463 6181 3223
Size: 240 2718 3229
Fragments arranged by size:
3229 2718 240
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2548 3994 4376 5011
Size: 53 83 186 936 1015 1446 382 635
Cuts at: 5011 275
Size: 1451
Fragments arranged by size:
1451 1446 1015 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4620 5018 5292 5439 5850 4620
Size: 398 274 147 411 4957
Fragments arranged by size:
4957 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2321 2351 2701 2731 2899
Size: 30 165 30 918 30 350 30 168
Cuts at: 2899 2929 3311 3341 1178
Size: 30 382 30 4024
Fragments arranged by size:
4024 918 382 350 168 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2938 2948 2951 3104 3145 3303 1186
Size: 1752 10 3 153 41 158 4070
Fragments arranged by size:
4070 1752 158 153 41 10 3
BseYI C'CCAG_C
Cuts at: 2753 5390 5886 2753
Size: 2637 496 3054
Fragments arranged by size:
3054 2637 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2368 1163
Size: 573 632 4982
Fragments arranged by size:
4982 632 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2026 3637 4398 5426 1540
Size: 275 186 25 1611 761 1028 2301
Fragments arranged by size:
2301 1611 1028 761 275 186 25
BsiHKAI G_wGCw'C
Cuts at: 4248 4333 5404 4248
Size: 85 1071 5031
Fragments arranged by size:
5031 1071 85
BsmI GAATG_Cn'
Cuts at: 3307 3698 3791 3307
Size: 391 93 5703
Fragments arranged by size:
5703 391 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2681 2753 3506 4248 4333 5404 1391
Size: 399 891 72 753 742 85 1071 2174
Fragments arranged by size:
2174 1071 891 753 742 399 85 72
BspEI T'CCGG_A
Cuts at: 5850 5850
Size: 6187
BspHI T'CATG_A
Cuts at: 4076 5806 4076
Size: 1730 4457
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3542 844
Size: 2698 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2685 3207 3231 3247 4074 1394
Size: 429 862 522 24 16 827 3507
Fragments arranged by size:
3507 862 827 522 429 24 16
BsrDI GCAATG_nn'
Cuts at: 65 1248 4682 4856 65
Size: 1183 3434 174 1396
Fragments arranged by size:
3434 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2550 4831 5018 1700
Size: 110 740 2281 187 2869
Fragments arranged by size:
2869 2281 740 187 110
BsrGI T'GTAC_A
Cuts at: 96 2016 96
Size: 1920 4267
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6187
BssSI C'ACGA_G
Cuts at: 4247 5259 4247
Size: 1012 5175
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2153 794
Size: 1359 4828
BstBI TT'CG_AA
Cuts at: 4991 4991
Size: 6187
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6187
BstXI CCAn_nnnn'nTGG
Cuts at: 2789 2789
Size: 6187
BstYI r'GATC_y
Cuts at: 1972 2085 2098 2660 2832 3218 3338 3428 3464
Size: 113 13 562 172 386 120 90 36
Cuts at: 3464 3517 3884 4269 4286 5738 5824 5836 6182
Size: 53 367 385 17 1452 86 12 346
Cuts at: 6182 1972
Size: 1977
Fragments arranged by size:
1977 1452 562 386 385 367 346 172
120 113 90 86 53 36 17 13
12
BtgI C'CryG_G
Cuts at: 513 1065 2009 2215 2222 513
Size: 552 944 206 7 4478
Fragments arranged by size:
4478 944 552 206 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2067 487
Size: 683 56 841 4607
Fragments arranged by size:
4607 841 683 56
BtsI GCAGTG_nn'
Cuts at: 2260 3703 4497 4517 2260
Size: 1443 794 20 3930
Fragments arranged by size:
3930 1443 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6187
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6152
DraI TTT'AAA
Cuts at: 3491 3491
Size: 6187
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5194 809
Size: 507 3878 1802
Fragments arranged by size:
3878 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2023 2278 3634
Size: 54 1425 50 275 18 193 255 1356
Cuts at: 3634 4523 6152 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1356 889 275 255 193 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2023 3634 1537
Size: 275 211 1611 4090
Fragments arranged by size:
4090 1611 275 211
EarI CTCTTCn'nnn_
Cuts at: 1353 2820 2972 3071 4117 1353
Size: 1467 152 99 1046 3423
Fragments arranged by size:
3423 1467 1046 152 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2975 4774 5148 5294 387
Size: 1064 138 1386 1799 374 146 1280
Fragments arranged by size:
1799 1386 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2123 2514 2545 2844 3367 4250
Size: 393 479 12 391 31 299 523 883
Cuts at: 4250 4847 5634 5902 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 883 787 597 523 479 393 391
299 268 31 12
EcoO109I rG'GnC_Cy
Cuts at: 3471 3471
Size: 6187
EcoRI G'AATT_C
Cuts at: 3496 3874 3496
Size: 378 5809
FspI TGC'GCA
Cuts at: 4693 4693
Size: 6187
HaeII r_GCGC'y
Cuts at: 1678 2065 2790 5334 1678
Size: 387 725 2544 2531
Fragments arranged by size:
2544 2531 725 387
