Restriction map for
pFN21AB5871
(Circular) (Six-base) MAPSORT of: pFN21AB5871.seq Check: 2927 from: 1 to: 6097
pFN21AB5871 6097 bp
With 182 enzymes: SgfI *
March 28, 2012 12:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3907 278
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2109 2713 2867 3440 1937
Size: 172 604 154 573 4594
Fragments arranged by size:
4594 604 573 172 154
Acc65I G'GTAC_C
Cuts at: 2598 3418 2598
Size: 820 5277
AclI AA'CG_TT
Cuts at: 1563 4224 4597 1563
Size: 2661 373 3063
Fragments arranged by size:
3063 2661 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2924 4160 5544 5812 1632
Size: 1292 1236 1384 268 1917
Fragments arranged by size:
1917 1384 1292 1236 268
AflII C'TTAA_G
Cuts at: 820 1017 2222 820
Size: 197 1205 4695
Fragments arranged by size:
4695 1205 197
AflIII A'CryG_T
Cuts at: 1203 4996 5973 1203
Size: 3793 977 1327
Fragments arranged by size:
3793 1327 977
AgeI A'CCGG_T
Cuts at: 4928 4928
Size: 6097
AhdI GACnn_n'nnGTC
Cuts at: 4826 4826
Size: 6097
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6097
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6097
AlwNI CAG_nnn'CTG
Cuts at: 2947 3840 5412 2947
Size: 893 1572 3632
Fragments arranged by size:
3632 1572 893
ApaLI G'TGCA_C
Cuts at: 4154 5310 4154
Size: 1156 4941
ApoI r'AATT_y
Cuts at: 1493 3074 3406 3632 3784 5893 1493
Size: 1581 332 226 152 2109 1697
Fragments arranged by size:
2109 1697 1581 332 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2840 4651 160
Size: 872 1808 1811 1606
Fragments arranged by size:
1811 1808 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
AvaI C'yCGr_G
Cuts at: 1870 1943 2376 3422 1870
Size: 73 433 1046 4545
Fragments arranged by size:
4545 1046 433 73
BamHI G'GATC_C
Cuts at: 2575 3427 3794 2575
Size: 852 367 4878
Fragments arranged by size:
4878 852 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2598 3418 4873 618
Size: 325 217 627 811 820 1455 1842
Fragments arranged by size:
1842 1455 820 811 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2608 3416 1391
Size: 1217 808 4072
Fragments arranged by size:
4072 1217 808
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3021 928
Size: 971 1122 4004
Fragments arranged by size:
4004 1122 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4288 4322 1903
Size: 34 2351 34 3678
Fragments arranged by size:
3678 2351 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3335 3989 5199 3335
Size: 654 1210 4233
Fragments arranged by size:
4233 1210 654
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6097
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4708 136
Size: 107 122 71 631 605 3036 1525
Fragments arranged by size:
3036 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6092 6092
Size: 6097
BlpI GC'TnA_GC
Cuts at: 2030 3514 2030
Size: 1484 4613
Bme1580I G_kGCm'C
Cuts at: 1790 4158 5314 1790
Size: 2368 1156 2573
Fragments arranged by size:
2573 2368 1156
BmgBI CAC'GTC
Cuts at: 1318 2107 1318
Size: 789 5308
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2184 2449 3828
Size: 416 53 83 399 24 767 265 1379
Cuts at: 3828 4786 442
Size: 958 1753
Fragments arranged by size:
1753 1379 958 767 416 399 265 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4757 1239
Size: 3518 2579
Bpu10I CC'TnA_GC
Cuts at: 2496 2496
Size: 6097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4222 5087 5385 4222
Size: 865 298 4934
Fragments arranged by size:
4934 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4760 882
Size: 617 3261 2219
Fragments arranged by size:
3261 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2865 3888 493
Size: 711 1661 1023 2702
Fragments arranged by size:
2702 1661 1023 711
BsaBI GATnn'nnATC
Cuts at: 2574 6091 2574
Size: 3517 2580
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3904 4286 4921 275
Size: 53 83 186 936 2371 382 635 1451
Fragments arranged by size:
2371 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4530 4928 5202 5349 5760 4530
Size: 398 274 147 411 4867
Fragments arranged by size:
4867 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5872
Fragments arranged by size:
5872 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6097
BseYI C'CCAG_C
Cuts at: 3357 5300 5796 3357
Size: 1943 496 3658
Fragments arranged by size:
3658 1943 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5524
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3547 4308 5336 1540
Size: 275 186 1546 761 1028 2301
Fragments arranged by size:
2301 1546 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4158 4243 5314 4158
Size: 85 1071 4941
Fragments arranged by size:
4941 1071 85
BsmI GAATG_Cn'
Cuts at: 2927 3608 3701 2927
Size: 681 93 5323
Fragments arranged by size:
5323 681 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2608 3416 4158 4243 5314 1391
Size: 399 818 808 742 85 1071 2174
Fragments arranged by size:
2174 1071 818 808 742 399 85
BspEI T'CCGG_A
Cuts at: 5760 5760
Size: 6097
BspHI T'CATG_A
Cuts at: 3255 3986 5716 3255
Size: 731 1730 3636
Fragments arranged by size:
3636 1730 731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2436 2473 3452 844
Size: 1592 37 979 3489
Fragments arranged by size:
3489 1592 979 37
BsrBI CCG'CTC
Cuts at: 1394 1823 2041 2047 3984 1394
Size: 429 218 6 1937 3507
Fragments arranged by size:
3507 1937 429 218 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 4592 4766 65
Size: 1183 3344 174 1396
Fragments arranged by size:
3344 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3044 4741 4928 1700
Size: 110 1234 1697 187 2869
Fragments arranged by size:
2869 1697 1234 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6097
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6097
BssSI C'ACGA_G
Cuts at: 4157 5169 4157
Size: 1012 5085
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6097
BstBI TT'CG_AA
Cuts at: 4901 4901
Size: 6097
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6097
BstXI CCAn_nnnn'nTGG
Cuts at: 3152 3152
Size: 6097
BstYI r'GATC_y
Cuts at: 1972 2178 2406 2575 3427 3794 4179 4196 5648
Size: 206 228 169 852 367 385 17 1452
Cuts at: 5648 5734 5746 6092 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 852 385 367 346 228 206
169 86 17 12
BstZ17I GTA'TAC
Cuts at: 2868 2868
Size: 6097
Bsu36I CC'TnA_GG
Cuts at: 3241 3241
Size: 6097
BtgI C'CryG_G
Cuts at: 513 1065 2004 2955 513
Size: 552 939 951 3655
Fragments arranged by size:
3655 951 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5358
Fragments arranged by size:
5358 683 56
BtsI GCAGTG_nn'
Cuts at: 3613 4407 4427 3613
Size: 794 20 5283
Fragments arranged by size:
5283 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6097
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6062
DraI TTT'AAA
Cuts at: 2020 2157 3401 2020
Size: 137 1244 4716
Fragments arranged by size:
4716 1244 137
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5104 809
Size: 507 3788 1802
Fragments arranged by size:
3788 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2086 3160 3544
Size: 54 1425 50 275 18 256 1074 384
Cuts at: 3544 4433 6062 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1074 889 384 275 256 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3544 1537
Size: 275 1732 4090
Fragments arranged by size:
4090 1732 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2250 2500 4027 1353
Size: 897 250 1527 3423
Fragments arranged by size:
3423 1527 897 250
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4684 5058 5204 387
Size: 1064 138 3095 374 146 1280
Fragments arranged by size:
3095 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2924 4160 4757 5544 5812 1239
Size: 393 1292 1236 597 787 268 1524
Fragments arranged by size:
1524 1292 1236 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2499 2499
Size: 6097
EcoO109I rG'GnC_Cy
Cuts at: 2056 2492 2998 3197 2056
Size: 436 506 199 4956
Fragments arranged by size:
4956 506 436 199
EcoRI G'AATT_C
Cuts at: 3406 3784 3406
Size: 378 5719
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3038 3038
Size: 6097
FspI TGC'GCA
Cuts at: 4603 4603
Size: 6097
HaeII r_GCGC'y
Cuts at: 1678 5244 1678
Size: 3566 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4738 4770 4812 4844 1373
Size: 32 135 32 3166 32 42 32 2626
Fragments arranged by size:
3166 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2714 3441 3687 669
Size: 988 281 776 727 246 3079
Fragments arranged by size:
3079 988 776 727 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6097
HpaI GTT'AAC
Cuts at: 3687 3687
Size: 6097
KpnI G_GTAC'C
Cuts at: 2602 3422 2602
Size: 820 5277
MfeI C'AATT_G
Cuts at: 3696 3696
Size: 6097
MluI A'CGCG_T
Cuts at: 5973 5973
Size: 6097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5211 5395 1305
Size: 3906 184 2007
Fragments arranged by size:
3906 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3162 10
Size: 54 1425 343 1330 2945
Fragments arranged by size:
2945 1425 1330 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3150 4055 4414 4573 518
Size: 648 1984 905 359 159 2042
Fragments arranged by size:
2042 1984 905 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2758 3513 4190 5338 5583 1606
Size: 1152 755 677 1148 245 2120
Fragments arranged by size:
2120 1152 1148 755 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2955 513
Size: 552 939 951 3655
Fragments arranged by size:
3655 951 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6097
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6097
NotI GC'GGCC_GC
Cuts at: 3544 3544
Size: 6097
NspI r_CATG'y
Cuts at: 1132 1229 2196 2435 3290 3455 5000 1132
Size: 97 967 239 855 165 1545 2229
Fragments arranged by size:
2229 1545 967 855 239 165 97
PasI CC'CwG_GG
Cuts at: 1348 3167 1348
Size: 1819 4278
PciI A'CATG_T
Cuts at: 4996 4996
Size: 6097
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6097
PmeI GTTT'AAAC
Cuts at: 3401 3401
Size: 6097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2298 2330 4166 4198 5679 5711 2298
Size: 32 1836 32 1481 32 2684
Fragments arranged by size:
2684 1836 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2056 2492 2998 3197 2056
Size: 436 506 199 4956
Fragments arranged by size:
4956 506 436 199
PshAI GACnn'nnGTC
Cuts at: 1613 3183 1613
Size: 1570 4527
PsiI TTA'TAA
Cuts at: 3667 3667
Size: 6097
PstI C_TGCA'G
Cuts at: 830 2794 2879 3449 830
Size: 1964 85 570 3478
Fragments arranged by size:
3478 1964 570 85
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6097
PvuII CAG'CTG
Cuts at: 2758 2758
Size: 6097
SalI G'TCGA_C
Cuts at: 1936 2712 3439 1936
Size: 776 727 4594
Fragments arranged by size:
4594 776 727
SanDI GG'GwC_CC
Cuts at: 3197 3197
Size: 6097
SbfI CC_TGCA'GG
Cuts at: 3449 3449
Size: 6097
ScaI AGT'ACT
Cuts at: 3374 4345 6057 3374
Size: 971 1712 3414
Fragments arranged by size:
3414 1712 971
SfcI C'TryA_G
Cuts at: 826 1045 2661 2790 2875 3445 4580 5261 5452
Size: 219 1616 129 85 570 1135 681 191
Cuts at: 5452 826
Size: 1471
Fragments arranged by size:
1616 1471 1135 681 570 219 191 129
85
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6097
SmaI CCC'GGG
Cuts at: 1872 3424 1872
Size: 1552 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2222 4201 5102 5364 820
Size: 197 926 279 1979 901 262 1553
Fragments arranged by size:
1979 1553 926 901 279 262 197
SnaBI TAC'GTA
Cuts at: 493 2865 3888 493
Size: 2372 1023 2702
Fragments arranged by size:
2702 2372 1023
SpeI A'CTAG_T
Cuts at: 152 2732 152
Size: 2580 3517
SphI G_CATG'C
Cuts at: 1132 2435 3455 1132
Size: 1303 1020 3774
Fragments arranged by size:
3774 1303 1020
SspI AAT'ATT
Cuts at: 5 52 2473 2703 2769 4021 5
Size: 47 2421 230 66 1252 2081
Fragments arranged by size:
2421 2081 1252 230 66 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6097
StyI C'CwwG_G
Cuts at: 513 1065 2004 2955 3536 513
Size: 552 939 951 581 3074
Fragments arranged by size:
3074 951 939 581 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2431 2474 3180 4124 4141 4294 4479
Size: 1255 851 43 706 944 17 153 185
Cuts at: 4479 4924 325
Size: 445 1498
Fragments arranged by size:
1498 1255 944 851 706 445 185 153
43 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3372 4343 6055
Size: 274 80 33 51 1443 1395 971 1712
Cuts at: 6055 96
Size: 138
Fragments arranged by size:
1712 1443 1395 971 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6057 6089 6057
Size: 32 6065
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6097
XbaI T'CTAG_A
Cuts at: 3433 3433
Size: 6097
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6097
XmaI C'CCGG_G
Cuts at: 1870 3422 1870
Size: 1552 4545
XmnI GAAnn'nnTTC
Cuts at: 2616 2849 4226 2616
Size: 233 1377 4487
Fragments arranged by size:
4487 1377 233
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3905 276
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI