Restriction map for
pFN21AB5869
(Circular) (Six-base) MAPSORT of: pFN21AB5869.seq Check: 5806 from: 1 to: 6094
pFN21AB5869 6094 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3904 278
Size: 53 83 186 936 2368 2468
Fragments arranged by size:
2468 2368 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2463 3437 1937
Size: 526 974 4594
Fragments arranged by size:
4594 974 526
Acc65I G'GTAC_C
Cuts at: 3415 3415
Size: 6094
AclI AA'CG_TT
Cuts at: 1563 4221 4594 1563
Size: 2658 373 3063
Fragments arranged by size:
3063 2658 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4157 5541 5809 1632
Size: 2525 1384 268 1917
Fragments arranged by size:
2525 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5897
AflIII A'CryG_T
Cuts at: 1203 2523 2525 2998 4993 5970 1203
Size: 1320 2 473 1995 977 1327
Fragments arranged by size:
1995 1327 1320 977 473 2
AgeI A'CCGG_T
Cuts at: 4925 4925
Size: 6094
AhdI GACnn_n'nnGTC
Cuts at: 4823 4823
Size: 6094
AleI CACnn'nnGTG
Cuts at: 1166 2418 1166
Size: 1252 4842
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6094
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2369 2401 2369
Size: 32 6062
AlwNI CAG_nnn'CTG
Cuts at: 3837 5409 3837
Size: 1572 4522
ApaI G_GGCC'C
Cuts at: 2240 2240
Size: 6094
ApaLI G'TGCA_C
Cuts at: 4151 5307 4151
Size: 1156 4938
ApoI r'AATT_y
Cuts at: 1493 3403 3629 3781 5890 1493
Size: 1910 226 152 2109 1697
Fragments arranged by size:
2109 1910 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4648 160
Size: 872 3616 1606
Fragments arranged by size:
3616 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
AvaI C'yCGr_G
Cuts at: 1870 1943 2360 3419 1870
Size: 73 417 1059 4545
Fragments arranged by size:
4545 1059 417 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2136 2169 2136
Size: 33 6061
BamHI G'GATC_C
Cuts at: 3424 3791 3424
Size: 367 5727
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3415 4870 618
Size: 325 217 627 1628 1455 1842
Fragments arranged by size:
1842 1628 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2090 2207 2240 3413 1391
Size: 699 117 33 1173 4072
Fragments arranged by size:
4072 1173 699 117 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2706 928
Size: 971 807 4316
Fragments arranged by size:
4316 971 807
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4285 4319 1903
Size: 34 2348 34 3678
Fragments arranged by size:
3678 2348 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3240 3986 5196 3240
Size: 746 1210 4138
Fragments arranged by size:
4138 1210 746
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6094
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2070 4705 136
Size: 107 122 71 631 605 398 2635 1525
Fragments arranged by size:
2635 1525 631 605 398 122 107 71
BglII A'GATC_T
Cuts at: 6089 6089
Size: 6094
BlpI GC'TnA_GC
Cuts at: 3511 3511
Size: 6094
Bme1580I G_kGCm'C
Cuts at: 1790 2161 2240 3019 4155 5311 1790
Size: 371 79 779 1136 1156 2573
Fragments arranged by size:
2573 1156 1136 779 371 79
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6094
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2144 3116 3825
Size: 416 53 83 399 24 727 972 709
Cuts at: 3825 4783 442
Size: 958 1753
Fragments arranged by size:
1753 972 958 727 709 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6094
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2250 3326 4754 1239
Size: 1011 1076 1428 2579
Fragments arranged by size:
2579 1428 1076 1011
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3227 4219 5084 5382 3227
Size: 992 865 298 3939
Fragments arranged by size:
3939 992 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3368 4757 882
Size: 617 1869 1389 2219
Fragments arranged by size:
2219 1869 1389 617
BsaAI yAC'GTr
Cuts at: 493 1204 2526 3885 493
Size: 711 1322 1359 2702
Fragments arranged by size:
2702 1359 1322 711
BsaBI GATnn'nnATC
Cuts at: 6088 6088
Size: 6094
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3901 4283 4918 275
Size: 53 83 186 936 2368 382 635 1451
Fragments arranged by size:
2368 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3006 3232 4527 4925 5199 5346 5757 3006
Size: 226 1295 398 274 147 411 3343
Fragments arranged by size:
3343 1295 411 398 274 226 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5869
Fragments arranged by size:
5869 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2662 1186
Size: 1476 4618
BseYI C'CCAG_C
Cuts at: 2132 2291 3026 3274 5297 5793 2132
Size: 159 735 248 2023 496 2433
Fragments arranged by size:
2433 2023 735 496 248 159
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2196 2987 1163
Size: 573 460 791 4270
Fragments arranged by size:
4270 791 573 460
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3544 4305 5333 1540
Size: 275 186 1543 761 1028 2301
Fragments arranged by size:
2301 1543 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2254 3312 4155 4240 5311 2254
Size: 1058 843 85 1071 3037
Fragments arranged by size:
3037 1071 1058 843 85
BsmI GAATG_Cn'
Cuts at: 3605 3698 3605
Size: 93 6001
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2161 2207 2240 2254 3019 3312
Size: 399 300 71 46 33 14 765 293
Cuts at: 3312 3413 4155 4240 5311 1391
Size: 101 742 85 1071 2174
Fragments arranged by size:
2174 1071 765 742 399 300 293 101
85 71 46 33 14
BspEI T'CCGG_A
Cuts at: 3232 5757 3232
Size: 2525 3569
BspHI T'CATG_A
Cuts at: 2028 2695 3983 5713 2028
Size: 667 1288 1730 2409
Fragments arranged by size:
2409 1730 1288 667
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3237 3449 844
Size: 2393 212 3489
Fragments arranged by size:
3489 2393 212
BsrBI CCG'CTC
Cuts at: 1394 1823 2955 3981 1394
Size: 429 1132 1026 3507
Fragments arranged by size:
3507 1132 1026 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4589 4763 65
Size: 1183 3341 174 1396
Fragments arranged by size:
3341 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4738 4925 1700
Size: 110 2928 187 2869
Fragments arranged by size:
2928 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6094
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6094
BssSI C'ACGA_G
Cuts at: 4154 5166 4154
Size: 1012 5082
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2574 2681 794
Size: 1780 107 4207
Fragments arranged by size:
4207 1780 107
BstBI TT'CG_AA
Cuts at: 4898 4898
Size: 6094
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6094
BstXI CCAn_nnnn'nTGG
Cuts at: 3026 3026
Size: 6094
BstYI r'GATC_y
Cuts at: 1972 2399 2438 3424 3791 4176 4193 5645 5731
Size: 427 39 986 367 385 17 1452 86
Cuts at: 5731 5743 6089 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 986 427 385 367 346 86
39 17 12
BstZ17I GTA'TAC
Cuts at: 2464 2464
Size: 6094
Bsu36I CC'TnA_GG
Cuts at: 2245 2443 2245
Size: 198 5896
BtgI C'CryG_G
Cuts at: 513 1065 2162 2232 2988 513
Size: 552 1097 70 756 3619
Fragments arranged by size:
3619 1097 756 552 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5355
Fragments arranged by size:
5355 683 56
BtsI GCAGTG_nn'
Cuts at: 2184 3019 3610 4404 4424 2184
Size: 835 591 794 20 3854
Fragments arranged by size:
3854 835 794 591 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6094
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6059
DraI TTT'AAA
Cuts at: 3398 3398
Size: 6094
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5101 809
Size: 507 3785 1802
Fragments arranged by size:
3785 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2062 2985 3541
Size: 54 1425 50 275 18 232 923 556
Cuts at: 3541 4430 6059 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 923 889 556 275 232 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3541 1537
Size: 275 1729 4090
Fragments arranged by size:
4090 1729 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2612 3320 4024 1353
Size: 1259 708 704 3423
Fragments arranged by size:
3423 1259 708 704
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4681 5055 5201 387
Size: 1064 138 3092 374 146 1280
Fragments arranged by size:
3092 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2250 3326 4157 4754 5541 5809 1239
Size: 393 618 1076 831 597 787 268 1524
Fragments arranged by size:
1524 1076 831 787 618 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2184 2512 3151 2184
Size: 328 639 5127
Fragments arranged by size:
5127 639 328
EcoRI G'AATT_C
Cuts at: 3403 3781 3403
Size: 378 5716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2750 2750
Size: 6094
FspI TGC'GCA
Cuts at: 4600 4600
Size: 6094
HaeII r_GCGC'y
Cuts at: 1678 2264 5241 1678
Size: 586 2977 2531
Fragments arranged by size:
2977 2531 586
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2426 2458 4735 4767 4809
Size: 32 135 32 854 32 2277 32 42
Cuts at: 4809 4841 1373
Size: 32 2626
Fragments arranged by size:
2626 2277 854 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2314 3438 3684 669
Size: 988 281 376 1124 246 3079
Fragments arranged by size:
3079 1124 988 376 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6094
HpaI GTT'AAC
Cuts at: 3684 3684
Size: 6094
KpnI G_GTAC'C
Cuts at: 3419 3419
Size: 6094
MfeI C'AATT_G
Cuts at: 3693 3693
Size: 6094
MluI A'CGCG_T
Cuts at: 5970 5970
Size: 6094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3081 3183 3351 5208 5392 1305
Size: 1776 102 168 1857 184 2007
Fragments arranged by size:
2007 1857 1776 184 168 102
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2064 2987 10
Size: 54 1425 343 232 923 3117
Fragments arranged by size:
3117 1425 923 343 232 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2418 2612 2708 2774 3089 3365 4052
Size: 648 1252 194 96 66 315 276 687
Cuts at: 4052 4411 4570 518
Size: 359 159 2042
Fragments arranged by size:
2042 1252 687 648 359 315 276 194
159 96 66
MspA1I CmG'CkG
Cuts at: 1606 2574 3382 3510 4187 5335 5580 1606
Size: 968 808 128 677 1148 245 2120
Fragments arranged by size:
2120 1148 968 808 677 245 128
NcoI C'CATG_G
Cuts at: 513 1065 2232 513
Size: 552 1167 4375
Fragments arranged by size:
4375 1167 552
NdeI CA'TA_TG
Cuts at: 387 2810 387
Size: 2423 3671
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6094
NotI GC'GGCC_GC
Cuts at: 3541 3541
Size: 6094
NspI r_CATG'y
Cuts at: 1132 1229 2707 3002 3088 3452 4997 1132
Size: 97 1478 295 86 364 1545 2229
Fragments arranged by size:
2229 1545 1478 364 295 97 86
PasI CC'CwG_GG
Cuts at: 1348 2200 1348
Size: 852 5242
PciI A'CATG_T
Cuts at: 2998 4993 2998
Size: 1995 4099
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6094
PfoI T'CCnGG_A
Cuts at: 2266 2266
Size: 6094
PmeI GTTT'AAAC
Cuts at: 3398 3398
Size: 6094
PmlI CAC'GTG
Cuts at: 2526 2526
Size: 6094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4163 4195 5676 5708 4163
Size: 32 1481 32 4549
Fragments arranged by size:
4549 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2184 2512 2184
Size: 328 5766
PshAI GACnn'nnGTC
Cuts at: 1613 2559 1613
Size: 946 5148
PsiI TTA'TAA
Cuts at: 3664 3664
Size: 6094
PspOMI G'GGCC_C
Cuts at: 2236 2236
Size: 6094
PstI C_TGCA'G
Cuts at: 830 3387 3446 830
Size: 2557 59 3478
Fragments arranged by size:
3478 2557 59
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6094
SalI G'TCGA_C
Cuts at: 1936 3436 1936
Size: 1500 4594
SanDI GG'GwC_CC
Cuts at: 2184 2184
Size: 6094
SbfI CC_TGCA'GG
Cuts at: 3446 3446
Size: 6094
ScaI AGT'ACT
Cuts at: 2582 4342 6054 2582
Size: 1760 1712 2622
Fragments arranged by size:
2622 1760 1712
SexAI A'CCwGG_T
Cuts at: 2895 2895
Size: 6094
SfcI C'TryA_G
Cuts at: 826 1045 3383 3442 4577 5258 5449 826
Size: 219 2338 59 1135 681 191 1471
Fragments arranged by size:
2338 1471 1135 681 219 191 59
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6094
SmaI CCC'GGG
Cuts at: 1872 2362 3421 1872
Size: 490 1059 4545
Fragments arranged by size:
4545 1059 490
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3206 4198 5099 5361 820
Size: 197 926 1263 992 901 262 1553
Fragments arranged by size:
1553 1263 992 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3885 493
Size: 3392 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6094
SphI G_CATG'C
Cuts at: 1132 3452 1132
Size: 2320 3774
SspI AAT'ATT
Cuts at: 5 52 4018 5
Size: 47 3966 2081
Fragments arranged by size:
3966 2081 47
StuI AGG'CCT
Cuts at: 1517 3212 1517
Size: 1695 4399
StyI C'CwwG_G
Cuts at: 513 1065 2232 2886 3147 3533 513
Size: 552 1167 654 261 386 3074
Fragments arranged by size:
3074 1167 654 552 386 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2305 4121 4138 4291 4476 4921 325
Size: 1255 725 1816 17 153 185 445 1498
Fragments arranged by size:
1816 1498 1255 725 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2580 3127 4340
Size: 274 80 33 51 1443 603 547 1213
Cuts at: 4340 6052 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1213 603 547 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2158 2190 6054 6086 2158
Size: 32 3864 32 2166
Fragments arranged by size:
3864 2166 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2897 1316
Size: 1581 4513
XbaI T'CTAG_A
Cuts at: 2722 3430 2722
Size: 708 5386
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6094
XmaI C'CCGG_G
Cuts at: 1870 2360 3419 1870
Size: 490 1059 4545
Fragments arranged by size:
4545 1059 490
XmnI GAAnn'nnTTC
Cuts at: 2017 4223 2017
Size: 2206 3888
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3902 276
Size: 53 83 186 936 2368 2468
Fragments arranged by size:
2468 2368 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PspXI PsrI PvuII RsrII SacI SacII SapI SfiI
SfoI SrfI SwaI XcmI