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2321 2353 2701 2733 2857
Size: 32 135 32 749 32 348 32 124
Cuts at: 2857 2887 2889 2899 2919 2931 3085 3117 3268
Size: 30 2 10 20 12 154 32 151
Cuts at: 3268 3300 3311 3343 4828 4860 4902 4934 1373
Size: 32 11 32 1485 32 42 32 2626
Fragments arranged by size:
2626 1485 749 348 154 151 135 124
42 32 32 32 32 32 32 32
32 32 30 20 12 11 10 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3531 3777 669
Size: 988 281 1593 246 3079
Fragments arranged by size:
3079 1593 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6187
HpaI GTT'AAC
Cuts at: 3777 3777
Size: 6187
KpnI G_GTAC'C
Cuts at: 3512 3512
Size: 6187
MfeI C'AATT_G
Cuts at: 3786 3786
Size: 6187
MluI A'CGCG_T
Cuts at: 6063 6063
Size: 6187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2463 2699 2885 2975 5301 5485 1305
Size: 1158 236 186 90 2326 184 2007
Fragments arranged by size:
2326 2007 1158 236 186 184 90
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4365
Fragments arranged by size:
4365 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4145 4504 4663 518
Size: 648 2979 359 159 2042
Fragments arranged by size:
2979 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2011 2217 2352 3603 4280 5428 5673 1606
Size: 405 206 135 1251 677 1148 245 2120
Fragments arranged by size:
2120 1251 1148 677 405 245 206 135
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5635
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6187
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6187
NotI GC'GGCC_GC
Cuts at: 3634 3634
Size: 6187
NspI r_CATG'y
Cuts at: 1132 1229 2213 3257 3545 5090 1132
Size: 97 984 1044 288 1545 2229
Fragments arranged by size:
2229 1545 1044 984 288 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6187
PciI A'CATG_T
Cuts at: 5086 5086
Size: 6187
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6187
PfoI T'CCnGG_A
Cuts at: 2088 2687 2088
Size: 599 5588
PmeI GTTT'AAAC
Cuts at: 3491 3491
Size: 6187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4256 4288 5769 5801 4256
Size: 32 1481 32 4642
Fragments arranged by size:
4642 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3471 3471
Size: 6187
PshAI GACnn'nnGTC
Cuts at: 1613 2551 1613
Size: 938 5249
PsiI TTA'TAA
Cuts at: 3757 3757
Size: 6187
PstI C_TGCA'G
Cuts at: 830 3539 830
Size: 2709 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6187
PvuII CAG'CTG
Cuts at: 2352 2352
Size: 6187
SacII CC_GC'GG
Cuts at: 2012 2218 2012
Size: 206 5981
SalI G'TCGA_C
Cuts at: 1936 3529 1936
Size: 1593 4594
SapI GCTCTTCn'nnn_
Cuts at: 2972 2972
Size: 6187
SbfI CC_TGCA'GG
Cuts at: 3539 3539
Size: 6187
ScaI AGT'ACT
Cuts at: 4435 6147 4435
Size: 1712 4475
SfcI C'TryA_G
Cuts at: 826 1045 3535 4670 5351 5542 826
Size: 219 2490 1135 681 191 1471
Fragments arranged by size:
2490 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6187
SmaI CCC'GGG
Cuts at: 1872 2045 3514 1872
Size: 173 1469 4545
Fragments arranged by size:
4545 1469 173
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3478 4291 5192 5454 820
Size: 197 926 1535 813 901 262 1553
Fragments arranged by size:
1553 1535 926 901 813 262 197
SnaBI TAC'GTA
Cuts at: 493 3978 493
Size: 3485 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6187
SphI G_CATG'C
Cuts at: 1132 2213 3545 1132
Size: 1081 1332 3774
Fragments arranged by size:
3774 1332 1081
SspI AAT'ATT
Cuts at: 5 52 4111 5
Size: 47 4059 2081
Fragments arranged by size:
4059 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6187
StyI C'CwwG_G
Cuts at: 513 1065 3626 513
Size: 552 2561 3074
Fragments arranged by size:
3074 2561 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2655 3405 4214 4231 4384 4569 5014
Size: 1255 1075 750 809 17 153 185 445
Cuts at: 5014 325
Size: 1498
Fragments arranged by size:
1498 1255 1075 809 750 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2016 2313 4433
Size: 274 80 33 51 1443 39 297 2120
Cuts at: 4433 6145 96
Size: 1712 138
Fragments arranged by size:
2120 1712 1443 297 274 138 80 51
39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3467 3499 6147 6179 3467
Size: 32 2648 32 3475
Fragments arranged by size:
3475 2648 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2849 3325 1316
Size: 1533 476 4178
Fragments arranged by size:
4178 1533 476
XbaI T'CTAG_A
Cuts at: 3425 3461 3523 3425
Size: 36 62 6089
Fragments arranged by size:
6089 62 36
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6187
XmaI C'CCGG_G
Cuts at: 1870 2043 3512 1870
Size: 173 1469 4545
Fragments arranged by size:
4545 1469 173
XmnI GAAnn'nnTTC
Cuts at: 4316 4316
Size: 6187
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3995 276
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI