Restriction map for
pFN21AB5872
(Circular) (Six-base) MAPSORT of: pFN21AB5872.seq Check: 4821 from: 1 to: 5857
pFN21AB5872 5857 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2536 2910 2536
Size: 374 5483
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3667 278
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2367 3200 1937
Size: 430 833 4594
Fragments arranged by size:
4594 833 430
Acc65I G'GTAC_C
Cuts at: 3178 3178
Size: 5857
AclI AA'CG_TT
Cuts at: 1563 3984 4357 1563
Size: 2421 373 3063
Fragments arranged by size:
3063 2421 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2345 2780 3920 5304 5572 1632
Size: 713 435 1140 1384 268 1917
Fragments arranged by size:
1917 1384 1140 713 435 268
AfeI AGC'GCT
Cuts at: 2893 2893
Size: 5857
AflII C'TTAA_G
Cuts at: 820 1017 2882 820
Size: 197 1865 3795
Fragments arranged by size:
3795 1865 197
AflIII A'CryG_T
Cuts at: 1203 4756 5733 1203
Size: 3553 977 1327
Fragments arranged by size:
3553 1327 977
AgeI A'CCGG_T
Cuts at: 4688 4688
Size: 5857
AhdI GACnn_n'nnGTC
Cuts at: 4586 4586
Size: 5857
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5857
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5857
AlwNI CAG_nnn'CTG
Cuts at: 2737 2911 3600 5172 2737
Size: 174 689 1572 3422
Fragments arranged by size:
3422 1572 689 174
ApaI G_GGCC'C
Cuts at: 2239 2239
Size: 5857
ApaLI G'TGCA_C
Cuts at: 2338 2875 3914 5070 2338
Size: 537 1039 1156 3125
Fragments arranged by size:
3125 1156 1039 537
ApoI r'AATT_y
Cuts at: 1493 3166 3392 3544 5653 1493
Size: 1673 226 152 2109 1697
Fragments arranged by size:
2109 1697 1673 226 152
AscI GG'CGCG_CC
Cuts at: 2058 2058
Size: 5857
AseI AT'TA_AT
Cuts at: 160 1032 4411 160
Size: 872 3379 1606
Fragments arranged by size:
3379 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
AvaI C'yCGr_G
Cuts at: 1870 1943 2135 2209 2218 2258 2407 3182 1870
Size: 73 192 74 9 40 149 775 4545
Fragments arranged by size:
4545 775 192 149 74 73 40 9
BamHI G'GATC_C
Cuts at: 2205 3187 3554 2205
Size: 982 367 4508
Fragments arranged by size:
4508 982 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2228 3178 4633 618
Size: 325 217 627 441 950 1455 1842
Fragments arranged by size:
1842 1455 950 627 441 325 217
BanII G_rGCy'C
Cuts at: 1391 2157 2239 2310 3176 1391
Size: 766 82 71 866 4072
Fragments arranged by size:
4072 866 766 82 71
BbeI G_GCGC'C
Cuts at: 2232 2232
Size: 5857
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2768 928
Size: 971 869 4017
Fragments arranged by size:
4017 971 869
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3133 3167 4048 4082 1903
Size: 34 50 34 1112 34 881 34 3678
Fragments arranged by size:
3678 1112 881 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3749 4959 3749
Size: 1210 4647
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5857
BfrBI ATG'CAT
Cuts at: 2392 3095 2392
Size: 703 5154
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4468 136
Size: 107 122 71 631 605 2796 1525
Fragments arranged by size:
2796 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5852 5852
Size: 5857
BlpI GC'TnA_GC
Cuts at: 3274 3274
Size: 5857
Bme1580I G_kGCm'C
Cuts at: 1790 2239 2342 2718 2879 3918 5074 1790
Size: 449 103 376 161 1039 1156 2573
Fragments arranged by size:
2573 1156 1039 449 376 161 103
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5857
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3588 4546 442
Size: 416 53 83 399 24 2171 958 1753
Fragments arranged by size:
2171 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2333 4517 1239
Size: 1094 2184 2579
Fragments arranged by size:
2579 2184 1094
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2141 3982 4847 5145 2141
Size: 1841 865 298 2853
Fragments arranged by size:
2853 1841 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2354 4520 882
Size: 617 855 2166 2219
Fragments arranged by size:
2219 2166 855 617
BsaAI yAC'GTr
Cuts at: 493 1204 3648 493
Size: 711 2444 2702
Fragments arranged by size:
2702 2444 711
BsaBI GATnn'nnATC
Cuts at: 5851 5851
Size: 5857
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2229 3664 4046 4681
Size: 53 83 186 936 696 1435 382 635
Cuts at: 4681 275
Size: 1451
Fragments arranged by size:
1451 1435 936 696 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4290 4688 4962 5109 5520 4290
Size: 398 274 147 411 4627
Fragments arranged by size:
4627 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5632
Fragments arranged by size:
5632 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2111 1186
Size: 925 4932
BseYI C'CCAG_C
Cuts at: 2427 2686 3127 5060 5556 2427
Size: 259 441 1933 496 2728
Fragments arranged by size:
2728 1933 496 441 259
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2429 2508 2972 1163
Size: 573 693 79 464 4048
Fragments arranged by size:
4048 693 573 464 79
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2193 2752 3050 3307 4068 5096
Size: 275 186 192 559 298 257 761 1028
Cuts at: 5096 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 559 298 275 257 192
186
BsiHKAI G_wGCw'C
Cuts at: 2157 2342 2879 3156 3918 4003 5074 2157
Size: 185 537 277 762 85 1071 2940
Fragments arranged by size:
2940 1071 762 537 277 185 85
BsmI GAATG_Cn'
Cuts at: 3095 3368 3461 3095
Size: 273 93 5491
Fragments arranged by size:
5491 273 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2239 2310 2342 2718 2879 3156
Size: 399 367 82 71 32 376 161 277
Cuts at: 3156 3176 3918 4003 5074 1391
Size: 20 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 376 367 277 161
85 82 71 32 20
BspEI T'CCGG_A
Cuts at: 5520 5520
Size: 5857
BspHI T'CATG_A
Cuts at: 3746 5476 3746
Size: 1730 4127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2536 2910 3212 844
Size: 1692 374 302 3489
Fragments arranged by size:
3489 1692 374 302
BsrBI CCG'CTC
Cuts at: 1394 1823 3744 1394
Size: 429 1921 3507
Fragments arranged by size:
3507 1921 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2865 4352 4526 65
Size: 1183 1617 1487 174 1396
Fragments arranged by size:
1617 1487 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2129 2192 2225 4501 4688 1700
Size: 110 319 63 33 2276 187 2869
Fragments arranged by size:
2869 2276 319 187 110 63 33
BsrGI T'GTAC_A
Cuts at: 96 2352 2838 96
Size: 2256 486 3115
Fragments arranged by size:
3115 2256 486
BssHII G'CGCG_C
Cuts at: 1925 2058 1925
Size: 133 5724
BssSI C'ACGA_G
Cuts at: 2168 3917 4929 2168
Size: 1749 1012 3096
Fragments arranged by size:
3096 1749 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2737 794
Size: 1943 3914
BstBI TT'CG_AA
Cuts at: 4661 4661
Size: 5857
BstEII G'GTnAC_C
Cuts at: 1184 2681 1184
Size: 1497 4360
BstYI r'GATC_y
Cuts at: 1972 2205 2907 3187 3554 3939 3956 5408 5494
Size: 233 702 280 367 385 17 1452 86
Cuts at: 5494 5506 5852 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 702 385 367 346 280 233
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2602 2722 2602
Size: 120 5737
BtgI C'CryG_G
Cuts at: 513 1065 2239 2467 513
Size: 552 1174 228 3903
Fragments arranged by size:
3903 1174 552 228
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5118
Fragments arranged by size:
5118 683 56
BtsI GCAGTG_nn'
Cuts at: 3373 4167 4187 3373
Size: 794 20 5043
Fragments arranged by size:
5043 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5857
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5822
DraI TTT'AAA
Cuts at: 3161 3161
Size: 5857
DraIII CAC_nnn'GTG
Cuts at: 2632 2632
Size: 5857
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4864 809
Size: 507 3548 1802
Fragments arranged by size:
3548 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2190 2706
Size: 54 1425 50 275 18 318 42 516
Cuts at: 2706 3304 4193 5822 8
Size: 598 889 1629 43
Fragments arranged by size:
1629 1425 889 598 516 318 275 54
50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 2190 3304 1537
Size: 275 378 1114 4090
Fragments arranged by size:
4090 1114 378 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2291 2937 3035 3787 1353
Size: 938 646 98 752 3423
Fragments arranged by size:
3423 938 752 646 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2362 4444 4818 4964 387
Size: 1064 138 773 2082 374 146 1280
Fragments arranged by size:
2082 1280 1064 773 374 146 138
EcoICRI GAG'CTC
Cuts at: 2155 2155
Size: 5857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2333 2345 2780 3920 4517 5304 5572
Size: 393 701 12 435 1140 597 787 268
Cuts at: 5572 1239
Size: 1524
Fragments arranged by size:
1524 1140 787 701 597 435 393 268
12
EcoO109I rG'GnC_Cy
Cuts at: 2215 2235 2329 2215
Size: 20 94 5743
Fragments arranged by size:
5743 94 20
EcoRI G'AATT_C
Cuts at: 3166 3544 3166
Size: 378 5479
FspI TGC'GCA
Cuts at: 2277 4363 2277
Size: 2086 3771
HaeII r_GCGC'y
Cuts at: 1678 2127 2232 2895 5004 1678
Size: 449 105 663 2109 2531
Fragments arranged by size:
2531 2109 663 449 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2144 2176 2847 2879 4498
Size: 32 135 32 572 32 671 32 1619
Cuts at: 4498 4530 4572 4604 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1619 671 572 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3201 3447 669
Size: 988 281 1263 246 3079
Fragments arranged by size:
3079 1263 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5857
HpaI GTT'AAC
Cuts at: 3447 3447
Size: 5857
KasI G'GCGC_C
Cuts at: 2228 2228
Size: 5857
KpnI G_GTAC'C
Cuts at: 3182 3182
Size: 5857
MfeI C'AATT_G
Cuts at: 3456 3456
Size: 5857
MluI A'CGCG_T
Cuts at: 5733 5733
Size: 5857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4971 5155 1305
Size: 3666 184 2007
Fragments arranged by size:
3666 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4035
Fragments arranged by size:
4035 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3815 4174 4333 518
Size: 648 2649 359 159 2042
Fragments arranged by size:
2649 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2241 2346 2443 2986 3122 3273 3950
Size: 408 227 105 97 543 136 151 677
Cuts at: 3950 5098 5343 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 543 408 245 227 151
136 105 97
NaeI GCC'GGC
Cuts at: 2194 2227 2194
Size: 33 5824
NarI GG'CG_CC
Cuts at: 2229 2229
Size: 5857
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5305
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5857
NgoMIV G'CCGG_C
Cuts at: 2192 2225 2192
Size: 33 5824
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5857
NotI GC'GGCC_GC
Cuts at: 3304 3304
Size: 5857
NruI TCG'CGA
Cuts at: 2938 2938
Size: 5857
NsiI A_TGCA'T
Cuts at: 2394 3097 2394
Size: 703 5154
NspI r_CATG'y
Cuts at: 1132 1229 2353 2995 3215 4760 1132
Size: 97 1124 642 220 1545 2229
Fragments arranged by size:
2229 1545 1124 642 220 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5857
PciI A'CATG_T
Cuts at: 4756 4756
Size: 5857
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2911 1108
Size: 1803 4054
PfoI T'CCnGG_A
Cuts at: 2082 2289 2082
Size: 207 5650
PmeI GTTT'AAAC
Cuts at: 3161 3161
Size: 5857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3926 3958 5439 5471 3926
Size: 32 1481 32 4312
Fragments arranged by size:
4312 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2215 2215
Size: 5857
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5857
PsiI TTA'TAA
Cuts at: 3427 3427
Size: 5857
PspOMI G'GGCC_C
Cuts at: 2235 2235
Size: 5857
PstI C_TGCA'G
Cuts at: 830 2568 2742 3209 830
Size: 1738 174 467 3478
Fragments arranged by size:
3478 1738 467 174
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5857
PvuII CAG'CTG
Cuts at: 2014 2986 2014
Size: 972 4885
SacI G_AGCT'C
Cuts at: 2157 2157
Size: 5857
SacII CC_GC'GG
Cuts at: 2242 2242
Size: 5857
SalI G'TCGA_C
Cuts at: 1936 3199 1936
Size: 1263 4594
SanDI GG'GwC_CC
Cuts at: 2215 2215
Size: 5857
SapI GCTCTTCn'nnn_
Cuts at: 2937 2937
Size: 5857
SbfI CC_TGCA'GG
Cuts at: 3209 3209
Size: 5857
ScaI AGT'ACT
Cuts at: 2957 4105 5817 2957
Size: 1148 1712 2997
Fragments arranged by size:
2997 1712 1148
SexAI A'CCwGG_T
Cuts at: 2677 2677
Size: 5857
SfcI C'TryA_G
Cuts at: 826 1045 2564 2738 3205 4340 5021 5212 826
Size: 219 1519 174 467 1135 681 191 1471
Fragments arranged by size:
1519 1471 1135 681 467 219 191 174
SfoI GGC'GCC
Cuts at: 2230 2230
Size: 5857
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5857
SmaI CCC'GGG
Cuts at: 1872 2211 2220 3184 1872
Size: 339 9 964 4545
Fragments arranged by size:
4545 964 339 9
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2156 2882 3961 4862 5124 820
Size: 197 926 213 726 1079 901 262 1553
Fragments arranged by size:
1553 1079 926 901 726 262 213 197
SnaBI TAC'GTA
Cuts at: 493 3648 493
Size: 3155 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5857
SphI G_CATG'C
Cuts at: 1132 3215 1132
Size: 2083 3774
SspI AAT'ATT
Cuts at: 5 52 3781 5
Size: 47 3729 2081
Fragments arranged by size:
3729 2081 47
StuI AGG'CCT
Cuts at: 1517 2560 1517
Size: 1043 4814
StyI C'CwwG_G
Cuts at: 513 1065 2778 2859 3296 513
Size: 552 1713 81 437 3074
Fragments arranged by size:
3074 1713 552 437 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2660 2664 2700 3884 3901 4054 4239
Size: 1255 1080 4 36 1184 17 153 185
Cuts at: 4239 4684 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1184 1080 445 185 153 36
17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2352 2838 2955
Size: 274 80 33 51 1443 375 486 117
Cuts at: 2955 4103 5815 96
Size: 1148 1712 138
Fragments arranged by size:
1712 1443 1148 486 375 274 138 117
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3053 3085 5817 5849 3053
Size: 32 2732 32 3061
Fragments arranged by size:
3061 2732 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5857
XbaI T'CTAG_A
Cuts at: 3193 3193
Size: 5857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5857
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5857
XmaI C'CCGG_G
Cuts at: 1870 2209 2218 3182 1870
Size: 339 9 964 4545
Fragments arranged by size:
4545 964 339 9
XmnI GAAnn'nnTTC
Cuts at: 3986 3986
Size: 5857
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3665 276
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI
BstXI BstZ17I EcoNI EcoRV FalI FseI FspAI PacI
PmlI PspXI PsrI RsrII SfiI SrfI SwaI